| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11989-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62554-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26490-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26441-50ul | |||
![Immunocytochemistry/Immunofluorescence: TIM-3 Antibody (2C12) - Chimeric [NBP3-11989] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 2C12 (NBP3-11989) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11989, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/TIM-3-Antibody-2C12-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-11989-img0001.jpg "Immunocytochemistry/Immunofluorescence: TIM-3 Antibody (2C12) - Chimeric [NBP3-11989] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 2C12 (NBP3-11989) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11989, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![ELISA: TIM-3 Antibody (2C12) - Chimeric - Azide and BSA Free [NBP3-11989] - Binding curves of NBP3-11989 (blue line) and isotype control (NBP2-52638; red line) to an ELISA plate coated with mouse TIM-3-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 3000 to 0.0169 ng/ml was performed using NBP3-11989. For signal detection, anti-rabbit IgG1 HRP antibody was used.](http://images.novusbio.com/fullsize/nbp3-11989_rabbit-monoclonal-tim-3-antibody-2c12-chimeric-74202522113036.png)
![Flow Cytometry: TIM-3 Antibody (2C12) - Chimeric - Azide and BSA Free [NBP3-11989] - Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-11989 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-11989_rabbit-monoclonal-tim-3-antibody-2c12-chimeric-74202522113017.png)
![Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Western-Blot-NBP2-62554-img0003.jpg "Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.")
![Immunohistochemistry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Immunohistochemical staining of human placenta tissue using anti-CD34 antibody. My10 Anti-CD34 staining of paraffin embedded human placenta tissue using the rabbit-chimeric version of My10 (NBP2-62554). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62554 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Strong endothelial staining of within villi may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Immunohistochemistry-NBP2-62554-img0002.jpg)
![Flow Cytometry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Flow-cytometry using anti-CD34 antibody My10. Human leukocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of My10 (NBP2-62554, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Flow-Cytometry-NBP2-62554-img0001.jpg)
![In vitro assay: Recombinant Human SOD1/Cu-Zn SOD Pre-formed Fibrils Protein [NBP3-14783] - Thioflavin T assay of SOD1 pre-formed fibrils (PFFs) (NBP3-14783) combined with SOD1 monomers . Pre-formed fibrils seed the aggregation of monomers, leading to an increase in Thioflavin T fluorescence](http://images.novusbio.com/fullsize/Recombinant-Human-SOD1-Cu-Zn-SOD-Pre-formed-Fibrils-Protein-In-vitro-assay-NBP3-14783-img0001.jpg "In vitro assay: Recombinant Human SOD1/Cu-Zn SOD Pre-formed Fibrils Protein [NBP3-14783] - Thioflavin T assay of SOD1 pre-formed fibrils (PFFs) (NBP3-14783) combined with SOD1 monomers . Pre-formed fibrils seed the aggregation of monomers, leading to an increase in Thioflavin T fluorescence")
![Electron Microscopy: Recombinant Human SOD1/Cu-Zn SOD Pre-formed Fibrils Protein [NBP3-14783] - TEM of human SOD1 pre-formed fibrils (PFFs)](http://images.novusbio.com/fullsize/Recombinant Human SOD1-Cu-Zn SOD Pre-formed Fibrils Protein-Electron Microscopy-NBP3-14783-img0003.jpg)
![Electron Microscopy: Recombinant Human SOD1/Cu-Zn SOD Pre-formed Fibrils Protein [NBP3-14783] - TEM of human SOD1 pre-formed fibrils (PFFs)](http://images.novusbio.com/fullsize/Recombinant-Human-SOD1-Cu-Zn-SOD-Pre-formed-Fibrils-Protein-Electron-Microscopy-NBP3-14783-img0002.jpg)
![Western Blot: Neuropilin-1 Antibody [NBP3-23182] - Western blot image of COS-7 cells untransfected (lanes 1 & 3) or transfected with HA-tagged mouse neuropilin-1 (lanes 2 & 4). Blots were probed with anti-Neuropilin-1 (NBP3-23182) (lanes 1 & 2) or with anti-HA (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23182_rabbit-neuropilin-1-pab-199202312523619.jpg "Western Blot: Neuropilin-1 Antibody [NBP3-23182] - Western blot image of COS-7 cells untransfected (lanes 1 & 3) or transfected with HA-tagged mouse neuropilin-1 (lanes 2 & 4). Blots were probed with anti-Neuropilin-1 (NBP3-23182) (lanes 1 & 2) or with anti-HA (lanes 3 & 4).")
