| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62555-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80942 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73200 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43954 | |||
| BSA Free | NBP2-34495-25ul | |||
![ELISA: Mouse HMGB1/HMG-1 ELISA Kit (Colorimetric) [NBP2-62767] - Samples were spiked with high concentrations of Mouse HMGB1/HMG-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-62767_mouse-hmgb1-hmg-1-elisa-kit-colorimetric-132202416173468.jpg "ELISA: Mouse HMGB1/HMG-1 ELISA Kit (Colorimetric) [NBP2-62767] - Samples were spiked with high concentrations of Mouse HMGB1/HMG-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse HMGB1/HMG-1 ELISA Kit (Colorimetric) [NBP2-62767] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-62767_mouse-hmgb1-hmg-1-elisa-kit-colorimetric-4320249115619.jpg)
![Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-20534] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Western-Blot-NBP2-20534-img0015.jpg "Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-20534] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody [NBP2-20534] - SOD1 antibody detects SOD1 protein at cytosol on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SOD1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Immunohistochemistry-Paraffin-NBP2-20534-img0014.jpg)
![Western Blot: Superoxide Dismutase 1 Antibody [NBP2-20534] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 15% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Superoxide-Dismutase-1-Antibody-Western-Blot-NBP2-20534-img0002.jpg)
![Western Blot: Superoxide Dismutase 1 Antibody [NBP2-20534] - Sample (50 ug of whole cell lysate) A: Rat brain, 15% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Superoxide-Dismutase-1-Antibody-Western-Blot-NBP2-20534-img0003.jpg)
![Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-20534] - SOD1 antibody detects SOD1 protein by western blot analysis. Whole cell extracts (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody at a dilution of 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Western-Blot-NBP2-20534-img0013.jpg)
![Immunohistochemistry-Frozen: SOD1/Cu-Zn SOD Antibody [NBP2-20534] - Immunofluorescence photomicrographs of paraffin-embedded mouse fetal brain. Green: SOD1 antibody diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488). Red: beta Tubulin 3/ TUJ1 antibody diluted at 1:100. The signal was developed using goat anti-mouse IgG antibody (Dylight594). Blue: Nuclear staining with Hoechst 33342. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Immunohistochemistry-Frozen-NBP2-20534-img0010.jpg)
![ELISA: SOD1/Cu-Zn SOD Antibody [NBP2-20534] - ELISA detection of SOD1 using SOD1 Antibody for detection at a concentration of 1.5 ug/mL.](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-ELISA-NBP2-20534-img0012.jpg)
![Immunohistochemistry-Paraffin: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP2-16756] - U87 xenograft, using GSTP1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-pi-1-GSTP1-Antibody-Immunohistochemistry-Paraffin-NBP2-16756-img0014.jpg)
![GSTP1 antibody detects GSTP1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GSTP1 protein stained by GSTP1 antibody (NBP2-16756) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-16756_rabbit-polyclonal-glutathione-s-transferase-pi-1-gstp1-antibody-immunocytochemistry-immunofluorescence-2611202518283519.jpg)
![Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Western-Blot-NBP2-62555-img0003.jpg "Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.")
