| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90022-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89306-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99542-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98717-50ul | |||
![Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (145) [NBP2-90022] - Lane A: THP1 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDaObserved band size:55 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-145-Western-Blot-NBP2-90022-img0002.jpg "Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (145) [NBP2-90022] - Lane A: THP1 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDaObserved band size:55 kDa(We are unsure as to the identity of these extra bands.)")
![Immunoprecipitation: Tryptophan Hydroxylase 1/TPH-1 Antibody (145) [NBP2-90022] - Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg THP-1 Whole Cell Lysate0.5 uL anti-TPH1 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-TPH1 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 55 kDaObserved band size: 60 kDa](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-145-Immunoprecipitation-NBP2-90022-img0001.jpg)
![Western Blot: Tau [p Ser199] Antibody (JE66-80) [NBP3-32802] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32802) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse brain treated with pp for 1 hour tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32802) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32802_rabbit-tau-p-ser199-mab-je66-80-288202412442962.jpg "Western Blot: Tau [p Ser199] Antibody (JE66-80) [NBP3-32802] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32802) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse brain treated with pp for 1 hour tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32802) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: PRAS40 [p Thr246] Antibody (JE53-94) [NBP3-32790] - Western blot analysis of PRAS40 on different lysates with Rabbit anti-PRAS40 antibody (NBP3-32790) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa serum starved and treated with 100nM insulin for 5 minutes cell lysate Lane 3: HeLa serum starved and treated with 100nM insulin for 5 minutes, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32790) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32790_rabbit-pras40-p-thr246-mab-je53-94-288202412442948.jpg "Western Blot: PRAS40 [p Thr246] Antibody (JE53-94) [NBP3-32790] - Western blot analysis of PRAS40 on different lysates with Rabbit anti-PRAS40 antibody (NBP3-32790) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa serum starved and treated with 100nM insulin for 5 minutes cell lysate Lane 3: HeLa serum starved and treated with 100nM insulin for 5 minutes, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32790) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: CaM Kinase II [p Thr286] Antibody (JE51-81) [NBP3-32752] - Western blot analysis of CaM Kinase II on different lysates with Rabbit anti-CaM Kinase II antibody (NBP3-32752) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Mouse brain treated with pp for 1 hour tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 54~73 kDa Observed band size: 50~60 kDa Exposure time: 53 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32752) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32752_rabbit-cam-kinase-ii-p-thr286-mab-je51-81-288202412461682.jpg "Western Blot: CaM Kinase II [p Thr286] Antibody (JE51-81) [NBP3-32752] - Western blot analysis of CaM Kinase II on different lysates with Rabbit anti-CaM Kinase II antibody (NBP3-32752) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Mouse brain treated with pp for 1 hour tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 54~73 kDa Observed band size: 50~60 kDa Exposure time: 53 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32752) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: IGF-I R/IGF1R Antibody (012) [NBP2-89306] - Staining of Human IGF1R in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human IGF1R monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane.](http://images.novusbio.com/fullsize/IGF-I-R-IGF1R-Antibody-012-Immunocytochemistry-Immunofluorescence-NBP2-89306-img0001.jpg "Immunocytochemistry/Immunofluorescence: IGF-I R/IGF1R Antibody (012) [NBP2-89306] - Staining of Human IGF1R in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human IGF1R monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane.")
