| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-82074-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87915-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84498-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44492 | |||
| BSA Free | NBP2-59779-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44057 | |||
| BSA Free | NBP2-61629-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84430-25ul | |||
![Western Blot: NFkB p105/p50 Antibody (5D10D11) [NBP2-22178] - Analysis using NFkB p105/p50 mouse mAb against K562 (1), Jurkat (2), A431 (3), Hela (4), THP-1 (5) and MCF-7 (6) cell lysate.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-5D10D11-Western-Blot-NBP2-22178-img0005.jpg "Western Blot: NFkB p105/p50 Antibody (5D10D11) [NBP2-22178] - Analysis using NFkB p105/p50 mouse mAb against K562 (1), Jurkat (2), A431 (3), Hela (4), THP-1 (5) and MCF-7 (6) cell lysate.")
![Immunocytochemistry/Immunofluorescence: NFkB p105/p50 Antibody (5D10D11) [NBP2-22178] - Analysis of U251 cells using NFkB p105/p50 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-5D10D11-Immunocytochemistry-Immunofluorescence-NBP2-22178-img0002.jpg)
![Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (5D10D11) [NBP2-22178] - Analysis of paraffin-embedded human intima cancer tissues (left) and human bladder cancer tissues (right) using NFkB p105/p50 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-5D10D11-Immunohistochemistry-Paraffin-NBP2-22178-img0003.jpg)
![Flow Cytometry: NFkB p105/p50 Antibody (5D10D11) [NBP2-22178] - Flow cytometric analysis of MCF-7 cells using NFkB p105/p50 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-5D10D11-Flow-Cytometry-NBP2-22178-img0001.jpg)
![Western Blot: NFkB p105/p50 Antibody (5D10D11) [NBP2-22178] - Analysis of A431 (A) and HeLa (B) cell lysate using NFkB p105/p50 (NBP2-22178) antibody at 1:500.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-5D10D11-Western-Blot-NBP2-22178-img0008.jpg)
![ELISA: NFkB p105/p50 Antibody (5D10D11) [NBP2-22178] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-5D10D11-ELISA-NBP2-22178-img0004.jpg)
![Western Blot: NFkB p105/p50 [p Ser337] Antibody [NB100-82074] - Analysis of extracts from Hela cells, treated with N oc or calf intestinal phosphates (CIP), using NFkB p105/p50 Antibody.](http://images.novusbio.com/fullsize/NFkB-p105-p50-[p-Ser337]-Antibody-Western-Blot-NB100-82074-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: NFkB p105/p50 [p Ser337] Antibody [NB100-82074] - Analysis of extracts from Hela cells, treated with N oc or calf intestinal phosphates (CIP), using NFkB p105/p50 Antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NFkB p105/p50 [p Ser337] Antibody [NB100-82074] - Staining of methanol-fixed HeLa cells.](http://images.novusbio.com/fullsize/NFkB-p105-p50-[p-Ser337]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-82074-img0005.jpg)
![Immunohistochemistry: NFkB p105/p50 [p Ser337] Antibody [NB100-82074] - Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using NB100-82074.](http://images.novusbio.com/fullsize/nb100-82074_rabbit-polyclonal-nfkb-p105-p50-p-ser337-antibody-10320252332101.jpg)
![Western Blot: NFkB p105/p50 [p Ser337] Antibody [NB100-82074] - Western blot analysis of extract from HeLa cells, using NB100-82074 (Lane 1 and 2).](http://images.novusbio.com/fullsize/nb100-82074_rabbit-polyclonal-nfkb-p105-p50-p-ser337-antibody-103202523472637.jpg)
![Western Blot: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of PEN2 on mouse spleen tissue lysate using anti-PEN2 antibody at 1/2,000 dilution.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Western-Blot-NBP2-75632-img0009.jpg "Western Blot: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of PEN2 on mouse spleen tissue lysate using anti-PEN2 antibody at 1/2,000 dilution.")
