| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61881-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52471-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-1156-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-169SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90437-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98964-50ul | |||
![Western Blot: EphA7 Antibody (6C8G7) [NBP1-47425] - Analysis using EphA7 mouse mAb against truncated GST-EphA7 recombinant protein (1) and truncated EphA7 (aa25-556)-hIgGFc transfected CHOK1 cell lysate (2).](http://images.novusbio.com/fullsize/EphA7-Antibody-6C8G7-Western-Blot-NBP1-47425-img0005.jpg "Western Blot: EphA7 Antibody (6C8G7) [NBP1-47425] - Analysis using EphA7 mouse mAb against truncated GST-EphA7 recombinant protein (1) and truncated EphA7 (aa25-556)-hIgGFc transfected CHOK1 cell lysate (2).")
![Immunohistochemistry-Paraffin: EphA7 Antibody (6C8G7) [NBP1-47425] - Analysis of human Breast tissues using EPHA7 mouse mAb](http://images.novusbio.com/fullsize/EphA7-Antibody-6C8G7-Immunohistochemistry-Paraffin-NBP1-47425-img0004.jpg)
![Western Blot: APBA2 Antibody (1C12A4) [NBP2-61881] - Analysis using APBA2 mAb against human APBA2 (AA: 15-158) recombinant protein. (Expected MW is 42 kDa)](http://images.novusbio.com/fullsize/APBA2-Antibody-1C12A4-Western-Blot-NBP2-61881-img0003.jpg "Western Blot: APBA2 Antibody (1C12A4) [NBP2-61881] - Analysis using APBA2 mAb against human APBA2 (AA: 15-158) recombinant protein. (Expected MW is 42 kDa)")
![Immunocytochemistry/Immunofluorescence: APBA2 Antibody (1C12A4) [NBP2-61881] - Analysis of Hela cells using APBA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/APBA2-Antibody-1C12A4-Immunocytochemistry-Immunofluorescence-NBP2-61881-img0002.jpg)
![Western Blot: APBA2 Antibody (1C12A4) [NBP2-61881] - Analysis using APBA2 mAb against HEK293 (1) and APBA2 (AA: 15-158)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/APBA2-Antibody-1C12A4-Western-Blot-NBP2-61881-img0004.jpg)
![ELISA: APBA2 Antibody (1C12A4) [NBP2-61881] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/APBA2-Antibody-1C12A4-ELISA-NBP2-61881-img0001.jpg)
![Western Blot: GAD2/GAD65 Antibody (5D9G12) [NBP2-52471] - Analysis using GAD2 mAb against HEK293 (1) and GAD2 (AA: 1-100)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-5D9G12-Western-Blot-NBP2-52471-img0003.jpg "Western Blot: GAD2/GAD65 Antibody (5D9G12) [NBP2-52471] - Analysis using GAD2 mAb against HEK293 (1) and GAD2 (AA: 1-100)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: GAD2/GAD65 Antibody (5D9G12) [NBP2-52471] - Analysis of Hela cells using GAD2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-5D9G12-Flow-Cytometry-NBP2-52471-img0001.jpg)
![Western Blot: GAD2/GAD65 Antibody (5D9G12) [NBP2-52471] - Analysis using GAD2 mAb against human GAD2 (AA: 1-100) recombinant protein. (Expected MW is 36.3 kDa)](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-5D9G12-Western-Blot-NBP2-52471-img0002.jpg)
![ELISA: GAD2/GAD65 Antibody (5D9G12) [NBP2-52471] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-5D9G12-ELISA-NBP2-52471-img0004.jpg)
![In vitro assay: Recombinant Mouse alpha-Synuclein Active, Monomer, (Type 1) Protein [NBP2-61595] - Active alpha synuclein aggregate seeds the formation of new alpha Synuclein aggregates from the pool of active monomers. Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate is combined with 100 uM of active alpha Synuclein monomer, as compared to active alpha Synuclein aggregate and active alpha Synuclein monomer alone. Thioflavin T excitation maximum = 450 nm; emission maximum = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Monomer-Type-1-Protein-In-vitro-assay-NBP2-61595-img0004.jpg "In vitro assay: Recombinant Mouse alpha-Synuclein Active, Monomer, (Type 1) Protein [NBP2-61595] - Active alpha synuclein aggregate seeds the formation of new alpha Synuclein aggregates from the pool of active monomers. Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate is combined with 100 uM of active alpha Synuclein monomer, as compared to active alpha Synuclein aggregate and active alpha Synuclein monomer alone. Thioflavin T excitation maximum = 450 nm; emission maximum = 485 nm.")
