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![Western Blot: APP Antibody (J4H9) [NBP1-04263] - The lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human APP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Mouse brain tissue lysate.](http://images.novusbio.com/fullsize/APP-Antibody-J4H9-Western-Blot-NBP1-04263-img0003.jpg "Western Blot: APP Antibody (J4H9) [NBP1-04263] - The lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human APP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Mouse brain tissue lysate.")
![Immunocytochemistry/Immunofluorescence: APP Antibody (J4H9) [NBP1-04263] - Analysis of APP/Protease Nexin II in U87MG cells. The cell was stained using a dilution of (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).](http://images.novusbio.com/fullsize/APP-Antibody-J4H9-Immunocytochemistry-Immunofluorescence-NBP1-04263-img0002.jpg)
![Flow Cytometry: APP Antibody (J4H9) [NBP1-04263] - Flow analysis of APP/Protease Nexin II in 293T cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/APP-Antibody-J4H9-Flow-Cytometry-NBP1-04263-img0001.jpg)
![Western Blot: Notch-2 Antibody [NBP1-19125] - Whole cell lysate from HeLa and 293T cells. Notch2 was also immunoprecipitated by rabbit anti-Notch2 antibody NBP1-19124.](http://images.novusbio.com/fullsize/Notch-2-Antibody-Western-Blot-NBP1-19125-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Notch-2 Antibody [NBP1-19125] - Whole cell lysate from HeLa and 293T cells. Notch2 was also immunoprecipitated by rabbit anti-Notch2 antibody NBP1-19124.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: Notch-2 Antibody [NBP1-19125] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Notch2 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Notch-2-Antibody-Immunohistochemistry-Paraffin-NBP1-19125-img0003.jpg)
![Western Blot: CaMKIV Antibody [NB120-3557] - Analysis of rat brain extract.](http://images.novusbio.com/fullsize/CaMKIV-Antibody-Western-Blot-NB120-3557-img0004.jpg "Western Blot: CaMKIV Antibody [NB120-3557] - Analysis of rat brain extract.")
![Immunohistochemistry-Paraffin: GABA-B R1 Antibody [NBP1-52389] - Analysis of anti-GABBR1 antibody with human brain, cortex at concentration 3.75 ug/ml.](http://images.novusbio.com/fullsize/GABA-B-R1-Antibody-Immunohistochemistry-Paraffin-NBP1-52389-img0002.jpg "Immunohistochemistry-Paraffin: GABA-B R1 Antibody [NBP1-52389] - Analysis of anti-GABBR1 antibody with human brain, cortex at concentration 3.75 ug/ml.")
![Immunohistochemistry-Paraffin: GABA-B R1 Antibody [NLS5332] - Anti-GABABR1 / GABBR1 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GABA-B-R1-Antibody-Immunohistochemistry-Paraffin-NLS5332-img0003.jpg "Immunohistochemistry-Paraffin: GABA-B R1 Antibody [NLS5332] - Anti-GABABR1 / GABBR1 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: GABA-B R1 Antibody [NLS5332] - GABA B Receptor 1 Antibody [NLS5332] - IHC analysis of Brain, Neurons and glia using NLS5332 at 7 ug/ ml.](http://images.novusbio.com/fullsize/GABA-B-R1-Antibody-Immunohistochemistry-Paraffin-NLS5332-img0002.jpg)
![Western Blot: VAMP3/Cellubrevin Antibody (2H8R8) [NBP3-16705] - Analysis of extracts of various cell lines, using VAMP3/VAMP3/Cellubrevin Rabbit mAb (NBP3-16705) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/VAMP3-Cellubrevin-Antibody-2H8R8-Western-Blot-NBP3-16705-img0002.jpg "Western Blot: VAMP3/Cellubrevin Antibody (2H8R8) [NBP3-16705] - Analysis of extracts of various cell lines, using VAMP3/VAMP3/Cellubrevin Rabbit mAb (NBP3-16705) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: VAMP3/Cellubrevin Antibody (2H8R8) [NBP3-16705] - Analysis of extracts of Mouse liver, using VAMP3/VAMP3/Cellubrevin Rabbit mAb (NBP3-16705) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/VAMP3-Cellubrevin-Antibody-2H8R8-Western-Blot-NBP3-16705-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: VAMP3/Cellubrevin Antibody (2H8R8) [NBP3-16705] - Immunofluorescence analysis of U-2 OS cells using VAMP3/VAMP3/Cellubrevin Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16705_rabbit-vamp3-cellubrevin-mab-2h8r8-8220252010497.jpg)
![Immunohistochemistry-Paraffin: Connexin 43/GJA1 Antibody [NB110-55546] - Analysis of anti-GJA1 / Connexin 43 antibody with human testis, seminiferous tubules and Leydig cells at concentration 20 ug/ml.](http://images.novusbio.com/fullsize/Connexin-43-GJA1-Antibody-Immunohistochemistry-Paraffin-NB110-55546-img0001.jpg "Immunohistochemistry-Paraffin: Connexin 43/GJA1 Antibody [NB110-55546] - Analysis of anti-GJA1 / Connexin 43 antibody with human testis, seminiferous tubules and Leydig cells at concentration 20 ug/ml.")
