| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97616-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89294-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98964-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89210-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06496-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97806-50ul | |||
![Western Blot: CPT1A Antibody [NBP1-59608] - Lanes: 1. 45ug capan1 cell lysate 2. 45 ug HPAF cell lysate Primary, Antibody Dilution: 1 : 1000 Secondary Antibody: Anti-Rabbit HRP Secondary, Antibody Dilution: 1 : 5000 Gene name: CPT1A.](http://images.novusbio.com/fullsize/CPT1A-Antibody-Western-Blot-NBP1-59608-img0006.jpg "Western Blot: CPT1A Antibody [NBP1-59608] - Lanes: 1. 45ug capan1 cell lysate 2. 45 ug HPAF cell lysate Primary, Antibody Dilution: 1 : 1000 Secondary Antibody: Anti-Rabbit HRP Secondary, Antibody Dilution: 1 : 5000 Gene name: CPT1A.")
![Western Blot: CPT1A Antibody [NBP1-59608] - 293T cells lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/CPT1A-Antibody-Western-Blot-NBP1-59608-img0002.jpg)
![Western Blot: CPT1A Antibody [NBP1-59608] - Sample Tissue: 293T, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1ug/ml, Peptide Concentration: 5ug/ml, Lysate Quantity: 25ug/lane/lane, Gel Concentration: 0.12](http://images.novusbio.com/fullsize/CPT1A-Antibody-Western-Blot-NBP1-59608-img0005.jpg)
![Immunohistochemistry-Paraffin: RBM17 Antibody [NBP2-97616] - Immunochemical staining of human RBM17 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/RBM17-Antibody-Immunohistochemistry-Paraffin-NBP2-97616-img0003.jpg "Immunohistochemistry-Paraffin: RBM17 Antibody [NBP2-97616] - Immunochemical staining of human RBM17 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Western Blot: RBM17 Antibody [NBP2-97616] - Anti-RBM17 rabbit polyclonal antibody at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate Lane B: Raji Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 45 kDa](http://images.novusbio.com/fullsize/RBM17-Antibody-Western-Blot-NBP2-97616-img0002.jpg)
![Immunoprecipitation: RBM17 Antibody [NBP2-97616] - RBM17 was immunoprecipitated using: Lane A: 0.5 mg Raji Whole Cell Lysate 4 ul anti-RBM17 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RBM17 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 45 kDa](http://images.novusbio.com/fullsize/RBM17-Antibody-Immunoprecipitation-NBP2-97616-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: L1CAM Antibody (014) [NBP2-89294] - Staining of Human CD171 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CD171 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.](http://images.novusbio.com/fullsize/L1CAM-Antibody-014-Immunocytochemistry-Immunofluorescence-NBP2-89294-img0003.jpg "Immunocytochemistry/Immunofluorescence: L1CAM Antibody (014) [NBP2-89294] - Staining of Human CD171 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CD171 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.")
![Immunohistochemistry-Paraffin: L1CAM Antibody (014) [NBP2-89294] - Staining of human CD171 in human appendix with rabbit monoclonal antibody (1:200). Positive staining was localized to myenteric nerve plexus.](http://images.novusbio.com/fullsize/L1CAM-Antibody-014-Immunohistochemistry-Paraffin-NBP2-89294-img0001.jpg)
![Flow Cytometry: L1CAM Antibody (014) [NBP2-89294] - Analysis of human CD171 expression on HeLa cells. Cells were stained with purified anti-Human CD171, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/L1CAM-Antibody-014-Flow-Cytometry-NBP2-89294-img0002.jpg)
![Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-98964] - Immunochemical staining of human SIN3A in human rectum with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/SIN3A-Antibody-Immunohistochemistry-Paraffin-NBP2-98964-img0004.jpg "Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-98964] - Immunochemical staining of human SIN3A in human rectum with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-98964] - Immunochemical staining of human SIN3A in human duodenum with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/SIN3A-Antibody-Immunohistochemistry-Paraffin-NBP2-98964-img0002.jpg)
![Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-98964] - Immunochemical staining of human SIN3A in human ovarian cancer with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/SIN3A-Antibody-Immunohistochemistry-Paraffin-NBP2-98964-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SIN3A Antibody [NBP2-98964] - Immunofluorescence staining of SIN3A in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98964 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus .](http://images.novusbio.com/fullsize/nbp2-98964_rabbit-polyclonal-sin3a-antibody-235202511545674.png)
![Western Blot: PPP2R5D Antibody (PSH01-22) [NBP3-32847] - Western blot analysis of PPP2R5D on different lysates with Rabbit anti-PPP2R5D antibody (NBP3-32847) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: NIH/3T3 cell lysate (20 ug/Lane) Lane 8: Human brain tissue lysate (40 ug/Lane) Lane 9: Mouse brain tissue lysate (40 ug/Lane) Lane 10: Rat brain tissue lysate (40 ug/Lane) Lane 11: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 70 kDa Observed band size: 56~70 kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32847) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32847_rabbit-ppp2r5d-mab-psh01-22-288202412461614.jpg "Western Blot: PPP2R5D Antibody (PSH01-22) [NBP3-32847] - Western blot analysis of PPP2R5D on different lysates with Rabbit anti-PPP2R5D antibody (NBP3-32847) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: NIH/3T3 cell lysate (20 ug/Lane) Lane 8: Human brain tissue lysate (40 ug/Lane) Lane 9: Mouse brain tissue lysate (40 ug/Lane) Lane 10: Rat brain tissue lysate (40 ug/Lane) Lane 11: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 70 kDa Observed band size: 56~70 kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32847) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PPP2R5D Antibody (PSH01-22) [NBP3-32847] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PPP2R5D antibody (NBP3-32847) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32847) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32847_rabbit-ppp2r5d-mab-psh01-22-298202414304124.jpg)
