| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26441-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26699-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88961-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26247-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81060-25ul | |||
![Immunohistochemistry: Pannexin-1 Antibody (28G5) [NBP3-26441] - Image of Pannexin-1 Antibody (28G5) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.1:50% DAB.](http://images.novusbio.com/fullsize/nbp3-26441_rabbit-pannexin-1-mab-28g5-262202414225215.jpg "Immunohistochemistry: Pannexin-1 Antibody (28G5) [NBP3-26441] - Image of Pannexin-1 Antibody (28G5) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.1:50% DAB.")
![Immunohistochemistry: Pannexin-1 Antibody (28G5) [NBP3-26441] - Image of Pannexin-1 Antibody (28G5) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.1:50% DAB.](http://images.novusbio.com/fullsize/nbp3-26441_rabbit-pannexin-1-mab-28g5-2722024847192.jpg)
![Immunocytochemistry/Immunofluorescence: Pannexin-1 Antibody (28G5) [NBP3-26441] - Staining of U251 with Pannexin-1 Antibody (28G5) at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 502-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26441_rabbit-pannexin-1-mab-28g5-282202412493632.jpg)
![Flow Cytometry: Pannexin-1 Antibody (28G5) [NBP3-26441] - Overlay Peak curve showing A549 cells stained with Pannexin-1 Antibody (28G5) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26441_rabbit-pannexin-1-mab-28g5-282202414312766.jpg)
![Western Blot: Gemin 3 Antibody [NB100-1801] - Detection of Human DDX20 on HeLa whole cell lystate using NB100-1801. DDX20 was also immunoprecipitated using rabbit anti-DDX20 antibody NB100-1802 and two.](http://images.novusbio.com/fullsize/Gemin-3-Antibody-Western-Blot-NB100-1801-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Gemin 3 Antibody [NB100-1801] - Detection of Human DDX20 on HeLa whole cell lystate using NB100-1801. DDX20 was also immunoprecipitated using rabbit anti-DDX20 antibody NB100-1802 and two.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: Gemin 3 Antibody [NB100-1801] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DDX20 used at a dilution of 1:200 ( 1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Gemin-3-Antibody-Immunohistochemistry-NB100-1801-img0003.jpg)
![Western Blot: ATP5D Antibody [NBP1-32365] - Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/ATP5D-Antibody-Western-Blot-NBP1-32365-img0005.jpg "Western Blot: ATP5D Antibody [NBP1-32365] - Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: ATP5D Antibody [NBP1-32365] - Paraffin-embedded breast cancer. ATP synthase delta antibody dilution: 1:250.](http://images.novusbio.com/fullsize/ATP5D-Antibody-Immunohistochemistry-Paraffin-NBP1-32365-img0006.jpg)
![Western Blot: ATP5D Antibody [NBP1-32365] -HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with ATP5D antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-32365_rabbit-polyclonal-atp5d-antibody-western-blot-8220249370..jpg)
![Western Blot: WWOX Antibody [NBP2-32122] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-WWOX antibody NBP2-32122 used for IP at 6 ug/mg lysate. WWOX was also immunoprecipitated by rabbit anti-WWOX antibody. For blotting immunoprecipitated WWOX, NBP2-32122 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/WWOX-Antibody-Western-Blot-NBP2-32122-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: WWOX Antibody [NBP2-32122] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-WWOX antibody NBP2-32122 used for IP at 6 ug/mg lysate. WWOX was also immunoprecipitated by rabbit anti-WWOX antibody. For blotting immunoprecipitated WWOX, NBP2-32122 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: WWOX Antibody [NBP2-32122] - Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- WWOX used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-NBP2-32122-img0003.jpg)
![Immunohistochemistry: WWOX Antibody [NBP2-32122] - Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- WWOX used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-NBP2-32122-img0002.jpg)
![Immunohistochemistry: Caspase-3 Antibody (5B2) - Pro [NBP3-26699] - Image of Caspase-3 Antibody (5B2) - Pro diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26699_rabbit-caspase-3-mab-5b2-pro-262202415475638.jpg "Immunohistochemistry: Caspase-3 Antibody (5B2) - Pro [NBP3-26699] - Image of Caspase-3 Antibody (5B2) - Pro diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: Caspase-3 Antibody (5B2) - Pro [NBP3-26699] - Positive Western Blot detected in: Hela whole cell lysate, HepG2 whole cell lysate, HEK293 whole cell lysate, Ntera-2 whole cell lysate. All lanes: Caspase-3 Antibody - Pro at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 32 kDa Observed band size: 32 kDa](http://images.novusbio.com/fullsize/nbp3-26699_rabbit-caspase-3-mab-5b2-pro-28220241140943.jpg)
![Immunocytochemistry/Immunofluorescence: Caspase-3 Antibody (5B2) - Pro [NBP3-26699] - Staining of Hela Cells with Caspase-3 Antibody (5B2) - Pro at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26699_rabbit-caspase-3-mab-5b2-pro-2822024132361.jpg)
![Immunocytochemistry/ Immunofluorescence: P2Y2 Antibody [NBP2-15060] - P2Y2 antibody [N3C3] detects P2Y2 protein at cell membrane by immunofluorescent analysis.Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: P2Y2 stained by P2Y2 antibody [N3C3] (NBP2-15060) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-15060_rabbit-polyclonal-p2y2-antibody-91020242011451.jpg "Immunocytochemistry/ Immunofluorescence: P2Y2 Antibody [NBP2-15060] - P2Y2 antibody [N3C3] detects P2Y2 protein at cell membrane by immunofluorescent analysis.Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: P2Y2 stained by P2Y2 antibody [N3C3] (NBP2-15060) diluted at 1:500.Blue: Hoechst 33342 staining.")
