| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06496-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89210-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-05805-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12686-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12563-50ul | |||
![Western Blot: CEND1 Antibody [NBP3-06496] - Lane A: SH-SY5Y Whole Cell LysateLane B: U-87 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:15 kDaObserved band size: kDa](http://images.novusbio.com/fullsize/CEND1-Antibody-Western-Blot-NBP3-06496-img0001.jpg "Western Blot: CEND1 Antibody [NBP3-06496] - Lane A: SH-SY5Y Whole Cell LysateLane B: U-87 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:15 kDaObserved band size: kDa")
![Immunohistochemistry-Paraffin: CEND1 Antibody [NBP3-06496] - Staining of human CEND1 in human brain with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/CEND1-Antibody-Immunohistochemistry-Paraffin-NBP3-06496-img0002.jpg)
![Immunoprecipitation: CEND1 Antibody [NBP3-06496] - Lane A:0.5 mg SH-SY5Y Whole Cell Lysate4 uL anti-CEND1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CEND1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 22 kDaObserved band size :22 kDa](http://images.novusbio.com/fullsize/CEND1-Antibody-Immunoprecipitation-NBP3-06496-img0003.jpg)
![Western Blot: Vimentin Antibody (001) [NBP2-89210] - Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:54 kDa Observed band size:55 kDa](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Western-Blot-NBP2-89210-img0001.jpg "Western Blot: Vimentin Antibody (001) [NBP2-89210] - Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:54 kDa Observed band size:55 kDa")
![Immunohistochemistry-Paraffin: Vimentin Antibody (001) [NBP2-89210] - Staining of human Vimentin in human lung cancer with rabbit monoclonal antibody at 1:10000 dilution.](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89210-img0004.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (001) [NBP2-89210] - Staining of human Vimentin in human stomach with rabbit monoclonal antibody at 1:10000.](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89210-img0002.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (001) [NBP2-89210] - Staining of human Vimentin in human malignant melanoma with rabbit monoclonal antibody at 1:10000 dilution.](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89210-img0003.jpg)
![Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody (11D5-R) [NBP3-32411] - Western blot analysis of Glutathione S-Transferase pi 1/GSTP1 on different lysates with Mouse anti-Glutathione S-Transferase pi 1/GSTP1 antibody (NBP3-32411) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: HT-29 cell lysate Lane 5: Jurkat cell lysate Lane 6: K-562 cell lysate Lane 7: JAR cell lysate Lane 8: Human brain tissue lysate Lane 9: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32411) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32411_mouse-glutathione-s-transferase-pi-1-gstp1-mab-11d5-r-278202416331264.jpg "Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody (11D5-R) [NBP3-32411] - Western blot analysis of Glutathione S-Transferase pi 1/GSTP1 on different lysates with Mouse anti-Glutathione S-Transferase pi 1/GSTP1 antibody (NBP3-32411) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: HT-29 cell lysate Lane 5: Jurkat cell lysate Lane 6: K-562 cell lysate Lane 7: JAR cell lysate Lane 8: Human brain tissue lysate Lane 9: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32411) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Glutathione S-Transferase pi 1/GSTP1 Antibody (11D5-R) [NBP3-32411] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-Glutathione S-Transferase pi 1/GSTP1 antibody (NBP3-32411) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32411) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32411_mouse-glutathione-s-transferase-pi-1-gstp1-mab-11d5-r-29820241435285.jpg)
![Immunocytochemistry/ Immunofluorescence: Glutathione S-Transferase pi 1/GSTP1 Antibody (11D5-R) [NBP3-32411] - Immunocytochemistry analysis of HT-29 cells labeling Glutathione S-Transferase pi 1/GSTP1 with Mouse anti-Glutathione S-Transferase pi 1/GSTP1 antibody (NBP3-32411) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Glutathione S-Transferase pi 1/GSTP1 antibody (NBP3-32411) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32411_mouse-glutathione-s-transferase-pi-1-gstp1-mab-11d5-r-2920241192326.jpg)
