| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99063-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97814-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97747-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97569-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09006-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB17572-SP | |||
![Western Blot: HDAC6 Antibody (236) [NBP3-05295] - Western blotting analysis of human HDAC6 by mouse monoclonal antibody NBP3-05295 in HEK-293T cell line under reducing conditions. PVDF membrane was blocked in 5% milk, and incubated with primary antibody (1 ug/ml), followed by HRP-conjugated anti-mouse secondary antibody.](http://images.novusbio.com/fullsize/HDAC6-Antibody-236-Western-Blot-NBP3-05295-img0004.jpg "Western Blot: HDAC6 Antibody (236) [NBP3-05295] - Western blotting analysis of human HDAC6 by mouse monoclonal antibody NBP3-05295 in HEK-293T cell line under reducing conditions. PVDF membrane was blocked in 5% milk, and incubated with primary antibody (1 ug/ml), followed by HRP-conjugated anti-mouse secondary antibody.")
![Immunocytochemistry/Immunofluorescence: HDAC6 Antibody (236) [NBP3-05295] - Immunocytochemistry staining of HDAC6 in formaldehyde-fixed and Triton-permeabilized HEK-293T cells (A) and SH-SY5Y cells (B) by mouse monoclonal antibody NBP3-05295, followed by anti-mouse Alexa Fluor 488 (green), DNA indicated by DAPI (blue). In SH-SY5Y cells tubulin was stained by a rabbit polyclonal antibody, followed by anti-rabbit Alexa Fluor 647 (red, colocalization yellow).](http://images.novusbio.com/fullsize/HDAC6-Antibody-236-Immunocytochemistry-Immunofluorescence-NBP3-05295-img0001.jpg)
![ELISA: HDAC6 Antibody (236) [NBP3-05295] - ELISA analysis of human HDAC6 by mouse monoclonal antibody NBP3-05295. Plate was coated with recombinant human HDAC6, then blocked with BSA, and exposed to dilution series of anti-HDAC6 primary antibody, followed by HRP-conjugated anti-mouse secondary antibody, and colorimetric signal of processed OPD substrate was measured at 492 nm.](http://images.novusbio.com/fullsize/HDAC6-Antibody-236-ELISA-NBP3-05295-img0002.jpg)
![Immunoprecipitation: HDAC6 Antibody (236) [NBP3-05295] - Immunoprecipitation of recombinant human HDAC6 by mouse monoclonal antibodies 178 and NBP3-05295 using protein G-coated Dynabeads. Eluted proteins were processed by SDS PAAGE under reducing conditions and visualized by Coomassie Brilliant Blue.](http://images.novusbio.com/fullsize/HDAC6-Antibody-236-Immunoprecipitation-NBP3-05295-img0003.jpg)
![Flow Cytometry: Mouse Monoclonal HDAC6 Antibody (236) [NBP3-05295] - Separation of K562 cells stained using anti-human HDAC6 (236) purified antibody (concentration in sample 5.33 ug/ml, GAM APC, red-filled) from K562 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).](http://images.novusbio.com/fullsize/antibody/nbp3-05295_mouse-monoclonal-hdac6-antibody-236-flow-cytometry-1112202412584..jpg)
![Immunohistochemistry: Somatostatin R1/SSTR1 Antibody [NBP3-14480] - Immunohistochemistry of ovary carcinoma](http://images.novusbio.com/fullsize/Somatostatin-R1-SSTR1-Antibody-Immunohistochemistry-NBP3-14480-img0006.jpg "Immunohistochemistry: Somatostatin R1/SSTR1 Antibody [NBP3-14480] - Immunohistochemistry of ovary carcinoma")
![Immunohistochemistry-Paraffin: Somatostatin R1/SSTR1 Antibody [NBP3-14480] - Immunohistochemistry of formalin-fixed, paraffin-embedded human pancreas, islet of Langerhans, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Somatostatin-R1-SSTR1-Antibody-Immunohistochemistry-Paraffin-NBP3-14480-img0001.jpg)
![Immunohistochemistry-Paraffin: Somatostatin R1/SSTR1 Antibody [NBP3-14480] - Immunohistochemistry of formalin-fixed, paraffin-embedded human lung, small cell carcinoma, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Somatostatin-R1-SSTR1-Antibody-Immunohistochemistry-Paraffin-NBP3-14480-img0002.jpg)