![Flow Cytometry: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Flow-cytometry using the anti-p53 antibody PAb421. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of PAb421 (NBP2-62555, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Flow-Cytometry-NBP2-62555-img0002.jpg)
![Immunocytochemistry /Immunofluorescence: p53 Antibody (PAb421) - Chimeric - Azide and BSA Free [NBP2-62555] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton and stained with NBP2-62555 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62555, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62555_rabbit-monoclonal-p53-antibody-pab421-chimeric-7420252226522.png)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Lack of HIF-P4H-1 increases the amount of p53. Western blot analysis of p53 and MDM2/HDM2 in wt MEFs treated with 10 uM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). Anti-beta-actin (NB600-501 1:20 000) Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-017-17376-0) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Western-Blot-NBP2-17247-img0027.jpg "<b>Biological Strategies Validation. </b>Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Lack of HIF-P4H-1 increases the amount of p53. Western blot analysis of p53 and MDM2/HDM2 in wt MEFs treated with 10 uM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). Anti-beta-actin (NB600-501 1:20 000) Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-017-17376-0) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - Human lung cancer. MDM2 stained by MDM2 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Immunohistochemistry-Paraffin-NBP2-17247-img0028.jpg)
![Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - Human lung cancer, using MDM2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Immunohistochemistry-Paraffin-NBP2-17247-img0003.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Lack of HIF-P4H-1 increases the amount of p53. (a) Western blot analysis of p53 in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (b,c) Western blot analysis of p53 in Hif-p4h-1 & scrambled (Scrm) siRNA transfected HEK293 cells (b) & wt MEFs treated with 50 µM FG4497 for 24 h (c). (d) qPCR analysis of p53 mRNA in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (e) Analysis of p53 protein turnover rate. Hif-p4h-1−/− & wt MEFs were treated with 200 μg/ml of cycloheximide for the indicated time points & whole cell lysates were blotted for p53. (f) Western blot analysis of MDM2 in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (g) Western blot analysis of p53 & MDM2 in wt MEFs treated with 10 μM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). (h,i) Western blot analysis of HIF1 alpha in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h (h) & in scrambled & Hif-p4h-1 siRNA transfected HEK293 cells (i). (j) Western blot analysis of ubiquitination of p53 in Hif-p4h-1 & scrambled siRNA transfected HEK293 cells. The cells were transfected with HA-ubiquitin & endogenous p53 was immunoprecipitated followed by Western blotting with anti-HA & anti-P53 antibodies. (k) Western blot analysis of p53 in Hif-p4h-1−/− & wt MEFs were treated with or without 10 μM MG132 for 5 h. Data are presented as representative Western blots & as mean ± s.d., n = at least 3 individual MEF isolates or experiments. *P Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MDM2/HDM2 stained by MDM2/HDM2 antibody (NBP2-17247) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-91020242091472.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2/HDM2 antibody (NBP2-17247) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-91020242010445.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2/HDM2 antibody (NBP2-17247) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-91020242091496.jpg)
![Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - MDM2/HDM2 antibody detects MDM2/HDM2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.MDM2/HDM2 stained by MDM2/HDM2 antibody (NBP2-17247) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-910202420134015.jpg)
![MDM2 antibody immunoprecipitates MDM2 protein in IP experiments.IP samples: Jurkat whole cell extractA. 30 ug Jurkat cell whole cell extractB. Control with 4 ug of preimmune Rabbit IgGC. Immunoprecipitation of MDM2 protein by 4 ug MDM2 antibody (NBP2-17247)7.5 % SDS-PAGEThe immunoprecipitated MDM2 protein was detected by MDM2 antibody (NBP2-17247) diluted at 1:500.[EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-immunoprecipitation-261120251826130.jpg)
![Western Blot: SCP3/SYCP3 Antibody (6F9C5) [NBP2-23487] - Analysis of human testis tissue (A), human ovary tissue (B), mouse testis tissue (C), and mouse ovary tissue (D) using SCP3 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-6F9C5-Western-Blot-NBP2-23487-img0013.jpg "Western Blot: SCP3/SYCP3 Antibody (6F9C5) [NBP2-23487] - Analysis of human testis tissue (A), human ovary tissue (B), mouse testis tissue (C), and mouse ovary tissue (D) using SCP3 antibody at 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody (6F9C5) [NBP2-23487] - Analysis of HepG2 cells using SCP3 mouse mAb (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labelled with Alexa Fluor-555 phalloidin (red).](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-6F9C5-Immunocytochemistry-Immunofluorescence-NBP2-23487-img0010.jpg)
![Immunohistochemistry: SCP3/SYCP3 Antibody (6F9C5) [NBP2-23487] - Analysis of paraffin-embedded kidney tissues using SYCP3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-6F9C5-Immunohistochemistry-NBP2-23487-img0012.jpg)
![Flow Cytometry: SCP3/SYCP3 Antibody (6F9C5) [NBP2-23487] - Analysis of Jurkat cells using SCP3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-6F9C5-Flow-Cytometry-NBP2-23487-img0009.jpg)
![Immunohistochemistry: SCP3/SYCP3 Antibody (6F9C5) [NBP2-23487] - Analysis of paraffin-emebedded human cervical cancer tissues with DAB staining.](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-6F9C5-Immunohistochemistry-NBP2-23487-img0011.jpg)
![Immunohistochemistry-Paraffin: Arrestin 3 Antibody [NBP1-37003] - Staining (2.5ug/ml) of paraffin embedded Human Retina. Heat induced antigen retrieval with citrate buffer pH 6, HRP-Staining.](http://images.novusbio.com/fullsize/Arrestin-3-Antibody-Immunohistochemistry-Paraffin-NBP1-37003-img0004.jpg "Immunohistochemistry-Paraffin: Arrestin 3 Antibody [NBP1-37003] - Staining (2.5ug/ml) of paraffin embedded Human Retina. Heat induced antigen retrieval with citrate buffer pH 6, HRP-Staining.")
