| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89893-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27833-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33274-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80903-25ul | |||
![Western Blot: PIEZO1 Antibody [NBP2-10504] - PC2, TRPV4, & PIEZO1 localize to MLO-Y4 primary cilia & plasma membrane. (A–C) Localization of PC2, TRPV4, & PIEZO1 on MLO-Y4 primary cilia & plasma membrane. Acetylated alpha-tubulin is in enriched primary cilia. Scale bars, 10 μm. (D) Chemiluminescence-detected Western blot of PC2, TRPV4, PIEZO1, & actin protein in MLO-Y4s treated with scrambled control siRNA & Pkd2, Trpv4, or Piezo1 siRNA. (E) Quantified knockdown of Pkd2 (n = 4), Trpv4 (n = 10), & Piezo1 (n = 4) mRNA expression in MLO-Y4 cells treated with siRNA relative to controls (n = 10). Error bars show ±SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26029358), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-10504_rabbit-polyclonal-piezo1-antibody-31020241537199.jpg "<b>Genetic Strategies Validation. </b>Western Blot: PIEZO1 Antibody [NBP2-10504] - PC2, TRPV4, & PIEZO1 localize to MLO-Y4 primary cilia & plasma membrane. (A–C) Localization of PC2, TRPV4, & PIEZO1 on MLO-Y4 primary cilia & plasma membrane. Acetylated alpha-tubulin is in enriched primary cilia. Scale bars, 10 μm. (D) Chemiluminescence-detected Western blot of PC2, TRPV4, PIEZO1, & actin protein in MLO-Y4s treated with scrambled control siRNA & Pkd2, Trpv4, or Piezo1 siRNA. (E) Quantified knockdown of Pkd2 (n = 4), Trpv4 (n = 10), & Piezo1 (n = 4) mRNA expression in MLO-Y4 cells treated with siRNA relative to controls (n = 10). Error bars show ±SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26029358), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: CRCP Antibody (JE65-42) [NBP3-32218] - Western blot analysis of CRCP on MCF-7 cell lysates with Rabbit anti-CRCP antibody (NBP3-32218) at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32218) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32218_rabbit-crcp-mab-je65-42-278202415295520.jpg "Western Blot: CRCP Antibody (JE65-42) [NBP3-32218] - Western blot analysis of CRCP on MCF-7 cell lysates with Rabbit anti-CRCP antibody (NBP3-32218) at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32218) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CRCP Antibody (JE65-42) [NBP3-32218] - Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-CRCP antibody (NBP3-32218) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32218) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32218_rabbit-crcp-mab-je65-42-298202414161851.jpg)
![Immunocytochemistry/ Immunofluorescence: CRCP Antibody (JE65-42) [NBP3-32218] - Immunocytochemistry analysis of SH-SY5Y cells labeling CRCP with Rabbit anti-CRCP antibody (NBP3-32218) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CRCP antibody (NBP3-32218) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32218_rabbit-crcp-mab-je65-42-2920241033408.jpg)
![Western Blot: CRCP Antibody (SR4283) [NBP3-27833] - Western blot analysis of CRCP expression in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-27833_rabbit-crcp-mab-sr4283-75202410133483.jpg "Western Blot: CRCP Antibody (SR4283) [NBP3-27833] - Western blot analysis of CRCP expression in HeLa cell lysate.")
![Immunocytochemistry/Immunofluorescence: DOK4 Antibody [NBP2-33274] - Immunofluorescent staining of human cell line CACO-2 shows localization to microtubules & cytokinetic bridge.](http://images.novusbio.com/fullsize/DOK4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33274-img0005.jpg "Immunocytochemistry/Immunofluorescence: DOK4 Antibody [NBP2-33274] - Immunofluorescent staining of human cell line CACO-2 shows localization to microtubules & cytokinetic bridge.")
![Immunohistochemistry-Paraffin: DOK4 Antibody [NBP2-33274] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/DOK4-Antibody-Immunohistochemistry-Paraffin-NBP2-33274-img0006.jpg)
![Immunohistochemistry-Paraffin: EGR3 Antibody [NBP1-80903] - Staining of human duodenum shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/EGR3-Antibody-Immunohistochemistry-Paraffin-NBP1-80903-img0004.jpg "Immunohistochemistry-Paraffin: EGR3 Antibody [NBP1-80903] - Staining of human duodenum shows moderate granular cytoplasmic positivity in glandular cells.")
![Western Blot: GluR5/GRIK1 Antibody [NB100-68165] - (1ug/ml) staining of Human Brain (Cerebellum) 35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/GluR5-GRIK1-Antibody-Western-Blot-NB100-68165-img0003.jpg "Western Blot: GluR5/GRIK1 Antibody [NB100-68165] - (1ug/ml) staining of Human Brain (Cerebellum) 35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: EGR3 Antibody [NBP2-82982] - WB Suggested Anti-EGR3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Spleen](http://images.novusbio.com/fullsize/EGR3-Antibody-Western-Blot-NBP2-82982-img0001.jpg "Western Blot: EGR3 Antibody [NBP2-82982] - WB Suggested Anti-EGR3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Spleen")
![Western Blot: EGR3 Antibody [NBP2-82982] - Host: Rabbit. Target: EGR3. Positive control (+): Mouse Brain (M-BR). Negative control (-): Mouse Small Intestine (M-IN). Antibody concentration: 2ug/ml](http://images.novusbio.com/fullsize/EGR3-Antibody-Western-Blot-NBP2-82982-img0002.jpg)