| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80782-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81768-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70187 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59376-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74862 | |||
![Immunohistochemistry-Paraffin: Chromogranin B Antibody [NBP1-80782] - Analysis in human adrenal gland and liver tissues using NBP1-80782 antibody. Corresponding CHGB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Chromogranin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-80782-img0024.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Chromogranin B Antibody [NBP1-80782] - Analysis in human adrenal gland and liver tissues using NBP1-80782 antibody. Corresponding CHGB RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Chromogranin B Antibody [NBP1-80782] - Staining of human adrenal gland, colon, liver and pancreas using Anti-CHGB antibody NBP1-80782 (A) shows similar protein distribution across tissues to independent antibody NBP1-80781 (B).](http://images.novusbio.com/fullsize/Chromogranin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-80782-img0017.jpg)
![Western Blot: Chromogranin B Antibody [NBP1-80782] - Analysis in human cell line SH-SY5Y.](http://images.novusbio.com/fullsize/Chromogranin-B-Antibody-Western-Blot-NBP1-80782-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: Chromogranin B Antibody [NBP1-80782] - Staining of human cell line SH-SY5Y shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Chromogranin-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80782-img0006.jpg)
![Immunohistochemistry-Paraffin: Chromogranin B Antibody [NBP1-80782] - Staining of human pancreas shows strong cytoplasmic positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/Chromogranin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-80782-img0018.jpg)
![Immunohistochemistry-Paraffin: Chromogranin B Antibody [NBP1-80782] - Staining of human colon shows strong cytoplasmic positivity in enteroendocrine cells.](http://images.novusbio.com/fullsize/Chromogranin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-80782-img0019.jpg)
![Immunohistochemistry-Paraffin: Chromogranin B Antibody [NBP1-80782] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Chromogranin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-80782-img0020.jpg)
![Western Blot: PSMA1 Antibody [NBP1-81768] - Analysis using Anti-PSMA1 antibody NBP1-81768 (A) shows similar pattern to independent antibody NBP2-37908 (B).](http://images.novusbio.com/fullsize/Proteasome-20S-C2-Antibody-Western-Blot-NBP1-81768-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PSMA1 Antibody [NBP1-81768] - Analysis using Anti-PSMA1 antibody NBP1-81768 (A) shows similar pattern to independent antibody NBP2-37908 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PSMA1 Antibody [NBP1-81768] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Proteasome-20S-C2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81768-img0007.jpg)
![Immunohistochemistry-Paraffin: PSMA1 Antibody [NBP1-81768] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Proteasome-20S-C2-Antibody-Immunohistochemistry-Paraffin-NBP1-81768-img0011.jpg)
![Western Blot: PSMA1 Antibody [NBP1-81768] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/Proteasome-20S-C2-Antibody-Western-Blot-NBP1-81768-img0006.jpg)
![Immunohistochemistry-Paraffin: PSMA1 Antibody [NBP1-81768] - Staining of human testis shows distinct cytoplasmic and nuclear positivity in cells in seminiferus ducts and Leydig cells.](http://images.novusbio.com/fullsize/Proteasome-20S-C2-Antibody-Immunohistochemistry-Paraffin-NBP1-81768-img0008.jpg)
![Immunohistochemistry-Paraffin: PSMA1 Antibody [NBP1-81768] - Staining of human cervix, uterine shows moderate to strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Proteasome-20S-C2-Antibody-Immunohistochemistry-Paraffin-NBP1-81768-img0010.jpg)
![Immunohistochemistry-Paraffin: PSMA1 Antibody [NBP1-81768] - Staining of human endometrium shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Proteasome-20S-C2-Antibody-Immunohistochemistry-Paraffin-NBP1-81768-img0012.jpg)
![Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - SERCA2 ATPase antibody [HL2352] detects SERCA2 ATPase protein by immunohistochemical analysis. Sample: Paraffin-embedded dog tissues. SERCA2 ATPase stained by SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25678_rabbit-serca2-atpase-mab-hl2352-azide-and-bsa-free-2120249383165.jpg "Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - SERCA2 ATPase antibody [HL2352] detects SERCA2 ATPase protein by immunohistochemical analysis. Sample: Paraffin-embedded dog tissues. SERCA2 ATPase stained by SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - SERCA2 ATPase antibody [HL2352] detects SERCA2 ATPase protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. SERCA2 ATPase stained by SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25678_rabbit-serca2-atpase-mab-hl2352-azide-and-bsa-free-2120249351313.jpg)
![Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - SERCA2 ATPase antibody [HL2352] detects SERCA2 ATPase protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human hepatocellular carcinoma. SERCA2 ATPase stained by SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25678_rabbit-serca2-atpase-mab-hl2352-azide-and-bsa-free-21202494086.jpg)
![Western Blot: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25678_rabbit-serca2-atpase-mab-hl2352-azide-and-bsa-free-2120249362757.jpg)
![Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - SERCA2 ATPase antibody [HL2352] detects SERCA2 ATPase protein by immunohistochemical analysis. Sample: Paraffin-embedded cat brain. SERCA2 ATPase stained by SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25678_rabbit-serca2-atpase-mab-hl2352-azide-and-bsa-free-2120249351325.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25678_rabbit-serca2-atpase-mab-hl2352-azide-and-bsa-free-212024943287.jpg)
![Western Blot: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25678_rabbit-serca2-atpase-mab-hl2352-azide-and-bsa-free-2120249415919.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - Non-transfected (-) and transfected (+) A549 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25678_rabbit-serca2-atpase-mab-hl2352-azide-and-bsa-free-2120249425435.jpg)
![Western Blot: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25678_rabbit-serca2-atpase-mab-hl2352-azide-and-bsa-free-2120249403899.jpg)
![Western Blot: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25678_rabbit-serca2-atpase-mab-hl2352-azide-and-bsa-free-212024941591.jpg)
![Western Blot: APP Antibody (OTI7G9) [NBP2-02358] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Amyloid Precursor Protein (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Amyloid Precursor Protein.](http://images.novusbio.com/fullsize/Amyloid-Precursor-Protein-Antibody-7G9-Western-Blot-NBP2-02358-img0002.jpg "Western Blot: APP Antibody (OTI7G9) [NBP2-02358] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Amyloid Precursor Protein (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Amyloid Precursor Protein.")
![Immunocytochemistry/Immunofluorescence: APP Antibody (OTI7G9) [NBP2-02358] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Amyloid Precursor Protein.](http://images.novusbio.com/fullsize/APP-Antibody-7G9-Immunocytochemistry-Immunofluorescence-NBP2-02358-img0009.jpg)
![Immunohistochemistry-Paraffin: APP Antibody (OTI7G9) [NBP2-02358] - Staining of paraffin-embedded prostate tissue using anti-Amyloid Precursor Protein mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Amyloid-Precursor-Protein-Antibody-7G9-Immunohistochemistry-Paraffin-NBP2-02358-img0007.jpg)
![Flow Cytometry: APP Antibody (OTI7G9) [NBP2-02358] - HEK293T cells transfected with either pCMV6-ENTRY Amyloid Precursor Protein.(Red) or empty vector control plasmid (Blue) were immunostained with anti-Amyloid Precursor Protein mouse monoclonal, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Amyloid-Precursor-Protein-Antibody-7G9-Flow-Cytometry-NBP2-02358-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: APP Antibody (OTI7G9) [NBP2-02358] - Staining of HepG2 cells using anti-Amyloid Precursor Protein mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Amyloid-Precursor-Protein-Antibody-7G9-Immunocytochemistry-Immunofluorescence-NBP2-02358-img0003.jpg)
![Immunohistochemistry-Paraffin: APP Antibody (OTI7G9) [NBP2-02358] - Staining of paraffin-embedded colon tissue using anti-Amyloid Precursor Protein mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Amyloid-Precursor-Protein-Antibody-7G9-Immunohistochemistry-Paraffin-NBP2-02358-img0004.jpg)
![Immunohistochemistry-Paraffin: APP Antibody (OTI7G9) [NBP2-02358] - Staining of paraffin-embedded Kidney tissue using anti-Amyloid Precursor Protein mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Amyloid-Precursor-Protein-Antibody-7G9-Immunohistochemistry-Paraffin-NBP2-02358-img0005.jpg)
![Immunohistochemistry-Paraffin: APP Antibody (OTI7G9) [NBP2-02358] - Staining of paraffin-embedded liver tissue using anti-Amyloid Precursor Protein mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Amyloid-Precursor-Protein-Antibody-7G9-Immunohistochemistry-Paraffin-NBP2-02358-img0006.jpg)
![Western Blot: ATP7A Antibody (S60-4) [NBP2-59376] - Western Blot analysis of Rat Liver showing detection of ~180 kDa ATP7A protein using Mouse Anti-ATP7A Monoclonal Antibody, Clone S60-4 (NBP2-59376). Lane 1: MW Ladder. Lane 2: Rat Liver . Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse ATP7A Monoclonal Antibody (NBP2-59376) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~180 kDa.](http://images.novusbio.com/fullsize/ATP7A-Antibody-S60-4-Western-Blot-NBP2-59376-img0005.jpg "Western Blot: ATP7A Antibody (S60-4) [NBP2-59376] - Western Blot analysis of Rat Liver showing detection of ~180 kDa ATP7A protein using Mouse Anti-ATP7A Monoclonal Antibody, Clone S60-4 (NBP2-59376). Lane 1: MW Ladder. Lane 2: Rat Liver . Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse ATP7A Monoclonal Antibody (NBP2-59376) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~180 kDa.")