![Immunohistochemistry-Paraffin: Arrestin 3 Antibody [NBP1-37003] - Negative Control showing staining of paraffin embedded Human Retina, with no primary antibody.](http://images.novusbio.com/fullsize/Arrestin-3-Antibody-Immunohistochemistry-Paraffin-NBP1-37003-img0002.jpg)
![Western Blot: p53 Antibody (5E2) [NBP2-00723] - Analysis of extracts (35ug) from 9 different cell lines by using anti-p53 monoclonal antibody.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Western-Blot-NBP2-00723-img0018.jpg "Western Blot: p53 Antibody (5E2) [NBP2-00723] - Analysis of extracts (35ug) from 9 different cell lines by using anti-p53 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: p53 Antibody (5E2) [NBP2-00723] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY p53.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Immunocytochemistry-Immunofluorescence-NBP2-00723-img0013.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (5E2) [NBP2-00723] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-p53 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Immunohistochemistry-Paraffin-NBP2-00723-img0016.jpg)
![Flow Cytometry: p53 Antibody (5E2) [NBP2-00723] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-p53 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Flow-Cytometry-NBP2-00723-img0012.jpg)
![Western Blot: p53 Antibody (5E2) [NBP2-00723] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY p53 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-p53.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Western-Blot-NBP2-00723-img0017.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (5E2) [NBP2-00723] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-p53 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Immunohistochemistry-Paraffin-NBP2-00723-img0014.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (5E2) [NBP2-00723] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-p53 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Immunohistochemistry-Paraffin-NBP2-00723-img0015.jpg)
![Flow Cytometry: p53 Antibody (5E2) [NBP2-00723] - Analysis of Hela cells, using anti-p53 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Flow-Cytometry-NBP2-00723-img0011.jpg)
![Simple Western: p53 Antibody (5E2) [NBP2-00723] - Simple Western lane view shows a specific band for p53 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Simple-Western-NBP2-00723-img0010.jpg)
![Western Blot: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Analysis of Glutathione Synthetase on different lysates using anti-Glutathione Synthetase antibody at 1/500 dilution.Positive control:Lane 1: SiHa Lane 2: Mouse colonLane 3: Rat liver](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-JB95-33-Western-Blot-NBP2-75513-img0008.jpg "Western Blot: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Analysis of Glutathione Synthetase on different lysates using anti-Glutathione Synthetase antibody at 1/500 dilution.Positive control:Lane 1: SiHa Lane 2: Mouse colonLane 3: Rat liver")
![Immunocytochemistry/Immunofluorescence: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Staining Glutathione Synthetase in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-JB95-33-Immunocytochemistry-Immunofluorescence-NBP2-75513-img0002.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Analysis of paraffin-embedded rat epididymis tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-JB95-33-Immunohistochemistry-Paraffin-NBP2-75513-img0007.jpg)
![Flow Cytometry: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Analysis of HepG2 cells with Glutathione Synthetase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-JB95-33-Flow-Cytometry-NBP2-75513-img0001.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Analysis of paraffin-embedded human tonsil tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-JB95-33-Immunohistochemistry-Paraffin-NBP2-75513-img0003.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Analysis of paraffin-embedded human colon cancer tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-JB95-33-Immunohistochemistry-Paraffin-NBP2-75513-img0004.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Analysis of paraffin-embedded human kidney tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-JB95-33-Immunohistochemistry-Paraffin-NBP2-75513-img0005.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Analysis of paraffin-embedded mouse colon tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-JB95-33-Immunohistochemistry-Paraffin-NBP2-75513-img0006.jpg)
![Western Blot: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of BRG1 on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Western-Blot-NBP2-67720-img0013.jpg "Western Blot: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of BRG1 on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Brg1 Antibody (SN20-03) [NBP2-67720] - Staining BRG1 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunocytochemistry-Immunofluorescence-NBP2-67720-img0010.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded mouse epididymis tissue with Rabbit anti-Brg1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0019.jpg)
![Western Blot: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of BRG1 on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Western-Blot-NBP2-67720-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Brg1 Antibody (SN20-03) [NBP2-67720] - Staining BRG1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunocytochemistry-Immunofluorescence-NBP2-67720-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Brg1 Antibody (SN20-03) [NBP2-67720] - Staining BRG1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunocytochemistry-Immunofluorescence-NBP2-67720-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Brg1 Antibody (SN20-03) [NBP2-67720] - Staining BRG1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunocytochemistry-Immunofluorescence-NBP2-67720-img0009.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-BRG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0003.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded human kidney tissue using anti-BRG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0004.