![Immunocytochemistry/Immunofluorescence: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Staining PEN2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunocytochemistry-Immunofluorescence-NBP2-75632-img0003.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded rat cerebellum tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0008.jpg)
![Flow Cytometry: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of A549 cells with PEN2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Flow-Cytometry-NBP2-75632-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Staining PEN2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunocytochemistry-Immunofluorescence-NBP2-75632-img0002.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded human lung cancer tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0004.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded human kidney tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0005.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded human tonsil tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0006.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded mouse testis tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0007.jpg)
![Western Blot: BICD1 Antibody [NBP1-78735] - Whole cell lysate from Jurkat (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and HeLa (H; 50 mcg) cells. Affinity purified rabbit anti-BICD1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. BICD1 was also immunoprecipitated by rabbit anti-BICD1 antibody NBP1-78734 which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/BICD1-Antibody-Western-Blot-NBP1-78735-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: BICD1 Antibody [NBP1-78735] - Whole cell lysate from Jurkat (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and HeLa (H; 50 mcg) cells. Affinity purified rabbit anti-BICD1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. BICD1 was also immunoprecipitated by rabbit anti-BICD1 antibody NBP1-78734 which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation. </b>Western Blot: BICD1 Antibody [NBP1-78735] - Effect of hypoxia on the interaction between HIF-1 alpha and BICD1. Cells were pretreated with MG132 (1 uM) for 30 min prior to hypoxia treatment for 24 h. Co-immunoprecipitation of HIF-1 alpha with IgG and BICD1 were shown in left panel. Total protein expressions in lysate were shown in right panel. n = 3. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41418-018-0241-1), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BICD1-Antibody-Western-Blot-NBP1-78735-img0004.jpg)
![Western Blot: BICD1 Antibody [NBP1-78735] - Role of BICD1 in UCB-MSCs survival under hypoxia. a, b The UCB-MSCs were transfected with BICD1 or NT siRNA for 24 h prior to hypoxia treatment. a Cells were exposed to various durations of hypoxia (0–72 h). Cell viability of UCB-MSCs was measured by WST-1 cell viability assay. n = 8. b Cells were incubated in normoxia or hypoxia conditions for 72 h. Representative images of experimental groups at 0, 24, 48, & 72 h of normoxia or hypoxia incubation are presented. Red-marked cells in representative images indicate PI-positive cells. n = 4. Scale bars are 50 μm (Magnification, × 200). cBICD1 or NT siRNA-transfected cells were incubated in hypoxia for 24 h. Cleaved caspase-9, cleaved caspase-3, & beta -Actin protein expressions were detected by western blot analysis. n = 4. d Cells were transfected with BICD1 or NT siRNA for 24 h prior to hypoxia treatment for 48 h. The percentages of apoptotic cells were analyzed by Annexin V/PI analysis, measured by flowcytometer. Annexin V-positive cells were considered as apoptotic cells. n = 4. Quantitative data are presented as a mean ± S.E.M. All blot images are representative. *p < 0.05 vs. normoxia control with NT siRNA transfection, #p < 0.05 vs. hypoxia with NT siRNA transfection. e The UCB-MSCs were transfected with pcDNA3.1/BICD1-cEGFP vector, pcDNA3.1/cEGFP vector, HIF1A siRNA or NT siRNA for 24 h prior to hypoxia treatment for 72 h. Cell viability data are presented as a mean ± S.E.M. n = 5. *p < 0.05 vs. normoxia control with pcDNA3.1/cEGFP vector & NT siRNA cotransfection, #p < 0.05 vs. hypoxia control with pcDNA3.1/cEGFP vector & NT siRNA cotransfection, @p < 0.05 vs. hypoxia control with pcDNA3.1/BICD1-cEGFP vector & NT siRNA cotransfection Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30464225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78735_rabbit-polyclonal-bicd1-antibody-310202416171413.jpg)