![Western Blot: NPFFR2 Antibody [NB300-169] - Analysis of human NPFF2 in brain lysate using this antibody at a dilution of 1:500](http://images.novusbio.com/fullsize/NPFFR2-Antibody-Western-Blot-NB300-169-img0001.jpg "Western Blot: NPFFR2 Antibody [NB300-169] - Analysis of human NPFF2 in brain lysate using this antibody at a dilution of 1:500")
![Western Blot: Slit2 Antibody (JE31-87) [NBP3-32955] - Western blot analysis of Slit2 on different lysates with Rabbit anti-Slit2 antibody (NBP3-32955) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (15 ug/Lane) Lane 2: 293T cell lysate (15 ug/Lane) Lane 3: PC-3M cell lysate (15 ug/Lane) Lane 4: Raji cell lysate (15 ug/Lane) Lane 5: HCT 116 cell lysate (15 ug/Lane) Lane 6: HeLa cell lysate (15 ug/Lane) Lane 7: Rat pancreas tissue lysate (30 ug/Lane) Predicted band size: 170 kDa Observed band size: 50/200 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32955) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32955_rabbit-slit2-mab-je31-87-288202412461662.jpg "Western Blot: Slit2 Antibody (JE31-87) [NBP3-32955] - Western blot analysis of Slit2 on different lysates with Rabbit anti-Slit2 antibody (NBP3-32955) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (15 ug/Lane) Lane 2: 293T cell lysate (15 ug/Lane) Lane 3: PC-3M cell lysate (15 ug/Lane) Lane 4: Raji cell lysate (15 ug/Lane) Lane 5: HCT 116 cell lysate (15 ug/Lane) Lane 6: HeLa cell lysate (15 ug/Lane) Lane 7: Rat pancreas tissue lysate (30 ug/Lane) Predicted band size: 170 kDa Observed band size: 50/200 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32955) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Slit2 Antibody (JE31-87) [NBP3-32955] - Immunocytochemistry analysis of A549 cells labeling Slit2 with Rabbit anti-Slit2 antibody (NBP3-32955) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Slit2 antibody (NBP3-32955) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32955_rabbit-slit2-mab-je31-87-29202410484851.jpg)
![Flow Cytometry: Slit2 Antibody (JE31-87) [NBP3-32955] - Flow cytometric analysis of A549 cells labeling Slit2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32955, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32955_rabbit-slit2-mab-je31-87-29202416372174.jpg)
![Immunohistochemistry-Paraffin: SR-AI/MSR Antibody (004) [NBP2-90437] - Immunochemical staining of mouse SR-AI/MSR in mouse liver with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/SR-AI-MSR-Antibody-004-Immunohistochemistry-Paraffin-NBP2-90437-img0003.jpg "Immunohistochemistry-Paraffin: SR-AI/MSR Antibody (004) [NBP2-90437] - Immunochemical staining of mouse SR-AI/MSR in mouse liver with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).")