![Immunocytochemistry/Immunofluorescence: ATP7A Antibody (S60-4) [NBP2-59376] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody, Clone S60-4 (NBP2-59376). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody (NBP2-59376) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Copper Transporting ATPase 1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/ATP7A-Antibody-S60-4-Immunocytochemistry-Immunofluorescence-NBP2-59376-img0006.jpg)
![Western Blot: ATP7A Antibody (S60-4) [NBP2-59376] - Analysis of Mouse Lung showing detection of >150 kDa Copper Transporting ATPase 1 protein using clone S60-4. 10 ug of sample was loaded and primary antibody was used at 1:1000. Predicted/Observed Size: ~160 kDa. Other band(s): 250 kDa.](http://images.novusbio.com/fullsize/ATP7A-Antibody-S60-4-Western-Blot-NBP2-59376-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ATP7A Antibody (S60-4) [NBP2-59376] - Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Endoplasmic Reticulum, Cytoplasm, Golgi Apparatus, Trans-Golgi Network Membrane, Cell Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize/ATP7A-Antibody-S60-4-Immunocytochemistry-Immunofluorescence-NBP2-59376-img0002.jpg)
![Western Blot: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of PEN2 on mouse spleen tissue lysate using anti-PEN2 antibody at 1/2,000 dilution.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Western-Blot-NBP2-75632-img0009.jpg "Western Blot: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of PEN2 on mouse spleen tissue lysate using anti-PEN2 antibody at 1/2,000 dilution.")
![Immunocytochemistry/Immunofluorescence: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Staining PEN2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunocytochemistry-Immunofluorescence-NBP2-75632-img0003.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded rat cerebellum tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0008.jpg)
![Flow Cytometry: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of A549 cells with PEN2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Flow-Cytometry-NBP2-75632-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Staining PEN2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunocytochemistry-Immunofluorescence-NBP2-75632-img0002.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded human lung cancer tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0004.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded human kidney tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0005.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded human tonsil tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0006.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded mouse testis tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0007.jpg)
![Western Blot: BICD1 Antibody [NBP1-78735] - Whole cell lysate from Jurkat (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and HeLa (H; 50 mcg) cells. Affinity purified rabbit anti-BICD1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. BICD1 was also immunoprecipitated by rabbit anti-BICD1 antibody NBP1-78734 which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/BICD1-Antibody-Western-Blot-NBP1-78735-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: BICD1 Antibody [NBP1-78735] - Whole cell lysate from Jurkat (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and HeLa (H; 50 mcg) cells. Affinity purified rabbit anti-BICD1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. BICD1 was also immunoprecipitated by rabbit anti-BICD1 antibody NBP1-78734 which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation. </b>Western Blot: BICD1 Antibody [NBP1-78735] - Effect of hypoxia on the interaction between HIF-1 alpha and BICD1. Cells were pretreated with MG132 (1 uM) for 30 min prior to hypoxia treatment for 24 h. Co-immunoprecipitation of HIF-1 alpha with IgG and BICD1 were shown in left panel. Total protein expressions in lysate were shown in right panel. n = 3. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41418-018-0241-1), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BICD1-Antibody-Western-Blot-NBP1-78735-img0004.jpg)
