| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59376-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43807 | |||
| BSA Free | NBP2-46645-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43926 | |||
| BSA Free | NBP2-34478-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89768-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70687 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03471-20ul | |||
![Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (7711) diluted at 1:500.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-7711-Western-Blot-NBP2-43826-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (7711) diluted at 1:500.")
![<b>Genetic Strategies Validation. </b>Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 10 % SDS-PAGE Glutamine synthetase antibody [7711] dilution: 1:1000](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-7711-Western-Blot-NBP2-43826-img0001.jpg)
![Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-7711-Western-Blot-NBP2-43826-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Glutamine Synthetase antibody [GT7711] detects Glutamine Synthetase protein in glial cells by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron & glia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Glutamine Synthetase stained by Glutamine Synthetase antibody [GT7711] (NBP2-43826) diluted at 1:500.Red: Tau, a Tau marker, stained by Tau antibody diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-43826_mouse-monoclonal-glutamine-synthetase-antibody-7711-910202419593549.jpg)
![Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine Synthetase antibody [GT7711] (NBP2-43826) diluted at 1:250000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43826_mouse-monoclonal-glutamine-synthetase-antibody-7711-91020242063368.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Glutamine Synthetase antibody [GT7711] detects Glutamine Synthetase protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.Glutamine Synthetase stained by Glutamine Synthetase antibody [GT7711] (NBP2-43826) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43826_mouse-monoclonal-glutamine-synthetase-antibody-7711-91020242041239.jpg)
![Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.Glutamine synthetase stained by Glutamine synthetase antibody [GT7711] (NBP2-43826) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43826_mouse-monoclonal-glutamine-synthetase-antibody-7711-immunohistochemistry-paraffin-26112025199277.jpg)
![Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina.Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [GT7711] (NBP2-43826) diluted at 1:250. Red: PKC alpha protein stained by PKC alpha antibody [GT1876] diluted at 1:250.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-43826_mouse-monoclonal-glutamine-synthetase-antibody-7711-immunohistochemistry-frozen-261120251823624.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT7711] (NBP2-43826) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43826_mouse-monoclonal-glutamine-synthetase-antibody-7711-western-blot-2611202518422920.jpg)
![Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: Glutamine synthetase stained by Glutamine synthetase antibody [GT7711] (NBP2-43826) diluted at 1:200.Red: beta Tubulin 3/ Tuj1 , a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43826_mouse-monoclonal-glutamine-synthetase-antibody-7711-immunohistochemistry-paraffin-261120251844100.jpg)
![Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat stomach.Glutamine synthetase stained by Glutamine synthetase antibody [GT7711] (NBP2-43826) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43826_mouse-monoclonal-glutamine-synthetase-antibody-7711-immunohistochemistry-paraffin-261120251995415.jpg)
![Western Blot: ATP7A Antibody (S60-4) [NBP2-59376] - Western Blot analysis of Rat Liver showing detection of ~180 kDa ATP7A protein using Mouse Anti-ATP7A Monoclonal Antibody, Clone S60-4 (NBP2-59376). Lane 1: MW Ladder. Lane 2: Rat Liver . Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse ATP7A Monoclonal Antibody (NBP2-59376) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~180 kDa.](http://images.novusbio.com/fullsize/ATP7A-Antibody-S60-4-Western-Blot-NBP2-59376-img0005.jpg "Western Blot: ATP7A Antibody (S60-4) [NBP2-59376] - Western Blot analysis of Rat Liver showing detection of ~180 kDa ATP7A protein using Mouse Anti-ATP7A Monoclonal Antibody, Clone S60-4 (NBP2-59376). Lane 1: MW Ladder. Lane 2: Rat Liver . Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse ATP7A Monoclonal Antibody (NBP2-59376) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~180 kDa.")
