| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90116-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89836-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00344-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97692-50ul | |||
![Western Blot: CHIP/STUB1 Antibody (034) [NBP2-90116] - Anti-CHIP/STUB1 rabbit monoclonal antibody at 1:500 dilution. Lane A: HELA Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lane C: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-034-Western-Blot-NBP2-90116-img0005.jpg "Western Blot: CHIP/STUB1 Antibody (034) [NBP2-90116] - Anti-CHIP/STUB1 rabbit monoclonal antibody at 1:500 dilution. Lane A: HELA Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lane C: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: CHIP/STUB1 Antibody (034) [NBP2-90116] - Flow cytometric analysis of Human STUB1 expression on Hela cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human STUB1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-034-Flow-Cytometry-NBP2-90116-img0004.jpg)
![Immunoprecipitation: CHIP/STUB1 Antibody (034) [NBP2-90116] - STUB1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg 293T Whole Cell LysateLane C:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-STUB1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-STUB1 rabbit monoclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size: 35 kDa](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-034-Immunoprecipitation-NBP2-90116-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CHIP/STUB1 Antibody (034) [NBP2-90116] - Immunofluorescence staining of STUB1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-90116 antibody (dilution ratio 1:300) overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Nucleus.](http://images.novusbio.com/fullsize/nbp2-90116_rabbit-monoclonal-chip-stub1-antibody-034-235202514331566.png)
![Western Blot: Pannexin-1 Antibody (JE38-97) [NBP3-32714] - Western blot analysis of Pannexin-1 on different lysates with Rabbit anti-Pannexin-1 antibody (NBP3-32714) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: U-87 MG cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: SH-SY5Y cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: C2C12 cell lysate (20 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Lane 9: Mouse brain tissue lysate (40 ug/Lane) Predicted band size: 48 kDa Observed band size: 50 kDa Exposure time: 1 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32714) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32714_rabbit-pannexin-1-mab-je38-97-278202415212479.jpg "Western Blot: Pannexin-1 Antibody (JE38-97) [NBP3-32714] - Western blot analysis of Pannexin-1 on different lysates with Rabbit anti-Pannexin-1 antibody (NBP3-32714) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: U-87 MG cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: SH-SY5Y cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: C2C12 cell lysate (20 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Lane 9: Mouse brain tissue lysate (40 ug/Lane) Predicted band size: 48 kDa Observed band size: 50 kDa Exposure time: 1 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32714) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Pannexin-1 Antibody (JE38-97) [NBP3-32714] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Pannexin-1 antibody (NBP3-32714) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32714) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32714_rabbit-pannexin-1-mab-je38-97-298202413585529.jpg)
![Immunocytochemistry/ Immunofluorescence: Pannexin-1 Antibody (JE38-97) [NBP3-32714] - Immunocytochemistry analysis of 293T cells labeling Pannexin-1 with Rabbit anti-Pannexin-1 antibody (NBP3-32714) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Pannexin-1 antibody (NBP3-32714) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32714_rabbit-pannexin-1-mab-je38-97-292024111486.jpg)