![Immunohistochemistry-Paraffin: Somatostatin R1/SSTR1 Antibody [NBP3-14480] - Immunohistochemistry of formalin-fixed, paraffin-embedded human prostate carcinoma after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Somatostatin-R1-SSTR1-Antibody-Immunohistochemistry-Paraffin-NBP3-14480-img0003.jpg)
![Immunohistochemistry-Paraffin: Somatostatin R1/SSTR1 Antibody [NBP3-14480] - Immunohistochemistry of formalin-fixed, paraffin-embedded human skin melanoma after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Somatostatin-R1-SSTR1-Antibody-Immunohistochemistry-Paraffin-NBP3-14480-img0004.jpg)
![Immunohistochemistry: Somatostatin R1/SSTR1 Antibody [NBP3-14480] - Immunohistochemistry of breast carcinoma](http://images.novusbio.com/fullsize/Somatostatin-R1-SSTR1-Antibody-Immunohistochemistry-NBP3-14480-img0005.jpg)
![Immunohistochemistry: Somatostatin R1/SSTR1 Antibody [NBP3-14480] - Immunohistochemistry of pancreas carcinoma](http://images.novusbio.com/fullsize/Somatostatin R1-SSTR1 Antibody-Immunohistochemistry-NBP3-14480-img0007.jpg)
![Western Blot: Calbindin D-28K Antibody [NBP2-99063] - Anti-Calbindin D-28K rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse kidney tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 27 kDa](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Western-Blot-NBP2-99063-img0003.jpg "Western Blot: Calbindin D-28K Antibody [NBP2-99063] - Anti-Calbindin D-28K rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse kidney tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 27 kDa")
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP2-99063] - Immunochemical staining of human Calbindin D-28K in human brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-Paraffin-NBP2-99063-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody [NBP2-99063] - Immunofluorescence staining of Calbindin D-28K in 293 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-99063 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-99063_rabbit-polyclonal-calbindin-d-28k-antibody-235202514331592.png)
![Immunoprecipitation: Calbindin D-28K Antibody [NBP2-99063] - Calbindin D-28K was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate. 4 ul NBP2-99063 and 60 ug of Immunomagnetic beads. Protein G. Primary antibody: NBP2-99063, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa](http://images.novusbio.com/fullsize/nbp2-99063_rabbit-polyclonal-calbindin-d-28k-antibody-235202516335024.png)
![Western Blot: WWOX Antibody [NBP2-97814] - Anti-WWOX rabbit polyclonal antibody at 1:500 dilution. Lane A: Raji Whole Cell Lysate Lane B: Mouse brain tissue lysate Lane C: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 47 kDa](http://images.novusbio.com/fullsize/WWOX-Antibody-Western-Blot-NBP2-97814-img0003.jpg "Western Blot: WWOX Antibody [NBP2-97814] - Anti-WWOX rabbit polyclonal antibody at 1:500 dilution. Lane A: Raji Whole Cell Lysate Lane B: Mouse brain tissue lysate Lane C: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 47 kDa")
![Immunohistochemistry-Paraffin: WWOX Antibody [NBP2-97814] - Immunochemical staining of human WWOX in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-Paraffin-NBP2-97814-img0004.jpg)
![Immunoprecipitation: WWOX Antibody [NBP2-97814] - WWOX was immunoprecipitated using: Lane A: 0.5 mg U251MG Whole Cell Lysate 4 ul anti-WWOX rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-WWOX rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 43 kDa](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunoprecipitation-NBP2-97814-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: WWOX Antibody [NBP2-97814] - Immunofluorescence staining of WWOX in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97814 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97814_rabbit-polyclonal-wwox-antibody-235202521451771.png)
![Western Blot: ADNP Antibody [NBP2-97747] - Anti-ADNP rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane.](http://images.novusbio.com/fullsize/ADNP-Antibody-Western-Blot-NBP2-97747-img0003.jpg "Western Blot: ADNP Antibody [NBP2-97747] - Anti-ADNP rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane.")
