| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76737-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95104-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52453-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90116-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26258-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45338 | |||
| Azide and BSA Free | NBP2-34805-0.1mg | |||
![Western Blot: SUMO1 Antibody [NBP1-76737] - HL-60 cell lysate with sumo antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL.](http://images.novusbio.com/fullsize/SUMO1-Antibody-Western-Blot-NBP1-76737-img0001.jpg "Western Blot: SUMO1 Antibody [NBP1-76737] - HL-60 cell lysate with sumo antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL.")
![Immunocytochemistry/ Immunofluorescence: SUMO1 Antibody - BSA Free [NBP1-76737] - Immunofluorescence of SUMO1 in HL60 cells with SUMO1 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76737_rabbit-polyclonal-sumo1-antibody-271120242013215.jpg)
![Immunocytochemistry/ Immunofluorescence: SUMO1 Antibody - BSA Free [NBP1-76737] - Immunocytochemistry of SUMO1 in HL60 cells with SUMO1 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76737_rabbit-polyclonal-sumo1-antibody-2711202420352482.jpg)
![Western Blot: NAPA Antibody [NBP2-95104] - Paraffin-embedded rat brain using NAPA .](http://images.novusbio.com/fullsize/NAPA-Antibody-Western-Blot-NBP2-95104-img0003.jpg "Western Blot: NAPA Antibody [NBP2-95104] - Paraffin-embedded rat brain using NAPA .")
![Immunohistochemistry-Paraffin: NAPA Antibody [NBP2-95104] - Paraffin-embedded mouse brain using NAPA .](http://images.novusbio.com/fullsize/NAPA-Antibody-Immunohistochemistry-Paraffin-NBP2-95104-img0002.jpg)
![Western Blot: NAPA Antibody [NBP2-95104] - Analysis of extracts of various cell lines, using NAPA .Exposure time: 90s.](http://images.novusbio.com/fullsize/NAPA-Antibody-Western-Blot-NBP2-95104-img0004.jpg)
![Immunohistochemistry-Paraffin: NAPA Antibody [NBP2-95104] - Paraffin-embedded human prostate using NAPA .](http://images.novusbio.com/fullsize/NAPA-Antibody-Immunohistochemistry-Paraffin-NBP2-95104-img0001.jpg)
![Western Blot: USP21 Antibody - Azide and BSA Free [USP21] - Western blot analysis of lysates from Mouse brain, using USP21 Rabbit pAb at 1:700 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-03947_rabbit-polyclonal-usp21-antibody-5220251638021.jpg "Western Blot: USP21 Antibody - Azide and BSA Free [USP21] - Western blot analysis of lysates from Mouse brain, using USP21 Rabbit pAb at 1:700 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Immunocytochemistry/ Immunofluorescence: USP21 Antibody - Azide and BSA Free [USP21] - Immunofluorescence analysis of PC-12 cells using USP21 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03947_rabbit-polyclonal-usp21-antibody-52202516293926.jpg)
![Immunocytochemistry/ Immunofluorescence: USP21 Antibody - Azide and BSA Free [USP21] - Immunofluorescence analysis of HeLa cells using USP21 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03947_rabbit-polyclonal-usp21-antibody-522025162802.jpg)
![Immunocytochemistry/ Immunofluorescence: USP21 Antibody - Azide and BSA Free [USP21] - Immunofluorescence analysis of RAW264.7 cells using USP21 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03947_rabbit-polyclonal-usp21-antibody-5220251628058.jpg)
![Western Blot: Gemin 3 Antibody (7B2B1) [NBP2-52453] - Analysis using DDX20 mAb against human DDX20 (AA: 725-824) recombinant protein. (Expected MW is 38.4 kDa)](http://images.novusbio.com/fullsize/Gemin-3-Antibody-7B2B1-Western-Blot-NBP2-52453-img0003.jpg "Western Blot: Gemin 3 Antibody (7B2B1) [NBP2-52453] - Analysis using DDX20 mAb against human DDX20 (AA: 725-824) recombinant protein. (Expected MW is 38.4 kDa)")
![Immunocytochemistry/Immunofluorescence: Gemin 3 Antibody (7B2B1) [NBP2-52453] - Analysis of HeLa cells using DDX20 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/Gemin-3-Antibody-7B2B1-Immunofluorescence-NBP2-52453-img0002.jpg)
![Western Blot: Gemin 3 Antibody (7B2B1) [NBP2-52453] - Analysis using DDX20 mAb against HEK293 (1) and DDX20 (AA: 725-824)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Gemin-3-Antibody-7B2B1-Western-Blot-NBP2-52453-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Gemin 3 Antibody (7B2B1) [NBP2-52453] - Analysis of HeLa cells using DDX20 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/Gemin-3-Antibody-7B2B1-Immunofluorescence-NBP2-52453-img0001.jpg)
