| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77179-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74910 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-24559-25ul | |||
| Azide and BSA Free | NBP3-43869 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-32352-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03009-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37329-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13159SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54523-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44411 | |||
| BSA Free | NBP2-42385-25ul | |||
![Western Blot: N-Cadherin Antibody [NBP2-19460] - Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Western-Blot-NBP2-19460-img0012.jpg "<b>Genetic Strategies Validation. </b>Western Blot: N-Cadherin Antibody [NBP2-19460] - Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: N Cadherin Antibody [NBP2-19460] - Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-19460-img0002.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19460] - Paraffin-embedded hepatoma. N-Cadherin antibody [N2C1], Internal dilution: 1:500.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-19460-img0003.jpg)
![Western Blot: N-Cadherin Antibody [NBP2-19460] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (NBP2-19460) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19460_rabbit-polyclonal-n-cadherin-antibody-91020242014643.jpg)
![N-Cadherin antibody [N2C1], Internal detects N-Cadherin protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: N-Cadherin protein stained by N-Cadherin antibody [N2C1], Internal (NBP2-19460) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-19460_rabbit-polyclonal-n-cadherin-antibody-immunohistochemistry-frozen-261120251842318.jpg)
![Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (NBP2-19460) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-19460_rabbit-polyclonal-n-cadherin-antibody-western-blot-261120251828100.jpg)
![Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (NBP2-19460) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-19460_rabbit-polyclonal-n-cadherin-antibody-western-blot-261120251846100.jpg)
![Western Blot: ZEB2 Antibody [NBP1-77179] - Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20uM of MG132 for 4 h before harvested. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0014.jpg "<b>Biological Strategies Validation. </b>Western Blot: ZEB2 Antibody [NBP1-77179] - Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20uM of MG132 for 4 h before harvested. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: ZEB2 Antibody [NBP1-77179] - Analysis in EL4 cell lysate with antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: ZEB2 Antibody - BSA Free [NBP1-77179] - Immunofluorescence of ZEB2 in Jurkat cells with ZEB2 antibody at 20 ug/ml.Red: ZEB2 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-2711202419333477.gif)
![Western Blot: ZEB2 Antibody [NBP1-77179] - Lysates from 293T cells transfected Flag-Zeb2 with or without HA-Fbxo45 were immunoprecipitated (IP) with anti-Flag M2 antibody, and then immunoblotted. Total lysates were also used as Input for immunoblotting analysis. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0012.jpg)
![Western Blot: ZEB2 Antibody [NBP1-77179] - The protein levels of EMT-TFs were compared between carcinoma cell lines and non- or less-malignant tumor cell lines by using Western-blot analysis. The soft agar colony transformation or migration assay was referred to distinguish malignant degree of the experimental cancer cell lines (data not shown). Malignant degree: prostate cancer cell lines, P69 < M12; breast cancer cell lines, MCF7 < MD-MBA-231; lung cancer cell lines, A549 < H1299; liver cancer cell lines, HepG2 < SMMC7721. 'kD' (kilo-dalton) means the molecular weight of protein. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0013.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202415392525.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202415334920.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202415395947.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-31020241535644.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Functional domains of Fbxo45 for ubiquitination of Zeb2A. Lysates from 293T cells transfected Flag-Zeb2 with or without HA-Fbxo45 were immunoprecipitated (IP) with anti-Flag M2 antibody, & then immunoblotted. Total lysates were also used as Input for immunoblotting analysis. B. Bacterially expressed GST or GST–Fbxo45, -Fbxo45 delta F-box, -Fbxo45 delta mid, or -Fbxo45 delta SPRY fusion proteins & Glutathione-Sepharose beads were incubated with lysates of HeLa cells transfected with Zeb2, Snai1 or Snai2, respectively. The proteins associated with GST–tagged Fbxo45 forms, bound on the Glutathione-Sepharose beads were washed five times with the RIPA buffer before immunoblotting. C. Lysates from 293T cells transfected Flag-Zeb2 with HA-Fbxo45, -Fbxo45 delta mid, -Fbxo45 delta F-box or -Fbxo45 delta SPRY were immunoprecipitated with anti-Flag or anti-HA antibodies, & immunoprecipitates were resolved by SDS-PAGE for Western-blot analysis. D. U2OS cells transfected with Flag-Zeb2 & HA-Fbxo45, -Fbxo45 delta F-box or -Fbxo45 delta SPRY were stained using the primary antibodies of anti-Flag M2 or anti-HA, & the second antibodies of Alexa Fluor 568 anti-mouse or Alexa Fluor 488 anti-Rabbit, respectively. Scale: 25μm. E-I. Zeb2 ubiquitination assays by using IP expriments under different conditions: Flag-Zeb2 with or without HA-Ub (E); Flag-Zeb2 with HA-Ub WT, K48R or K63R (F); Flag-Zeb2 & HA-Ub K48-only with or without myc-Fbox45 (G); Flag-Zeb2 & HA-Ub K48-only with siRNA control, siRNAs for Fbxo45 or siRNAs for Pam (H); Flag-Zeb2 & HA-Ub with HA-Fbxo45, -Fbxo45 delta F-box or -Fbxo45 delta SPRY (I). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202416163752.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 is a direct target of miR-27a* that mediates EMT processesA, C. The expression levels of miR-27a* (A) & Fbxo45 mRNA (C) in P69 & M12 were shown according to the data of miRNA transcriptome sequencing & DGE sequencing, respectively [46]. TPM means transcripts per million. B. Real-time PCR in delta delta Ct methods were used to confirm the expression levels of miR-27a* in P69 & M12. One representative experiment out of three was shown. D. Fbxo45 mRNA levels in stable cell lines miR-27a*-overexpressing P69 & miR-27a*-silencing M12 (zipmiR-27a*). P69 or M12 stably infected with the same lentiviral empty vector was used as a control (ctrl). E. Fbxo45 3′-UTR wide-type or mutant form at the position where is complementary to the 5′ seed region of miR-27a* was subcloned into psiCheck2 vector. 293T cells were transfected with psiCheck2 containing WT or mutant Fbxo45 3′-UTR & miR-27a* or non-specific control vector. The Renilla luciferase activity was normalized on the constitutive activity of firefly luciferase. Data are the mean±S.E.M. of three independent experiments. F-G. Immunoblot for Fbxo45, core EMT-TFs (Zeb1, Zeb2, Twist1) & their associated EMT markers (Fibronectin, N-cadherin, Laminin C2, Vimentin & E-cadherin) in stable cell lines P69-miR-27a* or M12-zipmiR-27a* (F); Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20μM of MG132 for 4 h before harvested (G). GAPDH is for a loading control. H. Immunblot for Fbxo45, N-cadherin, E-cadherin & five core EMT-TFs in stable cell lines PC3-ctrl, PC3-miR-27a* & PC3-zipmiR-27a*. alpha -tubulin is for a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202416175234.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 is a direct target of miR-27a* that mediates EMT processesA, C. The expression levels of miR-27a* (A) & Fbxo45 mRNA (C) in P69 & M12 were shown according to the data of miRNA transcriptome sequencing & DGE sequencing, respectively [46]. TPM means transcripts per million. B. Real-time PCR in delta delta Ct methods were used to confirm the expression levels of miR-27a* in P69 & M12. One representative experiment out of three was shown. D. Fbxo45 mRNA levels in stable cell lines miR-27a*-overexpressing P69 & miR-27a*-silencing M12 (zipmiR-27a*). P69 or M12 stably infected with the same lentiviral empty vector was used as a control (ctrl). E. Fbxo45 3′-UTR wide-type or mutant form at the position where is complementary to the 5′ seed region of miR-27a* was subcloned into psiCheck2 vector. 293T cells were transfected with psiCheck2 containing WT or mutant Fbxo45 3′-UTR & miR-27a* or non-specific control vector. The Renilla luciferase activity was normalized on the constitutive activity of firefly luciferase. Data are the mean±S.E.M. of three independent experiments. F-G. Immunoblot for Fbxo45, core EMT-TFs (Zeb1, Zeb2, Twist1) & their associated EMT markers (Fibronectin, N-cadherin, Laminin C2, Vimentin & E-cadherin) in stable cell lines P69-miR-27a* or M12-zipmiR-27a* (F); Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20μM of MG132 for 4 h before harvested (G). GAPDH is for a loading control. H. Immunblot for Fbxo45, N-cadherin, E-cadherin & five core EMT-TFs in stable cell lines PC3-ctrl, PC3-miR-27a* & PC3-zipmiR-27a*. alpha -tubulin is for a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-31020241616011.jpg)
![Immunocytochemistry/ Immunofluorescence: ZEB2 Antibody - BSA Free [NBP1-77179] - Immunocytochemistry of ZEB2 in Jurkat cells with ZEB2 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-2711202420285893.jpg)
![Immunocytochemistry/ Immunofluorescence: ZEB2 Antibody - BSA Free [NBP1-77179] - Immunofluorescence of ZEB2 in Jurkat cells with ZEB2 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-2711202420272496.jpg)
![Western Blot: ZEB1 Antibody (3G6) [NBP2-46398] - Cross-reactivity test with ZEB2: Analysis of HEK293T cells were transfected with plasmids overexpressing either ZEB2 (left) or ZEB1 (right).](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Western-Blot-NBP2-46398-img0007.jpg "Western Blot: ZEB1 Antibody (3G6) [NBP2-46398] - Cross-reactivity test with ZEB2: Analysis of HEK293T cells were transfected with plasmids overexpressing either ZEB2 (left) or ZEB1 (right).")
