| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59431-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37290-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37294-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97249-50ul | |||
![Western Blot: CDC37 [p Ser13] Antibody (5P8B2) [NBP3-33562] - Western blot analysis of lysates from C6 cells, using CDC37 Rabbit mAb at 1:1000 dilution. C6 cells were treated by CIP(20uL/400ul) at 37C for 1 hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 3min.](http://images.novusbio.com/fullsize/nbp3-33562_rabbit-cdc37-p-ser13-mab-5p8b2-912202415152871.jpg "Western Blot: CDC37 [p Ser13] Antibody (5P8B2) [NBP3-33562] - Western blot analysis of lysates from C6 cells, using CDC37 Rabbit mAb at 1:1000 dilution. C6 cells were treated by CIP(20uL/400ul) at 37C for 1 hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 3min.")
![Western Blot: CDC37 [p Ser13] Antibody (5P8B2) [NBP3-33562] - Western blot analysis of various lysates using CDC37 Rabbit mAb at 1:1000 dilution. Both 293T cells and NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-33562_rabbit-cdc37-p-ser13-mab-5p8b2-912202415111821.jpg)
![Western Blot: ERp57/PDIA3 Antibody (9E9) [NBP2-59431] - Lane 1: HeLa cell lysates, Lane 2: HepG2 cell lysates, Lane 3: NIH3T3 cell lysates, Lane 4: Raw 264.7 cell lysates, Lane 5: LNCaP cell lysates](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-9E9-Western-Blot-NBP2-59431-img0001.jpg "Western Blot: ERp57/PDIA3 Antibody (9E9) [NBP2-59431] - Lane 1: HeLa cell lysates, Lane 2: HepG2 cell lysates, Lane 3: NIH3T3 cell lysates, Lane 4: Raw 264.7 cell lysates, Lane 5: LNCaP cell lysates")
![Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody (9E9) [NBP2-59431] - Analysis of PDIA3 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PDIA3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-9E9-Immunocytochemistry-Immunofluorescence-NBP2-59431-img0002.jpg)
![Western Blot: MDR1/ABCB1 Antibody (2U4E9) [NBP3-15660] - Western blot analysis of extracts of various cell lines, using MDR1/ABCB1 antibody (NBP3-15660) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-2U4E9-Western-Blot-NBP3-15660-img0004.jpg "Western Blot: MDR1/ABCB1 Antibody (2U4E9) [NBP3-15660] - Western blot analysis of extracts of various cell lines, using MDR1/ABCB1 antibody (NBP3-15660) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: MDR1/ABCB1 Antibody (2U4E9) [NBP3-15660] - Western blot analysis of extracts of Rat liver, using MDR1/ABCB1 antibody (NBP3-15660) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-2U4E9-Western-Blot-NBP3-15660-img0005.jpg)
![Western Blot: Dynamin Antibody [NB100-61094] - Western Blot of rat cortex lysate using NB100-61094 at 1:1000.](http://images.novusbio.com/fullsize/Dynamin-Antibody-Western-Blot-NB100-61094-img0002.jpg "Western Blot: Dynamin Antibody [NB100-61094] - Western Blot of rat cortex lysate using NB100-61094 at 1:1000.")
![Western Blot: Dynamin Antibody [NB100-61094] - Rat cortex lysate showing labeling of the ~95k Dynamin protein.](http://images.novusbio.com/fullsize/Dynamin-Antibody-Western-Blot-NB100-61094-img0001.jpg)
![Western Blot: MDR1/ABCB1 Antibody (6G11F8) [NBP2-37290] - Western blot analysis using ABCB1 mAb against human ABCB1 (AA: 632-693) recombinant protein. (Expected MW is 32.4 kDa)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-6G11F8-Western-Blot-NBP2-37290-img0002.jpg "Western Blot: MDR1/ABCB1 Antibody (6G11F8) [NBP2-37290] - Western blot analysis using ABCB1 mAb against human ABCB1 (AA: 632-693) recombinant protein. (Expected MW is 32.4 kDa)")
![Western Blot: MDR1/ABCB1 Antibody (6G11F8) [NBP2-37290] - Western blot analysis using ABCB1 mAb against HEK293 (1) and ABCB1 (AA: 632-693)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-6G11F8-Western-Blot-NBP2-37290-img0003.jpg)
![ELISA: MDR1/ABCB1 Antibody (6G11F8) [NBP2-37290] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-6G11F8-ELISA-NBP2-37290-img0001.jpg)
![Western Blot: MDR1/ABCB1 Antibody (6G11C12) [NBP2-37294] - Analysis using ABCB1 mAb against HEK293 (1) and ABCB1 (AA: 632-693)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-6G11C12-Western-Blot-NBP2-37294-img0003.jpg "Western Blot: MDR1/ABCB1 Antibody (6G11C12) [NBP2-37294] - Analysis using ABCB1 mAb against HEK293 (1) and ABCB1 (AA: 632-693)-hIgGFc transfected HEK293 (2) cell lysate.")
