| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-216-0.125ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92916-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83162-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-220-0.025ml | |||
![Western Blot: alpha-Internexin Antibody (1D2) [NB300-216] - Analysis of different tissue lysates using alpha-Internexin antibody, dilution 1:10,000 (Green): [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig spinal cord and [7] cow spinal cord. The alpha-Internexin antibody reveals the alpha-internexin protein with apparent molecular weight of 64 to 66 kDa with slight variability among species.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-1D2-Western-Blot-NB300-216-img0002.jpg "Western Blot: alpha-Internexin Antibody (1D2) [NB300-216] - Analysis of different tissue lysates using alpha-Internexin antibody, dilution 1:10,000 (Green): [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig spinal cord and [7] cow spinal cord. The alpha-Internexin antibody reveals the alpha-internexin protein with apparent molecular weight of 64 to 66 kDa with slight variability among species.")
![Immunocytochemistry/Immunofluorescence: alpha Internexin Antibody (1D2) [NB300-216] - Mixed cultures of rat CNS cells stained with NB300-216 (red) and chicken antibody to Microtubule associated protein 2 (MAP2- green). The a-internexin is localized primarily in neuronal axons in these cultures, while the perikarya and dendrites of neurons stain strongly for MAP2.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-1D2-Immunocytochemistry-Immunofluorescence-NB300-216-img0001.jpg)
![Immunohistochemistry Free-Floating: alpha-Internexin Antibody (1D2) [NB300-216] - Analysis of rat cerebellum section stained with alpha-internexin antibody, dilution 1:5,000 (Green), and costained with chicken Calretinin pAb, dilution 1:2,000 (Red). Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with the above antibodies. The alpha-internexin antibody selectively stains neuronal processes, in particular parallel fibers, the axons of granule cells. Calretinin antibody stains interneurons predominantly in the molecular layer of the cerebellum.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-1D2-Immunohistochemistry-Free-Floating-NB300-216-img0003.jpg)
![Western Blot: Dysbindin Antibody [NBP2-16245] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Dysbindin antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Dysbindin-Antibody-Western-Blot-NBP2-16245-img0005.jpg "Western Blot: Dysbindin Antibody [NBP2-16245] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Dysbindin antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Immunohistochemistry-Paraffin: Dysbindin Antibody [NBP2-16245] - Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Dysbindin-Antibody-Immunohistochemistry-Paraffin-NBP2-16245-img0002.jpg)
![Western Blot: Dysbindin Antibody [NBP2-16245] - Sample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 C: U87-MG D: SK-N-SH 10% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/fullsize/Dysbindin-Antibody-Western-Blot-NBP2-16245-img0001.jpg)
![Dysbindin antibody detects Dysbindin protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Dysbindin stained by Dysbindin antibody (NBP2-16245) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-16245_rabbit-polyclonal-dysbindin-antibody-immunocytochemistry-immunofluorescence-2611202518301219.jpg)
![Western Blot: Syntaxin 4 Antibody (6D1) [H00006810-M04] - Analysis of STX4A expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-6D1-Western-Blot-H00006810-M04-img0011.jpg "Western Blot: Syntaxin 4 Antibody (6D1) [H00006810-M04] - Analysis of STX4A expression in NIH/3T3 (Cat # L018V1).")