![Immunohistochemistry: ZEB1 Antibody (3G6) [NBP2-46398] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Immunohistochemistry-NBP2-46398-img0006.jpg)
![Western Blot: ZEB1 Antibody (3G6) [NBP2-46398] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Western-Blot-NBP2-46398-img0008.jpg)
![Immunohistochemistry: ZEB1 Antibody (3G6) [NBP2-46398] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Immunohistochemistry-NBP2-46398-img0001.jpg)
![Immunohistochemistry: ZEB1 Antibody (3G6) [NBP2-46398] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Immunohistochemistry-NBP2-46398-img0002.jpg)
![Immunohistochemistry: ZEB1 Antibody (3G6) [NBP2-46398] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Immunohistochemistry-NBP2-46398-img0003.jpg)
![Immunohistochemistry: ZEB1 Antibody (3G6) [NBP2-46398] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Immunohistochemistry-NBP2-46398-img0004.jpg)
![Immunohistochemistry: ZEB1 Antibody (3G6) [NBP2-46398] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Immunohistochemistry-NBP2-46398-img0005.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody (5D5) [NBP1-51612] - Analysis of human lung cancer (A), colon cancer (B), ovarian cancer (C) and mammary cancer(D), using CDH2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-5D5-Immunohistochemistry-Paraffin-NBP1-51612-img0015.jpg "Immunohistochemistry-Paraffin: N-Cadherin Antibody (5D5) [NBP1-51612] - Analysis of human lung cancer (A), colon cancer (B), ovarian cancer (C) and mammary cancer(D), using CDH2 mouse mAb with DAB staining.")
![Western Blot: N-Cadherin Antibody (5D5) [NBP1-51612] - Analysis using CDH2 mouse mAb against A431 (1), NIH/3T3 (2), Hela (3), C6 (4) and LNCap (5) cell lysate.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-5D5-Western-Blot-NBP1-51612-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (5D5) [NBP1-51612] - Immunofluorescence analysis of A431 cells using CDH2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-5D5-Immunocytochemistry-Immunofluorescence-NBP1-51612-img0012.jpg)
![Flow Cytometry: N-Cadherin Antibody (5D5) [NBP1-51612] - Analysis of PC-2 cells using CDH2 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-5D5-Flow-Cytometry-NBP1-51612-img0011.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody (5D5) [NBP1-51612] - Analysis of human Placenta tissues using CDH2 mouse mAb](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-5D5-Immunohistochemistry-Paraffin-NBP1-51612-img0014.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP2-32352] - Analysis in human placenta and prostate tissues. Corresponding LIN-28B RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP2-32352-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP2-32352] - Analysis in human placenta and prostate tissues. Corresponding LIN-28B RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP2-32352] - Staining of human kidney, liver, placenta and prostate using Anti-LIN-28B antibody NBP2-32352 (A) shows similar protein distribution across tissues to independent antibody NBP1-85438 (B).](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP2-32352-img0015.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: LIN-28B Antibody [NBP2-32352] - Analysis in human cell line HEK 293 and human cell line MCF-7.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Western-Blot-NBP2-32352-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: LIN-28B Antibody [NBP2-32352] - Immunofluorescent staining of human cell line HEK 293 shows localization to nucleus, nucleoli & cytosol.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-32352-img0004.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP2-32352] - Staining of human prostate shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP2-32352-img0019.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP2-32352] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP2-32352-img0016.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP2-32352] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP2-32352-img0017.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP2-32352] - Staining of human placenta shows moderate positivity in endothelial cells.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP2-32352-img0018.jpg)
![Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249422625.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - ZEB1 antibody [HL2245] detects ZEB1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZEB1 stained by ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249374983.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - ZEB1 antibody [HL2245] detects ZEB1 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. ZEB1 stained by ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249374923.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249422673.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249393552.jpg)
![Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-212024941465.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - ZEB1 antibody [HL2245] detects ZEB1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. ZEB1 stained by ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249415991.jpg)
![Western Blot: N-Cadherin Antibody - Azide and BSA Free [N-Cadherin] - Western blot analysis of lysates from Mouse heart using N-Cadherin Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time:30s.](http://images.novusbio.com/fullsize/nbp3-03009_rabbit-polyclonal-n-cadherin-antibody-52202516334171.jpg "Western Blot: N-Cadherin Antibody - Azide and BSA Free [N-Cadherin] - Western blot analysis of lysates from Mouse heart using N-Cadherin Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time:30s.")