![Flow Cytometry: PPP2R5D Antibody (PSH01-22) [NBP3-32847] - Flow cytometric analysis of A431 cells labeling PPP2R5D. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32847, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32847_rabbit-ppp2r5d-mab-psh01-22-392024884664.jpg)
![Western Blot: Vimentin Antibody (001) [NBP2-89210] - Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:54 kDa Observed band size:55 kDa](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Western-Blot-NBP2-89210-img0001.jpg "Western Blot: Vimentin Antibody (001) [NBP2-89210] - Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:54 kDa Observed band size:55 kDa")
![Immunohistochemistry-Paraffin: Vimentin Antibody (001) [NBP2-89210] - Staining of human Vimentin in human lung cancer with rabbit monoclonal antibody at 1:10000 dilution.](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89210-img0004.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (001) [NBP2-89210] - Staining of human Vimentin in human stomach with rabbit monoclonal antibody at 1:10000.](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89210-img0002.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (001) [NBP2-89210] - Staining of human Vimentin in human malignant melanoma with rabbit monoclonal antibody at 1:10000 dilution.](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89210-img0003.jpg)
![Western Blot: CEND1 Antibody [NBP3-06496] - Lane A: SH-SY5Y Whole Cell LysateLane B: U-87 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:15 kDaObserved band size: kDa](http://images.novusbio.com/fullsize/CEND1-Antibody-Western-Blot-NBP3-06496-img0001.jpg "Western Blot: CEND1 Antibody [NBP3-06496] - Lane A: SH-SY5Y Whole Cell LysateLane B: U-87 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:15 kDaObserved band size: kDa")
![Immunohistochemistry-Paraffin: CEND1 Antibody [NBP3-06496] - Staining of human CEND1 in human brain with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/CEND1-Antibody-Immunohistochemistry-Paraffin-NBP3-06496-img0002.jpg)
![Immunoprecipitation: CEND1 Antibody [NBP3-06496] - Lane A:0.5 mg SH-SY5Y Whole Cell Lysate4 uL anti-CEND1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CEND1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 22 kDaObserved band size :22 kDa](http://images.novusbio.com/fullsize/CEND1-Antibody-Immunoprecipitation-NBP3-06496-img0003.jpg)
![Western Blot: GLRB Antibody [NBP2-97806] - Anti-GLRB rabbit polyclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 56 kDa. Observed band size: 72 kDa](http://images.novusbio.com/fullsize/GLRB-Antibody-Western-Blot-NBP2-97806-img0004.jpg "Western Blot: GLRB Antibody [NBP2-97806] - Anti-GLRB rabbit polyclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 56 kDa. Observed band size: 72 kDa")
![Immunohistochemistry-Paraffin: GLRB Antibody [NBP2-97806] - Immunochemical staining of human GLRB in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/GLRB-Antibody-Immunohistochemistry-Paraffin-NBP2-97806-img0003.jpg)
![Immunoprecipitation: GLRB Antibody [NBP2-97806] - GLRB was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-GLRB rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-GLRB rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 56 kDa. Observed band size: 72 kDa](http://images.novusbio.com/fullsize/GLRB-Antibody-Immunoprecipitation-NBP2-97806-img0005.jpg)
![Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1187] - Brain, Amygdala neurons](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Immunohistochemistry-Paraffin-NLS1187-img0008.jpg "Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1187] - Brain, Amygdala neurons")
![Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1187] - Analysis of anti-5HT2B Receptor antibody with human brain, neurons and glia.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Immunohistochemistry-Paraffin-NLS1187-img0003.jpg)
![Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1187] - Anti-HTR1B / 5-HT2B Receptor antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Immunohistochemistry-Paraffin-NLS1187-img0005.jpg)
![Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1187] - Human Lung, Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Immunohistochemistry-Paraffin-NLS1187-img0006.jpg)
![Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1187] - Brain, Substantia Nigra](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Immunohistochemistry-Paraffin-NLS1187-img0007.jpg)
![Flow Cytometry: HDAC6 Antibody (159) [NBP3-09113] - Separation of K562 cells stained using anti-HDAC6 (159) purified antibody (concentration in sample 4,0 ug/ml, GAM APC, red-filled) from K562 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).](http://images.novusbio.com/fullsize/HDAC6-Antibody-159-Flow-Cytometry-NBP3-09113-img0001.jpg "Flow Cytometry: HDAC6 Antibody (159) [NBP3-09113] - Separation of K562 cells stained using anti-HDAC6 (159) purified antibody (concentration in sample 4,0 ug/ml, GAM APC, red-filled) from K562 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).")
