| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-32352-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70687 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33321-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86335-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85438-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70688 | |||
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP2-32352] - Analysis in human placenta and prostate tissues. Corresponding LIN-28B RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP2-32352-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP2-32352] - Analysis in human placenta and prostate tissues. Corresponding LIN-28B RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP2-32352] - Staining of human kidney, liver, placenta and prostate using Anti-LIN-28B antibody NBP2-32352 (A) shows similar protein distribution across tissues to independent antibody NBP1-85438 (B).](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP2-32352-img0015.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: LIN-28B Antibody [NBP2-32352] - Analysis in human cell line HEK 293 and human cell line MCF-7.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Western-Blot-NBP2-32352-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: LIN-28B Antibody [NBP2-32352] - Immunofluorescent staining of human cell line HEK 293 shows localization to nucleus, nucleoli & cytosol.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-32352-img0004.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP2-32352] - Staining of human prostate shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP2-32352-img0019.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP2-32352] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP2-32352-img0016.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP2-32352] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP2-32352-img0017.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP2-32352] - Staining of human placenta shows moderate positivity in endothelial cells.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP2-32352-img0018.jpg)
![Western Blot: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Estrogen Receptor alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Estrogen Receptor alpha.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Western-Blot-NBP2-01371-img0001.jpg "Western Blot: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Estrogen Receptor alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Estrogen Receptor alpha.")
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] Staining of paraffin-embedded Human tonsil using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0009.jpg)
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0002.jpg)
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0003.jpg)
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - Staining of paraffin-embedded Human breast tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0004.jpg)
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - Staining of paraffin-embedded Human Kidney tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0005.jpg)
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - Staining of paraffin-embedded Human lymphoma tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0006.jpg)
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - Staining of paraffin-embedded Human pancreas tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0007.jpg)
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - Staining of paraffin-embedded Human prostate tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0008.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Estrogen Receptor alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with ant](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Western-Blot-NBP2-70687-img0001.jpg "Western Blot: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Estrogen Receptor alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with ant")
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Human prostate tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0008.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0002.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0003.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Human breast tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0004.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Human Kidney tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0005.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Human lymphoma tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0006.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Human pancreas tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0007.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Human tonsil using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0009.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Western Blot Analysis of human MCF-7 cell lysate using ER alpha/NR3A1 Antibody (ER506).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-ER506-Western-Blot-NBP2-33320-img0017.jpg "Western Blot: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Western Blot Analysis of human MCF-7 cell lysate using ER alpha/NR3A1 Antibody (ER506).")
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Formalin-fixed, paraffin-embedded human Endometrium stained with ER alpha/NR3A1 Antibody (ER506).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-ER506-Immunohistochemistry-Paraffin-NBP2-33320-img0015.jpg)
![Flow Cytometry: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Flow Cytometry for human ER-alpha on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled ER alpha/NR3A1 antibody (ER506).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-ER506-Flow-Cytometry-NBP2-33320-img0018.jpg)
![Simple Western: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Simple Western lane view shows a specific band for Estrogen Receptor in 0.2 mg/ml of MCF-7 (left) and T47D (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-ER506-Simple-Western-NBP2-33320-img0007.jpg)
![Simple Western: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Electropherogram image of the corresponding Simple Western lane. Estrogen Receptor antibody was used at 10 ug/ml dilution of MCF-7 and T47D lysates(s) respectively.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-ER506-Simple-Western-NBP2-33320-img0009.jpg)