![Western Blot: BICD1 Antibody [NBP1-78735] - Effect of hypoxia on the interaction between HIF1 alpha & BICD1. a Co-immunoprecipitation of HIF1 alpha , Dynein IC, Importin alpha 3, RanBP2 w/ IgG & BICD1 antibodies shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. b Cells immunostained w/ HIF1 alpha & BICD1-specific antibodies. Scale bars are 8 μm (Magnification, × 1,000). White arrow heads indicate co-localization of HIF1 alpha w/ BICD1. c Interaction between HIF1 alpha & BICD1 (HIF1 alpha /BICD1, red) analyzed by PLA. Scale bars are 8 μm (Magnification, × 1,000). n = 6. *p < 0.05 vs. normoxia control. d–f Cells pretreated w/ MG132 (1 μM) for 30 min prior to hypoxia treatment for 24 h. d Co-immunoprecipitation of HIF1 alpha w/ IgG & BICD1 shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. e Cells immunostained w/ HIF1 alpha & BICD1-specific antibodies. White arrow heads indicate co-localization of HIF1 alpha w/ BICD1 in MG132-pretreated UCB-MSCs. Scale bars are 8 μm (Magnification, × 1,000). n = 5. f HIF1 activity measured by dual luciferase reporter assay. n = 6. *p < 0.05 vs. normoxia control w/ MG132 pretreatment. g, h Cells transfected w/ BICD1 or NT siRNA for 24 h prior to hypoxia treatment for 24 h. g Co-immunoprecipitation of Dynein IC w/ IgG & HIF1 alpha antibodies shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. h Interaction between HIF1 alpha & Dynein IC (HIF1 alpha /Dynein IC, red) analyzed by PLA. Scale bars are 8 μm (Magnification, × 1,000). Quantitative data are presented as a mean ± S.E.M. All blots & immunofluorescence images are representative. *p < 0.05 vs. normoxia control w/ NT siRNA transfection, #p < 0.05 vs. hypoxia w/ NT siRNA transfection Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30464225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78735_rabbit-polyclonal-bicd1-antibody-310202416171451.jpg)
![Western Blot: BICD1 Antibody [NBP1-78735] - Effect of hypoxia on the interaction between HIF1 alpha & BICD1. a Co-immunoprecipitation of HIF1 alpha , Dynein IC, Importin alpha 3, RanBP2 w/ IgG & BICD1 antibodies shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. b Cells immunostained w/ HIF1 alpha & BICD1-specific antibodies. Scale bars are 8 μm (Magnification, × 1,000). White arrow heads indicate co-localization of HIF1 alpha w/ BICD1. c Interaction between HIF1 alpha & BICD1 (HIF1 alpha /BICD1, red) analyzed by PLA. Scale bars are 8 μm (Magnification, × 1,000). n = 6. *p < 0.05 vs. normoxia control. d–f Cells pretreated w/ MG132 (1 μM) for 30 min prior to hypoxia treatment for 24 h. d Co-immunoprecipitation of HIF1 alpha w/ IgG & BICD1 shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. e Cells immunostained w/ HIF1 alpha & BICD1-specific antibodies. White arrow heads indicate co-localization of HIF1 alpha w/ BICD1 in MG132-pretreated UCB-MSCs. Scale bars are 8 μm (Magnification, × 1,000). n = 5. f HIF1 activity measured by dual luciferase reporter assay. n = 6. *p < 0.05 vs. normoxia control w/ MG132 pretreatment. g, h Cells transfected w/ BICD1 or NT siRNA for 24 h prior to hypoxia treatment for 24 h. g Co-immunoprecipitation of Dynein IC w/ IgG & HIF1 alpha antibodies shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. h Interaction between HIF1 alpha & Dynein IC (HIF1 alpha /Dynein IC, red) analyzed by PLA. Scale bars are 8 μm (Magnification, × 1,000). Quantitative data are presented as a mean ± S.E.M. All blots & immunofluorescence images are representative. *p < 0.05 vs. normoxia control w/ NT siRNA transfection, #p < 0.05 vs. hypoxia w/ NT siRNA transfection Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30464225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78735_rabbit-polyclonal-bicd1-antibody-31020241616184.jpg)