![Immunocytochemistry/ Immunofluorescence: N-Cadherin Antibody - Azide and BSA Free [N-Cadherin] - Immunofluorescence analysis of paraffin-embedded mouse heart using N-Cadherin Rabbit pAb . Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03009_rabbit-polyclonal-n-cadherin-antibody-52202516334199.jpg)
![Immunocytochemistry/ Immunofluorescence: N-Cadherin Antibody - Azide and BSA Free [N-Cadherin] - Immunofluorescence analysis of paraffin-embedded rat heart using N-Cadherin Rabbit pAb . Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03009_rabbit-polyclonal-n-cadherin-antibody-5220251639652.jpg)
![Immunohistochemistry: N-Cadherin Antibody - Azide and BSA Free [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using N-Cadherin Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03009_rabbit-polyclonal-n-cadherin-antibody-52202516283288.jpg)
![Immunohistochemistry: N-Cadherin Antibody - Azide and BSA Free [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using N-Cadherin Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03009_rabbit-polyclonal-n-cadherin-antibody-52202516334195.jpg)
![Immunohistochemistry: N-Cadherin Antibody - Azide and BSA Free [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using N-Cadherin Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03009_rabbit-polyclonal-n-cadherin-antibody-52202516293959.jpg)
![Immunohistochemistry: N-Cadherin Antibody - Azide and BSA Free [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using N-Cadherin Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03009_rabbit-polyclonal-n-cadherin-antibody-52202516321447.jpg)
![Western Blot: ZEB1 Antibody (2A8H3) [NBP2-37329] - Western blot analysis using ZEB1 mAb against HEK293 (1) and ZEB1 (AA: 967-1108)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Western-Blot-NBP2-37329-img0003.jpg "Western Blot: ZEB1 Antibody (2A8H3) [NBP2-37329] - Western blot analysis using ZEB1 mAb against HEK293 (1) and ZEB1 (AA: 967-1108)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (2A8H3) [NBP2-37329] - Immunofluorescence analysis of Hela cells using ZEB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Immunocytochemistry-Immunofluorescence-NBP2-37329-img0004.jpg)
![Immunohistochemistry: ZEB1 Antibody (2A8H3) [NBP2-37329] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ZEB1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Immunohistochemistry-NBP2-37329-img0007.jpg)
![Flow Cytometry: ZEB1 Antibody (2A8H3) [NBP2-37329] - Flow cytometric analysis of Hela cells using ZEB1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Flow-Cytometry-NBP2-37329-img0005.jpg)
![Western Blot: ZEB1 Antibody (2A8H3) [NBP2-37329] - Western blot analysis using ZEB1 mAb against human ZEB1 recombinant protein. (Expected MW is 41.7 kDa)](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Western-Blot-NBP2-37329-img0002.jpg)
![Immunohistochemistry: ZEB1 Antibody (2A8H3) [NBP2-37329] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ZEB1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Immunohistochemistry-NBP2-37329-img0006.jpg)
![ELISA: ZEB1 Antibody (2A8H3) [NBP2-37329] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-ELISA-NBP2-37329-img0001.jpg)
![Western Blot: ZEB1 Antibody [NBP2-13159] - Analysis of Zeb1 in (1) HeLa (2) Cos7 (3) HepG2 and (4) Ntera2 cell lysates.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Western-Blot-NBP2-13159-img0002.jpg "Western Blot: ZEB1 Antibody [NBP2-13159] - Analysis of Zeb1 in (1) HeLa (2) Cos7 (3) HepG2 and (4) Ntera2 cell lysates.")
