| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22401-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37276-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-42191-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05183-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06323-50ul | |||
![Western Blot: OLIG1 Antibody [NBP1-28666] - Neonatal rat brain lysate showing specific immunolabeling of the ~ 27k Olig1 protein.](http://images.novusbio.com/fullsize/OLIG1-Antibody-Western-Blot-NBP1-28666-img0001.jpg "Western Blot: OLIG1 Antibody [NBP1-28666] - Neonatal rat brain lysate showing specific immunolabeling of the ~ 27k Olig1 protein.")
![Immunocytochemistry/Immunofluorescence: Olig1 Antibody [NBP1-28666] - Immunostaining of primary rat oligodendrocytes using anti-Olig1.](http://images.novusbio.com/fullsize/Olig1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-28666-img0002.jpg)
![Western Blot: Brevican Antibody (S294A-6) [NBP2-22401] - Western Blot analysis of Rat Brain Membrane showing detection of ~140 kDa Brevican protein using Mouse Anti-Brevican Monoclonal Antibody, Clone S294A-6 (NBP2-22401). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 ug). . Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody (NBP2-22401) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~140 kDa.](http://images.novusbio.com/fullsize/Brevican-Antibody-S294A-6-Western-Blot-NBP2-22401-img0003.jpg "Western Blot: Brevican Antibody (S294A-6) [NBP2-22401] - Western Blot analysis of Rat Brain Membrane showing detection of ~140 kDa Brevican protein using Mouse Anti-Brevican Monoclonal Antibody, Clone S294A-6 (NBP2-22401). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 ug). . Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody (NBP2-22401) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~140 kDa.")
![Immunocytochemistry/Immunofluorescence: Brevican Antibody (S294A-6) [NBP2-22401] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Brevican Monoclonal Antibody, Clone S294A-6 (NBP2-22401). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody (NBP2-22401) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Brevican Antibody (D) Composite.](http://images.novusbio.com/fullsize/Brevican-Antibody-S294A-6-Immunocytochemistry-Immunofluorescence-NBP2-22401-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Brevican Antibody (S294A-6) [NBP2-22401] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/Brevican-Antibody-S294A-6-Immunocytochemistry-Immunofluorescence-NBP2-22401-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Brevican Antibody (S294A-6) [NBP2-22401] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Brevican Monoclonal Antibody, Clone S294A-6 (NBP2-22401). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody (NBP2-22401) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Brevican Antibody. (D) Composite.](http://images.novusbio.com/fullsize/Brevican-Antibody-S294A-6-Immunocytochemistry-Immunofluorescence-NBP2-22401-img0005.jpg)
![Western Blot: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis using BDNF mouse mAb against SK-N-SH (1) cell lysate.](http://images.novusbio.com/fullsize/BDNF-Antibody-3D9C5-Western-Blot-NBP2-37276-img0004.jpg "Western Blot: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis using BDNF mouse mAb against SK-N-SH (1) cell lysate.")
![Immunocytochemistry/Immunofluorescence: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis of Hela cells using BDNF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/BDNF-Antibody-3D9C5-Immunocytochemistry-Immunofluorescence-NBP2-37276-img0005.jpg)
![Western Blot: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis using BDNF mAb against human BDNF (AA: 19-248) recombinant protein. (Expected MW is 51.7 kDa)](http://images.novusbio.com/fullsize/BDNF-Antibody-3D9C5-Western-Blot-NBP2-37276-img0002.jpg)
![Western Blot: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis using BDNF mAb against HEK293 (1) and BDNF (AA: 19-248)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/BDNF-Antibody-3D9C5-Western-Blot-NBP2-37276-img0003.jpg)
![ELISA: BDNF Antibody (3D9C5) [NBP2-37276] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/BDNF-Antibody-3D9C5-ELISA-NBP2-37276-img0001.jpg)
![Western Blot: NrCAM Antibody (S364-51) [NBP2-42191] - Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa NrCam protein using Mouse Anti-NrCam Monoclonal Antibody, Clone S364-51 (NBP2-42191). Load: 10 ug. Primary Antibody: Mouse Anti-NrCam Monoclonal Antibody (NBP2-42191) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~160 kDa.](http://images.novusbio.com/fullsize/NrCAM-Antibody-S364-51-Western-Blot-NBP2-42191-img0003.jpg "Western Blot: NrCAM Antibody (S364-51) [NBP2-42191] - Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa NrCam protein using Mouse Anti-NrCam Monoclonal Antibody, Clone S364-51 (NBP2-42191). Load: 10 ug. Primary Antibody: Mouse Anti-NrCam Monoclonal Antibody (NBP2-42191) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~160 kDa.")
