| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38305-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92867-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37329-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12906-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37428-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50538-0.025ml | |||
![Western Blot: Parvalbumin Antibody (6C7K6) [NBP3-15664] - Analysis of extracts of various cell lines, using Parvalbumin (PVALB) antibody (NBP3-15664) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-6C7K6-Western-Blot-NBP3-15664-img0004.jpg "Western Blot: Parvalbumin Antibody (6C7K6) [NBP3-15664] - Analysis of extracts of various cell lines, using Parvalbumin (PVALB) antibody (NBP3-15664) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: Parvalbumin Antibody (6C7K6) [NBP3-15664] - Mouse brain using Parvalbumin (PVALB) Rabbit mAb (NBP3-15664) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-6C7K6-Immunohistochemistry-Paraffin-NBP3-15664-img0003.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody (6C7K6) [NBP3-15664] - Rat brain using Parvalbumin (PVALB) Rabbit mAb (NBP3-15664) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-6C7K6-Immunohistochemistry-Paraffin-NBP3-15664-img0001.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody (6C7K6) [NBP3-15664] - Human appendix using Parvalbumin (PVALB) Rabbit mAb (NBP3-15664) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-6C7K6-Immunohistochemistry-Paraffin-NBP3-15664-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Parvalbumin Antibody (6C7K6) [Parvalbumin] - Confocal imaging of paraffin-embedded Rat brain tissue using Parvalbumin (PVALB) Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15664_rabbit-parvalbumin-mab-6c7k6-52202517361245.jpg)
![Immunohistochemistry: Parvalbumin Antibody (6C7K6) [Parvalbumin] - Immunohistochemistry analysis of paraffin-embedded Human kidney using Parvalbumin (PVALB) Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15664_rabbit-parvalbumin-mab-6c7k6-5220251734526.jpg)
![Immunocytochemistry/ Immunofluorescence: Parvalbumin Antibody (6C7K6) [Parvalbumin] - Confocal imaging of paraffin-embedded Human kidney tissue using Parvalbumin (PVALB) Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15664_rabbit-parvalbumin-mab-6c7k6-5220251736371.jpg)
![Immunocytochemistry/ Immunofluorescence: Parvalbumin Antibody (6C7K6) [Parvalbumin] - Confocal imaging of paraffin-embedded Mouse brain tissue using Parvalbumin (PVALB) Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15664_rabbit-parvalbumin-mab-6c7k6-5220251733527.jpg)
![Immunocytochemistry/ Immunofluorescence: NFkB p105/p50 Antibody [NBP3-38305] - Immunofluorescence analysis of NIH/3T3 cells using NFkB p105/p50 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38305_rabbit-nfkb-p105-p50-pab-1012202411545558.jpg "Immunocytochemistry/ Immunofluorescence: NFkB p105/p50 Antibody [NBP3-38305] - Immunofluorescence analysis of NIH/3T3 cells using NFkB p105/p50 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunocytochemistry/ Immunofluorescence: NFkB p105/p50 Antibody [NBP3-38305] - Immunofluorescence analysis of A-549 cells using NFkB p105/p50 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38305_rabbit-nfkb-p105-p50-pab-101220241233811.jpg)
![Immunocytochemistry/ Immunofluorescence: NFkB p105/p50 Antibody [NBP3-38305] - Immunofluorescence analysis of U2OS cells using NFkB p105/p50 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38305_rabbit-nfkb-p105-p50-pab-1012202411572633.jpg)
![Immunohistochemistry: NFkB p105/p50 Antibody [NBP3-38305] - Immunohistochemistry analysis of paraffin-embedded Mouse intestin using NFkB p105/p50 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38305_rabbit-nfkb-p105-p50-pab-1012202411531043.jpg)
![Immunohistochemistry: NFkB p105/p50 Antibody [NBP3-38305] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using NFkB p105/p50 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38305_rabbit-nfkb-p105-p50-pab-10122024120610.jpg)
![Immunohistochemistry: NFkB p105/p50 Antibody [NBP3-38305] - Immunohistochemistry analysis of paraffin-embedded Rat lung using NFkB p105/p50 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38305_rabbit-nfkb-p105-p50-pab-101220241233849.jpg)
![Western Blot: NFkB p105/p50 Antibody [NBP3-38305] - Western blot analysis of lysates from Mouse lung, using NFkB p105/p50 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp3-38305_rabbit-nfkb-p105-p50-pab-1012202411572669.jpg)
![Immunohistochemistry-Paraffin: ATPG Antibody [NBP2-92867] - Paraffin-embedded rat ovary using ATPG .](http://images.novusbio.com/fullsize/ATPG-Antibody-Immunohistochemistry-Paraffin-NBP2-92867-img0006.jpg "Immunohistochemistry-Paraffin: ATPG Antibody [NBP2-92867] - Paraffin-embedded rat ovary using ATPG .")