![Western Blot: BICD1 Antibody [NBP1-78735] - Role of BICD1 in UCB-MSCs survival under hypoxia. a, b The UCB-MSCs were transfected with BICD1 or NT siRNA for 24 h prior to hypoxia treatment. a Cells were exposed to various durations of hypoxia (0–72 h). Cell viability of UCB-MSCs was measured by WST-1 cell viability assay. n = 8. b Cells were incubated in normoxia or hypoxia conditions for 72 h. Representative images of experimental groups at 0, 24, 48, & 72 h of normoxia or hypoxia incubation are presented. Red-marked cells in representative images indicate PI-positive cells. n = 4. Scale bars are 50 μm (Magnification, × 200). cBICD1 or NT siRNA-transfected cells were incubated in hypoxia for 24 h. Cleaved caspase-9, cleaved caspase-3, & beta -Actin protein expressions were detected by western blot analysis. n = 4. d Cells were transfected with BICD1 or NT siRNA for 24 h prior to hypoxia treatment for 48 h. The percentages of apoptotic cells were analyzed by Annexin V/PI analysis, measured by flowcytometer. Annexin V-positive cells were considered as apoptotic cells. n = 4. Quantitative data are presented as a mean ± S.E.M. All blot images are representative. *p < 0.05 vs. normoxia control with NT siRNA transfection, #p < 0.05 vs. hypoxia with NT siRNA transfection. e The UCB-MSCs were transfected with pcDNA3.1/BICD1-cEGFP vector, pcDNA3.1/cEGFP vector, HIF1A siRNA or NT siRNA for 24 h prior to hypoxia treatment for 72 h. Cell viability data are presented as a mean ± S.E.M. n = 5. *p < 0.05 vs. normoxia control with pcDNA3.1/cEGFP vector & NT siRNA cotransfection, #p < 0.05 vs. hypoxia control with pcDNA3.1/cEGFP vector & NT siRNA cotransfection, @p < 0.05 vs. hypoxia control with pcDNA3.1/BICD1-cEGFP vector & NT siRNA cotransfection Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30464225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78735_rabbit-polyclonal-bicd1-antibody-310202416171413.jpg)
![Western Blot: BICD1 Antibody [NBP1-78735] - Effect of hypoxia on the interaction between HIF1 alpha & BICD1. a Co-immunoprecipitation of HIF1 alpha , Dynein IC, Importin alpha 3, RanBP2 w/ IgG & BICD1 antibodies shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. b Cells immunostained w/ HIF1 alpha & BICD1-specific antibodies. Scale bars are 8 μm (Magnification, × 1,000). White arrow heads indicate co-localization of HIF1 alpha w/ BICD1. c Interaction between HIF1 alpha & BICD1 (HIF1 alpha /BICD1, red) analyzed by PLA. Scale bars are 8 μm (Magnification, × 1,000). n = 6. *p < 0.05 vs. normoxia control. d–f Cells pretreated w/ MG132 (1 μM) for 30 min prior to hypoxia treatment for 24 h. d Co-immunoprecipitation of HIF1 alpha w/ IgG & BICD1 shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. e Cells immunostained w/ HIF1 alpha & BICD1-specific antibodies. White arrow heads indicate co-localization of HIF1 alpha w/ BICD1 in MG132-pretreated UCB-MSCs. Scale bars are 8 μm (Magnification, × 1,000). n = 5. f HIF1 activity measured by dual luciferase reporter assay. n = 6. *p < 0.05 vs. normoxia control w/ MG132 pretreatment. g, h Cells transfected w/ BICD1 or NT siRNA for 24 h prior to hypoxia treatment for 24 h. g Co-immunoprecipitation of Dynein IC w/ IgG & HIF1 alpha antibodies shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. h Interaction between HIF1 alpha & Dynein IC (HIF1 alpha /Dynein IC, red) analyzed by PLA. Scale bars are 8 μm (Magnification, × 1,000). Quantitative data are presented as a mean ± S.E.M. All blots & immunofluorescence images are representative. *p < 0.05 vs. normoxia control w/ NT siRNA transfection, #p < 0.05 vs. hypoxia w/ NT siRNA transfection Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30464225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78735_rabbit-polyclonal-bicd1-antibody-310202416171451.jpg)
