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![Western Blot: DAT1 [p Thr53] Antibody [NBP2-29533] - Western blot of rat caudate lysate showing specific immunolabeling of the ~55k glycosylated form of the DAT protein phosphorylated at Thr53. Immunolabeling is blocked by the phospho-peptide used as antigen (peptide), but not by the corresponding dephospho-peptide (not shown).](http://images.novusbio.com/fullsize/DAT1-[p-Thr53]-Antibody-Western-Blot-NBP2-29533-img0001.jpg "Western Blot: DAT1 [p Thr53] Antibody [NBP2-29533] - Western blot of rat caudate lysate showing specific immunolabeling of the ~55k glycosylated form of the DAT protein phosphorylated at Thr53. Immunolabeling is blocked by the phospho-peptide used as antigen (peptide), but not by the corresponding dephospho-peptide (not shown).")
![Western Blot: Connexin 43/GJA1 [p Ser368] Antibody [NB100-722] - rat hippocampal lysate showing specific immunolabeling of the ~43k connexin43 phosphorylated at Ser368 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the phospho-Ser368 connexin43 antibody. The immunolabeling of connexin43 is completely eliminated by treatment.](http://images.novusbio.com/fullsize/Connexin-43-GJA1-[p-Ser368]-Antibody-Western-Blot-NB100-722-img0001.jpg "Western Blot: Connexin 43/GJA1 [p Ser368] Antibody [NB100-722] - rat hippocampal lysate showing specific immunolabeling of the ~43k connexin43 phosphorylated at Ser368 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the phospho-Ser368 connexin43 antibody. The immunolabeling of connexin43 is completely eliminated by treatment.")
![Western Blot: Serpin F1/PEDF Antibody (OTI1F7) - Azide and BSA Free [NBP2-74119] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PEDF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PEDF.](http://images.novusbio.com/fullsize/Serpin-F1-PEDF-Antibody-OTI1F7-Azide-and-BSA-Free-Western-Blot-NBP2-74119-img0001.jpg "Western Blot: Serpin F1/PEDF Antibody (OTI1F7) - Azide and BSA Free [NBP2-74119] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PEDF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PEDF.")
![ELISA: Human, Mouse SMN ELISA Kit (Colorimetric) [NBP2-62175] - A typical standard curve for SMN ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.](http://images.novusbio.com/fullsize/Human-Mouse-SMN-ELISA-Kit-Colorimetric-ELISA-NBP2-62175-img0002.jpg "ELISA: Human, Mouse SMN ELISA Kit (Colorimetric) [NBP2-62175] - A typical standard curve for SMN ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.")
![ELISA: Human, Mouse SMN ELISA Kit (Colorimetric) [NBP2-62175] - A parallelism experiment was carried out to determine if the recombinant human SMN standard accurately determines SMN concentrations in biological matrices. To assess parallelism, values for human PBMC lysate and mouse tissue extract was obtained from a s](http://images.novusbio.com/fullsize/Human-Mouse-SMN-ELISA-Kit-Colorimetric-ELISA-NBP2-62175-img0001.jpg)
![Western Blot: PEDF Antibody [NB600-1493] - Y79 cell lysate was probed with 1.0 ug/ml of Anti-PEDF.](http://images.novusbio.com/fullsize/PEDF-Antibody-Western-Blot-NB600-1493-img0001.jpg "Western Blot: PEDF Antibody [NB600-1493] - Y79 cell lysate was probed with 1.0 ug/ml of Anti-PEDF.")
![Western Blot: eNOS [p Ser116] Antibody [NBP2-29664] - Untreated (lane 1) and lambda phosphatase-treated (lane 2) Fetal bovine heart endothelial cell lysate was probed with anti-phospho-eNOS (1:1000).](http://images.novusbio.com/fullsize/eNOS-[p-Ser116]-Antibody-Western-Blot-NBP2-29664-img0002.jpg "Western Blot: eNOS [p Ser116] Antibody [NBP2-29664] - Untreated (lane 1) and lambda phosphatase-treated (lane 2) Fetal bovine heart endothelial cell lysate was probed with anti-phospho-eNOS (1:1000).")
