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![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys27] Antibody (C.15200184) [NBP2-59302] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-C-15200184-Immunofluorescence-NBP2-59302-img0003.jpg "Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys27] Antibody (C.15200184) [NBP2-59302] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys27] Antibody (C.15200184) [NBP2-59302] - ChIP assays were performed using human HeLa cells, the antibody against H3K27ac and optimized PCR primer pairs for qPCR. ChIP was performed with the Auto Histone ChIP-seq kit, using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoters of the EIF4A2 and GAPDH genes, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-C-15200184-Chromatin-Immunoprecipitation-NBP2-59302-img0002.jpg)
![ELISA: Histone H3 [ac Lys27] Antibody (C.15200184) [NBP2-59302] - To test the specificity an ELISA was performed using a serial dilution of the monoclonal antibody against H3K27ac. The wells were coated with peptides containing the unmodified H3K27 region as well as the acetylated H3K27 and the acetylated H3K9. Figure shows a high specificity of the antibody for the peptide containing the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-C-15200184-ELISA-NBP2-59302-img0005.jpg)
![Western Blot: Histone H3 [Methyl Lys79] Antibody [NBP2-59210] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H3 (lane 3) using the antibody against H3K79me1. The antibody was diluted 1:200 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys79]-Antibody-Western-Blot-NBP2-59210-img0001.jpg "Western Blot: Histone H3 [Methyl Lys79] Antibody [NBP2-59210] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H3 (lane 3) using the antibody against H3K79me1. The antibody was diluted 1:200 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [Methyl Lys79] Antibody [NBP2-59210] - ChIP assays were performed using human HeLa cells, the antibody against H3K79me1 and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for a region 1 kb upstream of the promoter and the coding region of the active GAPDH gene, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys79]-Antibody-Chromatin-Immunoprecipitation-NBP2-59210-img0003.jpg)
![ELISA: Histone H3 [Methyl Lys79] Antibody [NBP2-59210] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K79me1. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:11,500.](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys79]-Antibody-ELISA-NBP2-59210-img0002.jpg)
![Western Blot: HDAC1 Antibody (2W4I5) [NBP3-15566] - Western blot analysis of extracts of various cell lines, using HDAC1 Mouse mAb (NBP3-15566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/HDAC1-Antibody-2W4I5-Western-Blot-NBP3-15566-img0002.jpg "Western Blot: HDAC1 Antibody (2W4I5) [NBP3-15566] - Western blot analysis of extracts of various cell lines, using HDAC1 Mouse mAb (NBP3-15566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Chromatin Immunoprecipitation: HDAC1 Antibody (2W4I5) [NBP3-15566] - Chromatin immunoprecipitation analysis of extracts of K-562 cells, using HDAC1 antibody (NBP3-15566) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/HDAC1-Antibody-2W4I5-Chromatin-Immunoprecipitation-NBP3-15566-img0003.jpg)
![Western Blot: Histone H3 [p Thr3] Antibody [NBP3-12929] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (phospho Thr3) antibody (NBP3-12929) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr3]-Antibody-Western-Blot-NBP3-12929-img0003.jpg "Western Blot: Histone H3 [p Thr3] Antibody [NBP3-12929] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (phospho Thr3) antibody (NBP3-12929) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: Histone H3 [p Thr3] Antibody [NBP3-12929] - Histone H3 (phospho Thr3) antibody detects Histone H3 (phospho Thr3) protein by western blot analysis. Un-treated (-) and treated (+, 0.1uM Calyculin for 30 minutes + phosphatase inhibitor) HeLa whole cell extracts (2 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (phospho Thr3) antibody (NBP3-12929) at a dilution of 1:10000.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr3]-Antibody-Western-Blot-NBP3-12929-img0001.jpg)
![Western Blot: Histone H3 [p Thr3] Antibody [NBP3-12929] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (phospho Thr3) antibody (NBP3-12929) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr3]-Antibody-Western-Blot-NBP3-12929-img0002.jpg)
![SDS-Page: Recombinant Human Cyclophilin B Protein [NBC1-18424]](http://images.novusbio.com/fullsize/Recombinant-Human-Cyclophilin-B-Protein-SDS-Page-NBC1-18424-img0002.jpg "SDS-Page: Recombinant Human Cyclophilin B Protein [NBC1-18424]")
![Western Blot: HSP60 Antibody (S02-5B7) [NBP3-19656] - Western blot detection of HSP60 in Hela cell lysates using NBP3-19656 (1:1000 diluted). Predicted band size: 61kDa. Observed band size: 60kDa.](http://images.novusbio.com/fullsize/HSP60-Antibody-S02-5B7-Western-Blot-NBP3-19656-img0002.jpg "Western Blot: HSP60 Antibody (S02-5B7) [NBP3-19656] - Western blot detection of HSP60 in Hela cell lysates using NBP3-19656 (1:1000 diluted). Predicted band size: 61kDa. Observed band size: 60kDa.")