![Immunocytochemistry/Immunofluorescence: ATPG Antibody [NBP2-92867] - Analysis of U-2 OS cells using ATPG . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ATPG-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92867-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ATPG Antibody [NBP2-92867] - Analysis of C6 cells using ATPG . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ATPG-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92867-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ATPG Antibody [NBP2-92867] - Analysis of L929 cells using ATPG . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ATPG-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92867-img0002.jpg)
![Immunohistochemistry-Paraffin: ATPG Antibody [NBP2-92867] - Paraffin-embedded human lung cancer using ATPG .](http://images.novusbio.com/fullsize/ATPG-Antibody-Immunohistochemistry-Paraffin-NBP2-92867-img0004.jpg)
![Immunohistochemistry-Paraffin: ATPG Antibody [NBP2-92867] - Paraffin-embedded human placenta using ATPG .](http://images.novusbio.com/fullsize/ATPG-Antibody-Immunohistochemistry-Paraffin-NBP2-92867-img0005.jpg)
![Western Blot: ATPG Antibody - Azide and BSA Free [NBP2-92867] - Western blot analysis of various lysates, using ATPG Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp2-92867_rabbit-polyclonal-atpg-antibody-42202514251872.jpg)
![Western Blot: ZEB1 Antibody (2A8H3) [NBP2-37329] - Western blot analysis using ZEB1 mAb against HEK293 (1) and ZEB1 (AA: 967-1108)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Western-Blot-NBP2-37329-img0003.jpg "Western Blot: ZEB1 Antibody (2A8H3) [NBP2-37329] - Western blot analysis using ZEB1 mAb against HEK293 (1) and ZEB1 (AA: 967-1108)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (2A8H3) [NBP2-37329] - Immunofluorescence analysis of Hela cells using ZEB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Immunocytochemistry-Immunofluorescence-NBP2-37329-img0004.jpg)
![Immunohistochemistry: ZEB1 Antibody (2A8H3) [NBP2-37329] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ZEB1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Immunohistochemistry-NBP2-37329-img0007.jpg)
![Flow Cytometry: ZEB1 Antibody (2A8H3) [NBP2-37329] - Flow cytometric analysis of Hela cells using ZEB1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Flow-Cytometry-NBP2-37329-img0005.jpg)
![Western Blot: ZEB1 Antibody (2A8H3) [NBP2-37329] - Western blot analysis using ZEB1 mAb against human ZEB1 recombinant protein. (Expected MW is 41.7 kDa)](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Western-Blot-NBP2-37329-img0002.jpg)
![Immunohistochemistry: ZEB1 Antibody (2A8H3) [NBP2-37329] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ZEB1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Immunohistochemistry-NBP2-37329-img0006.jpg)
![ELISA: ZEB1 Antibody (2A8H3) [NBP2-37329] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-ELISA-NBP2-37329-img0001.jpg)
![Western Blot: TRPM7 Antibody (S74) [NBP2-12906] - Western Blot analysis of Human Cell lysates showing detection of TrpM7 protein using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74 (NBP2-12906). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody (NBP2-12906) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/TRPM7-Antibody-S74-Western-Blot-NBP2-12906-img0008.jpg "Western Blot: TRPM7 Antibody (S74) [NBP2-12906] - Western Blot analysis of Human Cell lysates showing detection of TrpM7 protein using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74 (NBP2-12906). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody (NBP2-12906) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: TRPM7 Antibody (S74) [NBP2-12906] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74 (NBP2-12906). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody (NBP2-12906) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpM7 Antibody (D) Composite.](http://images.novusbio.com/fullsize/TRPM7-Antibody-S74-Immunocytochemistry-Immunofluorescence-NBP2-12906-img0011.jpg)
