| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-60497-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03303-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98832-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97603-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38103-20ul | |||
![In vitro assay: Recombinant Human gamma-Synuclein Active, Monomer Protein [NBP3-14778] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in gamma synuclein fibrils. Increased fluorescence (correlated to gamma synuclein fibrillization) is seen when human gamma synuclein monomer (NBP3-14778) is combined with human gamma synuclein PFFs .](http://images.novusbio.com/fullsize/Recombinant-Human-gamma-Synuclein-Active--Monomer-Protein-In-vitro-assay-NBP3-14778-img0001.jpg "In vitro assay: Recombinant Human gamma-Synuclein Active, Monomer Protein [NBP3-14778] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in gamma synuclein fibrils. Increased fluorescence (correlated to gamma synuclein fibrillization) is seen when human gamma synuclein monomer (NBP3-14778) is combined with human gamma synuclein PFFs .")
![SDS-Page: Recombinant Human gamma-Synuclein Active, Monomer Protein [NBP3-14778] - Coomassie gel stain of Human gamma-Synuclein Monomer (NBP3-14778). Load: 2 ug.](http://images.novusbio.com/fullsize/Recombinant Human gamma-Synuclein Active- Monomer Protein-SDS-Page-NBP3-14778-img0002.jpg)
![Immunohistochemistry;NPM1 Antibody[NBP3-29483]Detection of human and mouse NPM1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma and mouse renal cell carcinoma . Antibody: Affinity purified rabbit anti-NPM1 used at a dilution of 1:1,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-29484_rabbit-npm1-pab-78202410153513.jpg "Immunohistochemistry;NPM1 Antibody[NBP3-29483]Detection of human and mouse NPM1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma and mouse renal cell carcinoma . Antibody: Affinity purified rabbit anti-NPM1 used at a dilution of 1:1,000 . Detection: DAB")
![Immunoprecipitation:NPM1 AntibodyNBP3-29484] -Detection of human NPM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NPM1 antibody NBP3-29484 used for IP at 10 µg/mg lysate. NPM1 was also immunoprecipitated by rabbit anti-NPM1 antibodies which recognize upstream epitopes. For blotting immunoprecipitated NPM1, was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/nbp3-29484_rabbit-npm1-pab-301220241941541.jpg)
![Immunohistochemistry:[NBP3-29335]-Detection of human NPM1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-NPM1.](http://images.novusbio.com/fullsize/nbp3-29335_rabbit-npm1-pab-23120251252823.jpg "Immunohistochemistry:[NBP3-29335]-Detection of human NPM1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-NPM1.")
![Immunohistochemistry:[NBP3-29334]-Detection of human EB1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-EB1.](http://images.novusbio.com/fullsize/nbp3-29334_rabbit-mapre1-pab-231202511595075.jpg "Immunohistochemistry:[NBP3-29334]-Detection of human EB1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-EB1.")
![Western Blot:[NBP3-28970]-Detection of human and mouse CD1D by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat and from mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CD1D antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-28970_rabbit-cd1d-pab-231202511585525.jpg "Western Blot:[NBP3-28970]-Detection of human and mouse CD1D by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat and from mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CD1D antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot:[NBP3-28970]-CD1d Antibody](http://images.novusbio.com/fullsize/nbp3-28970_rabbit-cd1d-pab-31120250364041.jpg)
![Western Blot: gamma-Synuclein Antibody (1H10D2) [NB110-60497] - Analysis using mouse mAb against truncated SNCG recombinant protein.](http://images.novusbio.com/fullsize/Synuclein-gamma-Antibody-1H10D2-Western-Blot-NB110-60497-img0002.jpg "Western Blot: gamma-Synuclein Antibody (1H10D2) [NB110-60497] - Analysis using mouse mAb against truncated SNCG recombinant protein.")