![Protein Array: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ER alpha/NR3A1 Antibody (ER506). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD?s) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD?s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-ER506-Protein-Array-NBP2-33320-img0016.jpg)
![Western Blot: ATP5A Antibody [NBP2-38525] - Analysis using Anti-ATP5A1 antibody NBP2-38525 (A) shows similar pattern to independent antibody NBP2-38470 (B).](http://images.novusbio.com/fullsize/ATP5A-Antibody-Western-Blot-NBP2-38525-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: ATP5A Antibody [NBP2-38525] - Analysis using Anti-ATP5A1 antibody NBP2-38525 (A) shows similar pattern to independent antibody NBP2-38470 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-38525] - Staining of human kidney.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-38525-img0007.jpg)
![Western Blot: ATP5A Antibody [NBP2-38525] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/ATP5A-Antibody-Western-Blot-NBP2-38525-img0002.jpg)
![Immunohistochemistry: ATP5A Antibody [NBP2-38525] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-NBP2-38525-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-38525] - Staining of human colon, heart muscle, kidney and liver using Anti-ATP5A1 antibody NBP2-38525 (A) shows similar protein distribution across tissues to independent antibody NBP2-38470 (B).](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-38525-img0003.jpg)
![Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-38525] - Staining of human liver.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-38525-img0005.jpg)
![Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-38525] - Staining of human heart muscle.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-38525-img0006.jpg)
![Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-38525] - Staining of human colon.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-38525-img0008.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (SC0536) [NBP2-67565] - Analysis of paraffin-embedded human kidney tissue using anti-Apolipoprotein E antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-SC0536-Immunohistochemistry-Paraffin-NBP2-67565-img0001.jpg "Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (SC0536) [NBP2-67565] - Analysis of paraffin-embedded human kidney tissue using anti-Apolipoprotein E antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (SC0536) [NBP2-67565] - Analysis of paraffin-embedded human liver tissue using anti-Apolipoprotein E antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-SC0536-Immunohistochemistry-Paraffin-NBP2-67565-img0002.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (SC0536) [NBP2-67565] - Analysis of paraffin-embedded human tonsil tissue using anti-Apolipoprotein E antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-SC0536-Immunohistochemistry-Paraffin-NBP2-67565-img0003.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (SC0536) [NBP2-67565] - Mouse brain tissue with Rabbit anti-Apolipoprotein E antibody at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-67565_rabbit-monoclonal-apolipoprotein-e-apoe-antibody-sc0536-immunohistochemistry-paraffin-682024115144..jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (SC0536) [NBP2-67565] - AD mouse brain tissue with Rabbit anti-Apolipoprotein E antibody at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-67565_rabbit-monoclonal-apolipoprotein-e-apoe-antibody-sc0536-immunohistochemistry-paraffin-682024115246..jpg)
![Western Blot: Apolipoprotein E/ApoE Antibody (SC0536) [NBP2-67565] - Analysis of Apolipoprotein E on human plasma tissue lysates with Rabbit anti-Apolipoprotein E antibody at 1/1,000 dilution.Lysates/proteins at 20 ug/Lane.Predicted band size: 36 kDaObserved band size: 33 kDaExposure time: 6 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67565_rabbit-monoclonal-apolipoprotein-e-apoe-antibody-sc0536-western-blot-68202411549..jpg)
![Western Blot: PON1 Antibody (4X7X2) [NBP3-16187] - Western blot analysis of extracts of Mouse liver, using PON1 Rabbit mAb (NBP3-16187) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PON1-Antibody-4X7X2-Western-Blot-NBP3-16187-img0005.jpg "Western Blot: PON1 Antibody (4X7X2) [NBP3-16187] - Western blot analysis of extracts of Mouse liver, using PON1 Rabbit mAb (NBP3-16187) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: PON1 Antibody (4X7X2) [NBP3-16187] - Western blot analysis of extracts of HepG2 cells, using PON1 Rabbit mAb (NBP3-16187) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/PON1-Antibody-4X7X2-Western-Blot-NBP3-16187-img0006.jpg)
![Immunohistochemistry-Paraffin: PON1 Antibody (4X7X2) [NBP3-16187] - Immunohistochemistry of paraffin-embedded human liver using PON1 Rabbit mAb (NBP3-16187) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PON1-Antibody-4X7X2-Immunohistochemistry-Paraffin-NBP3-16187-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: PON1 Antibody (4X7X2) [NBP3-16187] - Confocal imaging of paraffin-embedded Rat liver tissue using PON1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16187_rabbit-pon1-mab-4x7x2-52202511392230.jpg)
![Immunocytochemistry/ Immunofluorescence: PON1 Antibody (4X7X2) [NBP3-16187] - Confocal imaging of paraffin-embedded Mouse liver tissue using PON1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16187_rabbit-pon1-mab-4x7x2-5220251255594.jpg)
![Immunocytochemistry/ Immunofluorescence: PON1 Antibody (4X7X2) [NBP3-16187] - Confocal imaging of paraffin-embedded Human liver tissue using PON1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16187_rabbit-pon1-mab-4x7x2-5220251242555.jpg)
![Western Blot: beta-3 Adrenergic R/ADRB3 Antibody [H00000155-B01P] - Analysis of ADRB3 expression in transfected 293T cell line by ADRB3 polyclonal antibody. Lane 1: ADRB3 transfected lysate(44.88 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/beta-3-Adrenergic-R-ADRB3-Antibody-Western-Blot-H00000155-B01P-img0005.jpg "Western Blot: beta-3 Adrenergic R/ADRB3 Antibody [H00000155-B01P] - Analysis of ADRB3 expression in transfected 293T cell line by ADRB3 polyclonal antibody. Lane 1: ADRB3 transfected lysate(44.88 KDa). Lane 2: Non-transfected lysate.")