![Western Blot: DLG1 Antibody (JE39-74) [NBP3-32929] - Western blot analysis of DLG1 on different lysates with Rabbit anti-DLG1 antibody (NBP3-32929) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HepG2 cell lysate Lane 3: U-87 MG cell lysate Lane 4: SiHa cell lysate Lane 5: NCI-H226 cell lysate Lane 6: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 100 kDa Observed band size: 120 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32929) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32929_rabbit-dlg1-mab-je39-74-288202412442920.jpg "Western Blot: DLG1 Antibody (JE39-74) [NBP3-32929] - Western blot analysis of DLG1 on different lysates with Rabbit anti-DLG1 antibody (NBP3-32929) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HepG2 cell lysate Lane 3: U-87 MG cell lysate Lane 4: SiHa cell lysate Lane 5: NCI-H226 cell lysate Lane 6: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 100 kDa Observed band size: 120 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32929) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: DLG1 Antibody (JE39-74) [NBP3-32929] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-DLG1 antibody (NBP3-32929) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32929) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32929_rabbit-dlg1-mab-je39-74-298202414161898.jpg)
![Flow Cytometry: DLG1 Antibody (JE39-74) [NBP3-32929] - Flow cytometric analysis of SiHa cells labeling DLG1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32929, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32929_rabbit-dlg1-mab-je39-74-392024884663.jpg)
![Western Blot: KCNK9 Antibody [NBP2-84109] - Host: Rabbit. Target Name: KCNK9. Sample Tissue: Human 293T. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/KCNK9-Antibody-Western-Blot-NBP2-84109-img0003.jpg "Western Blot: KCNK9 Antibody [NBP2-84109] - Host: Rabbit. Target Name: KCNK9. Sample Tissue: Human 293T. Antibody Dilution: 1.0ug/ml")
![Immunohistochemistry: KCNK9 Antibody [NBP2-84109]](http://images.novusbio.com/fullsize/KCNK9-Antibody-Immunohistochemistry-NBP2-84109-img0005.jpg)
![Western Blot: KCNK9 Antibody [NBP2-84109] - Host: Rabbit. Target Name: KCNK9. Sample Tissue: Human Hela Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/KCNK9-Antibody-Western-Blot-NBP2-84109-img0004.jpg)
![Immunohistochemistry: KCNK9 Antibody [NBP2-84109] - Immunohistochemistry with prostate cell lysate tissue at an antibody concentration of 5.0ug/ml using anti-KCNK9 antibody](http://images.novusbio.com/fullsize/KCNK9-Antibody-Immunohistochemistry-NBP2-84109-img0001.jpg)
![Immunohistochemistry: KCNK9 Antibody [NBP2-84109] - Immunohistochemistry with Human Prostate lysate tissue at an antibody concentration of 5.0ug/ml using anti-KCNK9 antibody](http://images.novusbio.com/fullsize/KCNK9-Antibody-Immunohistochemistry-NBP2-84109-img0002.jpg)
![Western Blot: Zic2 Antibody [NBP2-86425] - Host: Rabbit. Target Name: ZIC2. Sample Tissue: Human HT1080 Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/Zic2-Antibody-Western-Blot-NBP2-86425-img0004.jpg "Western Blot: Zic2 Antibody [NBP2-86425] - Host: Rabbit. Target Name: ZIC2. Sample Tissue: Human HT1080 Whole Cell. Antibody Dilution: 1ug/ml")
![Immunohistochemistry: Zic2 Antibody [NBP2-86425] - Human Skin](http://images.novusbio.com/fullsize/Zic2-Antibody-Immunohistochemistry-NBP2-86425-img0001.jpg)
![Western Blot: Zic2 Antibody [NBP2-86425] - WB Suggested Anti-ZIC2 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate](http://images.novusbio.com/fullsize/Zic2-Antibody-Western-Blot-NBP2-86425-img0003.jpg)
![Immunoprecipitation: Zic2 Antibody [NBP2-86425] - ZIC2 was immunoprecipitated from 1 mg HEK293 Whole Cell Lysate with NBP2-86425 with 1:200 dilution. Western blot was performed using NBP2-86425 at 1/1000 dilution. Lane 1: Control IP in HEK293 Whole Cell Lysate. Lane 2: ZIC2 IP with NBP2-86425](http://images.novusbio.com/fullsize/Zic2-Antibody-Immunoprecipitation-NBP2-86425-img0005.jpg)
![Western Blot: CRABP2 Antibody (004) [NBP2-89836] - Lane A: 293T Whole Cell Lysate Lane B: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:16 kDa Observed band size:14 kDa](http://images.novusbio.com/fullsize/CRABP2-Antibody-004-Western-Blot-NBP2-89836-img0002.jpg "Western Blot: CRABP2 Antibody (004) [NBP2-89836] - Lane A: 293T Whole Cell Lysate Lane B: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:16 kDa Observed band size:14 kDa")
![<b>Biological Strategies Validation. </b>Flow Cytometry: CRABP2 Antibody (004) [NBP2-89836] - Analysis of Human CRABP2 expression on MCF-7 cells. The cells were treated , stained with purified anti-Human CRABP2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/CRABP2-Antibody-004-Flow-Cytometry-NBP2-89836-img0004.jpg)