![Immunocytochemistry/Immunofluorescence: Syntaxin 4 Antibody (6D1) [H00006810-M04] - Analysis of monoclonal antibody to STX4A on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-6D1-Immunocytochemistry-Immunofluorescence-H00006810-M04-img0007.jpg)
![Western Blot: Syntaxin 4 Antibody (6D1) [H00006810-M04] - Analysis of STX4A expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-6D1-Western-Blot-H00006810-M04-img0009.jpg)
![Western Blot: Syntaxin 4 Antibody (6D1) [H00006810-M04] - Analysis of STX4A expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-6D1-Western-Blot-H00006810-M04-img0010.jpg)
![Western Blot: Syntaxin 4 Antibody (6D1) [H00006810-M04] - Analysis of STX4 expression in transfected 293T cell line by STX4A monoclonal antibody (M04), clone 6D1. Lane 1: STX4 transfected lysate (Predicted MW: 34.2 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-6D1-Western-Blot-H00006810-M04-img0012.jpg)
![Sandwich ELISA: Syntaxin 4 Antibody (6D1) [H00006810-M04] - Detection limit for recombinant GST tagged STX4A is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-6D1-Sandwich-ELISA-H00006810-M04-img0008.jpg)
![Western Blot: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SPG7 antibody [HL2236] (NBP3-25694) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25694_rabbit-spg7-mab-hl2236-azide-and-bsa-free-2120249415947.jpg "Western Blot: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SPG7 antibody [HL2236] (NBP3-25694) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] - SPG7 antibody [HL2236] detects SPG7 protein at mitochondria by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: SPG7 stained by SPG7 antibody [HL2236] (NBP3-25694) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25694_rabbit-spg7-mab-hl2236-azide-and-bsa-free-212024941595.jpg)
![Immunohistochemistry-Paraffin: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] - SPG7 antibody [HL2236] detects SPG7 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. SPG7 stained by SPG7 antibody [HL2236] (NBP3-25694) diluted at 1:20. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25694_rabbit-spg7-mab-hl2236-azide-and-bsa-free-212024941493.jpg)
![Western Blot: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SPG7 antibody [HL2236] (NBP3-25694) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25694_rabbit-spg7-mab-hl2236-azide-and-bsa-free-2120249422671.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SPG7 antibody [HL2236] (NBP3-25694) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25694_rabbit-spg7-mab-hl2236-azide-and-bsa-free-212024943554.jpg)
![Western Blot: GATE-16/GABARAPL2 Antibody [NBP2-82090] - Analysis of GATE-16/GABARAPL2 in human brain tissue lysate with GATE-16/GABARAPL2 antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/GATE-16-GABARAPL2-Antibody-Western-Blot-NBP2-82090-img0001.jpg "Western Blot: GATE-16/GABARAPL2 Antibody [NBP2-82090] - Analysis of GATE-16/GABARAPL2 in human brain tissue lysate with GATE-16/GABARAPL2 antibody at (A) 1 and (B) 2 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: GATE-16/GABARAPL2 Antibody - BSA Free [NBP2-82090] - Immunofluorescence of GATE-16/GABARAPL2 in rat brain tissue with GATE-16/GABARAPL2 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-82090_rabbit-polyclonal-gate-16-gabarapl2-antibody-271120242092042.jpg)
![Immunocytochemistry/ Immunofluorescence: GATE-16/GABARAPL2 Antibody - BSA Free [NBP2-82090] - Immunofluorescence of GATE-16/GABARAPL2 in mouse brain tissue with GATE-16/GABARAPL2 Antibody at 20 ug/mL.Green: GATE-16/GABARAPL2 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-82090_rabbit-polyclonal-gate-16-gabarapl2-antibody-2711202420175113.gif)
![Immunohistochemistry: GATE-16/GABARAPL2 Antibody - BSA Free [NBP2-82090] - Immunohistochemistry of GATE-16/GABARAPL2 in rat brain tissue with GATE-16/GABARAPL2 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-82090_rabbit-polyclonal-gate-16-gabarapl2-antibody-271120241958411.jpg)
![Immunohistochemistry: GATE-16/GABARAPL2 Antibody - BSA Free [NBP2-82090] - Immunohistochemistry of GATE-16/GABARAPL2 in mouse brain tissue with GATE-16/GABARAPL2 Antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-82090_rabbit-polyclonal-gate-16-gabarapl2-antibody-271120241928845.gif)