![Western Blot: BICD1 Antibody [NBP1-78735] - Effect of hypoxia on the interaction between HIF1 alpha & BICD1. a Co-immunoprecipitation of HIF1 alpha , Dynein IC, Importin alpha 3, RanBP2 w/ IgG & BICD1 antibodies shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. b Cells immunostained w/ HIF1 alpha & BICD1-specific antibodies. Scale bars are 8 μm (Magnification, × 1,000). White arrow heads indicate co-localization of HIF1 alpha w/ BICD1. c Interaction between HIF1 alpha & BICD1 (HIF1 alpha /BICD1, red) analyzed by PLA. Scale bars are 8 μm (Magnification, × 1,000). n = 6. *p < 0.05 vs. normoxia control. d–f Cells pretreated w/ MG132 (1 μM) for 30 min prior to hypoxia treatment for 24 h. d Co-immunoprecipitation of HIF1 alpha w/ IgG & BICD1 shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. e Cells immunostained w/ HIF1 alpha & BICD1-specific antibodies. White arrow heads indicate co-localization of HIF1 alpha w/ BICD1 in MG132-pretreated UCB-MSCs. Scale bars are 8 μm (Magnification, × 1,000). n = 5. f HIF1 activity measured by dual luciferase reporter assay. n = 6. *p < 0.05 vs. normoxia control w/ MG132 pretreatment. g, h Cells transfected w/ BICD1 or NT siRNA for 24 h prior to hypoxia treatment for 24 h. g Co-immunoprecipitation of Dynein IC w/ IgG & HIF1 alpha antibodies shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. h Interaction between HIF1 alpha & Dynein IC (HIF1 alpha /Dynein IC, red) analyzed by PLA. Scale bars are 8 μm (Magnification, × 1,000). Quantitative data are presented as a mean ± S.E.M. All blots & immunofluorescence images are representative. *p < 0.05 vs. normoxia control w/ NT siRNA transfection, #p < 0.05 vs. hypoxia w/ NT siRNA transfection Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30464225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78735_rabbit-polyclonal-bicd1-antibody-31020241616184.jpg)
![Western Blot: BICD1 Antibody [NBP1-78735] - Involvement of Akt/GSK3 beta pathway in BICD1-mediated HIF1 alpha nuclear translocation. a–e The UCB-MSCs were transfected with GSK3 beta or NT siRNA for 24 h prior to hypoxia treatment for 24 h. a Co-immunoprecipitation of HIF1 alpha & Dynein IC with IgG & BICD1 antibodies were shown in left panel. Total protein expressions in lysate were shown in right panel. n = 3. b Interaction between HIF1 alpha & BICD1 (HIF1 alpha /BICD1, red) was analyzed by PLA assay. Scale bars are 8 μm (Magnification, × 1,000). n = 5. c HIF1 alpha , Lamin A/C, & alpha -Tubulin in cytosolic & nuclear fractionized samples were detected by western blot. n = 4. d Cells were immunostained with HIF1 alpha -specific antibody. Scale bars are 8 μm (Magnification, × 1,000). n = 4. e HIF1 activities in NT or GSK3 beta siRNA-transfected cells were analyzed by dual luciferase reporter assay. n = 6. Quantitative data are presented as a mean ± S.E.M. All blots & immunofluorescence images are representative. *p < 0.05 vs. normoxia control with NT siRNA transfection, #p < 0.05 vs. hypoxia with NT siRNA transfection Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30464225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78735_rabbit-polyclonal-bicd1-antibody-31020241616183.jpg)
![Western Blot: LIN-28A Antibody (717) [NBP2-43672] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with LIN-28A antibody diluted at 1:500.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-717-Western-Blot-NBP2-43672-img0008.jpg "Western Blot: LIN-28A Antibody (717) [NBP2-43672] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with LIN-28A antibody diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: LIN-28A Antibody (717) [NBP2-43672] - Analysis of human embryonic stem cell were fixed in 4% paraformaldehyde for 15 min. Green: LIN28 protein stained by LIN28 antibody diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-717-Immunocytochemistry-Immunofluorescence-NBP2-43672-img0002.jpg)
