| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18543-100ul | |||
| BSA Free | NBP3-18543-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21163-100ul | |||
| BSA Free | NBP3-21163-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05535-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86988-25ul | |||
![Western Blot: DARPP-32 [p Thr34] Antibody [NB300-224]](http://images.novusbio.com/fullsize/DARPP-32-[p-Thr34]-Antibody-Western-Blot-NB300-224-img0001.jpg "Western Blot: DARPP-32 [p Thr34] Antibody [NB300-224]")
![Western Blot: Synaptotagmin 1 [p Ser309] Antibody [NB300-236] - Western blot of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k synaptotagmin phosphorylated at Ser309 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Ser309 synaptotagmin antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.](http://images.novusbio.com/fullsize/Synaptotagmin-1-[p-Ser309]-Antibody-Western-Blot-NB300-236-img0001.jpg "Western Blot: Synaptotagmin 1 [p Ser309] Antibody [NB300-236] - Western blot of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k synaptotagmin phosphorylated at Ser309 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Ser309 synaptotagmin antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.")
![Immunohistochemistry: Synaptotagmin-1 [p Ser309] Antibody [NB300-236] - Immunostaining of 14 DIV rat cortical neurons showing synaptotagmin when phosphorylated at Ser309 in red and PSD95 in green. Photo courtesy of Gang Liu.](http://images.novusbio.com/fullsize/Synaptotagmin-1-[p-Ser309]-Antibody-Immunohistochemistry-NB300-236-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody [NB100-41105] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/NMDAR1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-41105-img0002.jpg "Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody [NB100-41105] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody [NB100-41105] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/NMDAR1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-41105-img0001.jpg)
![Western Blot: CSN1 Antibody [NB100-1237] - Human Testis lysate (RIPA buffer, 30 ug total protein). Antibody at 0.5 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CSN1-Antibody-Western-Blot-NB100-1237-img0002.jpg "Western Blot: CSN1 Antibody [NB100-1237] - Human Testis lysate (RIPA buffer, 30 ug total protein). Antibody at 0.5 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: CSN1 Antibody [NB100-1237] - Staining of paraffin embedded Human Tonsil. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/CSN1-Antibody-Immunohistochemistry-Paraffin-NB100-1237-img0005.jpg)
![Immunohistochemistry-Paraffin: CSN1 Antibody [NB100-1237] - Staining of paraffin embedded Human Prostate. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/CSN1-Antibody-Immunohistochemistry-Paraffin-NB100-1237-img0003.jpg)
![Immunohistochemistry-Paraffin: CSN1 Antibody [NB100-1237] - Staining of paraffin embedded Human Testis. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/CSN1-Antibody-Immunohistochemistry-Paraffin-NB100-1237-img0004.jpg)
![Western Blot: PHOX2A Antibody (4F6) [H00000401-M01] - Analysis of PHOX2A expression in transfected 293T cell line by PHOX2A monoclonal antibody (M01), clone 4F6.Lane 1: PHOX2A transfected lysate(29.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PHOX2A-Antibody-4F6-Western-Blot-H00000401-M01-img0010.jpg "Western Blot: PHOX2A Antibody (4F6) [H00000401-M01] - Analysis of PHOX2A expression in transfected 293T cell line by PHOX2A monoclonal antibody (M01), clone 4F6.Lane 1: PHOX2A transfected lysate(29.7 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PHOX2A Antibody (4F6) [H00000401-M01] - Analysis of monoclonal antibody to PHOX2A on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PHOX2A-Antibody-4F6-Immunocytochemistry-Immunofluorescence-H00000401-M01-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PHOX2A Antibody (4F6) [H00000401-M01] - Analysis of PHOX2A over-expressed 293 cell line, cotransfected with PHOX2A Validated Chimera RNAi ( Cat # H00000401-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PHOX2A monoclonal antibody (M01), clone 4F6 (Cat # H00000401-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/PHOX2A-Antibody-4F6-Western-Blot-H00000401-M01-img0008.jpg)
![Western Blot: PHOX2A Antibody (4F6) [H00000401-M01] - PHOX2A monoclonal antibody (M01), clone 4F6 Analysis of PHOX2A expression in PC-12.](http://images.novusbio.com/fullsize/PHOX2A-Antibody-4F6-Western-Blot-H00000401-M01-img0009.jpg)
![Western Blot: Von Hippel Lindau Antibody [H00007428-D01P] - Analysis of VHL expression in mouse lung.](http://images.novusbio.com/fullsize/Von-Hippel-Lindau-Antibody-Western-Blot-H00007428-D01P-img0004.jpg "Western Blot: Von Hippel Lindau Antibody [H00007428-D01P] - Analysis of VHL expression in mouse lung.")
