| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37261-0.025ml | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Atto594 | NBP3-14765-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-82375SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47950-0.1mg | |||
| IHC-Prediluted | NBP2-48191 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90209-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80782-25ul | |||
![Western Blot: BACE-1 Antibody (3C1C3) [NBP2-37261] - Analysis using BACE1 mouse mAb against Hela (1), SK-N-SH (2), HepG2 (3), C6 (4), PC-12 (5), PANC-1 (6), NIH/3T3 (7) cell lysate.](http://images.novusbio.com/fullsize/BACE-1-Antibody-3C1C3-Western-Blot-NBP2-37261-img0004.jpg "Western Blot: BACE-1 Antibody (3C1C3) [NBP2-37261] - Analysis using BACE1 mouse mAb against Hela (1), SK-N-SH (2), HepG2 (3), C6 (4), PC-12 (5), PANC-1 (6), NIH/3T3 (7) cell lysate.")
![Immunocytochemistry/Immunofluorescence: BACE-1 Antibody (3C1C3) [NBP2-37261] - Analysis of MCF-7 cells using BACE1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/BACE-1-Antibody-3C1C3-Immunocytochemistry-Immunofluorescence-NBP2-37261-img0005.jpg)
![Flow Cytometry: BACE-1 Antibody (3C1C3) [NBP2-37261] - Analysis of Hela cells using BACE1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/BACE-1-Antibody-3C1C3-Flow-Cytometry-NBP2-37261-img0006.jpg)
![Western Blot: BACE-1 Antibody (3C1C3) [NBP2-37261] - Analysis using BACE1 mAb against human BACE1 (AA: 112-324) recombinant protein. (Expected MW is 49.9 kDa)](http://images.novusbio.com/fullsize/BACE-1-Antibody-3C1C3-Western-Blot-NBP2-37261-img0002.jpg)
![Western Blot: BACE-1 Antibody (3C1C3) [NBP2-37261] - Analysis using BACE1 mAb against HEK293 (1) and BACE1 (AA: 112-324)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/BACE-1-Antibody-3C1C3-Western-Blot-NBP2-37261-img0003.jpg)
![ELISA: BACE-1 Antibody (3C1C3) [NBP2-37261] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/BACE-1-Antibody-3C1C3-ELISA-NBP2-37261-img0001.jpg)
![Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Active Alpha Synuclein Protein Aggregate.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Western-Blot-NBP2-54789-img0005.jpg "Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Active Alpha Synuclein Protein Aggregate.")
![Immunocytochemistry/Immunofluorescence: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Primary rat hippocampal neurons show lewy body inclusion formation when treated with active Alpha Synuclein Protein Aggregate at 4 ug/ml (D-F), but not when treated with control Alpha Synuclein Protein Aggregate at 4 ug/ml (A-C). Tissue: Primary hippocampal neurons. Species: Sprague-Dawley rat. Fixation: 4% formaldehyde from PFA. Primary Antibody: Mouse anti-pSer129 Antibody at 1:1000 24 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:700 for 1 hours at RT. Counterstain: Hoechst (blue) nuclear stain at 1:4000 for 1 hour at RT. Localization: Lewy body incluscions. Magnification: 20x.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Immunocytochemistry-Immunofluorescence-NBP2-54789-img0004.jpg)
![Immunohistochemistry: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Immunohistochemistry analysis of rat brain injected with Type 1 human alpha synuclein PFFs (NBP2-54789). Species: Female Sprague-Dawley Rat. Rat was injected with 16g Type 1 human alpha synuclein PFFs (NBP2-54789) in each of 2 injection sites: AP+1.6, ML+2.4, DV-4.2 from skull; and AP-1.4, ML+0.2, DV-2.8 from skull. 30-days post-injection. Fixation: Saline perfusion followed by 4% PFA fixation for 48 hrs. Primary antibody: rabbit monoclonal anti-pSer129 alpha synuclein. Secondary Antibody: Biotin-SP Donkey Anti-Rabbit IgG (H+L) at 1:500 for 2 hours in cold room with shaking. ABC signal amplification, DAB staining. Magnification: 20X. Alpha synuclein pathology is seen in the striatum close to an injection site.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Immunohistochemistry-NBP2-54789-img0007.jpg)
![In vitro assay: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate (NBP2-54789) is combined with 100 uM of active alpha Synuclein monomer (NBP2-54788), as compared to active alpha Synuclein aggregate (NBP2-54789) and active alpha Synuclein monomer (NBP2-54788) alone. Thioflavin T ex = 450 nm, em = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-In-vitro-assay-NBP2-54789-img0006.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - TEM of Type 1 alpha-Synuclein Pre-formed Fibrils (PFFs) (NBP2-54789)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-54789-img0008.jpg)
![Product Image: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Toxicity results comparing Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 2) and Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 1) (Catalog No. NBP2-54789). Data was graphed after live cell imaging results were obtained using the following procedure: After 8 days in vitro, primary rat mixed cortical neuron cells were washed with 1X PBS and treated with 500 g/ml of Type 1 and Type 2 alpha-Synuclein Proteins for 20 hours at 37C. Following treatements, cells were washed with 2X PBS and incubated with a staining solution (2.0 M Cell Event + 2.5 M Ethidium homodimer + 2.5 g/ml Hoechst 33342 in sterile HBSS) for 30 minutes at 37C. The addition of the Type 2 alpha-Synuclein Proteins resulted in a significant increase in cell death.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Product-Image-NBP2-54789-img0010.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - TEM of Type 1 alpha-Synuclein Pre-formed Fibrils (PFFs) (NBP2-54789)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-54789-img0009.jpg)
![Western Blot: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta-Catenin.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Western-Blot-NBP2-70508-img0002.jpg "Western Blot: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta-Catenin.")