![Immunocytochemistry/Immunofluorescence: NrCAM Antibody (S364-51) [NBP2-42191] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NrCAM Monoclonal Antibody, Clone S364-51 (NBP2-42191). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-NrCAM Monoclonal Antibody (NBP2-42191) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) NrCAM Antibody (D) Composite.](http://images.novusbio.com/fullsize/NrCAM-Antibody-S364-51-Immunocytochemistry-Immunofluorescence-NBP2-42191-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: NrCAM Antibody (S364-51) [NBP2-42191] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NrCAM Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize/NrCAM-Antibody-S364-51-Immunocytochemistry-Immunofluorescence-NBP2-42191-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NrCAM Antibody (S364-51) [NBP2-42191] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NrCAM Monoclonal Antibody, Clone S364-51 (NBP2-42191). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NrCAM Monoclonal Antibody (NBP2-42191) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) NrCAM Antibody. (D) Composite.](http://images.novusbio.com/fullsize/NrCAM-Antibody-S364-51-Immunocytochemistry-Immunofluorescence-NBP2-42191-img0005.jpg)
![Western Blot: Hyaluronan synthase 1 Antibody (3E10) [NBP1-51635] - Analysis using HAS1 mAb against human HAS1 (AA: 74-243) recombinant protein. (Expected MW is 44.4 kDa)](http://images.novusbio.com/fullsize/Hyaluronan-synthase-1-Antibody-3E10-Western-Blot-NBP1-51635-img0007.jpg "Western Blot: Hyaluronan synthase 1 Antibody (3E10) [NBP1-51635] - Analysis using HAS1 mAb against human HAS1 (AA: 74-243) recombinant protein. (Expected MW is 44.4 kDa)")
![Immunocytochemistry/Immunofluorescence: Hyaluronan synthase 1 Antibody (3E10) [NBP1-51635] - Analysis of U251 cells using HAS1 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/Hyaluronan-synthase-1-Antibody-3E10-Immunocytochemistry-Immunofluorescence-NBP1-51635-img0005.jpg)
![Western Blot: Doublecortin Antibody (OTI4A3) - Azide and BSA Free [NBP2-70558] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Doublecortin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Doublecor](http://images.novusbio.com/fullsize/Doublecortin-Antibody-OTI4A3-Azide-and-BSA-Free-Western-Blot-NBP2-70558-img0001.jpg "Western Blot: Doublecortin Antibody (OTI4A3) - Azide and BSA Free [NBP2-70558] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Doublecortin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Doublecor")
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (OTI4A3) - Azide and BSA Free [NBP2-70558] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Doublecortin.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-OTI4A3-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70558-img0003.jpg)
![Flow Cytometry: Doublecortin Antibody (OTI4A3) - Azide and BSA Free [NBP2-70558] - Analysis of Jurkat cells, using anti-Doublecortin antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Doublecortin-Antibody-OTI4A3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70558-img0002.jpg)
![Immunohistochemistry: Lipocalin-2/NGAL Antibody (29) - BSA Free [NBP1-05183] - NGAL expression 2 days after the introduction of ischemic-reperfusion injured mouse kidney;detected with anti-mouse NGAL antibody at dilution of 1:1000. Image and data supplied by Prof. Gueler, Medizinische Hochschule Hannover.](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-29-BSA-Free-Immunohistochemistry-NBP1-05183-img0006.jpg "Immunohistochemistry: Lipocalin-2/NGAL Antibody (29) - BSA Free [NBP1-05183] - NGAL expression 2 days after the introduction of ischemic-reperfusion injured mouse kidney;detected with anti-mouse NGAL antibody at dilution of 1:1000. Image and data supplied by Prof. Gueler, Medizinische Hochschule Hannover.")