![Western Blot: Neurogranin Antibody (PSH02-96) [NBP3-32645] - Western blot analysis of Neurogranin on different lysates with Rabbit anti-Neurogranin antibody (NBP3-32645) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse hippocampus tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Rat hippocampus tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 8 kDa Observed band size: 15 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32645) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32645_rabbit-neurogranin-mab-psh02-96-278202415242663.jpg "Western Blot: Neurogranin Antibody (PSH02-96) [NBP3-32645] - Western blot analysis of Neurogranin on different lysates with Rabbit anti-Neurogranin antibody (NBP3-32645) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse hippocampus tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Rat hippocampus tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 8 kDa Observed band size: 15 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32645) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Neurogranin Antibody (PSH02-96) [NBP3-32645] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Neurogranin antibody (NBP3-32645) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32645) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32645_rabbit-neurogranin-mab-psh02-96-29820241420998.jpg)
![Immunohistochemistry: Neurogranin Antibody (PSH02-96) [NBP3-32645] - Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling Neurogranin with Rabbit anti-Neurogranin antibody (NBP3-32645) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32645, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32645_rabbit-neurogranin-mab-psh02-96-298202414161816.jpg)
![Western Blot: beta-Catenin Antibody (A6-F8-R) [NBP3-32079] - Western blot analysis of beta-Catenin on different lysates with Mouse anti-beta-Catenin antibody (NBP3-32079) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: A431 cell lysate Lane 3: NCCIT cell lysate Lane 4: SW480 cell lysate Lane 5: HT-29 cell lysate Lane 6: HCT 116 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32079) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32079_mouse-beta-catenin-mab-a6-f8-r-278202415212464.jpg "Western Blot: beta-Catenin Antibody (A6-F8-R) [NBP3-32079] - Western blot analysis of beta-Catenin on different lysates with Mouse anti-beta-Catenin antibody (NBP3-32079) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: A431 cell lysate Lane 3: NCCIT cell lysate Lane 4: SW480 cell lysate Lane 5: HT-29 cell lysate Lane 6: HCT 116 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32079) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (A6-F8-R) [NBP3-32079] - Immunocytochemistry analysis of MCF7 cells labeling beta-Catenin with Mouse anti-beta-Catenin antibody (NBP3-32079) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-beta-Catenin antibody (NBP3-32079) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32079_mouse-beta-catenin-mab-a6-f8-r-29202410334026.jpg)
![Flow Cytometry: beta-Catenin Antibody (A6-F8-R) [NBP3-32079] - Flow cytometric analysis of MCF7 cells labeling beta-Catenin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32079, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 594 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32079_mouse-beta-catenin-mab-a6-f8-r-392024884635.jpg)
![Western Blot: CACNA1G Antibody [NBP1-80105] - Host: Rabbit. Target Name: CACNA1G. Sample Tissue: Human 786-0 Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/CACNA1G-Antibody-Western-Blot-NBP1-80105-img0005.jpg "Western Blot: CACNA1G Antibody [NBP1-80105] - Host: Rabbit. Target Name: CACNA1G. Sample Tissue: Human 786-0 Whole Cell. Antibody Dilution: 1ug/ml")
![Immunohistochemistry-Paraffin: CACNA1G Antibody [NBP1-80105] - Human Brain, cortex tissue at an antibody concentration of 2.5ug/ml.](http://images.novusbio.com/fullsize/CACNA1G-Antibody-Immunohistochemistry-Paraffin-NBP1-80105-img0004.jpg)