![Immunohistochemistry-Paraffin: EDNRA/Endothelin R Type A Antibody [NLS4069] - Analysis of anti-Endothelin A Receptor antibody with human heart.](http://images.novusbio.com/fullsize/EDNRA-Endothelin-R-Type-A-Antibody-Immunohistochemistry-Paraffin-NLS4069-img0001.jpg "Immunohistochemistry-Paraffin: EDNRA/Endothelin R Type A Antibody [NLS4069] - Analysis of anti-Endothelin A Receptor antibody with human heart.")
![Western Blot: Ceruloplasmin Antibody (JE59-01) [NBP3-32182] - Western blot analysis of Ceruloplasmin on different lysates with Rabbit anti-Ceruloplasmin antibody (NBP3-32182) at 1/1,000 dilution. Lane 1: Rat liver tissue lysate Lane 2: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 122 kDa Observed band size: 140 kDa Exposure time: 3 minutes 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32182) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32182_rabbit-ceruloplasmin-mab-je59-01-278202415212439.jpg "Western Blot: Ceruloplasmin Antibody (JE59-01) [NBP3-32182] - Western blot analysis of Ceruloplasmin on different lysates with Rabbit anti-Ceruloplasmin antibody (NBP3-32182) at 1/1,000 dilution. Lane 1: Rat liver tissue lysate Lane 2: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 122 kDa Observed band size: 140 kDa Exposure time: 3 minutes 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32182) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Western Blot: KvBeta1 Antibody [NBP2-86698] - WB Suggested Anti-KCNAB1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human kidney](http://images.novusbio.com/fullsize/KvBeta1-Antibody-Western-Blot-NBP2-86698-img0001.jpg "Western Blot: KvBeta1 Antibody [NBP2-86698] - WB Suggested Anti-KCNAB1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human kidney")
![Western Blot: Dynamin Antibody (A1B1-R) [NBP3-32282] - Western blot analysis of Dynamin on different lysates with Mouse anti-Dynamin antibody (NBP3-32282) at 1/1,000 dilution. Lane 1: Rat brain tissue lysate Lane 2: Mouse brain tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 97 kDa Observed band size: 100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32282) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32282_mouse-dynamin-mab-a1b1-r-278202416285218.jpg "Western Blot: Dynamin Antibody (A1B1-R) [NBP3-32282] - Western blot analysis of Dynamin on different lysates with Mouse anti-Dynamin antibody (NBP3-32282) at 1/1,000 dilution. Lane 1: Rat brain tissue lysate Lane 2: Mouse brain tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 97 kDa Observed band size: 100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32282) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Abeta 40 Antibody (PSH02-82) [NBP3-32077] - Immunohistochemical analysis of paraffin-embedded mouse model of Alzheimer's brain tissue with Rabbit anti-Abeta 40 antibody (NBP3-32077) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32077) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32077_rabbit-abeta-40-mab-psh02-82-29820241435226.jpg "Immunohistochemistry: Abeta 40 Antibody (PSH02-82) [NBP3-32077] - Immunohistochemical analysis of paraffin-embedded mouse model of Alzheimer's brain tissue with Rabbit anti-Abeta 40 antibody (NBP3-32077) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32077) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: Melanocortin-1 R/MC1R Antibody (JE39-49) [NBP3-32574] - Western blot analysis of Melanocortin-1 R/MC1R on different lysates with Rabbit anti-Melanocortin-1 R/MC1R antibody (NBP3-32574) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (15 ug/Lane) Lane 2: Raji cell lysate (15 ug/Lane) Lane 3: SW620 cell lysate (15 ug/Lane) Lane 4: Rat skin tissue lysate (30 ug/Lane) Predicted band size: 35 kDa Observed band size: 35 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32574) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32574_rabbit-melanocortin-1-r-mc1r-mab-je39-49-278202415242695.jpg "Western Blot: Melanocortin-1 R/MC1R Antibody (JE39-49) [NBP3-32574] - Western blot analysis of Melanocortin-1 R/MC1R on different lysates with Rabbit anti-Melanocortin-1 R/MC1R antibody (NBP3-32574) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (15 ug/Lane) Lane 2: Raji cell lysate (15 ug/Lane) Lane 3: SW620 cell lysate (15 ug/Lane) Lane 4: Rat skin tissue lysate (30 ug/Lane) Predicted band size: 35 kDa Observed band size: 35 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32574) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")