| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-20224-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70216 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41271-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95089-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52520-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05122-0.025mg | |||
![Immunocytochemistry/Immunofluorescence: TRPM7 Antibody [NBP1-20224] - PC12 cells at a concentration of 30 ug/ml using Rabbit antibody to 4th cytoplasmic loop of human TRPM7 (LTRPC7): IgG , DAPI counterstained.](http://images.novusbio.com/fullsize/TRPM7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-20224-img0005.jpg "Immunocytochemistry/Immunofluorescence: TRPM7 Antibody [NBP1-20224] - PC12 cells at a concentration of 30 ug/ml using Rabbit antibody to 4th cytoplasmic loop of human TRPM7 (LTRPC7): IgG , DAPI counterstained.")
![Immunocytochemistry/Immunofluorescence: TRPM7 Antibody [NBP1-20224] - IF on PC12 cells at a concentration of 30 ug/ml using Rabbit antibody to 4th cytoplasmic loop of human TRPM7 (LTRPC7): IgG , DAPI counterstained appearing in blue.](http://images.novusbio.com/fullsize/TRPM7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-20224-img0004.jpg)
![Western Blot: SLC31A1/CTR1 Antibody [NBP3-12348] - 1:1000 antibody dilution in DiluObuffer. Labesl monomeric, dimeric and multimeric Human CTR1.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-Western-Blot-NBP3-12348-img0001.jpg "Western Blot: SLC31A1/CTR1 Antibody [NBP3-12348] - 1:1000 antibody dilution in DiluObuffer. Labesl monomeric, dimeric and multimeric Human CTR1.")
![Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody [NBP3-12348] - Mouse Lung. Mouse liver. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-Immunohistochemistry-Paraffin-NBP3-12348-img0005.jpg)
![Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody [NBP3-12348] - Mouse Liver. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 5X magnification.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-Immunohistochemistry-Paraffin-NBP3-12348-img0002.jpg)
![Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody [NBP3-12348] - Mouse liver. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-Immunohistochemistry-Paraffin-NBP3-12348-img0003.jpg)
![Western Blot: ASPA Antibody (3G5) [NBP2-46549] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASPA.](http://images.novusbio.com/fullsize/ASPA-Antibody-3G5-Western-Blot-NBP2-46549-img0005.jpg "Western Blot: ASPA Antibody (3G5) [NBP2-46549] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASPA.")
![Immunohistochemistry: ASPA Antibody (3G5) [NBP2-46549] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/ASPA-Antibody-3G5-Immunohistochemistry-NBP2-46549-img0004.jpg)
![Immunohistochemistry: ASPA Antibody (3G5) [NBP2-46549] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/ASPA-Antibody-3G5-Immunohistochemistry-NBP2-46549-img0001.jpg)
![Immunohistochemistry: ASPA Antibody (3G5) [NBP2-46549] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/ASPA-Antibody-3G5-Immunohistochemistry-NBP2-46549-img0002.jpg)
![Immunohistochemistry: ASPA Antibody (3G5) [NBP2-46549] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/ASPA-Antibody-3G5-Immunohistochemistry-NBP2-46549-img0003.jpg)
![Immunohistochemistry: Calcium-sensing R/CaSR Antibody - BSA Free [NBP2-41271] - Immunohistochemistry of Calcium-sensing R/CaSR in human kidney tissue with Calcium-sensing R/CaSR antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41271_rabbit-polyclonal-calcium-sensing-r-casr-antibody-271120241943225.jpg "Immunohistochemistry: Calcium-sensing R/CaSR Antibody - BSA Free [NBP2-41271] - Immunohistochemistry of Calcium-sensing R/CaSR in human kidney tissue with Calcium-sensing R/CaSR antibody at 5 ug/mL.")