![Immunocytochemistry/Immunofluorescence: ADNP Antibody [NBP2-97747] - Immunofluorescence staining of ADNP in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human ADNP polyclonal antibody (dilution ratio 1:300) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG Secondary antibody (red). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/ADNP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97747-img0001.jpg)
![Immunoprecipitation: ADNP Antibody [NBP2-97747] - ADNP was immunoprecipitated using: Lane A: 0.5 mg U-251MG Whole Cell Lysate. 4 ul anti-ADNP rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-ADNP rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 122 kDa. Observed band size: 150 kDa.](http://images.novusbio.com/fullsize/ADNP-Antibody-Immunoprecipitation-NBP2-97747-img0004.jpg)
![Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Sodium Potassium ATPase Alpha 1 with Rabbit anti-Sodium Potassium ATPase Alpha 1 antibody (NBP3-33143) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33143, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33143_rabbit-sodium-potassium-atpase-alpha-1-mab-st0533-29820241420955.jpg "Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Sodium Potassium ATPase Alpha 1 with Rabbit anti-Sodium Potassium ATPase Alpha 1 antibody (NBP3-33143) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33143, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Sodium Potassium ATPase Alpha 1 antibody (NBP3-33143) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33143) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33143_rabbit-sodium-potassium-atpase-alpha-1-mab-st0533-298202413585572.jpg)
![Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Immunocytochemistry analysis of HeLa cells labeling Sodium Potassium ATPase Alpha 1 with Rabbit anti-Sodium Potassium ATPase Alpha 1 Alpha 1 antibody (NBP3-33143) at 1/500 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Sodium Potassium ATPase antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33143_rabbit-sodium-potassium-atpase-alpha-1-mab-st0533-29202410234287.jpg)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Western blot analysis of Sodium Potassium ATPase Alpha 1 on different lysates with Rabbit anti-Sodium Potassium ATPase Alpha 1 antibody (NBP3-33143) at 1/100,000 dilution. Lane 1: HeLa cell lysate (no heat) (20 µg/Lane) Lane 2: HT-29 cell lysate (no heat) (20 µg/Lane) Lane 3: HepG2 cell lysate (no heat) (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (no heat) (20 µg/Lane) Lane 5: L-929 cell lysate (no heat) (20 µg/Lane) Lane 6: Mouse brain tissue lysate (no heat) (20 µg/Lane) Lane 7: Rat brain tissue lysate (no heat) (20 µg/Lane) Predicted band size: 113 kDa Observed band size: 100 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/100,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33143_rabbit-sodium-potassium-atpase-alpha-1-mab-st0533-392024848504.jpg)
![Western Blot: SSR3 Antibody [NBP2-97569] - Lane A: A431 Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: U87 MG Whole Cell LysateLane D: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:21 kDaObserved band size:20 kDa](http://images.novusbio.com/fullsize/SSR3-Antibody-Western-Blot-NBP2-97569-img0003.jpg "Western Blot: SSR3 Antibody [NBP2-97569] - Lane A: A431 Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: U87 MG Whole Cell LysateLane D: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:21 kDaObserved band size:20 kDa")