![Immunohistochemistry: TRPM7 Antibody (S74) [NBP2-12906] - Immunohistochemistry analysis using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74 (NBP2-12906). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody (NBP2-12906) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/TRPM7-Antibody-S74-Immunohistochemistry-NBP2-12906-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: TRPM7 Antibody (S74) [NBP2-12906] - Human hippocampal tissues.](http://images.novusbio.com/fullsize/TRPM7-Antibody-S74-Immunocytochemistry-Immunofluorescence-NBP2-12906-img0004.jpg)
![Immunohistochemistry: TRPM7 Antibody (S74) [NBP2-12906] - Immunohistochemistry analysis using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74 (NBP2-12906). Tissue: Brain Slice. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody (NBP2-12906) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT. Localization: Nuclear staining of both neurons and glia.](http://images.novusbio.com/fullsize/TRPM7-Antibody-S74-Immunohistochemistry-NBP2-12906-img0009.jpg)
![Western Blot: CaMKIV Antibody (8C5B8) [NBP2-37428] - Western blot analysis using CAMK4 mouse mAb against Jurkat (1), SK-N-SH (2), Raji (3), and HeLa (4) cell lysate.](http://images.novusbio.com/fullsize/CaMKIV-Antibody-8C5B8-Western-Blot-NBP2-37428-img0004.jpg "Western Blot: CaMKIV Antibody (8C5B8) [NBP2-37428] - Western blot analysis using CAMK4 mouse mAb against Jurkat (1), SK-N-SH (2), Raji (3), and HeLa (4) cell lysate.")
![Immunocytochemistry/Immunofluorescence: CaMKIV Antibody (8C5B8) [NBP2-37428] - Immunofluorescence analysis of HepG2 cells using CAMK4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/CaMKIV-Antibody-8C5B8-Immunocytochemistry-Immunofluorescence-NBP2-37428-img0005.jpg)
![Immunohistochemistry: CaMKIV Antibody (8C5B8) [NBP2-37428] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CAMK4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CaMKIV-Antibody-8C5B8-Immunohistochemistry-NBP2-37428-img0007.jpg)
![Western Blot: CaMKIV Antibody (8C5B8) [NBP2-37428] - Western blot analysis using CAMK4 mAb against human CAMK4 recombinant protein. (Expected MW is 54.8 kDa)](http://images.novusbio.com/fullsize/CaMKIV-Antibody-8C5B8-Western-Blot-NBP2-37428-img0002.jpg)
![Western Blot: CaMKIV Antibody (8C5B8) [NBP2-37428] - Western blot analysis using CAMK4 mAb against HEK293 (1) and CAMK4 (AA: 35-292)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CaMKIV-Antibody-8C5B8-Western-Blot-NBP2-37428-img0003.jpg)
![Immunohistochemistry: CaMKIV Antibody (8C5B8) [NBP2-37428] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using CAMK4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CaMKIV-Antibody-8C5B8-Immunohistochemistry-NBP2-37428-img0006.jpg)
![ELISA: CaMKIV Antibody (8C5B8) [NBP2-37428] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CaMKIV-Antibody-8C5B8-ELISA-NBP2-37428-img0001.jpg)
![Immunohistochemistry: Kv3.1 [p Ser503] Antibody [NB300-279] - IHC staining of medial nucleus of the trapezoid body (MNTB) cells with the phospho-Ser503 Kv3.1 subunit antibody. The left panel shows control cells. The right panel shows cells that have been exposed to the protein kinase C activator PMA.](http://images.novusbio.com/fullsize/Kv3.1-[p-Ser503]-Antibody-Immunohistochemistry-NB300-279-img0001.jpg "Immunohistochemistry: Kv3.1 [p Ser503] Antibody [NB300-279] - IHC staining of medial nucleus of the trapezoid body (MNTB) cells with the phospho-Ser503 Kv3.1 subunit antibody. The left panel shows control cells. The right panel shows cells that have been exposed to the protein kinase C activator PMA.")
![Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p53-Antibody-Pab-DO-1-Western-Blot-NBP2-50538-img0001.jpg "Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.")