![Immunocytochemistry/Immunofluorescence: Neuropilin-1 Antibody [NBP3-23182] - Immunocytochemical double labeling using anti-HA mouse monoclonal and anti-NRP-1 rabbit polyclonal (NBP3-23182) antibodies in COS-7 cells mock transfected (A,D) or transfected with NRP-1 constructs (B,E). The specificity of the binding in E was demonstrated by using NRP-1 peptide in the presence of the anti-NRP-1 antibody (C,F).](http://images.novusbio.com/fullsize/nbp3-23182_rabbit-neuropilin-1-pab-19920231856956.jpg)
![Immunohistochemistry-Paraffin: ACTH Antibody (CLIP/1407) - Azide and BSA Free [NBP2-47683] - Formalin-fixed, paraffin-embedded Human Pituitary stained with ACTH Monoclonal Antibody (CLIP/1407).](http://images.novusbio.com/fullsize/ACTH-Antibody-CLIP-1407-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47683-img0002.jpg "Immunohistochemistry-Paraffin: ACTH Antibody (CLIP/1407) - Azide and BSA Free [NBP2-47683] - Formalin-fixed, paraffin-embedded Human Pituitary stained with ACTH Monoclonal Antibody (CLIP/1407).")
![Immunohistochemistry-Paraffin: ACTH Antibody (CLIP/1407) - Azide and BSA Free [NBP2-47683] - Formalin-fixed, paraffin-embedded human pituitary adenoma stained with ACTH Mouse Monoclonal Antibody (CLIP/1407).](http://images.novusbio.com/fullsize/ACTH-Antibody-CLIP-1407-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47683-img0003.jpg)
![Immunohistochemistry-Paraffin: ACTH Antibody (CLIP/1407) - Azide and BSA Free [NBP2-47683] - Formalin-fixed, paraffin-embedded human pituitary gland stained with ACTH Mouse Monoclonal Antibody (CLIP/1407).](http://images.novusbio.com/fullsize/ACTH-Antibody-CLIP-1407-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47683-img0004.jpg)
![Western Blot: APPL Antibody [NBP2-15434] - Sample (30 ug of whole cell lysate) A: MCF-7 7.5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/APPL-Antibody-Western-Blot-NBP2-15434-img0002.jpg "Western Blot: APPL Antibody [NBP2-15434] - Sample (30 ug of whole cell lysate) A: MCF-7 7.5% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: APPL Antibody [NBP2-15434] - Mouse muscle. APPL1 antibody [N1N2], N-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/APPL-Antibody-Immunohistochemistry-Paraffin-NBP2-15434-img0003.jpg)
![Immunohistochemistry-Paraffin: APPL Antibody [NBP2-15434] - AZ521 xenograft, using APPL1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/APPL-Antibody-Immunohistochemistry-Paraffin-NBP2-15434-img0001.jpg)
![Immunohistochemistry: ATPB Antibody (5F10) [NBP3-26490] - Image of ATPB Antibody (5F10) diluted at 1:100 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26490_rabbit-atpb-mab-5f10-262202415355720.jpg "Immunohistochemistry: ATPB Antibody (5F10) [NBP3-26490] - Image of ATPB Antibody (5F10) diluted at 1:100 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: ATPB Antibody (5F10) [NBP3-26490] - Image of ATPB Antibody (5F10) diluted at 1:100 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26490_rabbit-atpb-mab-5f10-27220248471912.jpg)
![Western Blot: ATPB Antibody (5F10) [NBP3-26490] - Positive Western Blot detected in: 293T whole cell lysate, HT29 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, Rat Heart tissue, Mouse Heart tissue. All lanes: ATPB Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 57 kDa Observed band size: 57 kDa](http://images.novusbio.com/fullsize/nbp3-26490_rabbit-atpb-mab-5f10-282202411363015.jpg)
![Immunohistochemistry: Pannexin-1 Antibody (28G5) [NBP3-26441] - Image of Pannexin-1 Antibody (28G5) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.1:50% DAB.](http://images.novusbio.com/fullsize/nbp3-26441_rabbit-pannexin-1-mab-28g5-262202414225215.jpg "Immunohistochemistry: Pannexin-1 Antibody (28G5) [NBP3-26441] - Image of Pannexin-1 Antibody (28G5) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.1:50% DAB.")