![Flow Cytometry: beta-3 Adrenergic R/ADRB3 Antibody [H00000155-B01P] - Analysis of negative control 293 cells (Black) and ADRB3 expressing 293 cells (Green) using ADRB3 purified MaxPab mouse polyclonal antibody.](http://images.novusbio.com/fullsize/beta-3-Adrenergic-R-ADRB3-Antibody-Flow-Cytometry-H00000155-B01P-img0003.jpg)
![Western Blot: Kynurenine 3-Monooxygenase/KMO Antibody [NBP1-86335] - Western blot analysis of function protein expression on primary human renal cortical epithelial cells challenged with cytokines. Primary human renal tubular epithelial cells are challenged with cytokine cocktail (15nM IFN gamma , 6nM TNF alpha , & 3nM IL1 beta ). (A, B) Expression of Snail, E-Cad, TJP1, SMA. AhR, IDO, KMO, KY, MHCI & II. (C, D) Densitometric quantitation of protein in (A, B). All Western blots are representative of at least three independent experiments. *P < 0.05, ***P < 0.0001 versus to cells without treatment, analysis is multiple t-tests, pairwise comparison of individual time-point to control indicated same p-value. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34305900), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-86335_rabbit-polyclonal-kynurenine-3-monooxygenase-kmo-antibody-310202415291989.jpg "Western Blot: Kynurenine 3-Monooxygenase/KMO Antibody [NBP1-86335] - Western blot analysis of function protein expression on primary human renal cortical epithelial cells challenged with cytokines. Primary human renal tubular epithelial cells are challenged with cytokine cocktail (15nM IFN gamma , 6nM TNF alpha , & 3nM IL1 beta ). (A, B) Expression of Snail, E-Cad, TJP1, SMA. AhR, IDO, KMO, KY, MHCI & II. (C, D) Densitometric quantitation of protein in (A, B). All Western blots are representative of at least three independent experiments. *P < 0.05, ***P < 0.0001 versus to cells without treatment, analysis is multiple t-tests, pairwise comparison of individual time-point to control indicated same p-value. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34305900), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-85438-img0012.jpg "Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Staining of human liver shows no positivity in hepatocytes as expected.")
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-85438-img0002.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Staining of human prostate shows low expression as expected.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-85438-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Staining of human kidney, liver, placenta and testis using Anti-LIN28B antibody NBP1-85438 (A) shows similar protein distribution across tissues to independent antibody NBP2-32352 (B).](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-85438-img0005.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Staining of human liver.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-85438-img0006.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Staining of human testis.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-85438-img0007.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Staining of human kidney.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-85438-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Analysis in human placenta and prostate tissues. Corresponding LIN-28B RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-85438-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Staining of human kidney, liver, placenta and prostate using Anti-LIN-28B antibody NBP1-85438 (A) shows similar protein distribution across tissues to independent antibody NBP2-32352 (B).](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-85438-img0010.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-85438-img0011.jpg)
![Western Blot: Estrogen Receptor Antibody (2D3) [NBP2-45522] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ER alpha/NR3A1.](http://images.novusbio.com/fullsize/Estrogen-Receptor-Antibody-2D3-Western-Blot-NBP2-45522-img0006.jpg "Western Blot: Estrogen Receptor Antibody (2D3) [NBP2-45522] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ER alpha/NR3A1.")
![Immunohistochemistry: Estrogen Receptor Antibody (2D3) [NBP2-45522] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/Estrogen-Receptor-Antibody-2D3-Immunohistochemistry-NBP2-45522-img0005.jpg)
![Immunohistochemistry: Estrogen Receptor Antibody (2D3) [NBP2-45522] - Analysis of Human breast tissue.](http://images.novusbio.com/fullsize/Estrogen-Receptor-Antibody-2D3-Immunohistochemistry-NBP2-45522-img0001.jpg)
![Immunohistochemistry: Estrogen Receptor Antibody (2D3) [NBP2-45522] - Analysis of Carcinoma of Human kidney tissue.](http://images.novusbio.com/fullsize/Estrogen-Receptor-Antibody-2D3-Immunohistochemistry-NBP2-45522-img0002.jpg)
![Immunohistochemistry: Estrogen Receptor Antibody (2D3) [NBP2-45522] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/Estrogen-Receptor-Antibody-2D3-Immunohistochemistry-NBP2-45522-img0003.jpg)
![Immunohistochemistry: Estrogen Receptor Antibody (2D3) [NBP2-45522] - Analysis of Human endometrium tissue.](http://images.novusbio.com/fullsize/Estrogen-Receptor-Antibody-2D3-Immunohistochemistry-NBP2-45522-img0004.jpg)