![Immunocytochemistry/ Immunofluorescence: Calcium-sensing R/CaSR Antibody - BSA Free [NBP2-41271] - Immunofluorescence of Calcium-sensing R/CaSR in human kidney tissue with Calcium-sensing R/CaSR antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41271_rabbit-polyclonal-calcium-sensing-r-casr-antibody-271120241928876.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody - BSA Free [NBP2-41271] - Western blot analysis of Calcium-sensing R/CaSR in EL4 cell lysate with Calcium-sensing R/CaSR antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/nbp2-41271_rabbit-polyclonal-calcium-sensing-r-casr-antibody-271120242092044.gif)
![Immunocytochemistry/Immunofluorescence: Norrin/NDP Antibody [NBP1-59305] - Staining of vascular BM whole mounts with antibodies to proteins detected in the proteome analysis. A norrin-specific staining is shown to be generic for the entire vascular BM whole mounts (H), the signal, however, being less prominent in vascular aneurisms (arrow in H). Staining of vascular BM whole mounts from non-diabetic eyes showed a clearly weaker staining for norrin, when compared to vascular whole mounts from non-diabetic donors. Bar: 25um. Image collected and cropped by CiteAb from the following publication (journals.plos.org/plosone/article?id=10.1371/journal.pone.0189857), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Norrin-NDP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-59305-img0005.jpg "Immunocytochemistry/Immunofluorescence: Norrin/NDP Antibody [NBP1-59305] - Staining of vascular BM whole mounts with antibodies to proteins detected in the proteome analysis. A norrin-specific staining is shown to be generic for the entire vascular BM whole mounts (H), the signal, however, being less prominent in vascular aneurisms (arrow in H). Staining of vascular BM whole mounts from non-diabetic eyes showed a clearly weaker staining for norrin, when compared to vascular whole mounts from non-diabetic donors. Bar: 25um. Image collected and cropped by CiteAb from the following publication (journals.plos.org/plosone/article?id=10.1371/journal.pone.0189857), licensed under a CC-BY license.")
![Western Blot: NDP/Norrin Antibody [NBP1-59305] - Fetal Liver tissue lysate at a concentration of 1ug/ml.](http://images.novusbio.com/fullsize/NDP-Norrin-Antibody-Western-Blot-NBP1-59305-img0001.jpg)
![Immunohistochemistry-Paraffin: NDP/Norrin Antibody [NBP1-59305] - Human Brain, cortex tissue at an antibody concentration of 5ug/ml.](http://images.novusbio.com/fullsize/NDP-Norrin-Antibody-Immunohistochemistry-Paraffin-NBP1-59305-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Norrin/NDP Antibody [NBP1-59305] - Staining of vascular BM whole mounts with antibodies to proteins detected in the proteome analysis.A generic staining of the vascular BMs was given by an antibody to the 7S domain of collagen IV alpha 3 (A, C, E, F, G). Prominent staining for microvascular aneurisms was detected by staining with antibodies to C9 (B, C), Fibronectin (FN, E), ApoE (F) & PRELP (G). The same treatment of vascular BM whole mounts from non-diabetic eyes did not show staining for these proteins (D). A norrin-specific staining is shown to be generic for the entire vascular BM whole mounts (H), the signal, however, being less prominent in vascular aneurisms (arrow in H). Staining of vascular BM whole mounts from non-diabetic eyes showed a clearly weaker staining for norrin, when compared to vascular whole mounts from non-diabetic donors. Bar: 25um. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0189857), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-59305_rabbit-polyclonal-norrin-ndp-antibody-310202416182633.jpg)
![Western Blot: Galanin Antibody [NBP1-45217] - Staining of Mouse (A) and Rat (B) Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence](http://images.novusbio.com/fullsize/Galanin-Antibody-Western-Blot-NBP1-45217-img0003.jpg "Western Blot: Galanin Antibody [NBP1-45217] - Staining of Mouse (A) and Rat (B) Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence")
![Western Blot: Galanin Antibody [NBP1-45217] - Analysis of Galanin in Human Cerebellum lysate (35ug protein in RIPA buffer) using NBP1-45217 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Galanin-Antibody-Western-Blot-NBP1-45217-img0002.jpg)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95089] - Analysis of extracts of HeLa cells, using Sodium Potassium ATPase Alpha 1 at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL B](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-Western-Blot-NBP2-95089-img0004.jpg "Western Blot: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95089] - Analysis of extracts of HeLa cells, using Sodium Potassium ATPase Alpha 1 at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL B")
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95089] - Human stomach using ATP1A1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95089-img0002.jpg)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95089] - Analysis of extracts of various cell lines, using Sodium Potassium ATPase Alpha 1 at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-Western-Blot-NBP2-95089-img0005.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95089] - Human prostate using ATP1A1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95089-img0001.jpg)
![Immunoprecipitation: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95089] - Analysis of 200ug extracts of LO2 cells, using Sodium Potassium ATPase Alpha 1 at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-Immunoprecipitation-NBP2-95089-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: Calbindin D-28K Antibody (1K2K2) [NBP3-16209] - Confocal imaging of paraffin-embedded Mouse brain tissue using Calbindin D-28K Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16209_rabbit-calbindin-d-28k-mab-1k2k2-72202512435372.jpg)
![Western Blot: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis using NOS1 mAb against human NOS1 (AA: 17-153) recombinant protein. (Expected MW is 40.3 kDa)](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-Western-Blot-NBP2-52520-img0003.jpg "Western Blot: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis using NOS1 mAb against human NOS1 (AA: 17-153) recombinant protein. (Expected MW is 40.3 kDa)")
![Immunocytochemistry/Immunofluorescence: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis of HeLa cells using NOS1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-Immunofluorescence-NBP2-52520-img0002.jpg)
![Flow Cytometry: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis of HeLa cells using NOS1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-Flow-Cytometry-NBP2-52520-img0001.jpg)
![Western Blot: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis using NOS1 mAb against HEK293 (1) and NOS1 (AA: 17-153)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-Western-Blot-NBP2-52520-img0004.jpg)
![ELISA: nNOS Antibody (2E11G6) [NBP2-52520] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-ELISA-NBP2-52520-img0005.jpg)
![Immunohistochemistry: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Staining in human kidney. Strong staining of plasma of nephritis kidney was seen. Dilution: 1:75.](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-05122-img0011.jpg "Immunohistochemistry: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Staining in human kidney. Strong staining of plasma of nephritis kidney was seen. Dilution: 1:75.")