![Immunohistochemistry: Pannexin-1 Antibody (28G5) [NBP3-26441] - Image of Pannexin-1 Antibody (28G5) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.1:50% DAB.](http://images.novusbio.com/fullsize/nbp3-26441_rabbit-pannexin-1-mab-28g5-2722024847192.jpg)
![Immunocytochemistry/Immunofluorescence: Pannexin-1 Antibody (28G5) [NBP3-26441] - Staining of U251 with Pannexin-1 Antibody (28G5) at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 502-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26441_rabbit-pannexin-1-mab-28g5-282202412493632.jpg)
![Flow Cytometry: Pannexin-1 Antibody (28G5) [NBP3-26441] - Overlay Peak curve showing A549 cells stained with Pannexin-1 Antibody (28G5) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26441_rabbit-pannexin-1-mab-28g5-282202414312766.jpg)
![Western Blot: VAMP-1 Antibody [NBP2-15159] - Sample (30 ug of whole cell lysate) A: NT2D1 15% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/VAMP-1-Antibody-Western-Blot-NBP2-15159-img0005.jpg "Western Blot: VAMP-1 Antibody [NBP2-15159] - Sample (30 ug of whole cell lysate) A: NT2D1 15% SDS PAGE, antibody diluted at 1:500.")
![Immunohistochemistry-Paraffin: VAMP-1 Antibody [NBP2-15159] - VAMP-1 antibody detects VAMP-1 protein on mouse fore at Dilution: 1:500.](http://images.novusbio.com/fullsize/VAMP-1-Antibody-Immunohistochemistry-Paraffin-NBP2-15159-img0008.jpg)
![Immunohistochemistry-Paraffin: VAMP-1 Antibody [NBP2-15159] - Paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/VAMP-1-Antibody-Immunohistochemistry-Paraffin-NBP2-15159-img0004.jpg)
![Western Blot: CYP46A1 Antibody [NBP2-16066] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP46A1 antibody [N1C1] (NBP2-16066) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16066_rabbit-polyclonal-cyp46a1-antibody-91020242014662.jpg "Western Blot: CYP46A1 Antibody [NBP2-16066] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP46A1 antibody [N1C1] (NBP2-16066) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: CYP46A1 Antibody [NBP2-16066] - CYP46A1 antibody [N1C1] detects CYP46A1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse hippocampus.CYP46A1 stained by CYP46A1 antibody [N1C1] (NBP2-16066) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16066_rabbit-polyclonal-cyp46a1-antibody-910202420104468.jpg)
![Immunohistochemistry-Paraffin: CYP46A1 Antibody [NBP2-16066] - CYP46A1 antibody [N1C1] detects CYP46A1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse hippocampus.CYP46A1 stained by CYP46A1 antibody [N1C1] (NBP2-16066) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16066_rabbit-polyclonal-cyp46a1-antibody-910202419593547.jpg)
![Western Blot: WWOX Antibody [NBP2-32122] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-WWOX antibody NBP2-32122 used for IP at 6 ug/mg lysate. WWOX was also immunoprecipitated by rabbit anti-WWOX antibody. For blotting immunoprecipitated WWOX, NBP2-32122 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/WWOX-Antibody-Western-Blot-NBP2-32122-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: WWOX Antibody [NBP2-32122] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-WWOX antibody NBP2-32122 used for IP at 6 ug/mg lysate. WWOX was also immunoprecipitated by rabbit anti-WWOX antibody. For blotting immunoprecipitated WWOX, NBP2-32122 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: WWOX Antibody [NBP2-32122] - Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- WWOX used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-NBP2-32122-img0003.jpg)
![Immunohistochemistry: WWOX Antibody [NBP2-32122] - Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- WWOX used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-NBP2-32122-img0002.jpg)