| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50050SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99063-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87709-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-45090 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22328-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-16996-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80778-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08322 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03675-20ul | |||
![Western Blot: Calbindin D-28K Antibody (5A9) [NBP2-50050] - Analysis of different neuronal tissue lysates using mouse mAb to Calbindin D-28K, dilution 1:5,000: [1] protein standard, [2] rat cerebellum, [3] pig hippocampus, and [4] cow cerebellum. Bands at about 26kDa corresponds to the calbindin protein, heavily expressed in the cerebellum but a very minor component of hippocampus.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-5A9-Western-Blot-NBP2-50050-img0004.jpg "Western Blot: Calbindin D-28K Antibody (5A9) [NBP2-50050] - Analysis of different neuronal tissue lysates using mouse mAb to Calbindin D-28K, dilution 1:5,000: [1] protein standard, [2] rat cerebellum, [3] pig hippocampus, and [4] cow cerebellum. Bands at about 26kDa corresponds to the calbindin protein, heavily expressed in the cerebellum but a very minor component of hippocampus.")
![Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody (5A9) [NBP2-50050] - Adult mouse cortex (Left) and cerebellum (Right) sections were stained with NBP2-50050 in red, and co-stained with rabbit polyclonal anti-RBFOX3/NeuN antibody (NBP1-92716, green). Calbindin is expressed in a subset of interneurons in the cortex (Left) and prominently expressed in the dendrites of Purkinje cells in the cerebellum molecular layer (Right). RBFOX3/NeuN expresses in most neurons; as a result, cells positive for Calbindin appear to be yellow. Insets are high magnification images of the boxed area. Blue is Dapi nucleus staining that labels DNA.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-5A9-Immunocytochemistry-Immunofluorescence-NBP2-50050-img0003.jpg)
![Immunohistochemistry Free-Floating: Calbindin D-28K Antibody (5A9) [NBP2-50050] - Analysis of rat brain cerebellum section stained with mouse mAb to Calbindin D-28K, dilution 1:2,000 (Red), and costained with rabbit GFAP pAb, dilution 1:5,000 (Green). DAPI staining of nuclear DNA (Blue). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with the above antibodies. The Calbindin D-28K antibody prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum. The GFAP antibody stains the processes of Bergmann glia in the molecular layer and astroglia in the granular and white matter layers of cerebellum.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-5A9-Immunohistochemistry-Free-Floating-NBP2-50050-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: Calbindin D-28K Antibody (1K2K2) [NBP3-16209] - Confocal imaging of paraffin-embedded Mouse brain tissue using Calbindin D-28K Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16209_rabbit-calbindin-d-28k-mab-1k2k2-72202512435372.jpg)
![Western Blot: Calbindin D-28K Antibody [NBP2-99063] - Anti-Calbindin D-28K rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse kidney tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 27 kDa](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Western-Blot-NBP2-99063-img0003.jpg "Western Blot: Calbindin D-28K Antibody [NBP2-99063] - Anti-Calbindin D-28K rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse kidney tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 27 kDa")
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP2-99063] - Immunochemical staining of human Calbindin D-28K in human brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-Paraffin-NBP2-99063-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody [NBP2-99063] - Immunofluorescence staining of Calbindin D-28K in 293 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-99063 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-99063_rabbit-polyclonal-calbindin-d-28k-antibody-235202514331592.png)
![Immunoprecipitation: Calbindin D-28K Antibody [NBP2-99063] - Calbindin D-28K was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate. 4 ul NBP2-99063 and 60 ug of Immunomagnetic beads. Protein G. Primary antibody: NBP2-99063, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa](http://images.novusbio.com/fullsize/nbp2-99063_rabbit-polyclonal-calbindin-d-28k-antibody-235202516335024.png)
![Western Blot: ADAMTS4 Antibody [NBP1-54369] - Sample Tissue: Human Fetal Lung Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/ADAMTS4-Antibody-Western-Blot-NBP1-54369-img0005.jpg "Western Blot: ADAMTS4 Antibody [NBP1-54369] - Sample Tissue: Human Fetal Lung Antibody Dilution: 1.0 ug/ml")
![Immunohistochemistry-Paraffin: ADAMTS4 Antibody [NBP1-54369] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/ADAMTS4-Antibody-Immunohistochemistry-Paraffin-NBP1-54369-img0004.jpg)
![Western Blot: ADAMTS4 Antibody [NBP1-54369] - HepG2 cell lysate, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/ADAMTS4-Antibody-Western-Blot-NBP1-54369-img0002.jpg)
![Western Blot: Kir2.1 Antibody (JE54-56) [NBP3-32505] - Western blot analysis of Kir2.1 on 293 cell lysates with Rabbit anti-Kir2.1 antibody (NBP3-32505) at 1/1,000 dilution. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 30s; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32505) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32505_rabbit-kir2-1-mab-je54-56-278202415295538.jpg "Western Blot: Kir2.1 Antibody (JE54-56) [NBP3-32505] - Western blot analysis of Kir2.1 on 293 cell lysates with Rabbit anti-Kir2.1 antibody (NBP3-32505) at 1/1,000 dilution. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 30s; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32505) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Kir2.1 Antibody (JE54-56) [NBP3-32505] - Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-Kir2.1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32505, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32505_rabbit-kir2-1-mab-je54-56-298202414333623.jpg)
![Immunocytochemistry/ Immunofluorescence: Kir2.1 Antibody (JE54-56) [NBP3-32505] - ICC staining of Kir2.1 in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP3-32505, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32505_rabbit-kir2-1-mab-je54-56-29202410484869.jpg)
![Immunohistochemistry-Paraffin: Kir2.1 Antibody [NBP1-87709] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/Kir2.1-Antibody-Immunohistochemistry-Paraffin-NBP1-87709-img0007.jpg "Immunohistochemistry-Paraffin: Kir2.1 Antibody [NBP1-87709] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.")
