| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38021-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-17050-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-17032-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48805-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95146-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26322-50ul | |||
![Western Blot: EAAT2/GLT1 Antibody [NBP3-13348] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EAAT2/GLT1 antibody (NBP3-13348) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody-Western-Blot-NBP3-13348-img0001.jpg "Western Blot: EAAT2/GLT1 Antibody [NBP3-13348] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EAAT2/GLT1 antibody (NBP3-13348) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP3-13348] - EAAT2/GLT1 antibody detects EAAT2/GLT1 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse hippocampus. EAAT2/GLT1 stained by EAAT2/GLT1 antibody (NBP3-13348) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody-Immunohistochemistry-Paraffin-NBP3-13348-img0003.jpg)
![EAAT2 antibody detects EAAT2 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: EAAT2 stained by EAAT2 antibody (NBP3-13348) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a cytoplasm marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13348_rabbit-eaat2-glt1-pab-immunohistochemistry-paraffin-26112025182537.jpg)
![Immunohistochemistry: EAAT3 Antibody [NBP3-38021] - Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded mouse brain using SLC1A1 Rabbit pAb at dilution of 1:400 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38021_rabbit-eaat3-pab-10122024140439.jpg "Immunohistochemistry: EAAT3 Antibody [NBP3-38021] - Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded mouse brain using SLC1A1 Rabbit pAb at dilution of 1:400 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: EAAT3 Antibody [NBP3-38021] - Western blot analysis of various lysates using at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-38021_rabbit-eaat3-pab-10122024140454.jpg)
![Western Blot: EAAT2/GLT1 Antibody (PS01-62) [NBP3-32287] - Western blot analysis of EAAT2/GLT1 on different lysates with Rabbit anti-EAAT2/GLT1 antibody (NBP3-32287) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate (20 ug/Lane) Lane 2: Rat brain tissue lysate (20 ug/Lane) Lane 3: Mouse cerebellum tissue lysate (20 ug/Lane) Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32287) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32287_rabbit-eaat2-glt1-mab-ps01-62-278202415242637.jpg "Western Blot: EAAT2/GLT1 Antibody (PS01-62) [NBP3-32287] - Western blot analysis of EAAT2/GLT1 on different lysates with Rabbit anti-EAAT2/GLT1 antibody (NBP3-32287) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate (20 ug/Lane) Lane 2: Rat brain tissue lysate (20 ug/Lane) Lane 3: Mouse cerebellum tissue lysate (20 ug/Lane) Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32287) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: EAAT2/GLT1 Antibody (PS01-62) [NBP3-32287] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-EAAT2/GLT1 antibody (NBP3-32287) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32287) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32287_rabbit-eaat2-glt1-mab-ps01-62-29820241435275.jpg)
![Immunocytochemistry/Immunofluorescence: EAAT3 Antibody [NBP3-17050] - Analysis in human small intestine and testis tissues using Anti-SLC1A1 antibody. Corresponding SLC1A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/EAAT3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-17050-img0001.jpg "Immunocytochemistry/Immunofluorescence: EAAT3 Antibody [NBP3-17050] - Analysis in human small intestine and testis tissues using Anti-SLC1A1 antibody. Corresponding SLC1A1 RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: EAAT3 Antibody [NBP3-17050] - Staining of human small intestine shows high expression.](http://images.novusbio.com/fullsize/EAAT3-Antibody-Immunohistochemistry-Paraffin-NBP3-17050-img0002.jpg)
![Immunohistochemistry-Paraffin: EAAT3 Antibody [NBP3-17050] - Staining of human testis shows low expression as expected.](http://images.novusbio.com/fullsize/EAAT3-Antibody-Immunohistochemistry-Paraffin-NBP3-17050-img0003.jpg)
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP3-17032] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody-Immunohistochemistry-Paraffin-NBP3-17032-img0002.jpg "Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP3-17032] - Staining of human cerebral cortex shows high expression.")
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP3-17032] - Analysis in human cerebral cortex and small intestine tissues using Anti-SLC1A2 antibody. Corresponding SLC1A2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody-Immunohistochemistry-Paraffin-NBP3-17032-img0001.jpg)
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP3-17032] - Staining of human small intestine shows low expression as expected.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody-Immunohistochemistry-Paraffin-NBP3-17032-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: EAAT4 Antibody [NBP2-48805] - Staining of human cell line U-2 OS shows localization to intermediate filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/EAAT4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48805-img0002.jpg "Immunocytochemistry/Immunofluorescence: EAAT4 Antibody [NBP2-48805] - Staining of human cell line U-2 OS shows localization to intermediate filaments. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: EAAT4 Antibody [NBP2-48805] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/EAAT4-Antibody-Immunohistochemistry-NBP2-48805-img0001.jpg)
![Western Blot: EAAT2/GLT1 Antibody [NBP1-59633] - HT1080 cell lysate, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody-Western-Blot-NBP1-59633-img0001.jpg "Western Blot: EAAT2/GLT1 Antibody [NBP1-59633] - HT1080 cell lysate, Antibody Titration: 0.2-1 ug/ml")
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP1-59633] - Human Brain, cortex tissue at an antibody concentration of 5 ug/ml.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody-Immunohistochemistry-Paraffin-NBP1-59633-img0003.jpg)
![Immunoprecipitation: xCT Antibody (JE31-93) [NBP3-33014] - xCT was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-33014 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33014 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-33014 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-33014 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds; ECL](http://images.novusbio.com/fullsize/nbp3-33014_rabbit-xct-mab-je31-93-26820241582617.jpg "Immunoprecipitation: xCT Antibody (JE31-93) [NBP3-33014] - xCT was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-33014 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33014 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-33014 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-33014 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds; ECL")
![Western Blot: xCT Antibody (JE31-93) [NBP3-33014] - Western blot analysis of xCT on different lysates with Rabbit anti-xCT antibody (NBP3-33014) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: 293T cell lysate (20 ug/Lane) Lane 4: Mouse liver tissue lysate (40 ug/Lane) Lane 5: Mouse brain tissue lysate (40 ug/Lane) Lane 6: Rat brain tissue lysate (40 ug/Lane) Lane 7: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33014) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33014_rabbit-xct-mab-je31-93-288202412493489.jpg)
![Western Blot: xCT Antibody - Azide and BSA Free [xCT] - Western blot analysis of lysates from Rat brain using xCT Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp2-95146_rabbit-polyclonal-xct-antibody-5220251628035.jpg "Western Blot: xCT Antibody - Azide and BSA Free [xCT] - Western blot analysis of lysates from Rat brain using xCT Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Western Blot: EAAT2/GLT1 Antibody (28F9) [NBP3-26322] - Positive Western Blot detected in: U251 whole cell lysate. All lanes: EAAT2/GLT1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 62 kDa Observed band size: 62 kDa](http://images.novusbio.com/fullsize/nbp3-26322_rabbit-eaat2-glt1-mab-28f9-28220241140946.jpg "Western Blot: EAAT2/GLT1 Antibody (28F9) [NBP3-26322] - Positive Western Blot detected in: U251 whole cell lysate. All lanes: EAAT2/GLT1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 62 kDa Observed band size: 62 kDa")
![Flow Cytometry: EAAT2/GLT1 Antibody (28F9) [NBP3-26322] - Overlay Peak curve showing MCF-7 cells stained with EAAT2/GLT1 Antibody (28F9) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26322_rabbit-eaat2-glt1-mab-28f9-282202414183678.jpg)