| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43805 | |||
| BSA Free | NBP2-46643-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92928-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14023 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26491-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14039 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-24286 | |||
![Western Blot: Glutamine Synthetase Antibody (2999) [NBP2-46643] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-2999-Western-Blot-NBP2-46643-img0006.jpg "Western Blot: Glutamine Synthetase Antibody (2999) [NBP2-46643] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue")
![Immunohistochemistry: Glutamine Synthetase Antibody (2999) [NBP2-46643] - Staining of human pancreas shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-2999-Immunohistochemistry-NBP2-46643-img0004.jpg)
![Western Blot: Glutamine Synthetase Antibody (2999) [NBP2-46643] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-2999-Western-Blot-NBP2-46643-img0005.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (2999) [NBP2-46643] - Staining of rat cerebral cortex shows immunoreactivity in a subset of astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-2999-Immunohistochemistry-NBP2-46643-img0001.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (2999) [NBP2-46643] - Staining of mouse hippocampal formation shows positivity in a subset of astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-2999-Immunohistochemistry-NBP2-46643-img0002.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (2999) [NBP2-46643] - Staining of human cerebral cortex shows strong immunoreactivity in astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-2999-Immunohistochemistry-NBP2-46643-img0003.jpg)
![Western Blot: ATP5A Antibody [NBP2-38525] - Analysis using Anti-ATP5A1 antibody NBP2-38525 (A) shows similar pattern to independent antibody NBP2-38470 (B).](http://images.novusbio.com/fullsize/ATP5A-Antibody-Western-Blot-NBP2-38525-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: ATP5A Antibody [NBP2-38525] - Analysis using Anti-ATP5A1 antibody NBP2-38525 (A) shows similar pattern to independent antibody NBP2-38470 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-38525] - Staining of human kidney.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-38525-img0007.jpg)
![Western Blot: ATP5A Antibody [NBP2-38525] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/ATP5A-Antibody-Western-Blot-NBP2-38525-img0002.jpg)
![Immunohistochemistry: ATP5A Antibody [NBP2-38525] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-NBP2-38525-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-38525] - Staining of human colon, heart muscle, kidney and liver using Anti-ATP5A1 antibody NBP2-38525 (A) shows similar protein distribution across tissues to independent antibody NBP2-38470 (B).](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-38525-img0003.jpg)
![Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-38525] - Staining of human liver.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-38525-img0005.jpg)
![Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-38525] - Staining of human heart muscle.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-38525-img0006.jpg)
![Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-38525] - Staining of human colon.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-38525-img0008.jpg)
![Western Blot: ATP5A Antibody [NBP2-38470] - Analysis using Anti-ATP5A1 antibody NBP2-38470 (A) shows similar pattern to independent antibody NBP2-38525 (B).](http://images.novusbio.com/fullsize/ATP5A-Antibody-Western-Blot-NBP2-38470-img0005.jpg "<b>Independent Antibodies Validation. </b>Western Blot: ATP5A Antibody [NBP2-38470] - Analysis using Anti-ATP5A1 antibody NBP2-38470 (A) shows similar pattern to independent antibody NBP2-38525 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-38470] - Staining of human colon.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-38470-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-38470] - Staining of human colon, heart muscle, kidney and liver using Anti-ATP5A1 antibody NBP2-38470 (A) shows similar protein distribution across tissues to independent antibody NBP2-38525 (B).](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-38470-img0004.jpg)
![Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-38470] - Staining of human liver.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-38470-img0006.jpg)
![Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-38470] - Staining of human kidney.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-38470-img0007.jpg)
![Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-38470] - Staining of human heart muscle.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-38470-img0009.jpg)
![Simple Western: ATP5A Antibody [NBP2-92928] - ATP5A Antibodies (NBP2-92928), ProteinSimple Western Blot on Jess Instrument. One microgram of human brain tissue lysate was tested with the antibodies diluted 1:10 or 1:20 times. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-92928_rabbit-polyclonal-atp5a-antibody-simple-western-2042023113534.jpg "Simple Western: ATP5A Antibody [NBP2-92928] - ATP5A Antibodies (NBP2-92928), ProteinSimple Western Blot on Jess Instrument. One microgram of human brain tissue lysate was tested with the antibodies diluted 1:10 or 1:20 times. Image from verified customer review.")
