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![Western Blot: Rabbit Tau mAb (HL2982) [NBP3-45159] - Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2982] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45159_rabbit-tau-mab-hl2982-azide-and-bsa-free-western-blot-152025112952..jpg "Western Blot: Rabbit Tau mAb (HL2982) [NBP3-45159] - Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2982] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Rabbit Tau mAb (HL2982) [NBP3-45159] - Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2982] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45159_rabbit-tau-mab-hl2982-azide-and-bsa-free-western-blot-15202511328..jpg)
![Western Blot: Rabbit Tau mAb (HL2982) - Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2982] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45159_rabbit-tau-mab-hl2982-azide-and-bsa-free-western-blot-15202511382..jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Rabbit Tau mAb (HL2982) [NBP3-45159] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2982] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based.](http://images.novusbio.com/fullsize/nbp3-45159_rabbit-tau-mab-hl2982-azide-and-bsa-free-western-blot-152025115512..jpg)
![Immunocytochemistry/Immunofluorescence: MAP2 Antibody (HL1655) - Azide and BSA Free [NBP3-25554] - MAP2 antibody [HL1655] detects MAP2 protein in dendrite/axon, by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAP2 stained by MAP2 antibody [HL1655] (NBP3-25554) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25554_rabbit-map2-mab-hl1655-azide-and-bsa-free-2120249403852.jpg "Immunocytochemistry/Immunofluorescence: MAP2 Antibody (HL1655) - Azide and BSA Free [NBP3-25554] - MAP2 antibody [HL1655] detects MAP2 protein in dendrite/axon, by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAP2 stained by MAP2 antibody [HL1655] (NBP3-25554) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Immunohistochemistry-Paraffin: MAP2 Antibody (HL1655) - Azide and BSA Free [NBP3-25554] - MAP2 antibody [HL1655] detects MAP2 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. MAP2 stained by MAP2 antibody [HL1655] (NBP3-25554) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25554_rabbit-map2-mab-hl1655-azide-and-bsa-free-2120249362750.jpg)
![Immunohistochemistry-Paraffin: MAP2 Antibody (HL1655) - Azide and BSA Free [NBP3-25554] - MAP2 antibody [HL1655] detects MAP2 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. Green: MAP2 stained by MAP2 antibody [HL1655] (NBP3-25554) diluted at 1:100. Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25554_rabbit-map2-mab-hl1655-azide-and-bsa-free-2120249351398.jpg)
![<b>Orthogonal Strategies Validation. </b>MAP2 antibody [HL1655] detects MAP2 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.MAP2 stained by MAP2 antibody [HL1655] (NBP3-25554) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25554_rabbit-map2-mab-hl1655-azide-and-bsa-free-immunohistochemistry-paraffin-1411202518365923.jpg)
![Western Blot: Tau [p Ser 202, p Thr 205] Antibody (AH36) [NBP3-18265] - Western Blot analysis of Human iPSC-derived cortical neurons showing detection of Tau protein using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (NBP3-18265). Lane 1: MW ladder. Lane 2: Control (non-disease) line. Lane 2: Ex10+16 tau mutant sample. Lane 3: P301L tau mutant sample. Load: 50ug. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (NBP3-18265) at 1:500 for Overnight. pSer202/pThr205 was detected using NBP3-18265. Total tau was detected using mouse anti-tau antibody (clone HT7). The bar graph on the right shows quantification of pSer202/pThr205 compared to total tau in each sample.](http://images.novusbio.com/fullsize/Tau-p-Ser-202-p-Thr-205-Antibody-AH36-Western-Blot-NBP3-18265-img0002.jpg "Western Blot: Tau [p Ser 202, p Thr 205] Antibody (AH36) [NBP3-18265] - Western Blot analysis of Human iPSC-derived cortical neurons showing detection of Tau protein using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (NBP3-18265). Lane 1: MW ladder. Lane 2: Control (non-disease) line. Lane 2: Ex10+16 tau mutant sample. Lane 3: P301L tau mutant sample. Load: 50ug. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (NBP3-18265) at 1:500 for Overnight. pSer202/pThr205 was detected using NBP3-18265. Total tau was detected using mouse anti-tau antibody (clone HT7). The bar graph on the right shows quantification of pSer202/pThr205 compared to total tau in each sample.")