![Immunohistochemistry: Lipocalin-2/NGAL Antibody (29) - BSA Free [NBP1-05183] - NGAL expression in the ischemia-reperfusion injured mouse kidney; detected with anti-mouse NGAL antibody at dilution of 1:1000. Image and data supplied by Prof. Gueler, Medizinische Hochschule Hannover.](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-29-BSA-Free-Immunohistochemistry-NBP1-05183-img0005.jpg)
![ELISA: Lipocalin-2/NGAL Antibody (29) - BSA Free [NBP1-05183] - The figure illustrates the calibration curve of a sandwich assay with NBP2-21746 as the capture antibody and NBP1-05183B as the biotinylated detection antibody.](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-29-BSA-Free-ELISA-NBP1-05183-img0007.jpg)
![Western Blot: KIF3A Antibody (JE31-39) [NBP3-32501] - Western blot analysis of KIF3A on different lysates with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Rat brain tissue lysate (40 ug/Lane) Lane 3: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 80 kDa Observed band size: 80 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32501) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32501_rabbit-kif3a-mab-je31-39-278202415295563.jpg "Western Blot: KIF3A Antibody (JE31-39) [NBP3-32501] - Western blot analysis of KIF3A on different lysates with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Rat brain tissue lysate (40 ug/Lane) Lane 3: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 80 kDa Observed band size: 80 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32501) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: KIF3A Antibody (JE31-39) [NBP3-32501] - Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32501) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32501_rabbit-kif3a-mab-je31-39-298202413585569.jpg)
![Immunocytochemistry/ Immunofluorescence: KIF3A Antibody (JE31-39) [NBP3-32501] - Immunocytochemistry analysis of NIH/3T3 cells labeling KIF3A with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32501_rabbit-kif3a-mab-je31-39-29202410334094.jpg)
![Western Blot: CREB3L2 Antibody [NBP2-87208] - Host: Rabbit. Target Name: CREB3L2. Sample Type: Human Fetal Muscle. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/CREB3L2-Antibody-Western-Blot-NBP2-87208-img0005.jpg "Western Blot: CREB3L2 Antibody [NBP2-87208] - Host: Rabbit. Target Name: CREB3L2. Sample Type: Human Fetal Muscle. Antibody Dilution: 1.0ug/ml")
![Immunohistochemistry: CREB3L2 Antibody [NBP2-87208] - Human kidney](http://images.novusbio.com/fullsize/CREB3L2-Antibody-Immunohistochemistry-NBP2-87208-img0001.jpg)
![Western Blot: CREB3L2 Antibody [NBP2-87208] - WB Suggested Anti-CREB3L2 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:12500. Positive Control: Jurkat cell lysate](http://images.novusbio.com/fullsize/CREB3L2-Antibody-Western-Blot-NBP2-87208-img0002.jpg)
![Western Blot: CREB3L2 Antibody [NBP2-87208] - Host: Rabbit. Target Name: CREB3L2. Sample Type: Human Fetal Brain. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/CREB3L2-Antibody-Western-Blot-NBP2-87208-img0003.jpg)
![Western Blot: CREB3L2 Antibody [NBP2-87208] - Host: Rabbit. Target Name: CREB3L2. Sample Type: Human Fetal Heart. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/CREB3L2-Antibody-Western-Blot-NBP2-87208-img0004.jpg)
![Western Blot: Vimentin Antibody (A6-C1-R) [NBP3-33092] - Western blot analysis of Vimentin on different lysates with Mouse anti-Vimentin antibody (NBP3-33092) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: C2C12 cell lysate Lane 3: L6 cell lysate Lane 4: Daudi cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33092) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33092_mouse-vimentin-mab-a6-c1-r-288202414191534.jpg "Western Blot: Vimentin Antibody (A6-C1-R) [NBP3-33092] - Western blot analysis of Vimentin on different lysates with Mouse anti-Vimentin antibody (NBP3-33092) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: C2C12 cell lysate Lane 3: L6 cell lysate Lane 4: Daudi cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33092) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Vimentin Antibody (A6-C1-R) [NBP3-33092] - Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-Vimentin antibody (NBP3-33092) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33092) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33092_mouse-vimentin-mab-a6-c1-r-298202414304186.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody (A6-C1-R) [NBP3-33092] - Immunocytochemistry analysis of HeLa cells labeling Vimentin with Mouse anti-Vimentin antibody (NBP3-33092) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Vimentin antibody (NBP3-33092) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33092_mouse-vimentin-mab-a6-c1-r-2920241192398.jpg)
![Western Blot: ALDH1A2 Antibody [NBP3-06323] - Anti-ALDH1A2 rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:57 kDaObserved band size:55 kDa](http://images.novusbio.com/fullsize/ALDH1A2-Antibody-Western-Blot-NBP3-06323-img0001.jpg "Western Blot: ALDH1A2 Antibody [NBP3-06323] - Anti-ALDH1A2 rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:57 kDaObserved band size:55 kDa")
![Immunoprecipitation: ALDH1A2 Antibody [NBP3-06323] - ALDH1A2 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell Lysate4 uL anti-ALDH1A2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG.Primary antibody:Anti-ALDH1A2 rabbit polyclonal antibody, at 1:100 dilution.Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 57 kDaObserved band size :55 kDa](http://images.novusbio.com/fullsize/ALDH1A2-Antibody-Immunoprecipitation-NBP3-06323-img0002.jpg)
![Immunohistochemistry-Paraffin: ALDH1A2 Antibody - BSA Free [NBP3-06323] - Immunochemical staining of human ALDH1A2 in human kidney with NBP3-06323 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-06323_rabbit-polyclonal-aldh1a2-antibody-23520251628136.png)
![Immunohistochemistry-Paraffin: ALDH1A2 Antibody - BSA Free [NBP3-06323] - Immunochemical staining of human ALDH1A2 in human corpus uteri with NBP3-06323 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-06323_rabbit-polyclonal-aldh1a2-antibody-23520251628123.png)
![Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunofluorescence analysis of paraffin-embedded human lymph nodes tissue labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32498, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-298202413585592.jpg "Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunofluorescence analysis of paraffin-embedded human lymph nodes tissue labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32498, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32498) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-29820241435258.jpg)
![Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunocytochemistry analysis of HeLa cells labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67/MKI67 antibody (NBP3-32498) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-2920241192339.jpg)