![Immunohistochemistry: SPRY2 Antibody (10M8H1) [NBP3-33538] - Immunohistochemistry analysis of paraffin-embedded Human appendix using SPRY2 Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33538_rabbit-spry2-mab-10m8h1-912202414213721.jpg "Immunohistochemistry: SPRY2 Antibody (10M8H1) [NBP3-33538] - Immunohistochemistry analysis of paraffin-embedded Human appendix using SPRY2 Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.")
![Western Blot: SPRY2 Antibody (10M8H1) [NBP3-33538] - Western blot analysis of lysates from C6 cells, using SPRY2 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-33538_rabbit-spry2-mab-10m8h1-912202415121338.jpg)
![Immunohistochemistry: SPRY2 Antibody (10M8H1) [NBP3-33538] - Immunohistochemistry analysis of paraffin-embedded Rat pancreas using SPRY2 Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33538_rabbit-spry2-mab-10m8h1-912202414201360.jpg)
![Immunohistochemistry-Paraffin: Orexin R1/HCRTR1 Antibody [NLS6641] - Analysis of anti-HCRTR1 / Orexin Receptor 1 antibody of rat brain, Purkinje neurons at 6.8 ug/ml.](http://images.novusbio.com/fullsize/Orexin-R1-HCRTR1-Antibody-Immunohistochemistry-Paraffin-NLS6641-img0002.jpg "Immunohistochemistry-Paraffin: Orexin R1/HCRTR1 Antibody [NLS6641] - Analysis of anti-HCRTR1 / Orexin Receptor 1 antibody of rat brain, Purkinje neurons at 6.8 ug/ml.")
![Immunohistochemistry-Paraffin: 5-HT6 Antibody [NLS652] - Analysis of anti-5HT6 antibody with human brain, cerebellum.](http://images.novusbio.com/fullsize/5-HT6-Antibody-Immunohistochemistry-Paraffin-NLS652-img0001.jpg "Immunohistochemistry-Paraffin: 5-HT6 Antibody [NLS652] - Analysis of anti-5HT6 antibody with human brain, cerebellum.")
![Immunohistochemistry-Paraffin: 5-HT6 Antibody - BSA Free [NLS652] - Immunohistochemistry of formalin-fixed, paraffin-embedded Human, Brain, Cerebellum tissue](http://images.novusbio.com/fullsize/nls652_rabbit-5-ht6-pab-bsa-free-1092024110317.jpg)
![Western Blot: Apolipoprotein E R2/ApoE R2 Antibody [NBP2-92026] - Analysis of extracts of various cell lines, using Apolipoprotein E R2/ApoE R2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/Apolipoprotein-E-R2-ApoE-R2-Antibody-Western-Blot-NBP2-92026-img0001.jpg "Western Blot: Apolipoprotein E R2/ApoE R2 Antibody [NBP2-92026] - Analysis of extracts of various cell lines, using Apolipoprotein E R2/ApoE R2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.")
![Immunohistochemistry: Apolipoprotein E R2/ApoE R2 Antibody [NBP2-92026] - Immunofluorescence analysis of Human placenta using ApoER2/Apolipoprotein E R2/ApoE R2 antibody (NBP2-92026) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Apolipoprotein-E-R2-ApoE-R2-Antibody-Immunohistochemistry-NBP2-92026-img0002.jpg)
![Western Blot: Profilin 1 Antibody [NBP2-99538] - Anti-Profilin 1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse spleen tissue lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 15 kDa. Observed band size: 15 kDa](http://images.novusbio.com/fullsize/Profilin-1-Antibody-Western-Blot-NBP2-99538-img0005.jpg "Western Blot: Profilin 1 Antibody [NBP2-99538] - Anti-Profilin 1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse spleen tissue lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 15 kDa. Observed band size: 15 kDa")
![Immunohistochemistry-Paraffin: Profilin 1 Antibody [NBP2-99538] - Immunochemical staining Profilin 1 in rat kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-99538-img0004.jpg)
![Immunohistochemistry-Paraffin: Profilin 1 Antibody [NBP2-99538] - Immunochemical staining Profilin 1 in mouse colon with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-99538-img0002.jpg)
![Immunohistochemistry-Paraffin: Profilin 1 Antibody [NBP2-99538] - Immunochemical staining Profilin 1 in mouse kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-99538-img0003.jpg)