![Western Blot: BICD1 Antibody [NBP1-78735] - Involvement of Akt/GSK3 beta pathway in BICD1-mediated HIF1 alpha nuclear translocation. a–e The UCB-MSCs were transfected with GSK3 beta or NT siRNA for 24 h prior to hypoxia treatment for 24 h. a Co-immunoprecipitation of HIF1 alpha & Dynein IC with IgG & BICD1 antibodies were shown in left panel. Total protein expressions in lysate were shown in right panel. n = 3. b Interaction between HIF1 alpha & BICD1 (HIF1 alpha /BICD1, red) was analyzed by PLA assay. Scale bars are 8 μm (Magnification, × 1,000). n = 5. c HIF1 alpha , Lamin A/C, & alpha -Tubulin in cytosolic & nuclear fractionized samples were detected by western blot. n = 4. d Cells were immunostained with HIF1 alpha -specific antibody. Scale bars are 8 μm (Magnification, × 1,000). n = 4. e HIF1 activities in NT or GSK3 beta siRNA-transfected cells were analyzed by dual luciferase reporter assay. n = 6. Quantitative data are presented as a mean ± S.E.M. All blots & immunofluorescence images are representative. *p < 0.05 vs. normoxia control with NT siRNA transfection, #p < 0.05 vs. hypoxia with NT siRNA transfection Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30464225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78735_rabbit-polyclonal-bicd1-antibody-31020241616183.jpg)
![Western Blot: Abeta 40 Antibody [NBP1-44047] - 1-40(lane 1) and 1-42 (lane 2) ABeta peptides were run on a 16.5% TRIS/tricine gel and transferred to PVDF membrane. The peptide concentration was approximately 0.5ng/lane. The blot was incubated with affinity purified AB40 antibody at 1ug/ml, followed by an oat and rabbit -HRP antibody at 1:40,000 and developed using a standard chemiluminescence method. A single band is present only in lane 1 which contains the ABeta 1-40 peptide and no signal is found in lane 2 which contains the ABeta 1-42 peptide.](http://images.novusbio.com/fullsize/Abeta-40-Antibody-Western-Blot-NBP1-44047-img0002.jpg "Western Blot: Abeta 40 Antibody [NBP1-44047] - 1-40(lane 1) and 1-42 (lane 2) ABeta peptides were run on a 16.5% TRIS/tricine gel and transferred to PVDF membrane. The peptide concentration was approximately 0.5ng/lane. The blot was incubated with affinity purified AB40 antibody at 1ug/ml, followed by an oat and rabbit -HRP antibody at 1:40,000 and developed using a standard chemiluminescence method. A single band is present only in lane 1 which contains the ABeta 1-40 peptide and no signal is found in lane 2 which contains the ABeta 1-42 peptide.")
![Immunocytochemistry/Immunofluorescence: Mena Antibody [NBP1-87915] - Staining of human cell line U-2 OS shows localization to plasma membrane, cytosol & focal adhesion sites. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Mena-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87915-img0006.jpg "Immunocytochemistry/Immunofluorescence: Mena Antibody [NBP1-87915] - Staining of human cell line U-2 OS shows localization to plasma membrane, cytosol & focal adhesion sites. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915] - Analysis in human testis and liver tissues. Corresponding ENAH RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Mena-Antibody-Immunohistochemistry-Paraffin-NBP1-87915-img0017.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915] - Staining of human endometrium, liver, pancreas and tonsil using Anti-AGXT antibody (A) NBP1-87915 shows similar protein distribution across tissues to independent antibody NBP1-87914 (B).](http://images.novusbio.com/fullsize/Mena-Antibody-Immunohistochemistry-Paraffin-NBP1-87915-img0021.jpg)
![Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915] - Staining of human prostate shows moderate to strong cytoplasmic and membranous positivity in glandular cells and smooth muscle cells.](http://images.novusbio.com/fullsize/Mena-Antibody-Immunohistochemistry-Paraffin-NBP1-87915-img0023.jpg)
![Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915] - Staining of human Fallopian tube shows strong cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Mena-Antibody-Immunohistochemistry-Paraffin-NBP1-87915-img0018.jpg)
![Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915] - Staining of human liver shows low positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Mena-Antibody-Immunohistochemistry-Paraffin-NBP1-87915-img0019.jpg)
![Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Mena-Antibody-Immunohistochemistry-Paraffin-NBP1-87915-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: CLE7 homolog Antibody [NBP1-84498] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/CLE7-homolog-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84498-img0008.jpg "Immunocytochemistry/Immunofluorescence: CLE7 homolog Antibody [NBP1-84498] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.")