![Immunohistochemistry: Kir2.1 Antibody [NBP1-87709] - Staining of human colon shows strong cytoplasmic and membrane positivity in glandular cells.](http://images.novusbio.com/fullsize/Kir2.1-Antibody-Immunohistochemistry-NBP1-87709-img0004.jpg)
![Immunohistochemistry-Paraffin: Kir2.1 Antibody [NBP1-87709] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Kir2.1-Antibody-Immunohistochemistry-Paraffin-NBP1-87709-img0005.jpg)
![Immunohistochemistry-Paraffin: Kir2.1 Antibody [NBP1-87709] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Kir2.1-Antibody-Immunohistochemistry-Paraffin-NBP1-87709-img0006.jpg)
![Immunohistochemistry-Paraffin: Kir2.1 Antibody [NBP1-87709] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Kir2.1-Antibody-Immunohistochemistry-Paraffin-NBP1-87709-img0008.jpg)
![Western Blot: Kir2.1 Antibody (SR1939) [NBP3-22328] - Western blot analysis using NBP3-22328 in A549 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22328_rabbit-kir2-1-mab-sr1939-57202315545127.jpg "Western Blot: Kir2.1 Antibody (SR1939) [NBP3-22328] - Western blot analysis using NBP3-22328 in A549 cell lysate.")
![Immunohistochemistry-Paraffin: PTH1R/PTHR1 Antibody [NBP3-16996] - Staining of human kidney shows high expression.](http://images.novusbio.com/fullsize/PTH1R-PTHR1-Antibody-Immunohistochemistry-Paraffin-NBP3-16996-img0002.jpg "Immunohistochemistry-Paraffin: PTH1R/PTHR1 Antibody [NBP3-16996] - Staining of human kidney shows high expression.")
![Immunohistochemistry-Paraffin: PTH1R/PTHR1 Antibody [NBP3-16996] - Analysis in human kidney and tonsil tissues using Anti-PTH1R antibody. Corresponding PTH1R RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PTH1R-PTHR1-Antibody-Immunohistochemistry-Paraffin-NBP3-16996-img0001.jpg)
![Immunohistochemistry-Paraffin: PTH1R/PTHR1 Antibody [NBP3-16996] - Staining of human tonsil shows low expression as expected.](http://images.novusbio.com/fullsize/PTH1R-PTHR1-Antibody-Immunohistochemistry-Paraffin-NBP3-16996-img0003.jpg)
![Immunohistochemistry-Paraffin: SCRG1 Antibody [NBP1-80778] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/SCRG1-Antibody-Immunohistochemistry-Paraffin-NBP1-80778-img0002.jpg "Immunohistochemistry-Paraffin: SCRG1 Antibody [NBP1-80778] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.")
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (CALB1/3333) [NBP3-07364] - Formalin-fixed, paraffin-embedded human Kidney stained with Calbindin D-28K Mouse Monoclonal Antibody (CALB1/3333).](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-CALB1-3333-Immunohistochemistry-Paraffin-NBP3-07364-img0003.jpg "Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (CALB1/3333) [NBP3-07364] - Formalin-fixed, paraffin-embedded human Kidney stained with Calbindin D-28K Mouse Monoclonal Antibody (CALB1/3333).")
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (CALB1/3333) [NBP3-07364] - Formalin-fixed, paraffin-embedded human Kidney stained with Calbindin D-28K Mouse Monoclonal Antibody (CALB1/3333).](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-CALB1-3333-Immunohistochemistry-Paraffin-NBP3-07364-img0002.jpg)
![Protein Array: Calbindin D-28K Antibody (CALB1/3333) [NBP3-07364] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Calbindin D-28K Mouse Monoclonal Antibody (CALB1/3333).](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-CALB1-3333-Protein-Array-NBP3-07364-img0001.jpg)
![Western Blot: Kir2.1 Antibody [NBP3-03675] - Analysis of extracts of various cell lines, using Kir2.1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/Kir2.1-Antibody-Western-Blot-NBP3-03675-img0001.jpg "Western Blot: Kir2.1 Antibody [NBP3-03675] - Analysis of extracts of various cell lines, using Kir2.1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")