![Immunocytochemistry/Immunofluorescence: ATP7A Antibody (S60-4) [NBP2-59376] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody, Clone S60-4 (NBP2-59376). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody (NBP2-59376) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Copper Transporting ATPase 1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/ATP7A-Antibody-S60-4-Immunocytochemistry-Immunofluorescence-NBP2-59376-img0006.jpg)
![Western Blot: ATP7A Antibody (S60-4) [NBP2-59376] - Analysis of Mouse Lung showing detection of >150 kDa Copper Transporting ATPase 1 protein using clone S60-4. 10 ug of sample was loaded and primary antibody was used at 1:1000. Predicted/Observed Size: ~160 kDa. Other band(s): 250 kDa.](http://images.novusbio.com/fullsize/ATP7A-Antibody-S60-4-Western-Blot-NBP2-59376-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ATP7A Antibody (S60-4) [NBP2-59376] - Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Endoplasmic Reticulum, Cytoplasm, Golgi Apparatus, Trans-Golgi Network Membrane, Cell Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize/ATP7A-Antibody-S60-4-Immunocytochemistry-Immunofluorescence-NBP2-59376-img0002.jpg)
![Western Blot: Glutamine Synthetase Antibody (3013) [NBP2-46645] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3013-Western-Blot-NBP2-46645-img0009.jpg "Western Blot: Glutamine Synthetase Antibody (3013) [NBP2-46645] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue")
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (3013) [NBP2-46645] - Staining of human kidney shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3013-Immunohistochemistry-Paraffin-NBP2-46645-img0007.jpg)
![Western Blot: Glutamine Synthetase Antibody (3013) [NBP2-46645] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3013-Western-Blot-NBP2-46645-img0008.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (3013) [NBP2-46645] - Staining of rat striatum shows moderate immunoreactivity in a subset of astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3013-Immunohistochemistry-NBP2-46645-img0001.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (3013) [NBP2-46645] - Staining of rat cerebral cortex shows positivity in a subset of astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3013-Immunohistochemistry-NBP2-46645-img0002.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (3013) [NBP2-46645] - Staining of rat cerebral cortex shows positivity in a subset of astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3013-Immunohistochemistry-NBP2-46645-img0004.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (3013) [NBP2-46645] - Staining of mouse hippocampal formation shows positivity in a subset of astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3013-Immunohistochemistry-NBP2-46645-img0005.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (3013) [NBP2-46645] - Staining of human cerebral cortex shows strong immunoreactivity in astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3013-Immunohistochemistry-Paraffin-NBP2-46645-img0003.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (3013) [NBP2-46645] - Staining of human cerebellum shows positivity in glial cells.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3013-Immunohistochemistry-Paraffin-NBP2-46645-img0006.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ESR1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Western-Blot-NBP2-34478-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ESR1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of MCF7 cells using the Anti-ESR1 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Immunocytochemistry-Immunofluorescence-NBP2-34478-img0009.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human testis shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Immunohistochemistry-NBP2-34478-img0005.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human endometrium shows strong nuclear immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Immunohistochemistry-NBP2-34478-img0001.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human cervix shows strong nuclear positivity in epithelial cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Immunohistochemistry-NBP2-34478-img0002.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human fallopian tube shows strong immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Immunohistochemistry-NBP2-34478-img0003.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human breast cancer shows moderate to strong nuclear positivity in a subset of tumor cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Immunohistochemistry-NBP2-34478-img0004.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89768] - Staining of human cerebellum, cerebral cortex, kidney and liver using Anti-GLUL antibody NBP1-89768 (A) shows similar protein distribution across tissues to independent antibody NBP1-89767 (B).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-89768-img0008.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89768] - Staining of human cerebellum, cerebral cortex, kidney and liver using Anti-GLUL antibody NBP1-89768 (A) shows similar protein distribution across tissues to independent antibody NBP1-89767 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89768] - Staining in human cerebral cortex and kidney tissues using NBP1-89768 antibody. Corresponding GLUL RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-89768-img0012.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89768] - Staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-89768-img0009.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89768] - Staining of human kidney shows only very weak cytoplasmic positivity in a subset of cells in tubules.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-89768-img0010.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89768] - Staining of human cerebellum shows strong cytoplasmic positivity in glial cells.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-89768-img0007.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89768] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-89768-img0011.jpg)
![Immunohistochemistry-Paraffin: 5-HT7 Antibody [NBP1-46598] - Sections of rat brain. Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 Aum.](http://images.novusbio.com/fullsize/5-HT7-Antibody-Immunohistochemistry-Paraffin-NBP1-46598-img0002.jpg "Immunohistochemistry-Paraffin: 5-HT7 Antibody [NBP1-46598] - Sections of rat brain. Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 Aum.")
![Immunohistochemistry-Paraffin: 5-HT7 Antibody [NBP1-46598] - Sections of rat brain. Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.](http://images.novusbio.com/fullsize/5-HT7-Antibody-Immunohistochemistry-Paraffin-NBP1-46598-img0001.jpg)
![Western Blot: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Estrogen Receptor alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Estrogen Receptor alpha.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Western-Blot-NBP2-01371-img0001.jpg "Western Blot: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Estrogen Receptor alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Estrogen Receptor alpha.")