![Western Blot: MDR1/ABCB1 Antibody (6G11C12) [NBP2-37294] - Analysis using ABCB1 mAb against human ABCB1 (AA: 632-693) recombinant protein. (Expected MW is 32.4 kDa).](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-6G11C12-Western-Blot-NBP2-37294-img0002.jpg)
![ELISA: MDR1/ABCB1 Antibody (6G11C12) [NBP2-37294] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-6G11C12-ELISA-NBP2-37294-img0001.jpg)
![Immunohistochemistry-Paraffin: Clathrin light chain Antibody [NBP2-97249] - Immunochemical staining of human Clathrin light chain in human placenta with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Clathrin-light-chain-Antibody-Immunohistochemistry-Paraffin-NBP2-97249-img0002.jpg "Immunohistochemistry-Paraffin: Clathrin light chain Antibody [NBP2-97249] - Immunochemical staining of human Clathrin light chain in human placenta with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.")
![Immunocytochemistry/Immunofluorescence: Clathrin light chain Antibody [NBP2-97249] - Immunofluorescence staining of Clathrin light chain in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97249 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97249_rabbit-polyclonal-clathrin-light-chain-antibody-23520251544328.png)
![Western Blot: HSP70/HSPA1A Antibody (PD01-65) [NBP3-32445] - Western blot analysis of HSP70/HSPA1A on different lysates with Rabbit anti-HSP70/HSPA1A antibody (NBP3-32445) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate Lane 2: HEK-293 cell lysate Lane 3: HepG2 cell lysate Lane 4: A431 cell lysate Lane 5: A549 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32445) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32445_rabbit-hsp70-hspa1a-mab-pd01-65-278202416331233.jpg "Western Blot: HSP70/HSPA1A Antibody (PD01-65) [NBP3-32445] - Western blot analysis of HSP70/HSPA1A on different lysates with Rabbit anti-HSP70/HSPA1A antibody (NBP3-32445) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate Lane 2: HEK-293 cell lysate Lane 3: HepG2 cell lysate Lane 4: A431 cell lysate Lane 5: A549 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32445) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HSP70/HSPA1A Antibody (PD01-65) [NBP3-32445] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-HSP70/HSPA1A antibody (NBP3-32445) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32445) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32445_rabbit-hsp70-hspa1a-mab-pd01-65-298202413585585.jpg)
![Western Blot: Ran Antibody (JE33-28) [NBP3-32892] - Western blot analysis of Ran on different lysates with Rabbit anti-Ran antibody (NBP3-32892) at 1/1,000 dilution. Lane 1: A549 cell lysate (15 ug/Lane) Lane 2: HeLa cell lysate (15 ug/Lane) Lane 3: HepG2 cell lysate (15 ug/Lane) Lane 4: LoVo cell lysate (15 ug/Lane) Lane 5: Jurkat cell lysate (15 ug/Lane) Lane 6: MDA-MB-231 cell lysate (15 ug/Lane) Lane 7: MCF7 cell lysate (15 ug/Lane) Lane 8: RAW264.7 cell lysate (15 ug/Lane) Lane 9: NIH/3T3 cell lysate (15 ug/Lane) Lane 10: Rat thymus tissue lysate (30 ug/Lane) Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32892) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32892_rabbit-ran-mab-je33-28-288202412442969.jpg "Western Blot: Ran Antibody (JE33-28) [NBP3-32892] - Western blot analysis of Ran on different lysates with Rabbit anti-Ran antibody (NBP3-32892) at 1/1,000 dilution. Lane 1: A549 cell lysate (15 ug/Lane) Lane 2: HeLa cell lysate (15 ug/Lane) Lane 3: HepG2 cell lysate (15 ug/Lane) Lane 4: LoVo cell lysate (15 ug/Lane) Lane 5: Jurkat cell lysate (15 ug/Lane) Lane 6: MDA-MB-231 cell lysate (15 ug/Lane) Lane 7: MCF7 cell lysate (15 ug/Lane) Lane 8: RAW264.7 cell lysate (15 ug/Lane) Lane 9: NIH/3T3 cell lysate (15 ug/Lane) Lane 10: Rat thymus tissue lysate (30 ug/Lane) Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32892) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: Ran Antibody (JE33-28) [NBP3-32892] - Flow cytometric analysis of HeLa cells labeling Ran. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32892, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32892_rabbit-ran-mab-je33-28-392024884630.jpg)