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY beta-Catenin.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70508-img0008.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70508-img0007.jpg)
![Western Blot: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Western-Blot-NBP2-70508-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70508-img0009.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70508-img0003.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70508-img0004.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70508-img0005.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70508-img0006.jpg)
![Western Blot: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of Beta-Catenin on HT-29 cell lysates with Rabbit anti-Beta-Catenin antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-SA30-04-Western-Blot-NBP2-67307-img0009.jpg "Western Blot: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of Beta-Catenin on HT-29 cell lysates with Rabbit anti-Beta-Catenin antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Usage of Beta Catenin antibody in ICC of HT-29 cells](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-SA30-04-Immunocytochemistry-NBP2-67307-img0004.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Immunofluorescence analysis of paraffin-embedded human colon carcinoma tissue labeling Beta-Catenin with Rabbit anti-Beta-Catenin antibody washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-SA30-04-Immunohistochemistry-Paraffin-NBP2-67307-img0010.jpg)
![Western Blot: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of Beta-Catenin on different cell lysates using anti- Beta-Catenin antibody at 1/1,000 dilution. Positive control: Lane 1: SW480 Lane 2: HT29 Lane 3: A431](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-SA30-04-Western-Blot-NBP2-67307-img0007.jpg)
![Western Blot: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of Beta-Catenin on different lysates with Rabbit anti-Beta-Catenin antibody at 1/500 dilution. Lane 1: SW480 cell lysate Lane 2: A431 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-SA30-04-Western-Blot-NBP2-67307-img0008.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of mouse small intestine tissue using anti-b-Catenin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-SA30-04-Immunohistochemistry-Paraffin-NBP2-67307-img0002.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of mouse colon tissue using anti- Beta-Catenin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-SA30-04-Immunohistochemistry-Paraffin-NBP2-67307-img0005.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of human colon cancer tissue using anti- Beta-Catenin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-SA30-04-Immunohistochemistry-Paraffin-NBP2-67307-img0006.jpg)
![Western Blot: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of STUB1 on different lysates using anti-STUB1 antibody at 1/2,000 dilution.Positive control:Lane 1: 293 Lane 2: MCF-7Lane 3: SK-Br-3 Lane 4: Mouse kidney](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Western-Blot-NBP2-75440-img0009.jpg "Western Blot: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of STUB1 on different lysates using anti-STUB1 antibody at 1/2,000 dilution.Positive control:Lane 1: 293 Lane 2: MCF-7Lane 3: SK-Br-3 Lane 4: Mouse kidney")
![Immunocytochemistry/Immunofluorescence: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Staining STUB1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Immunocytochemistry-Immunofluorescence-NBP2-75440-img0004.jpg)
![Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of paraffin-embedded rat epididymis tissue using anti-STUB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Immunohistochemistry-Paraffin-NBP2-75440-img0008.jpg)
![Flow Cytometry: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of SH-SY-5Y cells with STUB1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Flow-Cytometry-NBP2-75440-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Staining STUB1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Immunocytochemistry-Immunofluorescence-NBP2-75440-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Staining STUB1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Immunocytochemistry-Immunofluorescence-NBP2-75440-img0003.jpg)
![Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of paraffin-embedded human colon cancer tissue using anti-STUB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Immunohistochemistry-Paraffin-NBP2-75440-img0005.jpg)
![Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of paraffin-embedded human placenta tissue using anti-STUB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Immunohistochemistry-Paraffin-NBP2-75440-img0006.jpg)
![Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of paraffin-embedded mouse kidney tissue using anti-STUB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Immunohistochemistry-Paraffin-NBP2-75440-img0007.jpg)
![Western Blot: Mucolipin 1 Antibody [NB110-82375] - Total protein from mouse brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Mucolipin 1 (NB110-82375) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/Mucolipin-1-Antibody-Western-Blot-NB110-82375-img0005.jpg "Western Blot: Mucolipin 1 Antibody [NB110-82375] - Total protein from mouse brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Mucolipin 1 (NB110-82375) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Simple Western: Mucolipin 1 Antibody [NB110-82375] - Lane view shows a specific band for Mucolipin 1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Mucolipin-1-Antibody-Simple-Western-NB110-82375-img0004.jpg)
![Western Blot: RPSA Antibody [NBP1-33002] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RPSA antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/RPSA-Antibody-Western-Blot-NBP1-33002-img0018.jpg "Western Blot: RPSA Antibody [NBP1-33002] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RPSA antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: RPSA Antibody [NBP1-33002] - CA922 xenograft, using RPSA antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RPSA-Antibody-Immunohistochemistry-Paraffin-NBP1-33002-img0012.jpg)
![Western Blot: RPSA Antibody [NBP1-33002] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/RPSA-Antibody-Western-Blot-NBP1-33002-img0013.jpg)
![Western Blot: RPSA Antibody [NBP1-33002] - Whole cell extracts (30 ug) was separated by 10 % SDS-PAGE, and blotted with RPSA antibody diluted by 1:1000](http://images.novusbio.com/fullsize/RPSA-Antibody-Western-Blot-NBP1-33002-img0014.jpg)
![Western Blot: RPSA Antibody [NBP1-33002] - Liver protein expression in different experimental groups of Laminin R, AdipoR2 and SIRT1.Western Blotting analysis of protein expression in the liver on different experimental groups of aminin Receptor. Image shows demonstrative bands of the analyzed proteins and respective housekeeping protein (beta-tubulin) in liver. Data are expressed in mean +/- s.e.m. *p<0.05 Control diet and EGCG (CE) group versus Control diet and Water (CW) group. #p<0.05 High-fat diet and EGCG (HFE) group versus HFW group. $p<0.05 HFW versus CW. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0141227), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RPSA-Antibody-Western-Blot-NBP1-33002-img0015.jpg)
![Western Blot: RPSA Antibody [NBP1-33002] - Sample (30 ug of whole cell lysate). A: Jurkat. 12% SDS PAGE. RPSA Antibody diluted at 1:1000](http://images.novusbio.com/fullsize/RPSA-Antibody-Western-Blot-NBP1-33002-img0016.jpg)
![Western Blot: RPSA Antibody [NBP1-33002] - RPSA antibody detects RPSA protein by Western blot analysis. Mouse tissue extracts (50 ug) was separated by 10 % SDS-PAGE, and the membrane was blotted with RPSA antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/RPSA-Antibody-Western-Blot-NBP1-33002-img0017.jpg)
![Immunohistochemistry-Paraffin: RPSA Antibody [NBP1-33002] - Mouse small intestine. RPSA antibody [N1C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RPSA-Antibody-Immunohistochemistry-Paraffin-NBP1-33002-img0009.jpg)
![Western Blot: RPSA Antibody [NBP1-33002] - RPSA antibody detects RPSA protein by Western blot analysis. Whole cell extracts (30 ug) was separated by 10 % SDS-PAGE, and blotted with RPSA antibody (NBP1-33002) diluted by 1:1000](http://images.novusbio.com/fullsize/nbp1-33002_rabbit-polyclonal-rpsa-antibody-9102024205675.jpg)
![Western Blot: Vimentin Antibody (VM1170) [NBP2-44833] - Western Blot Analysis of HEP2 cell lysate using Vimentin Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Western-Blot-NBP2-44833-img0008.jpg "Western Blot: Vimentin Antibody (VM1170) [NBP2-44833] - Western Blot Analysis of HEP2 cell lysate using Vimentin Antibody (VM1170).")