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] Staining of paraffin-embedded Human tonsil using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0009.jpg)
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0002.jpg)
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0003.jpg)
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - Staining of paraffin-embedded Human breast tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0004.jpg)
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - Staining of paraffin-embedded Human Kidney tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0005.jpg)
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - Staining of paraffin-embedded Human lymphoma tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0006.jpg)
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - Staining of paraffin-embedded Human pancreas tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0007.jpg)
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - Staining of paraffin-embedded Human prostate tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0008.jpg)
![Western Blot: Glutamine Synthetase Antibody [NBP3-03471] - Western blot analysis of extracts of various cell lines, using Glutamine Synthetase antibody (NBP3-03471) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Glutamine Synthetase Antibody-Western Blot-NBP3-03471-img0008.jpg "Western Blot: Glutamine Synthetase Antibody [NBP3-03471] - Western blot analysis of extracts of various cell lines, using Glutamine Synthetase antibody (NBP3-03471) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP3-03471] - Immunohistochemistry of paraffin-embedded rat liver using [KO Validated] Glutamine Synthetase Rabbit pAb (NBP3-03471) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP3-03471-img0006.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP3-03471] - Immunohistochemistry of paraffin-embedded human esophageal using [KO Validated] Glutamine Synthetase Rabbit pAb (NBP3-03471) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP3-03471-img0004.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP3-03471] - Immunohistochemistry of paraffin-embedded human liver using [KO Validated] Glutamine Synthetase Rabbit pAb (NBP3-03471) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP3-03471-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: Glutamine Synthetase Antibody - BSA Free [Glutamine Synthetase] - Immunofluorescence analysis of NIH/3T3 cells using Glutamine Synthetase Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03471_rabbit-polyclonal-glutamine-synthetase-antibody-5220251628083.jpg)
![Immunocytochemistry/ Immunofluorescence: Glutamine Synthetase Antibody - BSA Free [Glutamine Synthetase] - Immunofluorescence analysis of PC-12 cells using Glutamine Synthetase Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03471_rabbit-polyclonal-glutamine-synthetase-antibody-5220251632149.jpg)
![Immunohistochemistry: Ceruloplasmin Antibody (6C3K9) [Ceruloplasmin] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Ceruloplasmin Rabbit mAb at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15868_rabbit-ceruloplasmin-mab-6c3k9-52202517251917.jpg "Immunohistochemistry: Ceruloplasmin Antibody (6C3K9) [Ceruloplasmin] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Ceruloplasmin Rabbit mAb at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.")
![Immunohistochemistry: Ceruloplasmin Antibody (6C3K9) [Ceruloplasmin] - Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using Ceruloplasmin Rabbit mAb at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15868_rabbit-ceruloplasmin-mab-6c3k9-52202517213574.jpg)
![Immunohistochemistry: Ceruloplasmin Antibody (6C3K9) [Ceruloplasmin] - Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using Ceruloplasmin Rabbit mAb at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15868_rabbit-ceruloplasmin-mab-6c3k9-52202517192998.jpg)
![Immunocytochemistry/ Immunofluorescence: Ceruloplasmin Antibody (6C3K9) [Ceruloplasmin] - Confocal imaging of human liver using Ceruloplasmin Rabbit mAb . DAPI was used for nuclear staining (blue). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.](http://images.novusbio.com/fullsize/nbp3-15868_rabbit-ceruloplasmin-mab-6c3k9-52202517213560.jpg)
![Immunocytochemistry/ Immunofluorescence: Ceruloplasmin Antibody (6C3K9) [Ceruloplasmin] - Immunofluorescence analysis of paraffin-embedded mouse liver using Ceruloplasmin Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15868_rabbit-ceruloplasmin-mab-6c3k9-52202517223997.jpg)
![Immunocytochemistry/ Immunofluorescence: Ceruloplasmin Antibody (6C3K9) [Ceruloplasmin] - Immunofluorescence analysis of paraffin-embedded rat liver using Ceruloplasmin Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15868_rabbit-ceruloplasmin-mab-6c3k9-52202517184551.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Estrogen Receptor alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with ant](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Western-Blot-NBP2-70687-img0001.jpg "Western Blot: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Estrogen Receptor alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with ant")
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Human prostate tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0008.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0002.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0003.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Human breast tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0004.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Human Kidney tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0005.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Human lymphoma tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0006.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Human pancreas tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0007.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Human tonsil using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0009.jpg)
![Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of Tryptophan Hydroxylase 1/TPH1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HL-60 cell lysate Lane 2: Hela cell lysate](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-SC53-07-Western-Blot-NBP2-67580-img0004.jpg "Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of Tryptophan Hydroxylase 1/TPH1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HL-60 cell lysate Lane 2: Hela cell lysate")
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of paraffin-embedded rat brain tissue using anti-Tryptophan Hydroxylase/TPH-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-SC53-07-Immunohistochemistry-Paraffin-NBP2-67580-img0005.jpg)
![Flow Cytometry: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of Hela cells with TPH1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-SC53-07-Flow-Cytometry-NBP2-67580-img0001.jpg)
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of paraffin-embedded mouse cerebellum tissue using anti-TPH1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-SC53-07-Immunohistochemistry-Paraffin-NBP2-67580-img0002.jpg)
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of paraffin-embedded rat brain tissue using anti-TPH1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-SC53-07-Immunohistochemistry-Paraffin-NBP2-67580-img0003.jpg)
![Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of TPH1 on different lysates with Rabbit anti-TPH1 antibody at 1/1,000 dilution.Lane 1: MDA-MB-231-si NT cell lysateLane 2: MDA-MB-231-si TPH1 cell lysateLysates/proteins at 10 ug/Lane.Predicted band size: 51 kDaObserved band size: 72 kDaExposure time: 3 minutes;4-20% SDS-PAGE gel.NBP2-67580 was shown to specifically react with TPH1 in MDA-MB-231-si NT cells. Weakened band was observed when MDA-MB-231-si TPH1 sample was tested. MDA-MB-231-si NT and Hela-si TPH1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1/1,000) and Loading control antibody (Rabbit anti-GAPDH, (1/10,000) were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-rabbit IgG-HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67580_rabbit-monoclonal-tryptophan-hydroxylase-1-tph-1-antibody-sc53-07-western-blot-462024131133..jpg)