![ELISA: Gemin 3 Antibody (7B2B1) [NBP2-52453] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/Gemin-3-Antibody-7B2B1-ELISA-NBP2-52453-img0005.jpg)
![Western Blot: Vimentin Antibody (A6-C1-R) [NBP3-33092] - Western blot analysis of Vimentin on different lysates with Mouse anti-Vimentin antibody (NBP3-33092) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: C2C12 cell lysate Lane 3: L6 cell lysate Lane 4: Daudi cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33092) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33092_mouse-vimentin-mab-a6-c1-r-288202414191534.jpg "Western Blot: Vimentin Antibody (A6-C1-R) [NBP3-33092] - Western blot analysis of Vimentin on different lysates with Mouse anti-Vimentin antibody (NBP3-33092) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: C2C12 cell lysate Lane 3: L6 cell lysate Lane 4: Daudi cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33092) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Vimentin Antibody (A6-C1-R) [NBP3-33092] - Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-Vimentin antibody (NBP3-33092) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33092) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33092_mouse-vimentin-mab-a6-c1-r-298202414304186.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody (A6-C1-R) [NBP3-33092] - Immunocytochemistry analysis of HeLa cells labeling Vimentin with Mouse anti-Vimentin antibody (NBP3-33092) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Vimentin antibody (NBP3-33092) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33092_mouse-vimentin-mab-a6-c1-r-2920241192398.jpg)
![Western Blot: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33072) at 1/500 dilution. Lane 1: HepG2 cell lysate Lane 2: Hela cell lysate Lane 3: 293T cell lysate Lane 4: K-562 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse brain tissue lysate Lane 9: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33072) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33072_mouse-uba3-mab-1b5-2-3-r-28820241419150.jpg "Western Blot: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33072) at 1/500 dilution. Lane 1: HepG2 cell lysate Lane 2: Hela cell lysate Lane 3: 293T cell lysate Lane 4: K-562 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse brain tissue lysate Lane 9: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33072) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-UBA3 antibody (NBP3-33072) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33072) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33072_mouse-uba3-mab-1b5-2-3-r-29820241420944.jpg)
![Immunocytochemistry/ Immunofluorescence: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Immunocytochemistry analysis of SiHa cells labeling UBA3 with Mouse anti-UBA3 antibody (NBP3-33072) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-UBA3 antibody (NBP3-33072) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33072_mouse-uba3-mab-1b5-2-3-r-29202410334093.jpg)
![Western Blot: CHIP/STUB1 Antibody (034) [NBP2-90116] - Anti-CHIP/STUB1 rabbit monoclonal antibody at 1:500 dilution. Lane A: HELA Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lane C: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-034-Western-Blot-NBP2-90116-img0005.jpg "Western Blot: CHIP/STUB1 Antibody (034) [NBP2-90116] - Anti-CHIP/STUB1 rabbit monoclonal antibody at 1:500 dilution. Lane A: HELA Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lane C: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: CHIP/STUB1 Antibody (034) [NBP2-90116] - Flow cytometric analysis of Human STUB1 expression on Hela cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human STUB1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-034-Flow-Cytometry-NBP2-90116-img0004.jpg)