![Western Blot: CACNA1G Antibody [NBP1-80105] - Titration: 0.2-1 ug/ml, Positive Control: Human Spleen.](http://images.novusbio.com/fullsize/CACNA1G-Antibody-Western-Blot-NBP1-80105-img0001.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody (PSH03-47) [NBP3-32111] - Western blot analysis of Calcium-sensing R/CaSR on different lysates with Rabbit anti-Calcium-sensing R/CaSR antibody (NBP3-32111) at 1/2,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Mouse kidney tissue lysate (no heat) Lane 3: Rat kidney tissue lysate (no heat) Lysates/proteins at 40 ug/Lane. Predicted band size: 121 kDa Observed band size: 160 kDa Exposure time: 1 minute 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32111) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32111_rabbit-calcium-sensing-r-casr-mab-psh03-47-278202416331274.jpg "Western Blot: Calcium-sensing R/CaSR Antibody (PSH03-47) [NBP3-32111] - Western blot analysis of Calcium-sensing R/CaSR on different lysates with Rabbit anti-Calcium-sensing R/CaSR antibody (NBP3-32111) at 1/2,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Mouse kidney tissue lysate (no heat) Lane 3: Rat kidney tissue lysate (no heat) Lysates/proteins at 40 ug/Lane. Predicted band size: 121 kDa Observed band size: 160 kDa Exposure time: 1 minute 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32111) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Calcium-sensing R/CaSR Antibody (PSH03-47) [NBP3-32111] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Calcium-sensing R/CaSR antibody (NBP3-32111) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32111) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32111_rabbit-calcium-sensing-r-casr-mab-psh03-47-298202414333644.jpg)
![Immunohistochemistry: Calcium-sensing R/CaSR Antibody (PSH03-47) [NBP3-32111] - Immunofluorescence analysis of paraffin-embedded mouse kidney tissue labeling Calcium-sensing R/CaSR with Rabbit anti-Calcium-sensing R/CaSR antibody (NBP3-32111) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32111, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32111_rabbit-calcium-sensing-r-casr-mab-psh03-47-29820241492610.jpg)
![Flow Cytometry: Park7/DJ-1 Antibody (007) [NBP3-05805] - Flow cytometric analysis of Human Park7/DJ-1 expression on HeLa cells. The cells were treated, stained with NBP3-05805, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/nbp3-05805_rabbit-monoclonal-park7-dj-1-antibody-007-235202521451748.png "Flow Cytometry: Park7/DJ-1 Antibody (007) [NBP3-05805] - Flow cytometric analysis of Human Park7/DJ-1 expression on HeLa cells. The cells were treated, stained with NBP3-05805, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![Immunocytochemistry/ Immunofluorescence: Park7/DJ-1 Antibody (007) [NBP3-05805] - Immunofluorescence staining of Human Park7/DJ-1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP3-05805 (1:60) at 37 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp3-05805_rabbit-monoclonal-park7-dj-1-antibody-007-23520251628125.png)
![Western Blot: Park7/DJ-1 Antibody (007) [NBP3-05805] - NBP3-05805 at 1:1000 dilution Lane A: Hela Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:20 kDa Observed band size:24 kDa](http://images.novusbio.com/fullsize/nbp3-05805_rabbit-monoclonal-park7-dj-1-antibody-007-23520252135168.png)
![Immunoprecipitation: Park7/DJ-1 Antibody (007) [NBP3-05805] - PARK7 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate Lane B:0.5 mg Hela Whole Cell Lysate Lane C:0.5 mg 293T Whole Cell Lysate 2 uL NBP3-05805 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-05805, at 1:1000 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 20 kDa Observed band size: 20 kDa](http://images.novusbio.com/fullsize/nbp3-05805_rabbit-monoclonal-park7-dj-1-antibody-007-235202514331514.png)
