| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94448-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92783-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35721-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-42189-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12692-50ul | |||
![Western Blot: Somatostatin R5/SSTR5 Antibody (4O6N5) [NBP3-16682] - Analysis of extracts of various cell lines, using Somatostatin R5/SSTR5 Rabbit mAb (NBP3-16682) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/Somatostatin-R5-SSTR5-Antibody-4O6N5-Western-Blot-NBP3-16682-img0004.jpg "Western Blot: Somatostatin R5/SSTR5 Antibody (4O6N5) [NBP3-16682] - Analysis of extracts of various cell lines, using Somatostatin R5/SSTR5 Rabbit mAb (NBP3-16682) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunohistochemistry: Somatostatin R5/SSTR5 Antibody (4O6N5) [NBP3-16682] - Immunohistochemistry analysis of Somatostatin R5/SSTR5 in paraffin-embedded human liver cancer tissue using Somatostatin R5/SSTR5 Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16682_rabbit-somatostatin-r5-sstr5-mab-4o6n5-822025208477.jpg)
![Immunohistochemistry: Somatostatin R5/SSTR5 Antibody (4O6N5) [NBP3-16682] - Immunohistochemistry analysis of Somatostatin R5/SSTR5 in paraffin-embedded rat spleen tissue using Somatostatin R5/SSTR5 Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16682_rabbit-somatostatin-r5-sstr5-mab-4o6n5-822025208488.jpg)
![Immunohistochemistry: Somatostatin R5/SSTR5 Antibody (4O6N5) [NBP3-16682] - Immunohistochemistry analysis of Somatostatin R5/SSTR5 in paraffin-embedded mouse testis tissue using Somatostatin R5/SSTR5 Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16682_rabbit-somatostatin-r5-sstr5-mab-4o6n5-822025208476.jpg)
![Western Blot: MOK Antibody [NBP2-94448] - Analysis of extracts of mouse liver, using MOK at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/MOK-Antibody-Western-Blot-NBP2-94448-img0004.jpg "Western Blot: MOK Antibody [NBP2-94448] - Analysis of extracts of mouse liver, using MOK at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunohistochemistry-Paraffin: MOK Antibody [NBP2-94448] - Paraffin-embedded rat heart using MOK.](http://images.novusbio.com/fullsize/MOK-Antibody-Immunohistochemistry-Paraffin-NBP2-94448-img0003.jpg)
![Immunohistochemistry-Paraffin: MOK Antibody [NBP2-94448] - Paraffin-embedded human liver using MOK.](http://images.novusbio.com/fullsize/MOK-Antibody-Immunohistochemistry-Paraffin-NBP2-94448-img0001.jpg)
![Immunohistochemistry-Paraffin: MOK Antibody [NBP2-94448] - Paraffin-embedded mouse kidney using MOK.](http://images.novusbio.com/fullsize/MOK-Antibody-Immunohistochemistry-Paraffin-NBP2-94448-img0002.jpg)
![Western Blot: Kif4A Antibody (4A9P2) [NBP3-16748] - Western blot analysis of extracts of various cell lines, using Kif4A antibody (NBP3-16748) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Kif4A-Antibody-4A9P2-Western-Blot-NBP3-16748-img0004.jpg "Western Blot: Kif4A Antibody (4A9P2) [NBP3-16748] - Western blot analysis of extracts of various cell lines, using Kif4A antibody (NBP3-16748) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunohistochemistry-Paraffin: Kif4A Antibody (4A9P2) [NBP3-16748] - Immunohistochemistry of paraffin-embedded mouse testis using Kif4A Rabbit mAb (NBP3-16748) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Kif4A-Antibody-4A9P2-Immunohistochemistry-Paraffin-NBP3-16748-img0003.jpg)
![Immunohistochemistry-Paraffin: Kif4A Antibody (4A9P2) [NBP3-16748] - Immunohistochemistry of paraffin-embedded rat testis using Kif4A Rabbit mAb (NBP3-16748) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Kif4A-Antibody-4A9P2-Immunohistochemistry-Paraffin-NBP3-16748-img0001.jpg)
![Immunohistochemistry-Paraffin: Kif4A Antibody (4A9P2) [NBP3-16748] - Immunohistochemistry of paraffin-embedded human colon using Kif4A Rabbit mAb (NBP3-16748) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Kif4A-Antibody-4A9P2-Immunohistochemistry-Paraffin-NBP3-16748-img0002.jpg)
![Western Blot: CRCP Antibody [NBP2-92783] - Analysis of extracts of various cell lines, using CRCP .Exposure time: 90s.](http://images.novusbio.com/fullsize/CRCP-Antibody-Western-Blot-NBP2-92783-img0001.jpg "Western Blot: CRCP Antibody [NBP2-92783] - Analysis of extracts of various cell lines, using CRCP .Exposure time: 90s.")