![Immunohistochemistry: LIN-28A Antibody (717) [NBP2-43672] - Analysis of U87 xenograft. Lin28A antibody dilution: 1:100.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-717-Immunohistochemistry-NBP2-43672-img0007.jpg)
![Flow Cytometry: LIN-28A Antibody (717) [NBP2-43672] - Analysis of Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Lin28A antibody [717] dilution: 1:50](http://images.novusbio.com/fullsize/LIN-28A-Antibody-717-Flow-Cytometry-NBP2-43672-img0001.jpg)
![Western Blot: LIN-28A Antibody (717) [NBP2-43672] - Analysis of 30 ug whole cell lysate. A: Non-transfected HeLa lysates B: LIN28 transfected HeLa lysates 12% SDS PAGE diluted at 1:1000.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-717-Western-Blot-NBP2-43672-img0004.jpg)
![Western Blot: LIN-28A Antibody (717) [NBP2-43672] - Analysis of 30 ug whole cell lysate. A: NT2D1 12% SDS PAGE diluted at 1:1000. .](http://images.novusbio.com/fullsize/LIN-28A-Antibody-717-Western-Blot-NBP2-43672-img0005.jpg)
![Western Blot: LIN-28A Antibody (717) [NBP2-43672] - Analysis of A. 30 ug human ESC whole cell lysate/extract 12 % SDS-PAGE Lin28A antibody [717] dilution: 1:1000](http://images.novusbio.com/fullsize/LIN-28A-Antibody-717-Western-Blot-NBP2-43672-img0006.jpg)
![Western Blot: Abeta 40 Antibody [NBP1-44047] - 1-40(lane 1) and 1-42 (lane 2) ABeta peptides were run on a 16.5% TRIS/tricine gel and transferred to PVDF membrane. The peptide concentration was approximately 0.5ng/lane. The blot was incubated with affinity purified AB40 antibody at 1ug/ml, followed by an oat and rabbit -HRP antibody at 1:40,000 and developed using a standard chemiluminescence method. A single band is present only in lane 1 which contains the ABeta 1-40 peptide and no signal is found in lane 2 which contains the ABeta 1-42 peptide.](http://images.novusbio.com/fullsize/Abeta-40-Antibody-Western-Blot-NBP1-44047-img0002.jpg "Western Blot: Abeta 40 Antibody [NBP1-44047] - 1-40(lane 1) and 1-42 (lane 2) ABeta peptides were run on a 16.5% TRIS/tricine gel and transferred to PVDF membrane. The peptide concentration was approximately 0.5ng/lane. The blot was incubated with affinity purified AB40 antibody at 1ug/ml, followed by an oat and rabbit -HRP antibody at 1:40,000 and developed using a standard chemiluminescence method. A single band is present only in lane 1 which contains the ABeta 1-40 peptide and no signal is found in lane 2 which contains the ABeta 1-42 peptide.")
![GAT-1/SLC6A1 antibody [HL3475] detects GAT-1/SLC6A1 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat eye.Green: GAT-1/SLC6A1 stained by GAT-1/SLC6A1 antibody [HL3475] (NBP3-48818) diluted at 1:250.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48818_rabbit-gat-1-slc6a1-mab-hl3475-immunohistochemistry-paraffin-14112025183027.jpg "GAT-1/SLC6A1 antibody [HL3475] detects GAT-1/SLC6A1 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat eye.Green: GAT-1/SLC6A1 stained by GAT-1/SLC6A1 antibody [HL3475] (NBP3-48818) diluted at 1:250.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![GAT-1/SLC6A1 antibody [HL3475] detects GAT-1/SLC6A1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: GAT-1/SLC6A1 stained by GAT-1/SLC6A1 antibody [HL3475] (NBP3-48818) diluted at 1:250.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48818_rabbit-gat-1-slc6a1-mab-hl3475-immunohistochemistry-paraffin-1411202518415215.jpg)
![Unboiled human tissue extract was separated by 7.5% SDS-PAGE, and the membrane was blotted with GAT-1/SLC6A1 antibody [HL3475] (NBP3-48818) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48818_rabbit-gat-1-slc6a1-mab-hl3475-western-blot-141120251831213.jpg)
