| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98881-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99331-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26394-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26152-50ul | |||
![Western Blot: p16INK4a/CDKN2A Antibody [NBP2-98881] - Anti-p16INK4a/CDKN2A rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 17 kDa](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP2-98881-img0005.jpg "Western Blot: p16INK4a/CDKN2A Antibody [NBP2-98881] - Anti-p16INK4a/CDKN2A rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 17 kDa")
![Immunoprecipitation: p16INK4a/CDKN2A Antibody [NBP2-98881] - p16INK4a/CDKN2A was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate 2 ul anti-p16INK4a/CDKN2A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-p16INK4a/CDKN2A rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 17 kDa](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Immunoprecipitation-NBP2-98881-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: p16INK4a/CDKN2A Antibody [NBP2-98881] - Immunofluorescence staining of p16INK4a/CDKN2A in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98881 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-98881_rabbit-polyclonal-p16ink4a-cdkn2a-antibody-235202512205526.png)
![Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32689] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Rabbit anti-p16INK4a/CDKN2A antibody (NBP3-32689) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32689) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32689_rabbit-p16ink4a-cdkn2a-mab-pd01-16-278202416331268.jpg "Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32689] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Rabbit anti-p16INK4a/CDKN2A antibody (NBP3-32689) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32689) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32689] - Immunohistochemical analysis of paraffin-embedded human ovary carcinoma tissue with Rabbit anti-p16INK4a/CDKN2A antibody (NBP3-32689) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32689) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32689_rabbit-p16ink4a-cdkn2a-mab-pd01-16-298202414333625.jpg)
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32689] - Immunocytochemistry analysis of SKOV-3 cells labeling p16INK4a/CDKN2A with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32689) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32689) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32689_rabbit-p16ink4a-cdkn2a-mab-pd01-16-29202410484819.jpg)
![Western Blot: Tau [p Ser404] Antibody (JE66-82) [NBP3-32808] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32808) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32808) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32808_rabbit-tau-p-ser404-mab-je66-82-288202412461623.jpg "Western Blot: Tau [p Ser404] Antibody (JE66-82) [NBP3-32808] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32808) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32808) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Tau [p Ser404] Antibody (JE66-82) [NBP3-32808] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Tau antibody (NBP3-32808) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32808) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32808_rabbit-tau-p-ser404-mab-je66-82-298202414262664.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody [NBP2-99331] - Immunochemical staining of human MKK4/MEK4 in human skeletal muscle with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-Immunohistochemistry-Paraffin-NBP2-99331-img0003.jpg "Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody [NBP2-99331] - Immunochemical staining of human MKK4/MEK4 in human skeletal muscle with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody [NBP2-99331] - Immunochemical staining of human MKK4/MEK4 in human breast carcinoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-Immunohistochemistry-Paraffin-NBP2-99331-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody [NBP2-99331] - Immunofluorescence staining of MKK4/MEK4 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99331 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-99331_rabbit-polyclonal-mkk4-mek4-antibody-23520252145179.png)
![Western Blot: MAP2 Antibody (MT-01) [NB500-415] - Fig. 1. Western Blotting analysis (reducing conditions) of microtubules partially purified from porcine brain lysate. Lane 1: immunostaining with anti-MAP2ab (MT-01) Lane 2: immunostaining with anti-MAP2ab (MT-07) Lane 3: immunostaining with anti-MAP2ab (MT-08). Observed molecular weight is approximately 270 kDA.](http://images.novusbio.com/fullsize/MAP2-Antibody-MT-01-Western-Blot-NB500-415-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: MAP2 Antibody (MT-01) [NB500-415] - Fig. 1. Western Blotting analysis (reducing conditions) of microtubules partially purified from porcine brain lysate. Lane 1: immunostaining with anti-MAP2ab (MT-01) Lane 2: immunostaining with anti-MAP2ab (MT-07) Lane 3: immunostaining with anti-MAP2ab (MT-08). Observed molecular weight is approximately 270 kDA.")
![Western Blot: Tau [p Ser404] Antibody (JE47-88) [NBP3-32809] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32809) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 50-70 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32809) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32809_rabbit-tau-p-ser404-mab-je47-88-288202412461654.jpg "Western Blot: Tau [p Ser404] Antibody (JE47-88) [NBP3-32809] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32809) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 50-70 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32809) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Tau [p Ser404] Antibody (JE47-88) [NBP3-32809] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Tau antibody (NBP3-32809) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32809) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32809_rabbit-tau-p-ser404-mab-je47-88-29820241492670.jpg)
![Western Blot: ERK1/2 Antibody (B20-U) [NBP1-30128] - Left Lane: Marker, Right Lane: Rat brain crude lysate (100ug of protein loaded).](http://images.novusbio.com/fullsize/ERK1-2-Antibody-B20-U-Western-Blot-NBP1-30128-img0001.jpg "Western Blot: ERK1/2 Antibody (B20-U) [NBP1-30128] - Left Lane: Marker, Right Lane: Rat brain crude lysate (100ug of protein loaded).")