![Immunocytochemistry/Immunofluorescence: ZEB1 Antibody [NBP2-13159] - ZEB1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunocytochemistry-NBP2-13159-img0001.jpg)
![Simple Western: ZEB1 Antibody [NBP2-13159] - Lane view shows a specific band for ZEB1 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Simple-Western-NBP2-13159-img0003.jpg)
![Western Blot: N-Cadherin Antibody (8C11) [NBP2-53321] - Western blot analysis of Heart tissue lysate using N-Cadherin antibody (8C11).](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-8C11-Western-Blot-NBP2-53321-img0002.jpg "Western Blot: N-Cadherin Antibody (8C11) [NBP2-53321] - Western blot analysis of Heart tissue lysate using N-Cadherin antibody (8C11).")
![Immunohistochemistry-Paraffin: N-Cadherin Antibody (8C11) [NBP2-53321] - Mouse heart stained with N-Cadherein antibody (8C11).](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-8C11-Immunohistochemistry-Paraffin-NBP2-53321-img0001.jpg)
![Western Blot: Laminin beta 1 Antibody (CL2970) [NBP2-42385] - Analysis of purified human recombinant Laminin-111, Laminin-211, Laminin-411, Laminin-511, Laminin-521 and Laminin-332.](http://images.novusbio.com/fullsize/Laminin-beta-1-Antibody-CL2970-Western-Blot-NBP2-42385-img0016.jpg "Western Blot: Laminin beta 1 Antibody (CL2970) [NBP2-42385] - Analysis of purified human recombinant Laminin-111, Laminin-211, Laminin-411, Laminin-511, Laminin-521 and Laminin-332.")
![Immunohistochemistry-Paraffin: Laminin beta 1 Antibody (CL2970) [NBP2-42385] - Staining of human lymph node shows absence of immunoreactivity in lymphoid cells (negative control).](http://images.novusbio.com/fullsize/Laminin-beta-1-Antibody-CL2970-Immunohistochemistry-Paraffin-NBP2-42385-img0013.jpg)
![Western Blot: Laminin beta 1 Antibody (CL2970) [NBP2-42385] - Analysis in human placenta tissue.](http://images.novusbio.com/fullsize/Laminin-beta-1-Antibody-CL2970-Western-Blot-NBP2-42385-img0014.jpg)
![Immunohistochemistry-Paraffin: Laminin beta 1 Antibody (CL2970) [NBP2-42385] - Staining of human placenta shows moderate immunoreactivity in basement membrane of trophoblast and in endothelium.](http://images.novusbio.com/fullsize/Laminin-beta-1-Antibody-CL2970-Immunohistochemistry-Paraffin-NBP2-42385-img0009.jpg)
![Immunohistochemistry-Paraffin: Laminin beta 1 Antibody (CL2970) [NBP2-42385] - Staining of human pancreas shows moderate positivity in basement membrane of exocrine glands.](http://images.novusbio.com/fullsize/Laminin-beta-1-Antibody-CL2970-Immunohistochemistry-Paraffin-NBP2-42385-img0010.jpg)
![Immunohistochemistry-Paraffin: Laminin beta 1 Antibody (CL2970) [NBP2-42385] - Staining of human endometrium shows immunoreactivity in basement membrane of glandular epithelium.](http://images.novusbio.com/fullsize/Laminin-beta-1-Antibody-CL2970-Immunohistochemistry-Paraffin-NBP2-42385-img0011.jpg)
![Immunohistochemistry-Paraffin: Laminin beta 1 Antibody (CL2970) [NBP2-42385] - Staining of human stomach shows positivity in basement membrane of glandular epithelium.](http://images.novusbio.com/fullsize/Laminin-beta-1-Antibody-CL2970-Immunohistochemistry-Paraffin-NBP2-42385-img0012.jpg)