| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52665-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62554-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88961-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26247-50ul | |||
![Immunocytochemistry/ Immunofluorescence: Slit2 Antibody (JE31-87) [NBP3-32955] - Immunocytochemistry analysis of A549 cells labeling Slit2 with Rabbit anti-Slit2 antibody (NBP3-32955) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Slit2 antibody (NBP3-32955) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32955_rabbit-slit2-mab-je31-87-29202410484851.jpg "Immunocytochemistry/ Immunofluorescence: Slit2 Antibody (JE31-87) [NBP3-32955] - Immunocytochemistry analysis of A549 cells labeling Slit2 with Rabbit anti-Slit2 antibody (NBP3-32955) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Slit2 antibody (NBP3-32955) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: Slit2 Antibody (JE31-87) [NBP3-32955] - Flow cytometric analysis of A549 cells labeling Slit2.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32955, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32955_rabbit-slit2-mab-je31-87-29202416372174.jpg)
![Western blot analysis on different lysates with NBP3-32955 at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (15 µg/Lane) Lane 2: Human brain tissue lysate (30 µg/Lane) Lane 3: Mouse brain tissue lysate (30 µg/Lane) Lane 4: Rat brain tissue lysate (30 µg/Lane) Predicted band size: 170 kDa Observed band size: 50/200 kDa Exposure time: 28 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32955 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32955_-western-blot-639204597796446487.jpg)
![Immunohistochemistry-Paraffin: Complement C3d Antibody (E28-P) [NBP1-79074] - Formalin-fixed and paraffin-embedded human skin biopsy (4 um) from lesion of the early pemphigus vulgaris (without blister formation), stained with anti-C3d complement antibody shows strong positive intraepidermal intercellular immunostaining.](http://images.novusbio.com/fullsize/Complement-C3d-Antibody-E28-P-Immunohistochemistry-Paraffin-NBP1-79074-img0002.jpg "Immunohistochemistry-Paraffin: Complement C3d Antibody (E28-P) [NBP1-79074] - Formalin-fixed and paraffin-embedded human skin biopsy (4 um) from lesion of the early pemphigus vulgaris (without blister formation), stained with anti-C3d complement antibody shows strong positive intraepidermal intercellular immunostaining.")
![Flow Cytometry: Integrin beta 1/CD29 Antibody (K20) - Chimeric [NBP2-52665] - Flow-cytometry using anti-CD29 antibody K20. Human leukocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of K20 (NBP2-52665, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-K20-Chimeric-Flow-Cytometry-NBP2-52665-img0001.jpg "Flow Cytometry: Integrin beta 1/CD29 Antibody (K20) - Chimeric [NBP2-52665] - Flow-cytometry using anti-CD29 antibody K20. Human leukocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of K20 (NBP2-52665, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![In vitro assay: Recombinant Human SOD1/Cu-Zn SOD Pre-formed Fibrils Protein [NBP3-14783] - Thioflavin T assay of SOD1 pre-formed fibrils (PFFs) (NBP3-14783) combined with SOD1 monomers . Pre-formed fibrils seed the aggregation of monomers, leading to an increase in Thioflavin T fluorescence](http://images.novusbio.com/fullsize/Recombinant-Human-SOD1-Cu-Zn-SOD-Pre-formed-Fibrils-Protein-In-vitro-assay-NBP3-14783-img0001.jpg "In vitro assay: Recombinant Human SOD1/Cu-Zn SOD Pre-formed Fibrils Protein [NBP3-14783] - Thioflavin T assay of SOD1 pre-formed fibrils (PFFs) (NBP3-14783) combined with SOD1 monomers . Pre-formed fibrils seed the aggregation of monomers, leading to an increase in Thioflavin T fluorescence")
![Electron Microscopy: Recombinant Human SOD1/Cu-Zn SOD Pre-formed Fibrils Protein [NBP3-14783] - TEM of human SOD1 pre-formed fibrils (PFFs)](http://images.novusbio.com/fullsize/Recombinant Human SOD1-Cu-Zn SOD Pre-formed Fibrils Protein-Electron Microscopy-NBP3-14783-img0003.jpg)
![Electron Microscopy: Recombinant Human SOD1/Cu-Zn SOD Pre-formed Fibrils Protein [NBP3-14783] - TEM of human SOD1 pre-formed fibrils (PFFs)](http://images.novusbio.com/fullsize/Recombinant-Human-SOD1-Cu-Zn-SOD-Pre-formed-Fibrils-Protein-Electron-Microscopy-NBP3-14783-img0002.jpg)
![Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Western-Blot-NBP2-62554-img0003.jpg "Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.")