![Immunoprecipitation: CHIP/STUB1 Antibody (034) [NBP2-90116] - STUB1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg 293T Whole Cell LysateLane C:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-STUB1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-STUB1 rabbit monoclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size: 35 kDa](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-034-Immunoprecipitation-NBP2-90116-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CHIP/STUB1 Antibody (034) [NBP2-90116] - Immunofluorescence staining of STUB1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-90116 antibody (dilution ratio 1:300) overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Nucleus.](http://images.novusbio.com/fullsize/nbp2-90116_rabbit-monoclonal-chip-stub1-antibody-034-235202514331566.png)
![Western Blot: ATG12 Antibody (9411) [NBP2-43753] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 12 % SDS-PAGE ATG12 antibody [9411] dilution: 1:1000](http://images.novusbio.com/fullsize/ATG12-Antibody-9411-Western-Blot-NBP2-43753-img0001.jpg "Western Blot: ATG12 Antibody (9411) [NBP2-43753] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 12 % SDS-PAGE ATG12 antibody [9411] dilution: 1:1000")
![Western Blot: ATG12 Antibody (9411) [NBP2-43753] - Analysis of A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug C8D30 whole cell lysate/extract D. 30 ug NIH-3T3 whole cell lysate/extract E. 30 ug BCL-1 whole cell lysate/extract F. 30 ug Raw264.7 whole cell lysate/extract G. 30 ug C2C12 whole cell lysate/extract 12 % SDS-PAGE ATG12 antibody [9411] dilution: 1:1000.](http://images.novusbio.com/fullsize/ATG12-Antibody-9411-Western-Blot-NBP2-43753-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG12 antibody [GT9411] (NBP2-43753) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43753_mouse-monoclonal-atg12-antibody-9411-western-blot-261120251943817.jpg)
![Western Blot: RBX1 Antibody [NBP2-20113] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 15% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/RBX1-Antibody-Western-Blot-NBP2-20113-img0001.jpg "Western Blot: RBX1 Antibody [NBP2-20113] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 15% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: RBX1 Antibody [NBP2-20113] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with RBX1 antibody (NBP2-20113) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20113_rabbit-polyclonal-rbx1-antibody-910202420104483.jpg)
![Immunohistochemistry: USP7 Antibody (7F10) [NBP3-26258] - Image of USP7 Antibody (7F10) diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26258_rabbit-usp7-mab-7f10-262202415253324.jpg "Immunohistochemistry: USP7 Antibody (7F10) [NBP3-26258] - Image of USP7 Antibody (7F10) diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: USP7 Antibody (7F10) [NBP3-26258] - Image of USP7 Antibody (7F10) diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26258_rabbit-usp7-mab-7f10-2722024827493.jpg)
![Western Blot: USP7 Antibody (7F10) [NBP3-26258] - Positive Western Blot detected in: K562 whole cell lysate. All lanes: USP7 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 129, 127 kDa Observed band size: 140 kDa](http://images.novusbio.com/fullsize/nbp3-26258_rabbit-usp7-mab-7f10-282202411363024.jpg)
![Immunocytochemistry/Immunofluorescence: USP7 Antibody (7F10) [NBP3-26258] - Staining of Hela Cells with USP7 Antibody (7F10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26258_rabbit-usp7-mab-7f10-28220241312224.jpg)
![Immunohistochemistry-Paraffin: SUMO1 Antibody (SPM571) [NBP2-32902] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with SUMO1 Antibody (SPM571)](http://images.novusbio.com/fullsize/SUMO1-Antibody-SPM571-Immunohistochemistry-Paraffin-NBP2-32902-img0005.jpg "Immunohistochemistry-Paraffin: SUMO1 Antibody (SPM571) [NBP2-32902] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with SUMO1 Antibody (SPM571)")
![Western Blot: LRH-1/NR5A2 Antibody [NBP1-32489] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NR5A2 antibody [N2C3] diluted at 1:500.](http://images.novusbio.com/fullsize/LRH-1-NR5A2-Antibody-Western-Blot-NBP1-32489-img0007.jpg "Western Blot: LRH-1/NR5A2 Antibody [NBP1-32489] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NR5A2 antibody [N2C3] diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: LRH-1/NR5A2 Antibody [NBP1-32489] - Paraformaldehyde-fixed Human ESC, using NR5A2 antibody at 1:400 dilution.](http://images.novusbio.com/fullsize/LRH-1-NR5A2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32489-img0004.jpg)