![Immunohistochemistry-Paraffin: HSP60 Antibody (S02-5B7) [NBP3-19656] - Immunohistochemistry of HSP60 in paraffin-embedded Human lung cancer tissue using NBP3-19656 at dilution 1/50](http://images.novusbio.com/fullsize/HSP60-Antibody-S02-5B7-Immunohistochemistry-Paraffin-NBP3-19656-img0001.jpg)
![Western Blot: Histone H3 [Unmodified Lys4] Antibody (RM186) - Azide and BSA Free [NBP3-26014] - Western Blot of acid extracts of HeLa cells treated with sodium butyrate (HeLa+NaBu), treated with Nocodazole (HeLa+Noc), or non-treated; and recombinant Histone H3.1 protein, using RM186 at 1 ug/mL, showed a band of Histone H3 unmodified at Lys4.](http://images.novusbio.com/fullsize/nbp3-26014_rabbit-histone-h3-mab-rm186-azide-and-bsa-free-226202422174413.png "Western Blot: Histone H3 [Unmodified Lys4] Antibody (RM186) - Azide and BSA Free [NBP3-26014] - Western Blot of acid extracts of HeLa cells treated with sodium butyrate (HeLa+NaBu), treated with Nocodazole (HeLa+Noc), or non-treated; and recombinant Histone H3.1 protein, using RM186 at 1 ug/mL, showed a band of Histone H3 unmodified at Lys4.")
![Immunocytochemistry/ Immunofluorescence: Histone H3 [Unmodified Lys4] Antibody (RM186) - Azide and BSA Free [NBP3-26014] - Immunocytochemical staining of HeLa cells using Clone RM186 (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-26014_rabbit-histone-h3-mab-rm186-azide-and-bsa-free-22620242311013.png)
![Immunohistochemistry-Paraffin: Transferrin Antibody (TF/3001) - Azide and BSA Free [NBP2-79912] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Transferrin Mouse Monoclonal Antibody (TF/3001).](http://images.novusbio.com/fullsize/Transferrin-Antibody-TF-3001-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-79912-img0001.jpg "Immunohistochemistry-Paraffin: Transferrin Antibody (TF/3001) - Azide and BSA Free [NBP2-79912] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Transferrin Mouse Monoclonal Antibody (TF/3001).")
![Protein Array: Transferrin Antibody (TF/3001) - Azide and BSA Free [NBP2-79912] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (mAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAbto protein X is equal to 29.](http://images.novusbio.com/fullsize/Transferrin-Antibody-TF-3001-Azide-and-BSA-Free-Protein-Array-NBP2-79912-img0002.jpg)
![Western Blot: Cyclophilin B Antibody (5479) [NBP2-59782] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PPIB antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-5479-Western-Blot-NBP2-59782-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Cyclophilin B Antibody (5479) [NBP2-59782] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PPIB antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Western Blot: Cyclophilin B Antibody (5479) [NBP2-59782] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2, mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-5479-Western-Blot-NBP2-59782-img0001.jpg)
![Western Blot: Histone H3 [ac Lys27] Antibody (RM172) [NBP2-61549] - Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate, using NBP2-61549 at 1 ug/ml, showed a band of histone H3 acetylated at Lysine 27 in treated HeLa. Observed molecular weight is ~17 kDa](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-RM172-Western-Blot-NBP2-61549-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H3 [ac Lys27] Antibody (RM172) [NBP2-61549] - Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate, using NBP2-61549 at 1 ug/ml, showed a band of histone H3 acetylated at Lysine 27 in treated HeLa. Observed molecular weight is ~17 kDa")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys27] Antibody (RM172) - Azide and BSA Free [NBP2-61549] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM172 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61549_rabbit-monoclonal-histone-h3-ac-lys27-antibody-rm172-immunocytochemistry-immunofluorescence-189202523381724.png)