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded human tonsil tissue using anti-BRG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0005.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded mouse colon tissue using anti-BRG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0006.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded mouse testis tissue using anti-BRG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0007.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Brg1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0012.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Brg1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0014.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-Brg1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0015.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Brg1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0016.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Brg1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0017.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded mouse kidney tissue using anti-Brg1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 2 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0018.jpg)
![Western Blot: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Analysis of BRG1 on different lysates using anti-BRG1 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: HepG2](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-BSA-Free-Western-Blot-NBP2-74964-img0001.jpg "Western Blot: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Analysis of BRG1 on different lysates using anti-BRG1 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: HepG2")
![Immunocytochemistry/Immunofluorescence: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Staining BRG1 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74964-img0005.jpg)
![Immunohistochemistry: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Analysis of paraffin-embedded mouse colon tissue using anti-BRG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-BSA-Free-Immunohistochemistry-NBP2-74964-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Staining BRG1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74964-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Staining BRG1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74964-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Staining BRG1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74964-img0004.jpg)
![Immunohistochemistry: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-BRG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-BSA-Free-Immunohistochemistry-NBP2-74964-img0006.jpg)
![Immunohistochemistry: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Analysis of paraffin-embedded human kidney tissue using anti-BRG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-BSA-Free-Immunohistochemistry-NBP2-74964-img0007.jpg)
![Immunohistochemistry: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Analysis of paraffin-embedded human tonsil tissue using anti-BRG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-BSA-Free-Immunohistochemistry-NBP2-74964-img0008.jpg)
![Immunohistochemistry: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Analysis of paraffin-embedded mouse testis tissue using anti-BRG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-BSA-Free-Immunohistochemistry-NBP2-74964-img0010.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining in human skin and skeletal muscle tissues. Corresponding p53 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0021.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining in human skin and skeletal muscle tissues. Corresponding p53 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: p53 Antibody (CL2199) [NBP2-34495] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-P53 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Western-Blot-NBP2-34495-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining in U2OS cell line with Anti-p53 monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunocytochemistry-Immunofluorescence-NBP2-34495-img0014.jpg)
![Western Blot: p53 Antibody (CL2199) [NBP2-34495] - Lane 1: Marker [kDa] Lane 2: Human cell line U-251](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Western-Blot-NBP2-34495-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining of human cell line A431 showing cell cycle dependent nuclear (without nucleoli)Staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunocytochemistry-Immunofluorescence-NBP2-34495-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining in MCF7 cell line with Anti-p53 monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunocytochemistry-Immunofluorescence-NBP2-34495-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining in U251 cell line with Anti-p53 monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunocytochemistry-Immunofluorescence-NBP2-34495-img0013.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human colorectal cancer shows strong nuclear positivity in tumor cells, but not in normal mucosa.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0017.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human lung cancer (squamous cell carcinoma) shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0018.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human skin shows moderate to strong nuclear positivity in a subset of epidermal cells.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0019.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0020.jpg)
![Simple Western: p53 Antibody (CL2199) [NBP2-34495] - Simple Western lane view shows a specific band for P53 in 0.2 mg/ml of Hek293T lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Simple-Western-NBP2-34495-img0009.jpg)
![Simple Western: p53 Antibody (CL2199) [NBP2-34495] - Electropherogram image(s) of corresponding Simple Western lane view. p53 antibody was used at 1:50 dilution on Hek293T lysate(s).](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Simple-Western-NBP2-34495-img0011.jpg)