![Western Blot: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Lane 1: purified C3, lane 2: purifed C3 reduced/DTT, lane 3: purified C3a , lane 4: EDTA-plasma. Lane 5: zymosan-activated serum](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Western-Blot-NBP1-05122-img0004.jpg)
![Immunohistochemistry-Paraffin: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Staining of human kidney. Strong staining of veins of nephritis kidney was seen. Dilution: 1:150.](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Immunohistochemistry-Paraffin-NBP1-05122-img0007.jpg)
![Sandwich ELISA Capture: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - The calibration curve of a sandwich assay for Complement C3a using NBP1-05122 as the capture antibody and NBP2-23496 as the biotinylated detection antibody.](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Sandwich-ELISA-Capture-NBP1-05122-img0005.jpg)
![Immunohistochemistry: CNPase Antibody [NB100-1935] - A tissue section through an adult mouse brain showing CNPase (brown staining) in white matter tracts and the granule cell layer of the cerebellum.](http://images.novusbio.com/fullsize/CNPase-Antibody-Immunohistochemistry-NB100-1935-img0001.jpg "Immunohistochemistry: CNPase Antibody [NB100-1935] - A tissue section through an adult mouse brain showing CNPase (brown staining) in white matter tracts and the granule cell layer of the cerebellum.")
![CNPase Antibody [NB100-1935] - CNPase-positive oligodendrocytes (brown) in cerebellar white matter (adult mouse). Right: Western.](http://images.novusbio.com/fullsize/CNPase-Antibody-NB100-1935-img0002.jpg)
![Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Sodium Potassium ATPase Alpha 1 with Rabbit anti-Sodium Potassium ATPase Alpha 1 antibody (NBP3-33143) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33143, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33143_rabbit-sodium-potassium-atpase-alpha-1-mab-st0533-29820241420955.jpg "Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Sodium Potassium ATPase Alpha 1 with Rabbit anti-Sodium Potassium ATPase Alpha 1 antibody (NBP3-33143) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33143, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Sodium Potassium ATPase Alpha 1 antibody (NBP3-33143) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33143) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33143_rabbit-sodium-potassium-atpase-alpha-1-mab-st0533-298202413585572.jpg)
![Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Immunocytochemistry analysis of HeLa cells labeling Sodium Potassium ATPase Alpha 1 with Rabbit anti-Sodium Potassium ATPase Alpha 1 Alpha 1 antibody (NBP3-33143) at 1/500 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Sodium Potassium ATPase antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33143_rabbit-sodium-potassium-atpase-alpha-1-mab-st0533-29202410234287.jpg)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Western blot analysis of Sodium Potassium ATPase Alpha 1 on different lysates with Rabbit anti-Sodium Potassium ATPase Alpha 1 antibody (NBP3-33143) at 1/100,000 dilution. Lane 1: HeLa cell lysate (no heat) (20 µg/Lane) Lane 2: HT-29 cell lysate (no heat) (20 µg/Lane) Lane 3: HepG2 cell lysate (no heat) (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (no heat) (20 µg/Lane) Lane 5: L-929 cell lysate (no heat) (20 µg/Lane) Lane 6: Mouse brain tissue lysate (no heat) (20 µg/Lane) Lane 7: Rat brain tissue lysate (no heat) (20 µg/Lane) Predicted band size: 113 kDa Observed band size: 100 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/100,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33143_rabbit-sodium-potassium-atpase-alpha-1-mab-st0533-392024848504.jpg)