![Immunohistochemistry-Paraffin: CRCP Antibody [NBP2-92783] - Immunohistochemistry of paraffin-embedded rat brain using CRCP Rabbit pAb (NBP2-92783) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CRCP-Antibody-Immunohistochemistry-Paraffin-NBP2-92783-img0004.jpg)
![Immunohistochemistry-Paraffin: CRCP Antibody [NBP2-92783] - Immunohistochemistry of paraffin-embedded human esophageal cancer using CRCP Rabbit pAb (NBP2-92783) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CRCP-Antibody-Immunohistochemistry-Paraffin-NBP2-92783-img0002.jpg)
![Immunohistochemistry-Paraffin: CRCP Antibody [NBP2-92783] - Immunohistochemistry of paraffin-embedded mouse lung using CRCP Rabbit pAb (NBP2-92783) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CRCP-Antibody-Immunohistochemistry-Paraffin-NBP2-92783-img0003.jpg)
![Immunohistochemistry: KIBRA Antibody [NBP3-35721] - Immunohistochemistry analysis of paraffin-embedded Rat ovary using KIBRA Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35721_rabbit-kibra-pab-912202414213750.jpg "Immunohistochemistry: KIBRA Antibody [NBP3-35721] - Immunohistochemistry analysis of paraffin-embedded Rat ovary using KIBRA Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: KIBRA Antibody [NBP3-35721] - Immunohistochemistry analysis of paraffin-embedded Human esophageal using KIBRA Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35721_rabbit-kibra-pab-91220241581236.jpg)
![Immunohistochemistry: KIBRA Antibody [NBP3-35721] - Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using KIBRA Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35721_rabbit-kibra-pab-912202414163044.jpg)
![Western Blot: KIBRA Antibody [NBP3-35721] - Western blot analysis of various lysates using KIBRA Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35721_rabbit-kibra-pab-912202415111869.jpg)
![Western Blot: SHANK3 Antibody (S367-51) [NBP2-42189] - Western Blot analysis of Monkey COS cells transfected with HA-tagged Shank1 showing detection of ~190 kDa SHANK1/SHANK3 protein using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone S367-51 (NBP2-42189). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with HA-tagged Shank1. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody (NBP2-42189) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~190 kDa.](http://images.novusbio.com/fullsize/SHANK3-Antibody-S367-51-Western-Blot-NBP2-42189-img0003.jpg "Western Blot: SHANK3 Antibody (S367-51) [NBP2-42189] - Western Blot analysis of Monkey COS cells transfected with HA-tagged Shank1 showing detection of ~190 kDa SHANK1/SHANK3 protein using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone S367-51 (NBP2-42189). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with HA-tagged Shank1. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody (NBP2-42189) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~190 kDa.")
![Immunocytochemistry/Immunofluorescence: SHANK3 Antibody (S367-51) [NBP2-42189] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone S367-51 (NBP2-42189). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody (NBP2-42189) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK1/SHANK3 Antibody (D) Composite.](http://images.novusbio.com/fullsize/SHANK3-Antibody-S367-51-Immunocytochemistry-Immunofluorescence-NBP2-42189-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SHANK3 Antibody (S367-51) [NBP2-42189] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/SHANK3-Antibody-S367-51-Immunocytochemistry-Immunofluorescence-NBP2-42189-img0002.jpg)
![Western Blot: Doublecortin Antibody (OTI4A3) - Azide and BSA Free [NBP2-70558] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Doublecortin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Doublecor](http://images.novusbio.com/fullsize/Doublecortin-Antibody-OTI4A3-Azide-and-BSA-Free-Western-Blot-NBP2-70558-img0001.jpg "Western Blot: Doublecortin Antibody (OTI4A3) - Azide and BSA Free [NBP2-70558] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Doublecortin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Doublecor")
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (OTI4A3) - Azide and BSA Free [NBP2-70558] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Doublecortin.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-OTI4A3-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70558-img0003.jpg)
![Flow Cytometry: Doublecortin Antibody (OTI4A3) - Azide and BSA Free [NBP2-70558] - Analysis of Jurkat cells, using anti-Doublecortin antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Doublecortin-Antibody-OTI4A3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70558-img0002.jpg)
![Western Blot: Dynamin Antibody (D5) [NB100-1993] - Analysis of chicken SL29 cell Lysate](http://images.novusbio.com/fullsize/Dynamin-Antibody-D5-Western-Blot-NB100-1993-img0003.jpg "Western Blot: Dynamin Antibody (D5) [NB100-1993] - Analysis of chicken SL29 cell Lysate")
![Western Blot: Dynamin Antibody (D5) [NB100-1993] - Western blot analysis of Dynamin, mAb (D5): Lane 1: MW marker, Lane 2: Mouse Brain, Lane 3: Rat Brain](http://images.novusbio.com/fullsize/Dynamin-Antibody-D5-Western-Blot-NB100-1993-img0001.jpg)