![Western Blot: TMPRSS2 Antibody (7B9W3) [NBP3-00492] - Analysis of extracts of various cell lines, using TMPRSS2 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat AntiRabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL. Exposure time: 3min.](http://images.novusbio.com/fullsize/TMPRSS2-Antibody-7B9W3-Western-Blot-NBP3-00492-img0001.jpg "Western Blot: TMPRSS2 Antibody (7B9W3) [NBP3-00492] - Analysis of extracts of various cell lines, using TMPRSS2 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat AntiRabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: TMPRSS2 Antibody (7B9W3) [NBP3-00492] - Human esophageal using TMPRSS2 Rabbit mAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TMPRSS2-Antibody-7B9W3-Immunohistochemistry-Paraffin-NBP3-00492-img0002.jpg)
![Western Blot: BCS1L Antibody [NBP2-92916] - Analysis of extracts of various cell lines, using BCS1L at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/BCS1L-Antibody-Western-Blot-NBP2-92916-img0004.jpg "Western Blot: BCS1L Antibody [NBP2-92916] - Analysis of extracts of various cell lines, using BCS1L at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: BCS1L Antibody [NBP2-92916] - Analysis of U2OS cells using BCS1L at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/BCS1L-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92916-img0001.jpg)
![Immunohistochemistry-Paraffin: BCS1L Antibody [NBP2-92916] - Paraffin-embedded rat brain using BCS1L .](http://images.novusbio.com/fullsize/BCS1L-Antibody-Immunohistochemistry-Paraffin-NBP2-92916-img0003.jpg)
![Immunohistochemistry-Paraffin: BCS1L Antibody [NBP2-92916] - Paraffin-embedded human stomach using BCS1L .](http://images.novusbio.com/fullsize/BCS1L-Antibody-Immunohistochemistry-Paraffin-NBP2-92916-img0002.jpg)
![Western Blot: SNAP25 Antibody (JE45-86) [NBP3-32961] - Western blot analysis of SNAP25 on different lysates with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Neuro-2a cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (negative) (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32961) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-288202412493458.jpg "Western Blot: SNAP25 Antibody (JE45-86) [NBP3-32961] - Western blot analysis of SNAP25 on different lysates with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Neuro-2a cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (negative) (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32961) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SNAP25 Antibody (JE45-86) [NBP3-32961] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32961) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-298202414161869.jpg)
![Immunocytochemistry/ Immunofluorescence: SNAP25 Antibody (JE45-86) [NBP3-32961] - Immunocytochemistry analysis of SH-SY5Y cells labeling SNAP25 with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-29202410484855.jpg)
![Flow Cytometry: SNAP25 Antibody (JE45-86) [NBP3-32961] - Flow cytometric analysis of SH-SY5Y cells labeling SNAP25. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32961, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-29202416372172.jpg)
![Western Blot: SNAP23 Antibody (2F5-3D4) [H00008773-M01] - Analysis of SNAP23 expression in transfected 293T cell line by SNAP23 monoclonal antibody (M01), clone 2F5-3D4.Lane 1: SNAP23 transfected lysate (Predicted MW: 23.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SNAP23-Antibody-2F5-3D4-Western-Blot-H00008773-M01-img0009.jpg "Western Blot: SNAP23 Antibody (2F5-3D4) [H00008773-M01] - Analysis of SNAP23 expression in transfected 293T cell line by SNAP23 monoclonal antibody (M01), clone 2F5-3D4.Lane 1: SNAP23 transfected lysate (Predicted MW: 23.4 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: SNAP23 Antibody (2F5-3D4) [H00008773-M01] - Analysis of monoclonal antibody to SNAP23 on formalin-fixed paraffin-embedded human spleen. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/SNAP23-Antibody-2F5-3D4-Immunohistochemistry-Paraffin-H00008773-M01-img0006.jpg)
![Western Blot: SNAP23 Antibody (2F5-3D4) [H00008773-M01] - SNAP23 expression in Raw 264.7.](http://images.novusbio.com/fullsize/SNAP23-Antibody-2F5-3D4-Western-Blot-H00008773-M01-img0001.jpg)
![Immunoprecipitation: SNAP23 Antibody (2F5-3D4) [H00008773-M01] - Analysis of SNAP23 transfected lysate using anti-SNAP23 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SNAP23 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/SNAP23-Antibody-2F5-3D4-Immunoprecipitation-H00008773-M01-img0007.jpg)
![Sandwich ELISA: SNAP23 Antibody (2F5-3D4) [H00008773-M01] - Detection limit for recombinant GST tagged SNAP23 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SNAP23-Antibody-2F5-3D4-Sandwich-ELISA-H00008773-M01-img0008.jpg)
![Western Blot: SNAP23 Antibody [NBP1-83162] - Analysis in human cell line HepG2.](http://images.novusbio.com/fullsize/SNAP23-Antibody-Western-Blot-NBP1-83162-img0006.jpg "Western Blot: SNAP23 Antibody [NBP1-83162] - Analysis in human cell line HepG2.")