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (B20-U) [NBP1-30128] - Expression in HEK293 cells.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-B20-U-Immunocytochemistry-Immunofluorescence-NBP1-30128-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: p38 alpha Antibody (M548) [NBP3-23234] - Immunocytochemical labeling of p38 alpha in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-p38 alpha (NBP3-23234). The antibody was detected using goat anti-mouse DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23234_mouse-p38-alpha-mab-m548-199202318202923.jpg "Immunocytochemistry/Immunofluorescence: p38 alpha Antibody (M548) [NBP3-23234] - Immunocytochemical labeling of p38 alpha in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-p38 alpha (NBP3-23234). The antibody was detected using goat anti-mouse DyLight(R) 594.")
![Western Blot: p38 alpha Antibody (M548) [NBP3-23234] - Western blot analysis of human A431 cells (lanes 1 & 2) and rat A7r5 cells (lanes 3 & 4). The blot was probed with mouse monoclonal anti-p38 alpha (C-terminal) clone M548 at a dilution of 1:250 (lanes 1 & 3) or 1:1000 (lanes 2 & 4).](http://images.novusbio.com/fullsize/nbp3-23234_mouse-p38-alpha-mab-m548-19920231856916.jpg)
![Immunoprecipitation: RSK1 [p Thr359, p Ser363] Antibody (1B6) [NBP3-26394] - Immunoprecipitating RSK1 [p Thr359, p Ser363] in Hela whole cell lysate. Lane 1: Rabbit control IgG (1ug) instead of NBP3-26394 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26394 (3ug) + Hela whole cell lysate (1mg). Lane 3: Hela whole cell lysate (20ug).](http://images.novusbio.com/fullsize/nbp3-26394_rabbit-rsk1-p-thr-359-p-ser-363-mab-1b6-262202413221523.jpg "Immunoprecipitation: RSK1 [p Thr359, p Ser363] Antibody (1B6) [NBP3-26394] - Immunoprecipitating RSK1 [p Thr359, p Ser363] in Hela whole cell lysate. Lane 1: Rabbit control IgG (1ug) instead of NBP3-26394 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26394 (3ug) + Hela whole cell lysate (1mg). Lane 3: Hela whole cell lysate (20ug).")
![<b>Biological Strategies Validation. </b>Western Blot: RSK1 [p Thr359, p Ser363] Antibody (1B6) [NBP3-26394] - Positive Western Blot detected in HepG2 whole cell lysate (treated with EGF or not). All lanes: RSK1 [p Thr359, p Ser363] Antibody at 0.69ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 90 KDa Observed band size: 90 KDa](http://images.novusbio.com/fullsize/nbp3-26394_rabbit-rsk1-p-thr-359-p-ser-363-mab-1b6-28220241141366.jpg)
![Immunocytochemistry/Immunofluorescence: RSK1 [p Thr359, p Ser363] Antibody (1B6) [NBP3-26394] - Staining of Hela cells with RSK1 [p Thr359, p Ser363] Antibody (1B6) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26394_rabbit-rsk1-p-thr-359-p-ser-363-mab-1b6-2822024131220.jpg)
![Immunoprecipitation: MEK1 Antibody (3F10) [NBP3-26152] - Immunoprecipitating MEK1 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26152 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26152 (2ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).](http://images.novusbio.com/fullsize/nbp3-26152_rabbit-mek1-mab-3f10-2622024132373.jpg "Immunoprecipitation: MEK1 Antibody (3F10) [NBP3-26152] - Immunoprecipitating MEK1 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26152 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26152 (2ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).")
![Immunohistochemistry: MEK1 Antibody (3F10) [NBP3-26152] - Image of MEK1 Antibody (3F10) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26152_rabbit-mek1-mab-3f10-27220248573314.jpg)
![Western Blot: MEK1 Antibody (3F10) [NBP3-26152] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, U87 whole cell lysate. All lanes: MEK1 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 44, 41 kDa Observed band size: 44 kDa](http://images.novusbio.com/fullsize/nbp3-26152_rabbit-mek1-mab-3f10-2822024114090.jpg)
![Western Blot: MAPKAPK2 Antibody (4H8) [NBP3-15173] - Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate, THP-1 whole cell lysate. All lanes: MAPKAPK2 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 46, 43 KDa. Observed band size: 49 KDa.](http://images.novusbio.com/fullsize/MAPKAPK2-Antibody-4H8-Western-Blot-NBP3-15173-img0003.jpg "Western Blot: MAPKAPK2 Antibody (4H8) [NBP3-15173] - Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate, THP-1 whole cell lysate. All lanes: MAPKAPK2 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 46, 43 KDa. Observed band size: 49 KDa.")
![Immunohistochemistry: MAPKAPK2 Antibody (4H8) [NBP3-15173] - IHC image of MAPKAPK2 antibody diluted at 1:100 and staining in paraffin-embedded human heart tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/MAPKAPK2-Antibody-4H8-Immunohistochemistry-NBP3-15173-img0002.jpg)
![Immunohistochemistry: MAPKAPK2 Antibody (4H8) [NBP3-15173] - IHC image of MAPKAPK2 antibody diluted at 1:100 and staining in paraffin-embedded human breast cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/MAPKAPK2-Antibody-4H8-Immunohistochemistry-NBP3-15173-img0001.jpg)