![Western Blot: AP2A2 Antibody [NBP3-12692] - Lane A: RT-4 Whole Cell LysateLane B: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:104 kDaObserved band size:104 kDa](http://images.novusbio.com/fullsize/AP2A2-Antibody-Western-Blot-NBP3-12692-img0001.jpg "Western Blot: AP2A2 Antibody [NBP3-12692] - Lane A: RT-4 Whole Cell LysateLane B: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:104 kDaObserved band size:104 kDa")
![Immunohistochemistry-Paraffin: AP2A2 Antibody [NBP3-12692] - Staining of human AP2A2 in human rectum with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/AP2A2-Antibody-Immunohistochemistry-Paraffin-NBP3-12692-img0003.jpg)
![Immunohistochemistry-Paraffin: AP2A2 Antibody [NBP3-12692] - Staining of human AP2A2 in human testis with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/AP2A2-Antibody-Immunohistochemistry-Paraffin-NBP3-12692-img0002.jpg)
![Immunoprecipitation: AP2A2 Antibody [NBP3-12692] - Lane A:0.5 mg RT-4 Whole Cell Lysate4 uL anti-AP2A2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-AP2A2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 104 kDaObserved band size :104 kDa](http://images.novusbio.com/fullsize/AP2A2-Antibody-Immunoprecipitation-NBP3-12692-img0004.jpg)
![Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunofluorescence analysis of paraffin-embedded human lymph nodes tissue labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32498, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-298202413585592.jpg "Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunofluorescence analysis of paraffin-embedded human lymph nodes tissue labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32498, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32498) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-29820241435258.jpg)
![Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunocytochemistry analysis of HeLa cells labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67/MKI67 antibody (NBP3-32498) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-2920241192339.jpg)
![Western Blot: DLG1 Antibody (JE39-74) [NBP3-32929] - Western blot analysis of DLG1 on different lysates with Rabbit anti-DLG1 antibody (NBP3-32929) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HepG2 cell lysate Lane 3: U-87 MG cell lysate Lane 4: SiHa cell lysate Lane 5: NCI-H226 cell lysate Lane 6: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 100 kDa Observed band size: 120 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32929) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32929_rabbit-dlg1-mab-je39-74-288202412442920.jpg "Western Blot: DLG1 Antibody (JE39-74) [NBP3-32929] - Western blot analysis of DLG1 on different lysates with Rabbit anti-DLG1 antibody (NBP3-32929) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HepG2 cell lysate Lane 3: U-87 MG cell lysate Lane 4: SiHa cell lysate Lane 5: NCI-H226 cell lysate Lane 6: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 100 kDa Observed band size: 120 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32929) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: DLG1 Antibody (JE39-74) [NBP3-32929] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-DLG1 antibody (NBP3-32929) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32929) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32929_rabbit-dlg1-mab-je39-74-298202414161898.jpg)
![Flow Cytometry: DLG1 Antibody (JE39-74) [NBP3-32929] - Flow cytometric analysis of SiHa cells labeling DLG1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32929, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32929_rabbit-dlg1-mab-je39-74-392024884663.jpg)
![Western Blot: Rac1,2,3 Antibody [NBP3-32887] - Western blot analysis of Rac1,2,3 on different lysates with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/500 dilution. Lane 1: Rat brain tissue lysate Lane 2: Human brain tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 5 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32887) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32887_rabbit-rac1-2-3-pab-288202412442915.jpg "Western Blot: Rac1,2,3 Antibody [NBP3-32887] - Western blot analysis of Rac1,2,3 on different lysates with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/500 dilution. Lane 1: Rat brain tissue lysate Lane 2: Human brain tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 5 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32887) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Rac1,2,3 Antibody [NBP3-32887] - Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32887) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32887_rabbit-rac1-2-3-pab-29820241420957.jpg)
![Immunocytochemistry/ Immunofluorescence: Rac1,2,3 Antibody [NBP3-32887] - Immunocytochemistry analysis of SH-SY5Y cells labeling Rac1,2,3 with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32887_rabbit-rac1-2-3-pab-29202410234241.jpg)