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Vimentin Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Immunohistochemistry-Paraffin-NBP2-44833-img0007.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Human Angiosarcoma stained with Vimentin Monoclonal Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Immunohistochemistry-Paraffin-NBP2-44833-img0001.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Human Ewing's Sarcoma stained with Vimentin Monoclonal Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Immunohistochemistry-Paraffin-NBP2-44833-img0002.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Human Leiomyosarcoma stained with Vimentin Monoclonal Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Immunohistochemistry-Paraffin-NBP2-44833-img0003.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Human Melanoma stained with Vimentin Monoclonal Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Immunohistochemistry-Paraffin-NBP2-44833-img0004.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Human Rhabdomyosarcoma stained with Vimentin Monoclonal Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Immunohistochemistry-Paraffin-NBP2-44833-img0005.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Human Uterus stained with Vimentin Monoclonal Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Immunohistochemistry-Paraffin-NBP2-44833-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Agrin Antibody [NBP1-90209] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Agrin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90209-img0007.jpg "Immunocytochemistry/Immunofluorescence: Agrin Antibody [NBP1-90209] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Agrin Antibody [NBP1-90209] - Staining of human Fallopian tube shows moderate positivity in basement membrane in glandular cells.](http://images.novusbio.com/fullsize/Agrin-Antibody-Immunohistochemistry-Paraffin-NBP1-90209-img0015.jpg)
![Immunohistochemistry-Paraffin: Agrin Antibody [NBP1-90209] - Staining of human lung shows moderate membranous positivity in pneumocytes.](http://images.novusbio.com/fullsize/Agrin-Antibody-Immunohistochemistry-Paraffin-NBP1-90209-img0013.jpg)
![Immunohistochemistry-Paraffin: Agrin Antibody [NBP1-90209] - Staining of human kidney shows weak membranous positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/Agrin-Antibody-Immunohistochemistry-Paraffin-NBP1-90209-img0014.jpg)
![Immunohistochemistry-Paraffin: Agrin Antibody [NBP1-90209] - Staining of human colon shows strong membranous positivity in endothelial cells.](http://images.novusbio.com/fullsize/Agrin-Antibody-Immunohistochemistry-Paraffin-NBP1-90209-img0016.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0021.jpg "Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.")
![Immunocytochemistry/Immunofluorescence: beta Amyloid Antibody [NBP1-78007]](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78007-img0015.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Human Heart (formalin-fixed, paraffin-embedded) stained with beta Amyloid antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0024.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Mouse Brain (Lane 1) and Mouse Spinal Chord (Lane 2) were run on a 4-20% gradient gel, Blocked in 1% BSA-TBS-T 30 min RT and probed with Rb-a-Beta Amyloid 1:1000 in 1% BSA-TBS-T o/n 4C. Note: observed band higher than predicted molecular weight of 5 kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0010.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Lane 1: HEK293 WCL. Lane 2: Mouse Brain WCL. Lane 3: A-172 WCL. Load: 10.0ug per lane. Primary antibody: Beta Amyloid Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase Conjugated Goat Anti Rabbit IgG at 1:40,000 for 30 min at RT. Block for 30 min at RT. Note: observed band higher than predicted molecular weight of 5 kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0018.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate. Lane 2: HeLa Lysate.Lane 3: MCF-7 Lysate.Lane 4: Jurkat Lysate.Lane 5: A431 Lysate.Lane 6: LNCaP Lysate.Lane 7: A-172 Lysate.Lane 8: NIH/3T3 Lysate. Load: 35 ug per lane.Primary antibody: beta Amyloid antibody at 1:5,000 for overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT.Blocking Buffer: 1% Casein-TTBS for 30 min at RT.Predicted MW: ~40-50 kDa. Observed size: ~48 kDa for beta Amyloid.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0019.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Lane 5: Daudi Whole Cell Lysate. Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0020.jpg)
![Immunohistochemistry-Paraffin: beta Amyloid Antibody [NBP1-78007] - Human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear and cytoplasmic staining between individual tumors, with some tumors showing moderate staining. This image shows EGR-1 staining of human ovarian carcinoma.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-Paraffin-NBP1-78007-img0008.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Immunohistochemistical detection of beta Amyloid using beta Amyloid antibody on TG APP23 mouse brain cortex frozen sections. beta Amyloid antibody used at 1/200 and incubated for 2 hours in TBS/BSA/Tween/azide. Fluorescent labelled anti rabbit IgG was then added. Carl Hobbs, King`s College London, United Kingdom](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0022.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Immunohistochemistry with beta Amyloid antibody showing amyloid beta plaque staining in human Alzheimer’s disease brain at 10x and 20x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval with E1 (Leica Microsystems) retrieval solution for 20 min and then incubated with rabbit beta Amyloid antibody at 1:100 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3’3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0023.jpg)