![Flow Cytometry: SR-AI/MSR Antibody (004) [NBP2-90437] - Flow cytometric analysis of Mouse SR-AI/MSR expression on Raw264.7 cells. Cells were stained with purified anti-Mouse SR-AI/MSR, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/SR-AI-MSR-Antibody-004-Flow-Cytometry-NBP2-90437-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: SR-AI/MSR Antibody (004) [NBP2-90437] - Confocal immunofluorescence analysis of Mouse SR-AI/MSR1 in RAW264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-90437 antibody (1:100) at 4C overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.](http://images.novusbio.com/fullsize/nbp2-90437_rabbit-monoclonal-sr-ai-msr-antibody-004-235202511444470.png)
![Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody (11D5-R) [NBP3-32411] - Western blot analysis of Glutathione S-Transferase pi 1/GSTP1 on different lysates with Mouse anti-Glutathione S-Transferase pi 1/GSTP1 antibody (NBP3-32411) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: HT-29 cell lysate Lane 5: Jurkat cell lysate Lane 6: K-562 cell lysate Lane 7: JAR cell lysate Lane 8: Human brain tissue lysate Lane 9: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32411) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32411_mouse-glutathione-s-transferase-pi-1-gstp1-mab-11d5-r-278202416331264.jpg "Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody (11D5-R) [NBP3-32411] - Western blot analysis of Glutathione S-Transferase pi 1/GSTP1 on different lysates with Mouse anti-Glutathione S-Transferase pi 1/GSTP1 antibody (NBP3-32411) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: HT-29 cell lysate Lane 5: Jurkat cell lysate Lane 6: K-562 cell lysate Lane 7: JAR cell lysate Lane 8: Human brain tissue lysate Lane 9: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32411) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Glutathione S-Transferase pi 1/GSTP1 Antibody (11D5-R) [NBP3-32411] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-Glutathione S-Transferase pi 1/GSTP1 antibody (NBP3-32411) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32411) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32411_mouse-glutathione-s-transferase-pi-1-gstp1-mab-11d5-r-29820241435285.jpg)
![Immunocytochemistry/ Immunofluorescence: Glutathione S-Transferase pi 1/GSTP1 Antibody (11D5-R) [NBP3-32411] - Immunocytochemistry analysis of HT-29 cells labeling Glutathione S-Transferase pi 1/GSTP1 with Mouse anti-Glutathione S-Transferase pi 1/GSTP1 antibody (NBP3-32411) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Glutathione S-Transferase pi 1/GSTP1 antibody (NBP3-32411) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32411_mouse-glutathione-s-transferase-pi-1-gstp1-mab-11d5-r-2920241192326.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32688) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-2882024142262.jpg "Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32688) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32688) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-29820241426269.jpg)
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Immunocytochemistry analysis of HeLa cells labeling p16INK4a/CDKN2A with Mouse anti-p16INK4a/CDKN2AINK4a/CDKN2A antibody (NBP3-32688) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-29202410334055.jpg)
![Western Blot: Glutamate Receptor 4 Antibody (JE36-81) [NBP3-32384] - Western blot analysis of Glutamate Receptor 4 on different lysates with Rabbit anti-Glutamate Receptor 4 antibody (NBP3-32384) at 1/1,000 dilution. Lane 1: Mouse cerebellum tissue lysate (RIPA lysis) Lane 2: Mouse cerebellum tissue lysate (hot lysis) Lane 3: Rat cerebellum tissue lysate (RIPA lysis) Lane 4: Rat cerebellum tissue lysate (hot lysis) Lysates/proteins at 40 ug/Lane. Predicted band size: 101 kDa Observed band size: 110/60 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32384) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32384_rabbit-glutamate-receptor-4-mab-je36-81-278202415242621.jpg "Western Blot: Glutamate Receptor 4 Antibody (JE36-81) [NBP3-32384] - Western blot analysis of Glutamate Receptor 4 on different lysates with Rabbit anti-Glutamate Receptor 4 antibody (NBP3-32384) at 1/1,000 dilution. Lane 1: Mouse cerebellum tissue lysate (RIPA lysis) Lane 2: Mouse cerebellum tissue lysate (hot lysis) Lane 3: Rat cerebellum tissue lysate (RIPA lysis) Lane 4: Rat cerebellum tissue lysate (hot lysis) Lysates/proteins at 40 ug/Lane. Predicted band size: 101 kDa Observed band size: 110/60 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32384) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: Glutamate Receptor 4 Antibody (JE36-81) [NBP3-32384] - Flow cytometric analysis of SH-SY5Y cells labeling Glutamate Receptor 4. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32384, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32384_rabbit-glutamate-receptor-4-mab-je36-81-392024884649.jpg)
![Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-98964] - Immunochemical staining of human SIN3A in human rectum with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/SIN3A-Antibody-Immunohistochemistry-Paraffin-NBP2-98964-img0004.jpg "Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-98964] - Immunochemical staining of human SIN3A in human rectum with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-98964] - Immunochemical staining of human SIN3A in human duodenum with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/SIN3A-Antibody-Immunohistochemistry-Paraffin-NBP2-98964-img0002.jpg)
![Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-98964] - Immunochemical staining of human SIN3A in human ovarian cancer with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/SIN3A-Antibody-Immunohistochemistry-Paraffin-NBP2-98964-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SIN3A Antibody [NBP2-98964] - Immunofluorescence staining of SIN3A in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98964 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus .](http://images.novusbio.com/fullsize/nbp2-98964_rabbit-polyclonal-sin3a-antibody-235202511545674.png)