![Western Blot: ATP5A Antibody [NBP2-92928] - Analysis of extracts of various cell lines, using ATP5A at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Western-Blot-NBP2-92928-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: ATP5A Antibody - BSA Free [NBP2-92928] - Confocal immunofluorescence analysis of Hela cells using ATP5A Rabbit pAb at dilution of 1:200. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92928_rabbit-polyclonal-atp5a-antibody-4220251430450.jpg)
![Immunocytochemistry/ Immunofluorescence: ATP5A Antibody - BSA Free [NBP2-92928] - Confocal immunofluorescence analysis of U-2OS cells using ATP5A Rabbit pAb at dilution of 1:200. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92928_rabbit-polyclonal-atp5a-antibody-4220251429052.jpg)
![Western Blot: Jumonji/JARID2 Antibody (PSH01-32) [NBP3-32486] - Western blot analysis of Jumonji/JARID2 on different lysates with Rabbit anti-Jumonji/JARID2 antibody (NBP3-32486) at 1/1,000 dilution. Lane 1: NCCIT cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: MDA-MB-231 cell lysate (20 ug/Lane) Lane 6: A549 cell lysate (20 ug/Lane) Lane 7: HepG2 cell lysate (20 ug/Lane) Lane 8: HUVEC cell lysate (20 ug/Lane) Lane 9: HeLa cell lysate (20 ug/Lane) Lane 10: HEK-293 cell lysate (20 ug/Lane) Lane 11: Human brain tissue lysate (40 ug/Lane) Predicted band size: 139 kDa Observed band size: 139 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32486) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32486_rabbit-jumonji-jarid2-mab-psh01-32-278202416285253.jpg "Western Blot: Jumonji/JARID2 Antibody (PSH01-32) [NBP3-32486] - Western blot analysis of Jumonji/JARID2 on different lysates with Rabbit anti-Jumonji/JARID2 antibody (NBP3-32486) at 1/1,000 dilution. Lane 1: NCCIT cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: MDA-MB-231 cell lysate (20 ug/Lane) Lane 6: A549 cell lysate (20 ug/Lane) Lane 7: HepG2 cell lysate (20 ug/Lane) Lane 8: HUVEC cell lysate (20 ug/Lane) Lane 9: HeLa cell lysate (20 ug/Lane) Lane 10: HEK-293 cell lysate (20 ug/Lane) Lane 11: Human brain tissue lysate (40 ug/Lane) Predicted band size: 139 kDa Observed band size: 139 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32486) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Jumonji/JARID2 Antibody (PSH01-32) [NBP3-32486] - Immunohistochemical analysis of paraffin-embedded human cervix tissue with Rabbit anti-Jumonji/JARID2 antibody (NBP3-32486) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32486) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32486_rabbit-jumonji-jarid2-mab-psh01-32-298202414304155.jpg)
![Immunocytochemistry/ Immunofluorescence: Jumonji/JARID2 Antibody (PSH01-32) [NBP3-32486] - Immunocytochemistry analysis of NCCIT cells labeling Jumonji/JARID2 with Rabbit anti-Jumonji/JARID2 antibody (NBP3-32486) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Jumonji/JARID2 antibody (NBP3-32486) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32486_rabbit-jumonji-jarid2-mab-psh01-32-29202410484844.jpg)
![Immunohistochemistry;ATP5A Antibody[NBP3-29558]Detection of human ATP5A1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ATP5A1 used at a dilution of 1:5,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-29561_rabbit-atp5a-pab-78202493614.jpg "Immunohistochemistry;ATP5A Antibody[NBP3-29558]Detection of human ATP5A1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ATP5A1 used at a dilution of 1:5,000 . Detection: DAB")
![Immunohistochemistry;ATP5A Antibody[NBP3-29561]Detection of mouse ATP5A1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-ATP5A1 used at a dilution of 1:5,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-29561_rabbit-atp5a-pab-782024936119.jpg)
![Immunoprecipitation:ATP5A AntibodyNBP3-29561] -Detection of human ATP5A1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ATP5A1 antibody (NBP3-29561 lot 2) used for IP at 6 µg per reaction. ATP5A1 was also immunoprecipitated by a previous lot of this antibody (NBP3-29561 lot 1) and a second.inst a different epitope of ATP5A1 . For blotting immunoprecipitated ATP5A1, NBP3-29561 was used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/nbp3-29561_rabbit-atp5a-pab-301220241928131.jpg)
![Immunohistochemistry;ATP5A Antibody[NBP3-29711]Detection of mouse ATP5A1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-ATP5A1 used at a dilution of 1:5,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-29712_rabbit-atp5a-pab-78202410153527.jpg "Immunohistochemistry;ATP5A Antibody[NBP3-29711]Detection of mouse ATP5A1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-ATP5A1 used at a dilution of 1:5,000 . Detection: DAB")
![Immunohistochemistry;ATP5A Antibody[NBP3-29712]Detection of human ATP5A1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ATP5A1 used at a dilution of 1:5,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-29712_rabbit-atp5a-pab-782024936118.jpg)
![Immunoprecipitation:ATP5A AntibodyNBP3-29712] -Detection of human ATP5A1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ATP5A1 antibody NBP3-29712 (lot NBP3-29712-1) used for IP at 6 µg per reaction. ATP5A1 was also immunoprecipitated by rabbit anti-ATP5A1.. For blotting immunoprecipitated ATP5A1, NBP3-29712 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29712_rabbit-atp5a-pab-301220241633611.jpg)
![Western Blot: Glutamine Synthetase Antibody (GLUL/6600) [NBP3-13928] - Western blot analysis of Y79 cell lysate using Glutamine Synthetase antibody (GLUL/6600).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-GLUL-6600-Western-Blot-NBP3-13928-img0002.jpg "Western Blot: Glutamine Synthetase Antibody (GLUL/6600) [NBP3-13928] - Western blot analysis of Y79 cell lysate using Glutamine Synthetase antibody (GLUL/6600).")