![Immunocytochemistry/Immunofluorescence: SNAP23 Antibody [NBP1-83162] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.](http://images.novusbio.com/fullsize/SNAP23-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83162-img0008.jpg)
![Immunohistochemistry-Paraffin: SNAP23 Antibody [NBP1-83162] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/SNAP23-Antibody-Immunohistochemistry-Paraffin-NBP1-83162-img0007.jpg)
![Western Blot: Peripherin Antibody (7C5) [NB300-220] - Analysis of tissue and cell lysates using Peripherin antibody, dilution 1:1,000 (Green): [1] protein standard, [2] rat spinal cord, [3] rat sciatic nerve, [4] mouse spinal cord, [5] pig spinal cord, [6] cow spinal cord, [7] SH-SY5Y cells, and [8] PC12 cells. The band at ~57kDa corresponds to the peripherin protein detected only in rat and mouse lysates, since the antibody is rodent specific.](http://images.novusbio.com/fullsize/Peripherin-Antibody-7C5-Western-Blot-NB300-220-img0004.jpg "Western Blot: Peripherin Antibody (7C5) [NB300-220] - Analysis of tissue and cell lysates using Peripherin antibody, dilution 1:1,000 (Green): [1] protein standard, [2] rat spinal cord, [3] rat sciatic nerve, [4] mouse spinal cord, [5] pig spinal cord, [6] cow spinal cord, [7] SH-SY5Y cells, and [8] PC12 cells. The band at ~57kDa corresponds to the peripherin protein detected only in rat and mouse lysates, since the antibody is rodent specific.")
![Immunocytochemistry/Immunofluorescence: Peripherin Antibody (7C5) [NB300-220] - Analysis of mixed fibroblast and rat pheochromocytoma PC12 cell culture stained with peripherin antibody, dilution 1:500 (Red), and costained with chicken pAb to vimentin, dilution 1:10,000 (Green). Hoechst staining of nuclear DNA (Blue). The peripherin antibody reveals cytoplasmic filamentous staining in the small PC12 cells, while vimentin antibody stains intermediate filaments in the surrounding fibroblastic cells which do not express peripherin.](http://images.novusbio.com/fullsize/Peripherin-Antibody-7C5-Immunocytochemistry-Immunofluorescence-NB300-220-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Peripherin Antibody (7C5) [NB300-220] - Mixed neuron/glia cultures from newborn rat brain stained with NB300-220 antibody to peripherin (green) and rabbit polyclonal antibody to NF-L NBP1-05218 (red channel). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not peripherin. Interestingly, the periperin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both peripherin and NF-L, and so appears golden in this kind of image. The blue channel reveals the localization of DNA.](http://images.novusbio.com/fullsize/Peripherin-Antibody-7C5-Immunocytochemistry-Immunofluorescence-NB300-220-img0003.jpg)
![Western Blot: Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free [NBP3-25705] - Drosophila tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1626] (NBP3-25705) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25705_rabbit-synaptotagmin-1-mab-hl1626-azide-and-bsa-free-212024941483.jpg "Western Blot: Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free [NBP3-25705] - Drosophila tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1626] (NBP3-25705) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free [NBP3-25705] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1626] (NBP3-25705) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25705_rabbit-synaptotagmin-1-mab-hl1626-azide-and-bsa-free-21202494082.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free [NBP3-25705] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1626] (NBP3-25705) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25705_rabbit-synaptotagmin-1-mab-hl1626-azide-and-bsa-free-2120249383198.jpg)
![Western Blot: Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free [NBP3-25705] - Human tissue extract (5 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1626] (NBP3-25705) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25705_rabbit-synaptotagmin-1-mab-hl1626-azide-and-bsa-free-2120249415927.jpg)
![Synaptotagmin 1 antibody [HL1626] detects Synaptotagmin 1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat eye.Synaptotagmin 1 stained by Synaptotagmin 1 antibody [HL1626] (NBP3-25705) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25705_rabbit-synaptotagmin-1-mab-hl1626-azide-and-bsa-free-immunohistochemistry-paraffin-1411202518381714.jpg)