![Immunocytochemistry/Immunofluorescence: Tau [p Ser 202, p Thr 205] Antibody (AH36) [NBP3-18265] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (NBP3-18265). Tissue: iPSC-derived cortical excitatory neurons. Species: Human. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (NBP3-18265) at 1:500 for Overnight. Secondary Antibody: Donkey anti-rabbit: Alexa Fluor 488 at 1:1000. Counterstain: DAPI. A) iPSC-derived neurons from non-demented control (NDC). B) iPSC-derived neurons from subject with P301L MAPT mutation. Images acquired using an automated Opera Phoenix system. Each field of view is a max projection from 10 planes of 1 um stacks.](http://images.novusbio.com/fullsize/Tau-p-Ser-202-p-Thr-205-Antibody-AH36-Immunocytochemistry-Immunofluorescence-NBP3-18265-img0001.jpg)
![Dot Blot: Tau [p Ser 202, p Thr 205] Antibody (AH36) [NBP3-18265] - Dot Blot analysis using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (NBP3-18265). Species: E. Coli, Baculovirus. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (NBP3-18265) at 1:500. Secondary Antibody: Goat anti-rabbit IgG:HRP.](http://images.novusbio.com/fullsize/Tau-p-Ser-202-p-Thr-205-Antibody-AH36-Dot-Blot-NBP3-18265-img0003.jpg)
![Western Blot: Aurora A Antibody (4H5A8) [NBP2-61694] - Analysis using AURKA mAb against HEK293 (1) and AURKA (AA: 268-404)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-4H5A8-Western-Blot-NBP2-61694-img0003.jpg "Western Blot: Aurora A Antibody (4H5A8) [NBP2-61694] - Analysis using AURKA mAb against HEK293 (1) and AURKA (AA: 268-404)-hIgGFc transfected HEK293 (2) cell lysate.")
![Western Blot: Aurora A Antibody (4H5A8) [NBP2-61694] - Analysis using AURKA mAb against human AURKA (AA: 268-404) recombinant protein. (Expected MW is 41.5 kDa)](http://images.novusbio.com/fullsize/Aurora-A-Antibody-4H5A8-Western-Blot-NBP2-61694-img0002.jpg)
![Western Blot: Aurora A Antibody (4H5A8) [NBP2-61694] - Analysis using AURKA mouse mAb against HEK293 (1), PC-12 (2), and HT-29 (3) cell lysate.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-4H5A8-Western-Blot-NBP2-61694-img0004.jpg)
![ELISA: Aurora A Antibody (4H5A8) [NBP2-61694] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Aurora-A-Antibody-4H5A8-ELISA-NBP2-61694-img0001.jpg)
![Western Blot: JNK1/2/3 Antibody (SR1787) [NBP3-21671] - Western blot analysis using NBP3-21671 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21671_rabbit-jnk1-2-3-mab-sr1787-57202317215143.jpg "Western Blot: JNK1/2/3 Antibody (SR1787) [NBP3-21671] - Western blot analysis using NBP3-21671 in HeLa cell lysate.")
![Immunocytochemistry/Immunofluorescence: JNK1/2/3 Antibody (SR1787) [NBP3-21671] - Immunofluorescent analysis of Hela cells, using NBP3-21671.](http://images.novusbio.com/fullsize/nbp3-21671_rabbit-jnk1-2-3-mab-sr1787-113202515145432.jpg)
![Western Blot: TOR/mTOR Antibody (6611) [NBP2-43658] - Analysis of A Jurkat whole cell lysate/extract B Raji C NCI-H929 whole cell lysate/extract 5 % SDS-PAGE mTOR antibody [6611] dilution: 1:1000.](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-6611-Western-Blot-NBP2-43658-img0001.jpg "Western Blot: TOR/mTOR Antibody (6611) [NBP2-43658] - Analysis of A Jurkat whole cell lysate/extract B Raji C NCI-H929 whole cell lysate/extract 5 % SDS-PAGE mTOR antibody [6611] dilution: 1:1000.")