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ reduces HIF-1 alpha through different mechanisms in MCF-7 & MDA-MB-231. (A) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. After 24 hours, cells were treated with DCQ (5 μM) for 6 hours under hypoxia. Whole cell lysates were prepared, & blots were probed against indicated antibodies. (B) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. Transfected cells were treated with DCQ (5 μM) for 6 hours under hypoxia. The extent of DNA fragmentation was determined by TUNEL assay 24 hours later using flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (C) Whole cell lysates were prepared after pretreating MCF-7 & MDA-MB-231 with the proteasome inhibitor MG132 (3 μM) then treated with DCQ, & blots were probed for HIF-1 alpha . Results are from three independent experiments. (D) Whole cell lysates of MCF-7 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-AKT, mTOR, p-mTOR & GAPDH. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-310202416114753.jpg)
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ induces DNA damage & apoptosis via reactive oxygen species. (A) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-H2AX, p53, p-p53, p21, HIF-1 alpha & GAPDH. Results are representative of three independent experiments. (B) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 25 min incubation with 10 μM CM-H2DCFDA dye. Cells were washed with PBS & treated with DCQ for 1 hour under normoxia or hypoxia, after which cells were harvested & the amount of DCF fluorescence was analyzed by flow cytometry. Each percentage is the average ± SE of three independent experiments. (C) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 6 hours treatment with DCQ under normoxia or hypoxia. After 24 hours, the extent of DNA fragmentation was determined by TUNEL assay & measured by flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (D) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for HIF-1 alpha & GAPDH. Results are representative of three independent experiments. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-31020241612246.jpg)
![Flow Cytometry: Mouse Monoclonal p53 Antibody (Pab DO-1) [NBP2-50538] - P53 expression in monocyte subset of Human PBMCs post IFN gamma treatment. Image from a verified customer review. The customer used the Alexa Fluor® 647-conjugated version of NBP2-50538, which is listed under NBP2-50538AF647.](http://images.novusbio.com/fullsize/antibody/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-flow-cytometry-152025141858..png)
![Western Blot: APP Antibody (OTI4C9) - Azide and BSA Free [NBP2-70197] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APP.](http://images.novusbio.com/fullsize/APP-Antibody-OTI4C9-Azide-and-BSA-Free-Western-Blot-NBP2-70197-img0001.jpg "Western Blot: APP Antibody (OTI4C9) - Azide and BSA Free [NBP2-70197] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APP.")
![Immunohistochemistry: APP Antibody (OTI4C9) - Azide and BSA Free [NBP2-70197] - Analysis of Human lymphoma tissue.](http://images.novusbio.com/fullsize/APP-Antibody-OTI4C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70197-img0011.jpg)
![Immunohistochemistry: APP Antibody (OTI4C9) - Azide and BSA Free [NBP2-70197] - Analysis of Adenocarcinoma of Human breast tissue.](http://images.novusbio.com/fullsize/APP-Antibody-OTI4C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70197-img0002.jpg)
![Immunohistochemistry: APP Antibody (OTI4C9) - Azide and BSA Free [NBP2-70197] - Analysis of Adenocarcinoma of Human colon tissue.](http://images.novusbio.com/fullsize/APP-Antibody-OTI4C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70197-img0003.jpg)
![Immunohistochemistry: APP Antibody (OTI4C9) - Azide and BSA Free [NBP2-70197] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/APP-Antibody-OTI4C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70197-img0004.jpg)
![Immunohistochemistry: APP Antibody (OTI4C9) - Azide and BSA Free [NBP2-70197] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/APP-Antibody-OTI4C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70197-img0005.jpg)
![Immunohistochemistry: APP Antibody (OTI4C9) - Azide and BSA Free [NBP2-70197] - Analysis of Carcinoma of Human thyroid tissue.](http://images.novusbio.com/fullsize/APP-Antibody-OTI4C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70197-img0006.jpg)
![Immunohistochemistry: APP Antibody (OTI4C9) - Azide and BSA Free [NBP2-70197] - Analysis of Human breast tissue.](http://images.novusbio.com/fullsize/APP-Antibody-OTI4C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70197-img0007.jpg)
![Immunohistochemistry: APP Antibody (OTI4C9) - Azide and BSA Free [NBP2-70197] - Analysis of Human Kidney tissue.](http://images.novusbio.com/fullsize/APP-Antibody-OTI4C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70197-img0008.jpg)
![Immunohistochemistry: APP Antibody (OTI4C9) - Azide and BSA Free [NBP2-70197] - Analysis of Human liver tissue.](http://images.novusbio.com/fullsize/APP-Antibody-OTI4C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70197-img0009.jpg)
