| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20898 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55093-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81091-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92769-0.02ml | |||
![Immunoprecipitation: MEK1 Antibody [NBP3-14688] - : Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HeLa cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-MEK1recombinant monoclonal antibody used for IP at 20 ul/mg lysate. MEK1 was alsoimmunoprecipitated by rabbit anti-MEK1 recombinantmonoclonal antibody. For blottingimmunoprecipitated MEK1, was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MEK1-Antibody-Immunoprecipitation-NBP3-14688-img0001.jpg "Immunoprecipitation: MEK1 Antibody [NBP3-14688] - : Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HeLa cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-MEK1recombinant monoclonal antibody used for IP at 20 ul/mg lysate. MEK1 was alsoimmunoprecipitated by rabbit anti-MEK1 recombinantmonoclonal antibody. For blottingimmunoprecipitated MEK1, was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.")
![Immunocytochemistry/Immunofluorescence: DMAP1 Antibody [NBP2-55093] - Staining of human cell line HEK 293 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/DMAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55093-img0001.jpg "Immunocytochemistry/Immunofluorescence: DMAP1 Antibody [NBP2-55093] - Staining of human cell line HEK 293 shows localization to nucleoplasm & cytosol.")
![Western Blot: MEK2 Antibody (6D5K1) [NBP3-15651] - Western blot analysis of extracts of various cell lines, using MEK2 antibody (NBP3-15651) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/MEK2-Antibody-6D5K1-Western-Blot-NBP3-15651-img0002.jpg "Western Blot: MEK2 Antibody (6D5K1) [NBP3-15651] - Western blot analysis of extracts of various cell lines, using MEK2 antibody (NBP3-15651) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![<b>Genetic Strategies Validation. </b>Western Blot: MEK2 Antibody (6D5K1) [NBP3-15651] - Western blot analysis of extracts from normal (control) and MEK2 knockout (KO) HeLa cells, using MEK2 antibody (NBP3-15651) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/MEK2-Antibody-6D5K1-Knockout-Validated-NBP3-15651-img0001.jpg)
![Western Blot: MEK1 [p Ser218, p Ser222] Antibody [NB500-139] - Western Blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45kDa MEK 1/2 protein phosphorylated at Ser218 and Ser222. The cells were either serum starved (Control) or incubated in the presence of serum (Serum). Immunolabeling of an additional band at ~95kDa was also observed.](http://images.novusbio.com/fullsize/MEK1-[p-Ser218-p-Ser222]-Antibody-Western-Blot-NB500-139-img0001.jpg "Western Blot: MEK1 [p Ser218, p Ser222] Antibody [NB500-139] - Western Blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45kDa MEK 1/2 protein phosphorylated at Ser218 and Ser222. The cells were either serum starved (Control) or incubated in the presence of serum (Serum). Immunolabeling of an additional band at ~95kDa was also observed.")
![Western Blot: p38 alpha Antibody [NBP3-23232] - Western blot analysis of p38 MAP kinase in mouse macrophage (J774A.1) cell lysate (lanes 1-4). The blots were probed with mouse monoclonal anti-p38 alpha (C-terminal) at 1:500 (lane 1) and 1:2000 (lane 2) or rabbit polyclonal anti-p38 alpha (a.a. 319-328) at 1:250 (lane 3) and 1:1000 (lane 4).](http://images.novusbio.com/fullsize/nbp3-23232_rabbit-p38-alpha-pab-199202312403713.jpg "Western Blot: p38 alpha Antibody [NBP3-23232] - Western blot analysis of p38 MAP kinase in mouse macrophage (J774A.1) cell lysate (lanes 1-4). The blots were probed with mouse monoclonal anti-p38 alpha (C-terminal) at 1:500 (lane 1) and 1:2000 (lane 2) or rabbit polyclonal anti-p38 alpha (a.a. 319-328) at 1:250 (lane 3) and 1:1000 (lane 4).")
![Western Blot: MAP4K6 Antibody [NBP1-22988] - Whole cell lysate from HeLa and mouse NIH3T3 cells. NBP1-22988 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.](http://images.novusbio.com/fullsize/MAP4K6-Antibody-Western-Blot-NBP1-22988-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: MAP4K6 Antibody [NBP1-22988] - Whole cell lysate from HeLa and mouse NIH3T3 cells. NBP1-22988 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.")
![Western Blot: RSK1 [p Ser380] Antibody [NBP3-13243] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RSK1 (phospho Ser380) antibody (NBP3-13243) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RSK1-[p-Ser380]-Antibody-Western-Blot-NBP3-13243-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: RSK1 [p Ser380] Antibody [NBP3-13243] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RSK1 (phospho Ser380) antibody (NBP3-13243) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: RSK1 [p Ser380] Antibody [NBP3-13243] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RSK1 (phospho Ser380) antibody (NBP3-13243) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RSK1-[p-Ser380]-Antibody-Western-Blot-NBP3-13243-img0001.jpg)
![Western Blot: Rabbit Tau [p Thr217] mAb (HL1276) - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (p Thr217) antibody [HL1276] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45156_rabbit-tau-p-thr217-mab-hl1276-azide-and-bsa-free-western-blot-2942025165210..jpg "Western Blot: Rabbit Tau [p Thr217] mAb (HL1276) - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (p Thr217) antibody [HL1276] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![ELISA: Rabbit Tau [p Thr217] mAb (HL1276) [NBP3-45156] - Indirect ELISA analysis was performed by coating the plate with Tau peptide and Tau (p Thr217) peptide (1000-15.63 ng/mL). Coated peptides were probed with Tau (p Thr217) antibody [HL1276] (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-45156_rabbit-tau-p-thr217-mab-hl1276-azide-and-bsa-free-elisa-2942025164816..jpg)
![Western Blot: AMPK alpha 1 [p Ser485] Antibody - Azide and BSA Free [AMPK alpha 1] - Western blot analysis of lysates from C6 cells using AMPK alpha 1 Rabbit pAb at 1:2000 dilution. C6 cells were treated by lambdapp(20uL/400ul) at 37C for 1 hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-05462_rabbit-polyclonal-ampk-alpha-1-p-ser485-antibody-52202516334154.jpg "Western Blot: AMPK alpha 1 [p Ser485] Antibody - Azide and BSA Free [AMPK alpha 1] - Western blot analysis of lysates from C6 cells using AMPK alpha 1 Rabbit pAb at 1:2000 dilution. C6 cells were treated by lambdapp(20uL/400ul) at 37C for 1 hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.")
![Western Blot: DMAP1 Antibody [NBP2-92769] - Analysis of extracts of various cell lines, using DMAP1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.](http://images.novusbio.com/fullsize/DMAP1-Antibody-Western-Blot-NBP2-92769-img0001.jpg "Western Blot: DMAP1 Antibody [NBP2-92769] - Analysis of extracts of various cell lines, using DMAP1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.")