![Immunoprecipitation: SSR3 Antibody [NBP2-97569] - Lane A:0.5 mg A431 Whole Cell Lysate4 uL anti-SSR3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-SSR3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 21 kDaObserved band size :21 kDa](http://images.novusbio.com/fullsize/SSR3-Antibody-Immunoprecipitation-NBP2-97569-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SSR3 Antibody [NBP2-97569] - Immunofluorescence staining of SSR3 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97569 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97569_rabbit-polyclonal-ssr3-antibody-23520251125449.png)
![Immunohistochemistry-Paraffin: SSR3 Antibody - BSA Free [NBP2-97569] - Immunochemical staining of human SSR3 in human brain with NBP2-97569 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp2-97569_rabbit-polyclonal-ssr3-antibody-23520251664760.png)
![Western Blot: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - HeLa (A), HEK293 (B), and HepG2 (C) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of F2C (NBP3-09006) at 0.01 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for alpha Tubulin is ~55kDa. NBP3-09006 successfully detected human alpha Tubulin in HeLa, HEK293, and HepG2 cell lysate samples.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Western-Blot-NBP3-09006-img0002.jpg "Western Blot: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - HeLa (A), HEK293 (B), and HepG2 (C) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of F2C (NBP3-09006) at 0.01 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for alpha Tubulin is ~55kDa. NBP3-09006 successfully detected human alpha Tubulin in HeLa, HEK293, and HepG2 cell lysate samples.")
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of F2C (NBP3-09006) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (1 ug/ml), showing microtubule staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09006, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09006-img0004.jpg)
![Immunohistochemistry-Paraffin: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - Anti-Alpha Tubulin staining of paraffin embedded human cerebral cortex tissue using the rabbit-chimeric version of F2C (NBP3-09006). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP3-09006 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of axons and a few neuronal cell bodies may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Immunohistochemistry-Paraffin-NBP3-09006-img0001.jpg)
![Flow Cytometry: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - HepG2 (A), HeLa (B) and Hek293 (C) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of F2C (NBP3-09006, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Flow-Cytometry-NBP3-09006-img0003.jpg)
![Western Blot: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43) [NBP2-79822] - Western Blot analysis of Human Hep G2 Hepatoblastoma Cell lysate showing detection of TR alpha/NR1A1/Thyroid Hormone Receptor alpha protein using Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody, Clone H43 (NBP2-79822). Load: 10 ug. Primary Antibody: Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody (NBP2-79822) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Other Band(s): Higher molceular weight bands could be due to PTMs.](http://images.novusbio.com/fullsize/TR-alpha-NR1A1-Thyroid-Hormone-Receptor-alpha-Antibody-H43-Western-Blot-NBP2-79822-img0002.jpg "Western Blot: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43) [NBP2-79822] - Western Blot analysis of Human Hep G2 Hepatoblastoma Cell lysate showing detection of TR alpha/NR1A1/Thyroid Hormone Receptor alpha protein using Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody, Clone H43 (NBP2-79822). Load: 10 ug. Primary Antibody: Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody (NBP2-79822) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Other Band(s): Higher molceular weight bands could be due to PTMs.")