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (GLUL/6600) [NBP3-13928] - Formalin-fixed, paraffin-embedded human stomachstained with Glutamine Synthetase antibody (GLUL/6600) at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-GLUL-6600-Immunohistochemistry-Paraffin-NBP3-13928-img0003.jpg)
![Western Blot: Glutamine Synthetase Antibody (GLUL/6600) [NBP3-13928] - Western blot analysis of human liver tissue lysate using Glutamine Synthetase antibody (GLUL/6600).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-GLUL-6600-Western-Blot-NBP3-13928-img0001.jpg)
![Immunohistochemistry: ATP5A Antibody (5G11) [NBP3-26491] - Image of ATP5A Antibody (5G11) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26491_rabbit-atp5a-mab-5g11-26220241422526.jpg "Immunohistochemistry: ATP5A Antibody (5G11) [NBP3-26491] - Image of ATP5A Antibody (5G11) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: ATP5A Antibody (5G11) [NBP3-26491] - Image of ATP5A Antibody (5G11) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26491_rabbit-atp5a-mab-5g11-262202415355739.jpg)
![Western Blot: ATP5A Antibody (5G11) [NBP3-26491] - Positive Western Blot detected in: HepG2 whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HL60 whole cell lysate, Mouse Heart tissue, Mouse Brain tissue. All lanes: ATP5A Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 60, 55, 58 kDa Observed band size: 60 kDa](http://images.novusbio.com/fullsize/nbp3-26491_rabbit-atp5a-mab-5g11-28220241144262.jpg)
![Western Blot: Glutamine Synthetase Antibody (A3G2-R) [NBP3-32387] - Western blot analysis of Glutamine Synthetase on different lysates with Mouse anti-Glutamine Synthetase antibody (NBP3-32387) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HepG2 cell lysate Lane 4: K-562 cell lysate Lane 5: Jurkat cell lysate Lane 6: HEK-293 cell lysate Lane 7: SK-Br-3 cell lysate Lane 8: Human liver tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32387) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32387_mouse-glutamine-synthetase-mab-a3g2-r-278202415242686.jpg "Western Blot: Glutamine Synthetase Antibody (A3G2-R) [NBP3-32387] - Western blot analysis of Glutamine Synthetase on different lysates with Mouse anti-Glutamine Synthetase antibody (NBP3-32387) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HepG2 cell lysate Lane 4: K-562 cell lysate Lane 5: Jurkat cell lysate Lane 6: HEK-293 cell lysate Lane 7: SK-Br-3 cell lysate Lane 8: Human liver tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32387) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Glutamine Synthetase Antibody (A3G2-R) [NBP3-32387] - Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-Glutamine Synthetase antibody (NBP3-32387) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32387) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32387_mouse-glutamine-synthetase-mab-a3g2-r-298202414333698.jpg)
![Western Blot: Glutamine Synthetase Antibody (GLUL/6604) [NBP3-13929] - Western blot analysis of human liver tissue using Glutamine Synthetase antibody (GLUL/6604).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-GLUL-6604-Western-Blot-NBP3-13929-img0002.jpg "Western Blot: Glutamine Synthetase Antibody (GLUL/6604) [NBP3-13929] - Western blot analysis of human liver tissue using Glutamine Synthetase antibody (GLUL/6604).")
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (GLUL/6604) [NBP3-13929] - Formalin-fixed, paraffin-embedded human liver stained with Glutamine Synthetase antibody (GLUL/6604) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-GLUL-6604-Immunohistochemistry-Paraffin-NBP3-13929-img0003.jpg)
![Western Blot: Glutamine Synthetase Antibody (GLUL/6604) [NBP3-13929] - Western blot analysis of Y79 cell lysate using Glutamine Synthetase antibody (GLUL/6604).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-GLUL-6604-Western-Blot-NBP3-13929-img0001.jpg)
