| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-15334-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03685-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03610-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59407-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35336-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-16042-20ul | |||
![Western Blot: Fibrillarin Antibody [NBP2-26151] - NBP2-26151 (0.1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Western-Blot-NBP2-26151-img0001.jpg "Western Blot: Fibrillarin Antibody [NBP2-26151] - NBP2-26151 (0.1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/ Immunofluorescence: TBR1 Antibody (1U1K5) [NBP3-15769] - Confocal imaging of paraffin-embedded Mouse brain using TBR1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15769_rabbit-tbr1-mab-1u1k5-82202520984.jpg "Immunocytochemistry/ Immunofluorescence: TBR1 Antibody (1U1K5) [NBP3-15769] - Confocal imaging of paraffin-embedded Mouse brain using TBR1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.")
![Western Blot: Cav3.2 Antibody [NBP3-15334] - Western blot analysis of extracts of various cell lines, using Cav3.2 antibody (NBP3-15334) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Cav3.2-Antibody-Western-Blot-NBP3-15334-img0002.jpg "Western Blot: Cav3.2 Antibody [NBP3-15334] - Western blot analysis of extracts of various cell lines, using Cav3.2 antibody (NBP3-15334) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunocytochemistry/Immunofluorescence: Cav3.2 Antibody [NBP3-15334] - Immunofluorescence analysis of NIH/3T3 cells using Cav3.2 Rabbit pAb (NBP3-15334) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cav3.2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15334-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Cav3.2 Antibody - Azide and BSA Free [Cav3.2] - Immunofluorescence analysis of NIH/3T3 cells using Cav3.2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15334_rabbit-cav3-2-pab-5220251723579.jpg)
![Western Blot: Glycine Receptor alpha 2 Antibody [NBP3-03685] - Analysis of various lysates, using GLRA2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/antibody/nbp3-03685_rabbit-polyclonal-glycine-receptor-alpha-2-antibody-western-blot-1311202493456..png "Western Blot: Glycine Receptor alpha 2 Antibody [NBP3-03685] - Analysis of various lysates, using GLRA2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: Kv7.1 Antibody [NBP3-03610] - Analysis of extracts of various cell lines, using KCNQ1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Kv7.1-Antibody-Western-Blot-NBP3-03610-img0003.jpg "Western Blot: Kv7.1 Antibody [NBP3-03610] - Analysis of extracts of various cell lines, using KCNQ1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 30s.")
![Western Blot: Kv7.1 Antibody [NBP3-03610] - Analysis of extracts of various cell lines, using KCNQ1 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Kv7.1-Antibody-Western-Blot-NBP3-03610-img0002.jpg)
![Western Blot: Park7/DJ-1 Antibody (1E12) [NBP2-59407] - Lane 1: Kidney tissue lysates](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-1E12-Western-Blot-NBP2-59407-img0001.jpg "Western Blot: Park7/DJ-1 Antibody (1E12) [NBP2-59407] - Lane 1: Kidney tissue lysates")
![Immunocytochemistry/Immunofluorescence: Park7/DJ-1 Antibody (1E12) [NBP2-59407] - Analysis of PARK7 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PARK7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of PARK7 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PARK7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-1E12-Immunocytochemistry-Immunofluorescence-NBP2-59407-img0002.jpg)
![Western Blot: Laminin beta 1 Antibody [NBP3-35336] - Western blot analysis of various lysates using Laminin beta 1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35336_rabbit-laminin-beta-1-pab-101220241233876.jpg "Western Blot: Laminin beta 1 Antibody [NBP3-35336] - Western blot analysis of various lysates using Laminin beta 1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")
![Immunocytochemistry/ Immunofluorescence: Laminin beta 1 Antibody [NBP3-35336] - Immunofluorescence analysis of L929 cells using Laminin beta 1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35336_rabbit-laminin-beta-1-pab-1012202411552810.jpg)
![Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (9E5H3) [NBP3-33354] - Western blot analysis of lysates from Rat liver, using Glutathione Peroxidase 1/GPX1 Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-33354_rabbit-glutathione-peroxidase-1-gpx1-mab-9e5h3-912202414201385.jpg "Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (9E5H3) [NBP3-33354] - Western blot analysis of lysates from Rat liver, using Glutathione Peroxidase 1/GPX1 Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (9E5H3) [NBP3-33354] - Western blot analysis of various lysates, using Glutathione Peroxidase 1/GPX1 Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-33354_rabbit-glutathione-peroxidase-1-gpx1-mab-9e5h3-912202415175212.jpg)
![Western Blot: MNX1/HLXB9 Antibody [NBP3-16042] - Western blot analysis of extracts of Rat liver, using MNX1/HLXB9 antibody (NBP3-16042) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/MNX1-HLXB9-Antibody-Western-Blot-NBP3-16042-img0001.jpg "Western Blot: MNX1/HLXB9 Antibody [NBP3-16042] - Western blot analysis of extracts of Rat liver, using MNX1/HLXB9 antibody (NBP3-16042) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Western Blot: MNX1/HLXB9 Antibody [NBP3-16042] - Western blot analysis of extracts of various cell lines, using MNX1/HLXB9 antibody (NBP3-16042) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/MNX1-HLXB9-Antibody-Western-Blot-NBP3-16042-img0002.jpg)
![Western Blot: CD133 Antibody (A8C3-R) [NBP3-32124] - Western blot analysis of CD133 on different lysates with Mouse anti-CD133 antibody (NBP3-32124) at 1/5,000 dilution. Lane 1: HT-29 cell lysate Lane 2: SW480 cell lysate Lane 3: NCCIT cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 97 kDa Observed band size: 120 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32124) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32124_mouse-cd133-mab-a8c3-r-27820241631670.jpg "Western Blot: CD133 Antibody (A8C3-R) [NBP3-32124] - Western blot analysis of CD133 on different lysates with Mouse anti-CD133 antibody (NBP3-32124) at 1/5,000 dilution. Lane 1: HT-29 cell lysate Lane 2: SW480 cell lysate Lane 3: NCCIT cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 97 kDa Observed band size: 120 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32124) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD133 Antibody (A8C3-R) [NBP3-32124] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-CD133 antibody (NBP3-32124) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32124) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32124_mouse-cd133-mab-a8c3-r-298202414161867.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein D Antibody [NBP1-40025] - Analysis of anti-APOD / Apolipoprotein D antibody with human kidney at concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Apolipoprotein-D-Antibody-Immunohistochemistry-Paraffin-NBP1-40025-img0002.jpg "Immunohistochemistry-Paraffin: Apolipoprotein D Antibody [NBP1-40025] - Analysis of anti-APOD / Apolipoprotein D antibody with human kidney at concentration 10 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: OCT4 Antibody (A3-C11-R) [NBP3-31983] - Immunocytochemistry analysis of NCCIT cells labeling OCT4 with Mouse anti-OCT4 antibody (NBP3-31983) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-OCT4 antibody (NBP3-31983) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-31983_mouse-oct4-mab-a3-c11-r-29202410334010.jpg "Immunocytochemistry/ Immunofluorescence: OCT4 Antibody (A3-C11-R) [NBP3-31983] - Immunocytochemistry analysis of NCCIT cells labeling OCT4 with Mouse anti-OCT4 antibody (NBP3-31983) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-OCT4 antibody (NBP3-31983) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.")
![Western Blot: OCT4 Antibody (A3-C11-R) [NBP3-31983] - Western blot analysis of OCT4 on NCCIT cell lysates with Mouse anti-OCT4 antibody (NBP3-31983) at 1/1,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 39 kDa Observed band size: 45 kDa Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-31983) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-31983_mouse-oct4-mab-a3-c11-r-392024845356.jpg)