![Western Blot: TOR/mTOR Antibody (6611) [NBP2-43658] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 5 % SDS-PAGE mTOR antibody [6611] dilution: 1:500](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-6611-Western-Blot-NBP2-43658-img0002.jpg)
![TOR/mTOR Antibody (6611) [NBP2-43658] -HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: mTOR stained by mTOR antibody [6611] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/nbp2-43658_mouse-monoclonal-tor-mtor-antibody-6611-immunocytochemistry-immunofluorescence-15202313627..jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [GT6611] (NBP2-43658) diluted at 1:500.](http://images.novusbio.com/fullsize/nbp2-43658_mouse-monoclonal-tor-mtor-antibody-6611-western-blot-26112025182363.jpg)
![Western Blot: MAP4K6 Antibody [NBP1-32766] - Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/MAP4K6-Antibody-Western-Blot-NBP1-32766-img0001.jpg "Western Blot: MAP4K6 Antibody [NBP1-32766] - Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000")
![Immunocytochemistry/Immunofluorescence: MAP4K6 Antibody [NBP1-32766] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: MAP4K6 protein stained by MAP4K6 antibody [C2C3-2], C-term diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/MAP4K6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32766-img0005.jpg)
![Immunohistochemistry-Paraffin: MAP4K6 Antibody [NBP1-32766] - Paraffin-embedded U87 xenograft; antibody dilution 1:500.](http://images.novusbio.com/fullsize/MAP4K6-Antibody-Immunohistochemistry-Paraffin-NBP1-32766-img0004.jpg)
![Western Blot: p14ARF/CDKN2A Antibody [NB200-159] - Western blot detection of p14ARF uding NB200-159. Samples: WCE (25 ug) from BT549, positive control and U205 negative control and transfected IMR90 cells.](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-Western-Blot-NB200-159-img0002.jpg "Western Blot: p14ARF/CDKN2A Antibody [NB200-159] - Western blot detection of p14ARF uding NB200-159. Samples: WCE (25 ug) from BT549, positive control and U205 negative control and transfected IMR90 cells.")
![Immunohistochemistry: p14ARF/CDKN2A Antibody [NB200-159] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- p14ARF/p16INK4a used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-Immunohistochemistry-NB200-159-img0003.jpg)
![Immunohistochemistry-Paraffin: p14ARF/CDKN2A Antibody [NB200-159] - FFPE section of human prostate adenocarcinoma. Affinity purified rabbit anti-p14ARF used at a dilution of 1:250.](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-Immunohistochemistry-Paraffin-NB200-159-img0001.jpg)
![Western Blot: p14ARF/CDKN2A Antibody [NB200-158] - Samples : Whole cell lysate (25 ug) from BT549 (positive control), U2OS (negative control) and p14ARF/p16INK4a transfected IMR90 cells. Antibody : Affinity purified rabbit anti-p14ARF/p16INK4a used at 1 ug/ml. Detection : Chemiluminescence.](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-Western-Blot-NB200-158-img0002.jpg "Western Blot: p14ARF/CDKN2A Antibody [NB200-158] - Samples : Whole cell lysate (25 ug) from BT549 (positive control), U2OS (negative control) and p14ARF/p16INK4a transfected IMR90 cells. Antibody : Affinity purified rabbit anti-p14ARF/p16INK4a used at 1 ug/ml. Detection : Chemiluminescence.")
![Immunohistochemistry: p14ARF/CDKN2A Antibody [NB200-158] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- p14ARF/p16INK4a used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-Immunohistochemistry-NB200-158-img0003.jpg)
![Immunoprecipitation: MAP1B Antibody [NB100-68256] - Detection of human MAP1B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MAP1B antibody NB100-68256 (lot NB100-68256-2) used for IP at 6 ug per reaction. MAP1B was also immunoprecipitated by a previous lot of this antibody (lot NB100-68256-1) and another rabbit anti-MAP1B antibody. For blotting immunoprecipitated MAP1B, NB100-68256 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds](http://images.novusbio.com/fullsize/MAP1B-Antibody-Immunoprecipitation-NB100-68256-img0003.jpg "Immunoprecipitation: MAP1B Antibody [NB100-68256] - Detection of human MAP1B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MAP1B antibody NB100-68256 (lot NB100-68256-2) used for IP at 6 ug per reaction. MAP1B was also immunoprecipitated by a previous lot of this antibody (lot NB100-68256-1) and another rabbit anti-MAP1B antibody. For blotting immunoprecipitated MAP1B, NB100-68256 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds")
![Immunohistochemistry-Paraffin: CD45 Antibody (E19-G) [NBP1-30145] - Expression in lymphoid cells of the lymph node using formalin fixed, paraffin embedded human tissues. Staining with CD45 antibody (E19-G) [NBP1-30145].](http://images.novusbio.com/fullsize/CD45-Antibody-E19-G-Immunohistochemistry-Paraffin-NBP1-30145-img0005.jpg "Immunohistochemistry-Paraffin: CD45 Antibody (E19-G) [NBP1-30145] - Expression in lymphoid cells of the lymph node using formalin fixed, paraffin embedded human tissues. Staining with CD45 antibody (E19-G) [NBP1-30145].")
![Immunohistochemistry-Paraffin: CD45 Antibody (E19-G) [NBP1-30145] - Expression in lymphoid cells of the palatine tonsil using formalin fixed, paraffin embedded human tissues. Staining with CD45 antibody (E19-G) [NBP1-30145].](http://images.novusbio.com/fullsize/CD45-Antibody-E19-G-Immunohistochemistry-Paraffin-NBP1-30145-img0003.jpg)