![Flow Cytometry: IGF-I R/IGF1R Antibody (012) [NBP2-89306] - Analysis of human IGF1R expression on HeLa cells. Cells were stained with purified anti-Human IGF1R, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/IGF-I-R-IGF1R-Antibody-012-Flow-Cytometry-NBP2-89306-img0002.jpg)
![Western Blot: Caveolin-2 Antibody [NBP2-99542] - Anti-Caveolin-2 rabbit polyclonal antibody at 1:1000 dilution. Lane A: Mouse lung tissue lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 24 kDa (We are unsure as to the identity of these extra bands).x](http://images.novusbio.com/fullsize/Caveolin-2-Antibody-Western-Blot-NBP2-99542-img0004.jpg "Western Blot: Caveolin-2 Antibody [NBP2-99542] - Anti-Caveolin-2 rabbit polyclonal antibody at 1:1000 dilution. Lane A: Mouse lung tissue lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 24 kDa (We are unsure as to the identity of these extra bands).x")
![Immunoprecipitation: Caveolin-2 Antibody [NBP2-99542] - Caveolin-2 was immunoprecipitated using: Lane A: 0.5 mg Mouse lung tissue Lysate 4 ul anti-Caveolin-2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-Caveolin-2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 22 kDa](http://images.novusbio.com/fullsize/Caveolin-2-Antibody-Immunoprecipitation-NBP2-99542-img0003.jpg)
![Western Blot: NEDD4 Antibody (JE35-58) [NBP3-32641] - Western blot analysis of NEDD4 on different lysates with Rabbit anti-NEDD4 antibody (NBP3-32641) at 1/2,000 dilution. Lane 1: U87MG cell lysate Lane 2: A549 cell lysate Lane 3: NIH/3T3 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 149 kDa Observed band size: 110 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32641) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32641_rabbit-nedd4-mab-je35-58-278202416331277.jpg "Western Blot: NEDD4 Antibody (JE35-58) [NBP3-32641] - Western blot analysis of NEDD4 on different lysates with Rabbit anti-NEDD4 antibody (NBP3-32641) at 1/2,000 dilution. Lane 1: U87MG cell lysate Lane 2: A549 cell lysate Lane 3: NIH/3T3 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 149 kDa Observed band size: 110 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32641) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Tau [p Ser324] Antibody (JE30-73) [NBP3-32806] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32806) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 79 kDa Observed band size: 35-130 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32806) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32806_rabbit-tau-p-ser324-mab-je30-73-288202412461657.jpg "Western Blot: Tau [p Ser324] Antibody (JE30-73) [NBP3-32806] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32806) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 79 kDa Observed band size: 35-130 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32806) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: 5-HT7 Antibody [NBP3-14527] - Immunohistochemistry of formalin-fixed, paraffin-embedded human cerebellum after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/5-HT7-Antibody-Immunohistochemistry-Paraffin-NBP3-14527-img0001.jpg "Immunohistochemistry-Paraffin: 5-HT7 Antibody [NBP3-14527] - Immunohistochemistry of formalin-fixed, paraffin-embedded human cerebellum after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: 5-HT7 Antibody [NBP3-14527] - Immunohistochemistry of formalin-fixed, paraffin-embedded human brain, glioblastoma, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/5-HT7 Antibody-Immunohistochemistry-Paraffin-NBP3-14527-img0002.jpg)
![Western Blot: CRMP2 [p Thr514] Antibody (JE57-25) [NBP3-32758] - Western blot analysis of CRMP2 on different lysates with Rabbit anti-CRMP2 antibody (NBP3-32758) at 1/1,000 dilution. Lane 1: PC-12 cell lysate (20 ug/Lane) Lane 2: Human brain tissue lysate (30 ug/Lane) Lane 3: PC-12 treated pp for 1 hour cell lysate (20 ug/Lane) Predicted band size: 62 kDa Observed band size: 70 kDa Exposure time: 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32758) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32758_rabbit-crmp2-p-thr514-mab-je57-25-288202412442936.jpg "Western Blot: CRMP2 [p Thr514] Antibody (JE57-25) [NBP3-32758] - Western blot analysis of CRMP2 on different lysates with Rabbit anti-CRMP2 antibody (NBP3-32758) at 1/1,000 dilution. Lane 1: PC-12 cell lysate (20 ug/Lane) Lane 2: Human brain tissue lysate (30 ug/Lane) Lane 3: PC-12 treated pp for 1 hour cell lysate (20 ug/Lane) Predicted band size: 62 kDa Observed band size: 70 kDa Exposure time: 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32758) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Numb Antibody [NBP2-98717] - Anti-Numb rabbit polyclonal antibody at 1:500 dilution. Lane A: HEK293 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 70 kDa](http://images.novusbio.com/fullsize/Numb-Antibody-Western-Blot-NBP2-98717-img0002.jpg "Western Blot: Numb Antibody [NBP2-98717] - Anti-Numb rabbit polyclonal antibody at 1:500 dilution. Lane A: HEK293 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 70 kDa")
![Immunoprecipitation: Numb Antibody [NBP2-98717] - Numb was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. 4 ul anti-Numb rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein G. Primary antibody: Anti-Numb rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 95 kDa](http://images.novusbio.com/fullsize/Numb-Antibody-Immunoprecipitation-NBP2-98717-img0001.jpg)
![Western Blot: Kv1.1 Antibody [NBP3-30821] - Western blot analysis using Kv1.1 antibody on rat brain lysate.](http://images.novusbio.com/fullsize/nbp3-30821_rabbit-kv1-1-pab-811202420592136.jpg "Western Blot: Kv1.1 Antibody [NBP3-30821] - Western blot analysis using Kv1.1 antibody on rat brain lysate.")