![Western Blot: Calcium-sensing R/CaSR Antibody (PSH03-47) [NBP3-32111] - Western blot analysis of Calcium-sensing R/CaSR on different lysates with Rabbit anti-Calcium-sensing R/CaSR antibody (NBP3-32111) at 1/2,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Mouse kidney tissue lysate (no heat) Lane 3: Rat kidney tissue lysate (no heat) Lysates/proteins at 40 ug/Lane. Predicted band size: 121 kDa Observed band size: 160 kDa Exposure time: 1 minute 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32111) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32111_rabbit-calcium-sensing-r-casr-mab-psh03-47-278202416331274.jpg "Western Blot: Calcium-sensing R/CaSR Antibody (PSH03-47) [NBP3-32111] - Western blot analysis of Calcium-sensing R/CaSR on different lysates with Rabbit anti-Calcium-sensing R/CaSR antibody (NBP3-32111) at 1/2,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Mouse kidney tissue lysate (no heat) Lane 3: Rat kidney tissue lysate (no heat) Lysates/proteins at 40 ug/Lane. Predicted band size: 121 kDa Observed band size: 160 kDa Exposure time: 1 minute 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32111) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Calcium-sensing R/CaSR Antibody (PSH03-47) [NBP3-32111] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Calcium-sensing R/CaSR antibody (NBP3-32111) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32111) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32111_rabbit-calcium-sensing-r-casr-mab-psh03-47-298202414333644.jpg)
![Immunohistochemistry: Calcium-sensing R/CaSR Antibody (PSH03-47) [NBP3-32111] - Immunofluorescence analysis of paraffin-embedded mouse kidney tissue labeling Calcium-sensing R/CaSR with Rabbit anti-Calcium-sensing R/CaSR antibody (NBP3-32111) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32111, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32111_rabbit-calcium-sensing-r-casr-mab-psh03-47-29820241492610.jpg)
![Western Blot: RCAN1 Antibody (JE62-71) [NBP3-32100] - Western blot analysis of RCAN1 on different lysates with Rabbit anti-RCAN1 antibody (NBP3-32100) at 1/1,000 dilution. Lane 1: SW480 cell lysate (20 ug/Lane) Lane 2: U-87 MG cell lysate (20 ug/Lane) Lane 3: SH-SY5Y cell lysate (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Lane 5: Rat brain tissue lysate (40 ug/Lane) Lane 6: Mouse brain tissue lysate (40 ug/Lane) Lane 7: Mouse thymus tissue lysate (40 ug/Lane) Lane 8: Rat cerebellum tissue lysate (40 ug/Lane) Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32100) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32100_rabbit-rcan1-mab-je62-71-278202415242658.jpg "Western Blot: RCAN1 Antibody (JE62-71) [NBP3-32100] - Western blot analysis of RCAN1 on different lysates with Rabbit anti-RCAN1 antibody (NBP3-32100) at 1/1,000 dilution. Lane 1: SW480 cell lysate (20 ug/Lane) Lane 2: U-87 MG cell lysate (20 ug/Lane) Lane 3: SH-SY5Y cell lysate (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Lane 5: Rat brain tissue lysate (40 ug/Lane) Lane 6: Mouse brain tissue lysate (40 ug/Lane) Lane 7: Mouse thymus tissue lysate (40 ug/Lane) Lane 8: Rat cerebellum tissue lysate (40 ug/Lane) Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32100) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: RCAN1 Antibody (JE62-71) [NBP3-32100] - Immunocytochemistry analysis of Jurkat cells labeling RCAN1 with Rabbit anti-RCAN1 antibody (NBP3-32100) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RCAN1 antibody (NBP3-32100) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32100_rabbit-rcan1-mab-je62-71-29202410234244.jpg)
![Flow Cytometry: RCAN1 Antibody (JE62-71) [NBP3-32100] - Flow cytometric analysis of Jurkat cells labeling RCAN1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32100, 1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32100_rabbit-rcan1-mab-je62-71-29202416372121.jpg)