![Immunocytochemistry/Immunofluorescence: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43) [NBP2-79822] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody, Clone H43 (NBP2-79822). Tissue: Differentiated SH-SY5Y. Species: Human. Primary Antibody: Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody (NBP2-79822) at 1:250. Secondary Antibody: AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue). (A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody. (D) Hoechst (E) Composite.](http://images.novusbio.com/fullsize/TR-alpha-NR1A1-Thyroid-Hormone-Receptor-alpha-Antibody-H43-Immunocytochemistry-Immunofluorescence-NBP2-79822-img0005.jpg)
![Immunohistochemistry: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43) [NBP2-79822] - Immunohistochemistry analysis using Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody, Clone H43 (NBP2-79822). Tissue: Thyroid Cancer. Species: Human. Primary Antibody: Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody (NBP2-79822) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 4X.](http://images.novusbio.com/fullsize/TR-alpha-NR1A1-Thyroid-Hormone-Receptor-alpha-Antibody-H43-Immunohistochemistry-NBP2-79822-img0004.jpg)
![Immunohistochemistry: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43) [NBP2-79822] - Immunohistochemistry analysis using Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody, Clone H43 (NBP2-79822). Tissue: Thyroid. Species: Mouse. Primary Antibody: Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody (NBP2-79822) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 7.5X.](http://images.novusbio.com/fullsize/TR-alpha-NR1A1-Thyroid-Hormone-Receptor-alpha-Antibody-H43-Immunohistochemistry-NBP2-79822-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: CCR5 Antibody (12D1) - Azide and BSA Free [NBP2-47759] - Immunofluorescence staining of PFA-fixed HeLa cells using CCR5 Mouse Monoclonal Antibody (12D1) followed by goat anti-mouse IgG-CF488 (green).](http://images.novusbio.com/fullsize/CCR5-Antibody-12D1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-47759-img0002.jpg "Immunocytochemistry/Immunofluorescence: CCR5 Antibody (12D1) - Azide and BSA Free [NBP2-47759] - Immunofluorescence staining of PFA-fixed HeLa cells using CCR5 Mouse Monoclonal Antibody (12D1) followed by goat anti-mouse IgG-CF488 (green).")
![Immunohistochemistry-Paraffin: CCR5 Antibody (12D1) - Azide and BSA Free [NBP2-47759] - Human Stomach tissue stained with CCR5 Antibody (12D1).](http://images.novusbio.com/fullsize/CCR5-Antibody-12D1-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47759-img0001.jpg)
![Flow Cytometry: CCR5 Antibody (12D1) - Azide and BSA Free [NBP2-47759] - Flow Cytometric Analysis of MCF-7 cells using CCR5 Mouse Monoclonal Antibody (12D1) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CCR5-Antibody-12D1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-47759-img0003.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/1507) - Azide and BSA Free [NBP2-54542] - beta-Catenin Antibody (CTNNB1/1507) [NBP2-54542] - Formalin-fixed, paraffin-embedded Mouse Colon stained with Beta-Catenin Monoclonal Antibody (CTNNB1/1507).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CTNNB1-1507-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54542-img0008.jpg "Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/1507) - Azide and BSA Free [NBP2-54542] - beta-Catenin Antibody (CTNNB1/1507) [NBP2-54542] - Formalin-fixed, paraffin-embedded Mouse Colon stained with Beta-Catenin Monoclonal Antibody (CTNNB1/1507).")
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/1507) - Azide and BSA Free [NBP2-54542] - beta-Catenin Antibody (CTNNB1/1507) [NBP2-54542] - Formalin-fixed, paraffin-embedded human Pancreas stained with Beta-Catenin Monoclonal Antibody (CTNNB1/1507).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CTNNB1-1507-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54542-img0005.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/1507) - Azide and BSA Free [NBP2-54542] - beta-Catenin Antibody (CTNNB1/1507) [NBP2-54542] - Formalin-fixed, paraffin-embedded human Tonsil stained with Beta-Catenin Monoclonal Antibody (CTNNB1/1507).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CTNNB1-1507-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54542-img0006.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/1507) - Azide and BSA Free [NBP2-54542] - beta-Catenin Antibody (CTNNB1/1507) [NBP2-54542] - Formalin-fixed, paraffin-embedded Rat Colon stained with Beta-Catenin Monoclonal Antibody (CTNNB1/1507).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CTNNB1-1507-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54542-img0007.jpg)
![Immunohistochemistry-Paraffin: Lipocalin-2/NGAL Antibody (972737) [MAB17572] - Antibody was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue.](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-972737-Immunohistochemistry-Paraffin-MAB17572-img0002.jpg "Immunohistochemistry-Paraffin: Lipocalin-2/NGAL Antibody (972737) [MAB17572] - Antibody was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue.")