![Western Blot: Von Hippel Lindau Antibody [H00007428-D01P] - Analysis of VHL expression in transfected 293T cell line by VHL polyclonal antibody.Lane 1: VHL transfected lysate(19.70 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Von-Hippel-Lindau-Antibody-Western-Blot-H00007428-D01P-img0005.jpg)
![Western Blot: xCT Antibody (A7C6-R) [NBP3-33104] - Western blot analysis of xCT on different lysates with Mouse anti-xCT antibody (NBP3-33104) at 1/500 dilution. Lane 1: HT-29 cell lysate Lane 2: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33104) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-288202414191525.jpg "Western Blot: xCT Antibody (A7C6-R) [NBP3-33104] - Western blot analysis of xCT on different lysates with Mouse anti-xCT antibody (NBP3-33104) at 1/500 dilution. Lane 1: HT-29 cell lysate Lane 2: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33104) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: xCT Antibody (A7C6-R) [NBP3-33104] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-xCT antibody (NBP3-33104) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33104) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-298202413585586.jpg)
![Immunocytochemistry/ Immunofluorescence: xCT Antibody (A7C6-R) [NBP3-33104] - Immunocytochemistry analysis of HT-29 cells labeling xCT with Mouse anti-xCT antibody (NBP3-33104) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-xCT antibody (NBP3-33104) at 1/200 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-2920241114813.jpg)
![Flow Cytometry: xCT Antibody (A7C6-R) [NBP3-33104] - Flow cytometric analysis of A549 cells labeling xCT. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-33104, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-29202416372152.jpg)
![Immunohistochemistry: ABCG2/CD338 Antibody (JF0994) [NBP3-31988] - Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-ABCG2/CD338 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-31988, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-31988_rabbit-abcg2-cd338-mab-jf0994-298202413585550.jpg "Immunohistochemistry: ABCG2/CD338 Antibody (JF0994) [NBP3-31988] - Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-ABCG2/CD338 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-31988, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: ABCG2/CD338 Antibody (JF0994) [NBP3-31988] - ICC staining of ABCG2/CD338 in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP3-31988, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-31988_rabbit-abcg2-cd338-mab-jf0994-2920241033403.jpg)
![Flow Cytometry: ABCG2/CD338 Antibody (JF0994) [NBP3-31988] - Flow cytometric analysis of ABCG2/CD338 was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody (NBP3-31988, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-31988_rabbit-abcg2-cd338-mab-jf0994-29202416372166.jpg)
![Western Blot: ABCG2/CD338 Antibody (JF0994) [NBP3-31988] - Western blot analysis of ABCG2/CD338 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (NBP3-31988, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: human placenta tissue lysate Lane 3: Hela cell lysate Lane 4: 293T cell lysate](http://images.novusbio.com/fullsize/nbp3-31988_rabbit-abcg2-cd338-mab-jf0994-3920248453510.jpg)
![Western Blot: GAP-43 Antibody [NBP3-05535] - Western blot analysis of different tissue and cell lysates using GAP-43 Antibody at a dilution of 1:5,000, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] C6 cells, [7] SH-SY5Y cells. The single band at the 43kDa mark corresponds to the GAP-43 protein. The protein is expressed in rodent and human neurons and neuronal derived cells but not in C6 cells which are of glial origin.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Western-Blot-NBP3-05535-img0002.jpg "Western Blot: GAP-43 Antibody [NBP3-05535] - Western blot analysis of different tissue and cell lysates using GAP-43 Antibody at a dilution of 1:5,000, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] C6 cells, [7] SH-SY5Y cells. The single band at the 43kDa mark corresponds to the GAP-43 protein. The protein is expressed in rodent and human neurons and neuronal derived cells but not in C6 cells which are of glial origin.")
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NBP3-05535] - Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with GAP-43 Antibody at a dilution of 1:1,000, in red, and costained with chicken pAb to MAP2 at a dilution of 1:10,000, in green. The blue is DAPI staining of nuclear DNA. GAP-43 antibody labels protein expressed in the axonal membrane of the neuronal cells, while the MAP2 antibody stains dendrites and perikarya of neurons.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05535-img0001.jpg)