![Western Blot: GAPDH Antibody (CL3266) [NBP2-59779] - GAPDH Antibody (2597) [NBP2-59779] - Theoretical molecular weight: 36 kDa. Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-GAPDH monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3266-Western-Blot-NBP2-59779-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GAPDH Antibody (CL3266) [NBP2-59779] - GAPDH Antibody (2597) [NBP2-59779] - Theoretical molecular weight: 36 kDa. Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-GAPDH monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: GAPDH Antibody (CL3266) [NBP2-59779] - Staining of A549 cells using the Anti-GAPDH monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3266-Immunocytochemistry-Immunofluorescence-NBP2-59779-img0013.jpg)
![Western Blot: GAPDH Antibody (CL3266) [NBP2-59779] - GAPDH Antibody (2597) [NBP2-59779] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431 and human cell line HepG2. Theoretical molecular weight: 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3266-Western-Blot-NBP2-59779-img0008.jpg)
![Immunohistochemistry: GAPDH Antibody (CL3266) [NBP2-59779] - Human kidney shows cytoplasmic and nuclear immunoreactivity in renal tubules and glomerular cells.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3266-Immunohistochemistry-NBP2-59779-img0002.jpg)
![Immunohistochemistry: GAPDH Antibody (CL3266) [NBP2-59779] - Human fallopian tube shows cytoplasmic and nuclear positivity in the epithelial and connective tissue cells.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3266-Immunohistochemistry-NBP2-59779-img0003.jpg)
![Immunohistochemistry: GAPDH Antibody (CL3266) [NBP2-59779] - Human liver shows cytoplasmic and nuclear immunoreactivity in hepatocytes.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3266-Immunohistochemistry-NBP2-59779-img0004.jpg)
![Immunohistochemistry: GAPDH Antibody (CL3266) [NBP2-59779] - Human pancreas shows nuclear and cytoplasmic immunoreactivity in both endocrine and exocrine cells.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3266-Immunohistochemistry-NBP2-59779-img0005.jpg)
![Western Blot: beta-Catenin Antibody (GT2169) [NBP3-13530] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Catenin antibody [GT2169] (NBP3-13530) diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-GT2169-Western-Blot-NBP3-13530-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: beta-Catenin Antibody (GT2169) [NBP3-13530] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Catenin antibody [GT2169] (NBP3-13530) diluted at 1:500.")
![<b>Genetic Strategies Validation. </b>Western Blot: beta-Catenin Antibody (GT2169) [NBP3-13530] - Wild-type (WT) and beta-Catenin knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Catenin antibody [GT2169] (NBP3-13530) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-GT2169-Western-Blot-NBP3-13530-img0004.jpg)
![Western Blot: beta-Catenin Antibody (GT2169) [NBP3-13530] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Catenin antibody (NBP3-13530) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-13530_mouse-beta-catenin-mab-gt2169-91020242091497.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (GT2169) [NBP3-13530] - beta-Catenin antibody detects beta-Catenin protein at cell membrane by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: beta-Catenin stained by beta-Catenin antibody (NBP3-13530) diluted at 1:500.](http://images.novusbio.com/fullsize/nbp3-13530_mouse-beta-catenin-mab-gt2169-910202420131215.jpg)
![beta Catenin antibody [GT2169] detects beta Catenin protein at cell membrane by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: beta Catenin protein stained by beta Catenin antibody [GT2169] (NBP3-13530) diluted at 1:200.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp3-13530_mouse-beta-catenin-mab-gt2169-immunocytochemistry-immunofluorescence-2611202518375021.jpg)
![beta Catenin antibody [GT2169] detects beta Catenin protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.beta Catenin stained by beta Catenin antibody [GT2169] (NBP3-13530) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13530_mouse-beta-catenin-mab-gt2169-immunohistochemistry-paraffin-261120251843450.jpg)
![beta Catenin antibody [GT2169] detects beta Catenin protein by immunohistochemical analysis.Sample: Paraffin-embedded human pancreas.beta Catenin stained by beta Catenin antibody [GT2169] (NBP3-13530) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13530_mouse-beta-catenin-mab-gt2169-immunohistochemistry-paraffin-26112025196218.jpg)
![beta Catenin antibody [GT2169] detects beta Catenin protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.beta Catenin stained by beta Catenin antibody [GT2169] (NBP3-13530) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13530_mouse-beta-catenin-mab-gt2169-immunohistochemistry-paraffin-26112025184740.jpg)
![beta Catenin antibody [GT2169] detects beta Catenin protein by immunohistochemical analysis.Sample: Paraffin-embedded human appendix.beta Catenin stained by beta Catenin antibody [GT2169] (NBP3-13530) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13530_mouse-beta-catenin-mab-gt2169-immunohistochemistry-paraffin-261120251829266.jpg)