![Immunoprecipitation: CRABP2 Antibody (004) [NBP2-89836] - Lane A:0.5 mg 293T Whole Cell Lysate Lane B:0.5 mg Raji Whole Cell Lysate0.5 uL anti-CRABP2 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-CRABP2 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 16 kDa Observed band size: 16 kDa](http://images.novusbio.com/fullsize/CRABP2-Antibody-004-Immunoprecipitation-NBP2-89836-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: CRABP2 Antibody (004) [NBP2-89836] - Staining of Human CRABP2 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89836 antibody (1:300) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89836_rabbit-monoclonal-crabp2-antibody-004-235202514331567.png)
![Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunofluorescence analysis of paraffin-embedded human lymph nodes tissue labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32498, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-298202413585592.jpg "Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunofluorescence analysis of paraffin-embedded human lymph nodes tissue labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32498, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32498) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-29820241435258.jpg)
![Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunocytochemistry analysis of HeLa cells labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67/MKI67 antibody (NBP3-32498) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-2920241192339.jpg)
![Western Blot: KSR1 Antibody (JE57-75) [NBP3-32512] - Western blot analysis of KSR1 on different lysates with Rabbit anti-KSR1 antibody (NBP3-32512) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: HT-29 cell lysate Lane 4: HDLM-2 cell lysate Lane 5: C2C12 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 102 kDa Observed band size: 120/100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32512) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32512_rabbit-ksr1-mab-je57-75-27820241631620.jpg "Western Blot: KSR1 Antibody (JE57-75) [NBP3-32512] - Western blot analysis of KSR1 on different lysates with Rabbit anti-KSR1 antibody (NBP3-32512) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: HT-29 cell lysate Lane 4: HDLM-2 cell lysate Lane 5: C2C12 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 102 kDa Observed band size: 120/100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32512) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: KSR1 Antibody (JE57-75) [NBP3-32512] - Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with Rabbit anti-KSR1 antibody (NBP3-32512) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32512) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32512_rabbit-ksr1-mab-je57-75-29820241435283.jpg)
![Flow Cytometry: KSR1 Antibody (JE57-75) [NBP3-32512] - Flow cytometric analysis of C6 cells labeling KSR1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32512, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32512_rabbit-ksr1-mab-je57-75-29202416372119.jpg)
![Western Blot: KIF3A Antibody (JE31-39) [NBP3-32501] - Western blot analysis of KIF3A on different lysates with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Rat brain tissue lysate (40 ug/Lane) Lane 3: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 80 kDa Observed band size: 80 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32501) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32501_rabbit-kif3a-mab-je31-39-278202415295563.jpg "Western Blot: KIF3A Antibody (JE31-39) [NBP3-32501] - Western blot analysis of KIF3A on different lysates with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Rat brain tissue lysate (40 ug/Lane) Lane 3: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 80 kDa Observed band size: 80 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32501) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: KIF3A Antibody (JE31-39) [NBP3-32501] - Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32501) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32501_rabbit-kif3a-mab-je31-39-298202413585569.jpg)
![Immunocytochemistry/ Immunofluorescence: KIF3A Antibody (JE31-39) [NBP3-32501] - Immunocytochemistry analysis of NIH/3T3 cells labeling KIF3A with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32501_rabbit-kif3a-mab-je31-39-29202410334094.jpg)