![Immunohistochemistry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Immunohistochemical staining of human placenta tissue using anti-CD34 antibody. My10 Anti-CD34 staining of paraffin embedded human placenta tissue using the rabbit-chimeric version of My10 (NBP2-62554). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62554 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Strong endothelial staining of within villi may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Immunohistochemistry-NBP2-62554-img0002.jpg)
![Flow Cytometry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Flow-cytometry using anti-CD34 antibody My10. Human leukocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of My10 (NBP2-62554, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Flow-Cytometry-NBP2-62554-img0001.jpg)
![Western Blot: PPP2R5C Antibody [NBP1-88961] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/PPP2R5C-Antibody-Western-Blot-NBP1-88961-img0005.jpg "Western Blot: PPP2R5C Antibody [NBP1-88961] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunohistochemistry-Paraffin: PPP2R5C Antibody [NBP1-88961] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize/PPP2R5C-Antibody-Immunohistochemistry-Paraffin-NBP1-88961-img0002.jpg)
![Western Blot: VAMP-1 Antibody [NBP2-15159] - Sample (30 ug of whole cell lysate) A: NT2D1 15% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/VAMP-1-Antibody-Western-Blot-NBP2-15159-img0005.jpg "Western Blot: VAMP-1 Antibody [NBP2-15159] - Sample (30 ug of whole cell lysate) A: NT2D1 15% SDS PAGE, antibody diluted at 1:500.")
![Immunohistochemistry-Paraffin: VAMP-1 Antibody [NBP2-15159] - VAMP-1 antibody detects VAMP-1 protein on mouse fore at Dilution: 1:500.](http://images.novusbio.com/fullsize/VAMP-1-Antibody-Immunohistochemistry-Paraffin-NBP2-15159-img0008.jpg)
![Immunohistochemistry-Paraffin: VAMP-1 Antibody [NBP2-15159] - Paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/VAMP-1-Antibody-Immunohistochemistry-Paraffin-NBP2-15159-img0004.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody (6F6) [NBP3-26247] - Positive Western Blot detected in: U87 whole cell lysate, MCF-7 whole cell lysate, PC3 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate, THP-1 whole cell lysate, A549 whole cell lysate, Mouse brain tissue, Rat brain tissue. All lanes: 14-3-3 beta/alpha Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 29, 28 kDa Observed band size: 25-35 kDa](http://images.novusbio.com/fullsize/nbp3-26247_rabbit-14-3-3-beta-alpha-mab-6f6-282202411351616.jpg "Western Blot: 14-3-3 beta/alpha Antibody (6F6) [NBP3-26247] - Positive Western Blot detected in: U87 whole cell lysate, MCF-7 whole cell lysate, PC3 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate, THP-1 whole cell lysate, A549 whole cell lysate, Mouse brain tissue, Rat brain tissue. All lanes: 14-3-3 beta/alpha Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 29, 28 kDa Observed band size: 25-35 kDa")
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody (6F6) [NBP3-26247] - Staining of PC-3 cell with 14-3-3 beta/alpha Antibody (6F6) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 494-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26247_rabbit-14-3-3-beta-alpha-mab-6f6-282202412493639.jpg)
![Flow Cytometry: 14-3-3 beta/alpha Antibody (6F6) [NBP3-26247] - Overlay Peak curve showing Hela cells stained with 14-3-3 beta/alpha Antibody (6F6) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26247_rabbit-14-3-3-beta-alpha-mab-6f6-282202414183655.jpg)
![Immunohistochemistry-Paraffin: PRAS40 Antibody [NBP2-19924] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PRAS40-Antibody-Immunohistochemistry-Paraffin-NBP2-19924-img0003.jpg "Immunohistochemistry-Paraffin: PRAS40 Antibody [NBP2-19924] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.")
![Western Blot: GABA-AR alpha 1 Antibody [NBP2-16565] - GABA A Receptor alpha 1 antibody detects GABA A Receptor alpha 1 protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GABA A Receptor alpha 1 antibody diluted by 1:500.](http://images.novusbio.com/fullsize/GABA-AR-alpha-1-Antibody-Western-Blot-NBP2-16565-img0005.jpg "Western Blot: GABA-AR alpha 1 Antibody [NBP2-16565] - GABA A Receptor alpha 1 antibody detects GABA A Receptor alpha 1 protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GABA A Receptor alpha 1 antibody diluted by 1:500.")
![Immunohistochemistry-Paraffin: GABA-A R alpha 1 Antibody [NBP2-16565] - Paraffin-embedded rat brain. GABA A Receptor alpha 1 antibody [N1C1] diluted at 1:500.](http://images.novusbio.com/fullsize/GABA-A-R-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-16565-img0003.jpg)
![Immunohistochemistry-Paraffin: GABA-A R alpha 1 Antibody [NBP2-16565] - Paraffin-embedded rat brain. GABA A Receptor alpha 1 antibody [N1C1] diluted at 1:500.](http://images.novusbio.com/fullsize/GABA-A-R-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-16565-img0004.jpg)