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [GT2169] (NBP3-13530) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-13530_mouse-beta-catenin-mab-gt2169-western-blot-2611202518372424.jpg)
![Lane 1: Marker [kDa]Lane 2: Negative control (vector only transfected HEK293T lysate)�Lane 3: MEF2C Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419349)](http://images.novusbio.com/fullsize/nbp3-52062_mouse-mef2c-mab-cl0368-western-blot-27320261528200.jpg "Lane 1: Marker [kDa]Lane 2: Negative control (vector only transfected HEK293T lysate)�Lane 3: MEF2C Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419349)")
![Western Blot: beta-Catenin Antibody (CL3691) [NBP2-61629] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line A-549](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3691-Western-Blot-NBP2-61629-img0011.jpg "Western Blot: beta-Catenin Antibody (CL3691) [NBP2-61629] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line A-549")
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (CL3691) [NBP2-61629] - Staining of A-431 cells using the Anti-CTNNB1 monoclonal antibody, showing specific staining in the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3691-Immunocytochemistry-Immunofluorescence-NBP2-61629-img0012.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CL3691) [NBP2-61629] - Staining of human skeletal muscle shows absence of immunoreactivity in muscle fibers (negative control).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3691-Immunohistochemistry-Paraffin-NBP2-61629-img0009.jpg)
![Western Blot: beta-Catenin Antibody (CL3691) [NBP2-61629] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line SK-MEL-30](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3691-Western-Blot-NBP2-61629-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (CL3691) [NBP2-61629] - Staining of U-251 cells using the Anti-CTNNB1 monoclonal antibody, showing specific staining in the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3691-Immunocytochemistry-Immunofluorescence-NBP2-61629-img0013.jpg)
![Immunohistochemistry: beta-Catenin Antibody (CL3691) [NBP2-61629] - Staining of human small intestine shows strong membranous immunoreactivity in the epithelial cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3691-Immunohistochemistry-NBP2-61629-img0001.jpg)
![Immunohistochemistry: beta-Catenin Antibody (CL3691) [NBP2-61629] - Staining of human cervix shows strong membranous positivity in epithelial cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3691-Immunohistochemistry-NBP2-61629-img0002.jpg)
![Immunohistochemistry: beta-Catenin Antibody (CL3691) [NBP2-61629] - Staining of human prostate shows membranous immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3691-Immunohistochemistry-NBP2-61629-img0003.jpg)
![Immunohistochemistry: beta-Catenin Antibody (CL3691) [NBP2-61629] - Staining of human fallopian tube shows strong membranous immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3691-Immunohistochemistry-NBP2-61629-img0004.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CL3691) [NBP2-61629] - Staining of human placenta shows strong membranous positivity in trophoblast.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3691-Immunohistochemistry-Paraffin-NBP2-61629-img0005.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CL3691) [NBP2-61629] - Staining of human prostate cancer shows membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3691-Immunohistochemistry-Paraffin-NBP2-61629-img0006.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CL3691) [NBP2-61629] - Staining of human breast cancer shows membranous immunoreactivity in tumor cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3691-Immunohistochemistry-Paraffin-NBP2-61629-img0007.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CL3691) [NBP2-61629] - Staining of human renal cancer shows membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3691-Immunohistochemistry-Paraffin-NBP2-61629-img0008.jpg)
![Western Blot: GDAP1 Antibody [NBP1-84430] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissue](http://images.novusbio.com/fullsize/GDAP1-Antibody-Western-Blot-NBP1-84430-img0004.jpg "Western Blot: GDAP1 Antibody [NBP1-84430] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissue")
![Immunocytochemistry/Immunofluorescence: GDAP1 Antibody [NBP1-84430] - Immunofluorescent staining of human cell line A549 shows localization to cytosol & mitochondria. Antibody staining shown in green.](http://images.novusbio.com/fullsize/GDAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84430-img0006.jpg)
![Immunohistochemistry-Paraffin: GDAP1 Antibody [NBP1-84430] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/GDAP1-Antibody-Immunohistochemistry-Paraffin-NBP1-84430-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GDAP1 Antibody [NBP1-84430] - Staining in human cerebral cortex and pancreas tissues using anti-GDAP1 antibody. Corresponding GDAP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GDAP1-Antibody-Immunohistochemistry-Paraffin-NBP1-84430-img0009.jpg)