![<b>Orthogonal Strategies Validation. </b>GAT-1/SLC6A1 antibody [HL3475] detects GAT-1/SLC6A1 protein by immunohistochemical analysis.Sample: Paraffin-embedded human tissues.GAT-1/SLC6A1 stained by GAT-1/SLC6A1 antibody [HL3475] (NBP3-48818) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 minCorresponding RNA levels (RPKM) in the tissues are based on NCBI database.](http://images.novusbio.com/fullsize/nbp3-48818_rabbit-gat-1-slc6a1-mab-hl3475-immunohistochemistry-paraffin-141120251844815.jpg)
![Unboiled various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAT-1/SLC6A1 antibody [HL3475] (NBP3-48818) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48818_rabbit-gat-1-slc6a1-mab-hl3475-western-blot-1411202518341521.jpg)
![Boiled and unboiled rat tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAT-1/SLC6A1 antibody [HL3475] (NBP3-48818) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48818_rabbit-gat-1-slc6a1-mab-hl3475-western-blot-141120251842522.jpg)
![GAT-1/SLC6A1 antibody [HL3475] detects GAT-1/SLC6A1 protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse eye.Green: GAT-1/SLC6A1 stained by GAT-1/SLC6A1 antibody [HL3475] (NBP3-48818) diluted at 1:250.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25623_rabbit-relb-mab-hl2222-azide-and-bsa-free-immunoprecipitation-14112025185540.jpg)
![Western Blot: VTI1A Antibody (1F4) [NBP2-45413] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/VTI1A-Antibody-1F4-Western-Blot-NBP2-45413-img0006.jpg "Western Blot: VTI1A Antibody (1F4) [NBP2-45413] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry: VTI1A Antibody (1F4) [NBP2-45413] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/VTI1A-Antibody-1F4-Immunohistochemistry-NBP2-45413-img0005.jpg)
![Western Blot: VTI1A Antibody (1F4) [NBP2-45413] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VTI1A.](http://images.novusbio.com/fullsize/VTI1A-Antibody-1F4-Western-Blot-NBP2-45413-img0007.jpg)
![Immunohistochemistry: VTI1A Antibody (1F4) [NBP2-45413] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/VTI1A-Antibody-1F4-Immunohistochemistry-NBP2-45413-img0001.jpg)
![Immunohistochemistry: VTI1A Antibody (1F4) [NBP2-45413] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/VTI1A-Antibody-1F4-Immunohistochemistry-NBP2-45413-img0002.jpg)
![Immunohistochemistry: VTI1A Antibody (1F4) [NBP2-45413] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/VTI1A-Antibody-1F4-Immunohistochemistry-NBP2-45413-img0003.jpg)
![Immunohistochemistry: VTI1A Antibody (1F4) [NBP2-45413] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/VTI1A-Antibody-1F4-Immunohistochemistry-NBP2-45413-img0004.jpg)
![Western Blot: Glycine Receptor Alpha 1 Antibody [NB300-113] - Rat spinal cord lysate (SC) showing specific immunolabeling of the ~48 kDa alpha 1- and alpha 2-subunits of the glycine receptor. Immunolabeling was absent from a rat hippocampal lysate (H), as the glycine receptor is not expressed in the hippocampus.](http://images.novusbio.com/fullsize/Glycine-Receptor-Alpha-1-Antibody-Western-Blot-NB300-113-img0005.jpg "Western Blot: Glycine Receptor Alpha 1 Antibody [NB300-113] - Rat spinal cord lysate (SC) showing specific immunolabeling of the ~48 kDa alpha 1- and alpha 2-subunits of the glycine receptor. Immunolabeling was absent from a rat hippocampal lysate (H), as the glycine receptor is not expressed in the hippocampus.")
![Western Blot: Glycine Receptor Alpha 1 Antibody [NB300-113] - Rat spinal cord showing specific immunolabeling of the ~48k a1- and a2-subunits of the glycine receptor. The labeling was absent from a Rat hippocampal (hipp) lysate as the glycine receptor is not expressed in the hippocampus.](http://images.novusbio.com/fullsize/Glycine-Receptor-Alpha-1-Antibody-Western-Blot-NB300-113-img0003.jpg)
![Western Blot: Glycine Receptor Alpha 1 Antibody [NB300-113] - Western blot of 10 ug of rat spinal cord. The antibody is specific for the ~48 kDa a1- and a2-subunitsof the glycine receptor.](http://images.novusbio.com/fullsize/Glycine-Receptor-Alpha-1-Antibody-Western-Blot-NB300-113-img0004.jpg)