![Western Blot: P2Y2 Antibody [NBP2-15060] - Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [N3C3] (NBP2-15060) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15060_rabbit-polyclonal-p2y2-antibody-91020242001015.jpg)
![Boiled and unboiled HL-60 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [N3C3] (NBP2-15060) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)](http://images.novusbio.com/fullsize/nbp2-15060_rabbit-polyclonal-p2y2-antibody-western-blot-261120251827013.jpg)
![Western Blot: PPP2R5C Antibody [NBP1-88961] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/PPP2R5C-Antibody-Western-Blot-NBP1-88961-img0005.jpg "Western Blot: PPP2R5C Antibody [NBP1-88961] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunohistochemistry-Paraffin: PPP2R5C Antibody [NBP1-88961] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize/PPP2R5C-Antibody-Immunohistochemistry-Paraffin-NBP1-88961-img0002.jpg)
![Western Blot: CPT1A Antibody [NBP2-15993] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CPT1A antibody [C1C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CPT1A-Antibody-Western-Blot-NBP2-15993-img0004.jpg "Western Blot: CPT1A Antibody [NBP2-15993] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CPT1A antibody [C1C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: CPT1A Antibody [NBP2-15993] - Mouse kidney. CPT1A stained by CPT1A antibody [C1C2], Internal diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/CPT1A-Antibody-Immunohistochemistry-Paraffin-NBP2-15993-img0005.jpg)
![Immunohistochemistry-Paraffin: CPT1A Antibody [NBP2-15993] - MDA-MB157 xenograft, using CPT1A antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/CPT1A-Antibody-Immunohistochemistry-Paraffin-NBP2-15993-img0002.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody (6F6) [NBP3-26247] - Positive Western Blot detected in: U87 whole cell lysate, MCF-7 whole cell lysate, PC3 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate, THP-1 whole cell lysate, A549 whole cell lysate, Mouse brain tissue, Rat brain tissue. All lanes: 14-3-3 beta/alpha Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 29, 28 kDa Observed band size: 25-35 kDa](http://images.novusbio.com/fullsize/nbp3-26247_rabbit-14-3-3-beta-alpha-mab-6f6-282202411351616.jpg "Western Blot: 14-3-3 beta/alpha Antibody (6F6) [NBP3-26247] - Positive Western Blot detected in: U87 whole cell lysate, MCF-7 whole cell lysate, PC3 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate, THP-1 whole cell lysate, A549 whole cell lysate, Mouse brain tissue, Rat brain tissue. All lanes: 14-3-3 beta/alpha Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 29, 28 kDa Observed band size: 25-35 kDa")
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody (6F6) [NBP3-26247] - Staining of PC-3 cell with 14-3-3 beta/alpha Antibody (6F6) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 494-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26247_rabbit-14-3-3-beta-alpha-mab-6f6-282202412493639.jpg)
![Flow Cytometry: 14-3-3 beta/alpha Antibody (6F6) [NBP3-26247] - Overlay Peak curve showing Hela cells stained with 14-3-3 beta/alpha Antibody (6F6) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26247_rabbit-14-3-3-beta-alpha-mab-6f6-282202414183655.jpg)
![Immunohistochemistry-Paraffin: PRAS40 Antibody [NBP2-19924] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PRAS40-Antibody-Immunohistochemistry-Paraffin-NBP2-19924-img0003.jpg "Immunohistochemistry-Paraffin: PRAS40 Antibody [NBP2-19924] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.")
![Immunocytochemistry/Immunofluorescence: AHNAK Antibody [NBP1-81060] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.](http://images.novusbio.com/fullsize/AHNAK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81060-img0008.jpg "Immunocytochemistry/Immunofluorescence: AHNAK Antibody [NBP1-81060] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.")
![Immunohistochemistry-Paraffin: AHNAK Antibody [NBP1-81060] - Staining of human oral mucosa shows cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/AHNAK-Antibody-Immunohistochemistry-Paraffin-NBP1-81060-img0007.jpg)