![Immunoprecipitation: Importin beta/KPNB1 Antibody (PSH01-59) [NBP3-32510] - Importin beta/KPNB1 was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32510 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32510 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-32510 in HeLa cell lysate Lane 3: NBP3-32510 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds](http://images.novusbio.com/fullsize/nbp3-32510_rabbit-importin-beta-kpnb1-mab-psh01-59-2682024158260.jpg "Immunoprecipitation: Importin beta/KPNB1 Antibody (PSH01-59) [NBP3-32510] - Importin beta/KPNB1 was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32510 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32510 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-32510 in HeLa cell lysate Lane 3: NBP3-32510 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds")
![Western Blot: Importin beta/KPNB1 Antibody (PSH01-59) [NBP3-32510] - Western blot analysis of Importin beta/KPNB1 on different lysates with Rabbit anti-Importin beta/KPNB1 antibody (NBP3-32510) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (20 ug/Lane) Lane 4: A549 cell lysate (20 ug/Lane) Lane 5: Jurkat cell lysate (20 ug/Lane) Lane 6: K-562 cell lysate (20 ug/Lane) Lane 7: Caco-2 cell lysate (20 ug/Lane) Lane 8: NIH/3T3 cell lysate (20 ug/Lane) Lane 9: PC-12 cell lysate (20 ug/Lane) Lane 10: C6 cell lysate (20 ug/Lane) Lane 11: Mouse spleen tissue lysate (40 ug/Lane) Lane 12: Mouse testis tissue lysate (40 ug/Lane) Lane 13: Rat spleen tissue lysate (40 ug/Lane) Lane 14: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 97 kDa Observed band size: 97 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32510) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32510_rabbit-importin-beta-kpnb1-mab-psh01-59-278202415295597.jpg)
![Western Blot: LAMP-2/CD107b Antibody (D3-D8-D3-R) [NBP3-32523] - Western blot analysis of LAMP-2/CD107b on different lysates with Mouse anti-LAMP-2/CD107b antibody (NBP3-32523) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: HepG2 cell lysate Lane 4: HUVEC cell lysate Lane 5: JAR cell lysate Lane 6: HEK-293 cell lysate Lane 7: THP-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 45 kDa Observed band size: 110 kDa Exposure time: 3 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32523) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32523_mouse-lamp-2-cd107b-mab-d3-d8-d3-r-278202416331251.jpg "Western Blot: LAMP-2/CD107b Antibody (D3-D8-D3-R) [NBP3-32523] - Western blot analysis of LAMP-2/CD107b on different lysates with Mouse anti-LAMP-2/CD107b antibody (NBP3-32523) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: HepG2 cell lysate Lane 4: HUVEC cell lysate Lane 5: JAR cell lysate Lane 6: HEK-293 cell lysate Lane 7: THP-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 45 kDa Observed band size: 110 kDa Exposure time: 3 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32523) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LAMP-2/CD107b Antibody (D3-D8-D3-R) [NBP3-32523] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-LAMP-2/CD107b antibody (NBP3-32523) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32523) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32523_mouse-lamp-2-cd107b-mab-d3-d8-d3-r-298202414304116.jpg)
![Flow Cytometry: Rat Monoclonal Integrin alpha 4/CD49d Antibody (R1-2) [NBP3-09131] - Flow cytometry surface staining pattern of murine peripheral blood cells stained using anti-mouse CD49d (R1-2) purified antibody (concentration in sample 5 ug/ml, DAR APC).](http://images.novusbio.com/fullsize/antibody/nbp3-09131_rat-monoclonal-integrin-alpha-4-cd49d-antibody-r1-2-flow-cytometry-1212202414288..jpg "Flow Cytometry: Rat Monoclonal Integrin alpha 4/CD49d Antibody (R1-2) [NBP3-09131] - Flow cytometry surface staining pattern of murine peripheral blood cells stained using anti-mouse CD49d (R1-2) purified antibody (concentration in sample 5 ug/ml, DAR APC).")
![Flow Cytometry - Rat Monoclonal Integrin alpha 4/CD49d Antibody (R1-2) [NBP3-09131] - Separation of cells stained using anti-mouse CD49d (R1-2) purified antibody (concentration in sample 5 μg/ml, DAR APC, red-filled) from cells unstained by primary antibody (DAR APC, black-dashed) in flow cytometry analysis (surface staining) of murine peripheral blood cells.](http://images.novusbio.com/fullsize/antibody/nbp3-09131_rat-monoclonal-integrin-alpha-4-cd49d-antibody-r1-2-flow-cytometry-12122024142659..jpg)