![Western Blot: ZEB1 Antibody (PSH0-61) [NBP3-33133] - Western blot analysis of ZEB1 on different lysates with Rabbit anti-ZEB1 antibody (NBP3-33133) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: SK-OV-3 cell lysate Lane 3: HeLa cell lysate Lane 4: HEK-293 cell lysate Lane 5: U87-MG cell lysate Lane 6: MG-63 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 124 kDa Observed band size: 200 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33133) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33133_rabbit-zeb1-mab-psh0-61-28820241423310.jpg "Western Blot: ZEB1 Antibody (PSH0-61) [NBP3-33133] - Western blot analysis of ZEB1 on different lysates with Rabbit anti-ZEB1 antibody (NBP3-33133) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: SK-OV-3 cell lysate Lane 3: HeLa cell lysate Lane 4: HEK-293 cell lysate Lane 5: U87-MG cell lysate Lane 6: MG-63 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 124 kDa Observed band size: 200 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33133) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ZEB1 Antibody (PSH0-61) [NBP3-33133] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-ZEB1 antibody (NBP3-33133) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33133) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33133_rabbit-zeb1-mab-psh0-61-298202414262614.jpg)
![Flow Cytometry: ZEB1 Antibody (PSH0-61) [NBP3-33133] - Flow cytometric analysis of Jurkat cells labeling ZEB1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33133, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33133_rabbit-zeb1-mab-psh0-61-2920241645327.jpg)
![Immunohistochemistry-Paraffin: ATP5D Antibody [NBP3-00344] - Immunochemical staining of human ATP5D in human lung cancer with NBP3-00344 (1:5000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00344_rabbit-polyclonal-atp5d-antibody-23520251129340.png "Immunohistochemistry-Paraffin: ATP5D Antibody [NBP3-00344] - Immunochemical staining of human ATP5D in human lung cancer with NBP3-00344 (1:5000, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: ATP5D Antibody [NBP3-00344] - Immunochemical staining of human ATP5D in human hepatoma with NBP3-00344 (1:5000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00344_rabbit-polyclonal-atp5d-antibody-235202516144339.png)
![Western Blot: ATP5D Antibody [NBP3-00344] - NBP3-00344 at 1:500 dilution Lane A: HEPG2 Whole Cell Lysate Lane B: Raji Whole Cell Lysate Lane C: MOLT-4 Whole Cell Lysate Lane D: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:18 kDa Observed band size:14 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/nbp3-00344_rabbit-polyclonal-atp5d-antibody-235202514492339.png)
![Immunoprecipitation: ATP5D Antibody [NBP3-00344] - ATP5D was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate Lane B:0.5 mg Raji Whole Cell Lysate Lane C:0.5 mg A549 Whole Cell Lysate 2 uL NBP3-00344 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00344, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 13 kDa Observed band size: 13 kDa](http://images.novusbio.com/fullsize/nbp3-00344_rabbit-polyclonal-atp5d-antibody-235202516335022.png)
![Western Blot: PCYT1A Antibody [NBP2-97692] - Anti-PCYT1A rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lane C: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 45 kDa](http://images.novusbio.com/fullsize/PCYT1A-Antibody-Western-Blot-NBP2-97692-img0005.jpg "Western Blot: PCYT1A Antibody [NBP2-97692] - Anti-PCYT1A rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lane C: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 45 kDa")
![Immunohistochemistry-Paraffin: PCYT1A Antibody [NBP2-97692] - Staining of human PCYT1A in human skin with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PCYT1A-Antibody-Immunohistochemistry-Paraffin-NBP2-97692-img0004.jpg)
![Immunohistochemistry-Paraffin: PCYT1A Antibody [NBP2-97692] - Staining of human PCYT1A in human esophagus with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PCYT1A-Antibody-Immunohistochemistry-Paraffin-NBP2-97692-img0003.jpg)
![Immunoprecipitation: PCYT1A Antibody [NBP2-97692] - PCYT1A was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 4 ul anti-PCYT1A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PCYT1A rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 50 kDa](http://images.novusbio.com/fullsize/PCYT1A-Antibody-Immunoprecipitation-NBP2-97692-img0006.jpg)