![Immunohistochemistry-Paraffin: gamma-Synuclein Antibody (1H10D2) [NB110-60497] - Analysis of human ovary carcinoma (left) and breast carcinoma (right), showing cytoplasmic(ovary carcinoma) localization, cytoplasmic and nuclear (breast carcinoma) localization using SNCG mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Synuclein-gamma-Antibody-1H10D2-Immunohistochemistry-Paraffin-NB110-60497-img0004.jpg)
![Western Blot: UbcH10/UBE2C Antibody [NBP3-03303] - Analysis of extracts from normal (control) and UBE2C knockout (KO) HeLa cells, using UbcH10/UBE2C antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-Knockout-Validated-NBP3-03303-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: UbcH10/UBE2C Antibody [NBP3-03303] - Analysis of extracts from normal (control) and UBE2C knockout (KO) HeLa cells, using UbcH10/UBE2C antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Western Blot: UbcH10/UBE2C Antibody (5G5L9) [NBP3-16588] - Western blot analysis of extracts of various cell lines, using UbcH10/UBE2C Rabbit mAb (NBP3-16588) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-5G5L9-Western-Blot-NBP3-16588-img0002.jpg "Western Blot: UbcH10/UBE2C Antibody (5G5L9) [NBP3-16588] - Western blot analysis of extracts of various cell lines, using UbcH10/UBE2C Rabbit mAb (NBP3-16588) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: UbcH10/UBE2C Antibody (5G5L9) [NBP3-16588] - Immunofluorescence analysis of U-2 OS cells using UbcH10/UBE2C Rabbit mAb (NBP3-16588) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-5G5L9-Immunocytochemistry-Immunofluorescence-NBP3-16588-img0001.jpg)
![Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Aurora-A-[p-Thr288]-Antibody-Western-Blot-NBP3-05434-img0001.jpg "Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.")
![Simple Western: Aurora A [p Thr288] Antibody - BSA Free [NBP3-05434] - Analysis of protein expression post-drug treatment. (A) CHLA-10 and (B) TC-71 cells treated with drugs were assessed for changes in protein expression 24 h post-treatment via capillary electrophoresis-based Wes analysis. Increased protein levels of KIF11, p-KIF11Thr926 AURKA, and p-AURKAThr288 were observed for the drug combination group, whereas KIF15 levels were noticeably lower. Similarly, enhanced cleaved-PARP expression was observed with the combination treatment. The uncropped blots are shown in Figures S8 and S9. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/37894278 ), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp3-05434_rabbit-polyclonal-aurora-a-p-thr288-antibody-308202410445511.jpg)
![Immunohistochemistry-Paraffin: TTK Antibody [NBP2-98832] - Staining of human TTK in human lung cancer with rabbit polyclonal antibody (1:1000).](http://images.novusbio.com/fullsize/TTK-Antibody-Immunohistochemistry-Paraffin-NBP2-98832-img0002.jpg "Immunohistochemistry-Paraffin: TTK Antibody [NBP2-98832] - Staining of human TTK in human lung cancer with rabbit polyclonal antibody (1:1000).")
![Immunohistochemistry-Paraffin: TTK Antibody [NBP2-98832] - Staining of human RPS27 in human lung cancer with NBP2-98832 (1:1000).](http://images.novusbio.com/fullsize/nbp2-98832_rabbit-polyclonal-ttk-antibody-235202511444413.png)
![Immunocytochemistry/Immunofluorescence: TTK Antibody [NBP2-98832] - Immunofluorescence staining of TTK in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98832 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98832_rabbit-polyclonal-ttk-antibody-23520252135167.png)
![Western Blot: NCAPH Antibody [NBP2-97603] - Anti-NCAPH rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 83 kDa](http://images.novusbio.com/fullsize/NCAPH-Antibody-Western-Blot-NBP2-97603-img0003.jpg "Western Blot: NCAPH Antibody [NBP2-97603] - Anti-NCAPH rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 83 kDa")
![Immunocytochemistry/Immunofluorescence: NCAPH Antibody [NBP2-97603] - Immunofluorescence staining of NCAPH in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97603 antibody (dilution ratio 1:500) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-97603_rabbit-polyclonal-ncaph-antibody-23520251433150.png)
![Immunocytochemistry/ Immunofluorescence: NCAPH Antibody [NBP3-38103] - Immunofluorescence analysis of U2OS cells using NCAPH Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38103_rabbit-ncaph-pab-912202415161866.jpg "Immunocytochemistry/ Immunofluorescence: NCAPH Antibody [NBP3-38103] - Immunofluorescence analysis of U2OS cells using NCAPH Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: NCAPH Antibody [NBP3-38103] - Western blot analysis of various lysates using NCAPH Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-38103_rabbit-ncaph-pab-91220241418134.jpg)