![ELISA: beta Amyloid Antibody [NBP1-78007] - ELISA results of purified beta Amyloid antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-ELISA-NBP1-78007-img0025.jpg)
![Western Blot: VAMP-2 Antibody [NBP3-13179] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with VAMP-2 antibody (NBP3-13179) diluted at 1:1000.](http://images.novusbio.com/fullsize/VAMP-2-Antibody-Western-Blot-NBP3-13179-img0001.jpg "Western Blot: VAMP-2 Antibody [NBP3-13179] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with VAMP-2 antibody (NBP3-13179) diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: VAMP-2 Antibody [NBP3-13179] - VAMP-2 antibody detects VAMP-2 protein at synaptic vesicles by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: VAMP-2 protein stained by VAMP-2 antibody (NBP3-13179) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue:Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/VAMP-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13179-img0006.jpg)
![Immunohistochemistry-Paraffin: VAMP-2 Antibody [NBP3-13179] - VAMP-2 antibody detects VAMP-2 protein expression by immunohistochemical analysis. Sample:Paraffin-Embedded adult mouse retina. Green: VAMP-2 protein stained by VAMP-2 antibody (NBP3-13179) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/VAMP-2-Antibody-Immunohistochemistry-Paraffin-NBP3-13179-img0007.jpg)
![Immunohistochemistry-Frozen: VAMP-2 Antibody [NBP3-13179] - VAMP-2 antibody detects VAMP-2 protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: VAMP-2 protein stained by VAMP-2 antibody (NBP3-13179) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/VAMP-2-Antibody-Immunohistochemistry-Frozen-NBP3-13179-img0002.jpg)
![Immunohistochemistry-Paraffin: VAMP-2 Antibody [NBP3-13179] - VAMP-2 antibody detects VAMP-2 protein at cell membrane in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. VAMP-2 antibody (NBP3-13179) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/VAMP-2-Antibody-Immunohistochemistry-Paraffin-NBP3-13179-img0003.jpg)
![Immunohistochemistry-Paraffin: VAMP-2 Antibody [NBP3-13179] - VAMP-2 antibody detects VAMP-2 protein at cell membrane in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. VAMP-2 antibody (NBP3-13179) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/VAMP-2-Antibody-Immunohistochemistry-Paraffin-NBP3-13179-img0004.jpg)
![Immunohistochemistry-Paraffin: VAMP-2 Antibody [NBP3-13179] - VAMP-2 antibody detects VAMP-2 protein at cell membrane in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. VAMP-2 antibody (NBP3-13179) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/VAMP-2-Antibody-Immunohistochemistry-Paraffin-NBP3-13179-img0005.jpg)
![Immunohistochemistry-Paraffin: Chromogranin B Antibody [NBP1-80782] - Analysis in human adrenal gland and liver tissues using NBP1-80782 antibody. Corresponding CHGB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Chromogranin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-80782-img0024.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Chromogranin B Antibody [NBP1-80782] - Analysis in human adrenal gland and liver tissues using NBP1-80782 antibody. Corresponding CHGB RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Chromogranin B Antibody [NBP1-80782] - Staining of human adrenal gland, colon, liver and pancreas using Anti-CHGB antibody NBP1-80782 (A) shows similar protein distribution across tissues to independent antibody NBP1-80781 (B).](http://images.novusbio.com/fullsize/Chromogranin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-80782-img0017.jpg)
![Western Blot: Chromogranin B Antibody [NBP1-80782] - Analysis in human cell line SH-SY5Y.](http://images.novusbio.com/fullsize/Chromogranin-B-Antibody-Western-Blot-NBP1-80782-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: Chromogranin B Antibody [NBP1-80782] - Staining of human cell line SH-SY5Y shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Chromogranin-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80782-img0006.jpg)
![Immunohistochemistry-Paraffin: Chromogranin B Antibody [NBP1-80782] - Staining of human pancreas shows strong cytoplasmic positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/Chromogranin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-80782-img0018.jpg)
![Immunohistochemistry-Paraffin: Chromogranin B Antibody [NBP1-80782] - Staining of human colon shows strong cytoplasmic positivity in enteroendocrine cells.](http://images.novusbio.com/fullsize/Chromogranin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-80782-img0019.jpg)
![Immunohistochemistry-Paraffin: Chromogranin B Antibody [NBP1-80782] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Chromogranin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-80782-img0020.jpg)