![Immunohistochemistry: APP Antibody (OTI4C9) - Azide and BSA Free [NBP2-70197] - Analysis of Human lung tissue.](http://images.novusbio.com/fullsize/APP-Antibody-OTI4C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70197-img0010.jpg)
![Immunohistochemistry: APP Antibody (OTI4C9) - Azide and BSA Free [NBP2-70197] - Analysis of Human pancreas tissue.](http://images.novusbio.com/fullsize/APP-Antibody-OTI4C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70197-img0012.jpg)
![Western Blot: beta-Catenin Antibody (OTI2F10) - Azide and BSA Free [NBP2-70511] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta-Catenin.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI2F10-Azide-and-BSA-Free-Western-Blot-NBP2-70511-img0001.jpg "Western Blot: beta-Catenin Antibody (OTI2F10) - Azide and BSA Free [NBP2-70511] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta-Catenin.")
![Immunohistochemistry: beta-Catenin Antibody (OTI2F10) - Azide and BSA Free [NBP2-70511] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI2F10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70511-img0007.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI2F10) - Azide and BSA Free [NBP2-70511] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI2F10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70511-img0002.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI2F10) - Azide and BSA Free [NBP2-70511] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI2F10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70511-img0003.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI2F10) - Azide and BSA Free [NBP2-70511] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI2F10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70511-img0004.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI2F10) - Azide and BSA Free [NBP2-70511] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI2F10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70511-img0005.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI2F10) - Azide and BSA Free [NBP2-70511] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI2F10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70511-img0006.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI2F10) - Azide and BSA Free [NBP2-70511] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI2F10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70511-img0008.jpg)
![Western Blot: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of DDIT3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Lane 1: LOVO cell lysate Lane 2: PC-12 cell lysate](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Western-Blot-NBP2-66856-img0007.jpg "Western Blot: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of DDIT3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Lane 1: LOVO cell lysate Lane 2: PC-12 cell lysate")
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded mouse pancreas tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0006.jpg)
![Flow Cytometry: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of Hela cells with DDIT3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Flow-Cytometry-NBP2-66856-img0001.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0002.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded human gastric carcinoma tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0003.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded human pancreas tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0004.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded mouse brain tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0005.jpg)
![Western Blot: CHMP2B Antibody [NBP2-15887] - Jurkat whole cell extracts and Jurkat exosome extract (15.4 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CHMP2B antibody diluted at 1:250. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CHMP2B-Antibody-Western-Blot-NBP2-15887-img0004.jpg "Western Blot: CHMP2B Antibody [NBP2-15887] - Jurkat whole cell extracts and Jurkat exosome extract (15.4 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CHMP2B antibody diluted at 1:250. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: CHMP2B Antibody [NBP2-15887] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/CHMP2B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15887-img0002.jpg)
![Immunohistochemistry-Paraffin: CHMP2B Antibody [NBP2-15887] - Paraffin-embedded human breast carcinoma. CHMP2B antibody diluted at 1:500.](http://images.novusbio.com/fullsize/CHMP2B-Antibody-Immunohistochemistry-Paraffin-NBP2-15887-img0003.jpg)
![Western Blot: CHMP2B Antibody [NBP2-15887] - Sample (30 ug of whole cell lysate) A: 293T B: A431 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/CHMP2B-Antibody-Western-Blot-NBP2-15887-img0001.jpg)
![Western Blot: CHMP2B Antibody [NBP2-15887] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CHMP2B antibody (NBP2-15887) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15887_rabbit-polyclonal-chmp2b-antibody-91020242011472.jpg)
![Western Blot: CHMP2B Antibody [NBP2-15887] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE NBP2-15887 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp2-15887_rabbit-polyclonal-chmp2b-antibody-910202420122019.jpg)