![Western Blot: MAG/Siglec-4a Antibody (PSH02-41) [NBP3-32557] - Western blot analysis of MAG/Siglec-4a on different lysates with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32557) at 1/2,000 dilution. Lane 1: Mouse brain(no heat) tissue lysate Lane 2: Mouse cerebellum tissue lysate Lane 3: Mouse liver tissue lysate (negative) Lane 4: Rat brain(no heat) tissue lysate Lane 5: Rat cerebellum tissue lysate Lane 6: Rat liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 69 kDa Observed band size: 100 kDa Exposure time: 42 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32557) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32557_rabbit-mag-siglec-4a-mab-psh02-41-278202416285214.jpg "Western Blot: MAG/Siglec-4a Antibody (PSH02-41) [NBP3-32557] - Western blot analysis of MAG/Siglec-4a on different lysates with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32557) at 1/2,000 dilution. Lane 1: Mouse brain(no heat) tissue lysate Lane 2: Mouse cerebellum tissue lysate Lane 3: Mouse liver tissue lysate (negative) Lane 4: Rat brain(no heat) tissue lysate Lane 5: Rat cerebellum tissue lysate Lane 6: Rat liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 69 kDa Observed band size: 100 kDa Exposure time: 42 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32557) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MAG/Siglec-4a Antibody (PSH02-41) [NBP3-32557] - Immunofluorescence analysis of paraffin-embedded rat cerebellum tissue labeling MAG/Siglec-4a with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32557) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32557, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32557_rabbit-mag-siglec-4a-mab-psh02-41-29820241435217.jpg)
![Immunohistochemistry: MAG/Siglec-4a Antibody (PSH02-41) [NBP3-32557] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32557) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32557) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32557_rabbit-mag-siglec-4a-mab-psh02-41-298202414115423.jpg)
![Flow Cytometry: HDAC6 Antibody (159) [NBP3-09113] - Separation of K562 cells stained using anti-HDAC6 (159) purified antibody (concentration in sample 4,0 ug/ml, GAM APC, red-filled) from K562 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).](http://images.novusbio.com/fullsize/HDAC6-Antibody-159-Flow-Cytometry-NBP3-09113-img0001.jpg "Flow Cytometry: HDAC6 Antibody (159) [NBP3-09113] - Separation of K562 cells stained using anti-HDAC6 (159) purified antibody (concentration in sample 4,0 ug/ml, GAM APC, red-filled) from K562 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).")
![Western Blot: Ataxin-3 Antibody [NBP3-12686] - Lane A: U-87MG Whole Cell LysateLane B: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:42 kDaObserved band size:42 kDa](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Western-Blot-NBP3-12686-img0001.jpg "Western Blot: Ataxin-3 Antibody [NBP3-12686] - Lane A: U-87MG Whole Cell LysateLane B: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:42 kDaObserved band size:42 kDa")
![Immunohistochemistry-Paraffin: Ataxin-3 Antibody [NBP3-12686] - Staining of human ATXN3 in human testis with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Immunohistochemistry-Paraffin-NBP3-12686-img0002.jpg)
![Immunoprecipitation: Ataxin-3 Antibody [NBP3-12686] - Lane A:0.5 mg MCF7 Whole Cell Lysate4 uL anti-ATXN3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ATXN3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 42 kDaObserved band size :50 kDa](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Immunoprecipitation-NBP3-12686-img0003.jpg)
![Western Blot: HCN1 Antibody [NBP3-12563] - Lane A: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:99 kDaObserved band size:100 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/HCN1-Antibody-Western-Blot-NBP3-12563-img0001.jpg "Western Blot: HCN1 Antibody [NBP3-12563] - Lane A: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:99 kDaObserved band size:100 kDa(We are unsure as to the identity of these extra bands.)")
![Immunohistochemistry-Paraffin: HCN1 Antibody [NBP3-12563] - Staining of human HCN1 in human brain with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunohistochemistry-Paraffin-NBP3-12563-img0003.jpg)
![Immunoprecipitation: HCN1 Antibody [NBP3-12563] - Lane A:0.5 mg U-251 MG Whole Cell Lysate4 uL anti-HCN1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-HCN1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 99 kDaObserved band size :100 kDa](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunoprecipitation-NBP3-12563-img0002.jpg)