| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25998 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25982 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26007 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25985 | |||
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys26] Antibody (RM154) - Azide and BSA Free [NBP3-25989] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM154 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-25989_rabbit-histone-h3-mab-rm154-azide-and-bsa-free-22620242311080.png "Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys26] Antibody (RM154) - Azide and BSA Free [NBP3-25989] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM154 (red). Actin filaments have been labeled with fluorescein phalloidin (green).")
![Multiplex Immunoassay: Histone H3 [ac Lys26] Antibody (RM154) - Azide and BSA Free [NBP3-25989] - RM154 specifically reacts to Histone H3 acetylated at Lysine 36 (K36ac), and is not affected by the phosphorylation of neighboring Ser31. No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 56 (K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.](http://images.novusbio.com/fullsize/nbp3-25989_rabbit-histone-h3-mab-rm154-azide-and-bsa-free-22620242311036.png)
![Western Blot: Histone H3 [ac Lys26] Antibody (RM154) - Azide and BSA Free [NBP3-25989] - Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+), using RM154 at 1 ug/mL, showed a band of histone H3 acetylated at Lysine 36 in treated HeLa.](http://images.novusbio.com/fullsize/nbp3-25989_rabbit-histone-h3-mab-rm154-azide-and-bsa-free-2462024132641.png)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [Dimethyl Lys4] Antibody (RM135) - Azide and BSA Free [NBP3-25985] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM135 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-25985_rabbit-histone-h3-mab-rm135-azide-and-bsa-free-226202422321235.png "Immunocytochemistry/ Immunofluorescence: Histone H3 [Dimethyl Lys4] Antibody (RM135) - Azide and BSA Free [NBP3-25985] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM135 (red). Actin filaments have been labeled with fluorescein phalloidin (green).")
![Western Blot: Histone H3 [Dimethyl Lys4] Antibody (RM135) - Azide and BSA Free [NBP3-25985] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM135 at 0.025 ug/mL, showed a band of histone H3 dimethylated at Lysine 4 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-25985_rabbit-histone-h3-mab-rm135-azide-and-bsa-free-22620242311042.png)
![Multiplex Immunoassay: Histone H3 [Dimethyl Lys4] Antibody (RM135) - Azide and BSA Free [NBP3-25985] - RM135 specifically reacts to Histone H3 dimethylated at Lysine 4 (K4me2). No cross reactivity with monomethylated Lysine 4 (K4me1) or trimethylated Lysine 4 (K4me3), or other methylations in histone H3.](http://images.novusbio.com/fullsize/nbp3-25985_rabbit-histone-h3-mab-rm135-azide-and-bsa-free-24620241332425.png)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys4] Antibody (RM149) - Azide and BSA Free [NBP3-26000] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM149 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-26000_rabbit-histone-h3-mab-rm149-azide-and-bsa-free-2262024231101.png "Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys4] Antibody (RM149) - Azide and BSA Free [NBP3-26000] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM149 (red). Actin filaments have been labeled with fluorescein phalloidin (green).")
![Multiplex Immunoassay: Histone H3 [ac Lys4] Antibody (RM149) - Azide and BSA Free [NBP3-26000] - RM149 specifically reacts to Histone H3 acetylated at Lysine 4. No cross reactivity with non-modified Lysine 4, acetylated Lysine 9, Lysine 14, Lysine 18, Lysine 23, Lysine 27, Lysine 36, Lysine 56, lysine 79, or Lysine 122 in histone H3.](http://images.novusbio.com/fullsize/nbp3-26000_rabbit-histone-h3-mab-rm149-azide-and-bsa-free-2462024132620.png)
![Western Blot: Histone H3 [ac Lys4] Antibody (RM149) - Azide and BSA Free [NBP3-26000] - Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate, using RM149 at 0.5 ug/mL, showed a band of Histone H3 acetylated at Lysine 4 in treated HeLa.](http://images.novusbio.com/fullsize/nbp3-26000_rabbit-histone-h3-mab-rm149-azide-and-bsa-free-22620242311010.png)
![Western Blot: Histone H3 [Dimethyl Lys9] Antibody (RM151) [NBP2-61534] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61534 at 0.5 ug/ml. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys9-Antibody-RM151-Western-Blot-NBP2-61534-img0007.jpg "Western Blot: Histone H3 [Dimethyl Lys9] Antibody (RM151) [NBP2-61534] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61534 at 0.5 ug/ml. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/ Immunofluorescence: Histone H3 [Dimethyl Lys9] Antibody (RM151) - Azide and BSA Free [NBP2-61534] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM151 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61534_rabbit-monoclonal-histone-h3-dimethyl-lys9-antibody-rm151-immunocytochemistry-immunofluorescence-18920252352116.png)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys9] Antibody (RM151) - Azide and BSA Free [NBP2-61534] - ChIP performed on HeLa cells using Clone RM151 (5ug). Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/nbp2-61534_rabbit-monoclonal-histone-h3-dimethyl-lys9-antibody-rm151-chromatin-immunoprecipitation-189202523474821.png)
![Western Blot: Histone H3 [ac Lys14] Antibody (RM130) [NBP2-61532] - Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+) or, using NBP2-61532. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys14-Antibody-RM130-Western-Blot-NBP2-61532-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H3 [ac Lys14] Antibody (RM130) [NBP2-61532] - Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+) or, using NBP2-61532. Observed molecular weight is ~17 kDa.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys14] Antibody (RM130) - Azide and BSA Free [NBP2-61532] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM130 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61532_rabbit-monoclonal-histone-h3-ac-lys14-antibody-rm130-immunocytochemistry-immunofluorescence-18920252343561.png)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys14] Antibody (RM130) - Azide and BSA Free [NBP2-61532] - ChIP performed on HeLa cells using RM130, 5ug. Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/nbp2-61532_rabbit-monoclonal-histone-h3-ac-lys14-antibody-rm130-chromatin-immunoprecipitation-189202523485313.png)
![Western Blot: Histone H3 [ac Lys56] Antibody (RM179) [NBP2-61537] - Western Blot of (1) acid extracts of HeLa cells treated with sodium butyrate and (2) recombinant Histone H3.3, using NBP2-61537, showed a band of Histone H3 acetylated at Lysine 56 in treated HeLa. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys56-Antibody-RM179-Western-Blot-NBP2-61537-img0007.jpg "Western Blot: Histone H3 [ac Lys56] Antibody (RM179) [NBP2-61537] - Western Blot of (1) acid extracts of HeLa cells treated with sodium butyrate and (2) recombinant Histone H3.3, using NBP2-61537, showed a band of Histone H3 acetylated at Lysine 56 in treated HeLa. Observed molecular weight is ~16 kDa.")
![Immunohistochemistry: Histone H3 [ac Lys56] Antibody (RM179) [NBP2-61537] - Immunohistochemistry staining of HepG2 cells using NBP2-61537.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys56-Antibody-RM179-Immunohistochemistry-NBP2-61537-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys56] Antibody (RM179) - Azide and BSA Free [NBP2-61537] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM179 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61537_rabbit-monoclonal-histone-h3-ac-lys56-antibody-rm179-immunocytochemistry-immunofluorescence-18920252338178.png)
![Western Blot: Histone H3 [Dimethyl Lys4] Antibody (RM135) [NBP2-61540] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61540 at 0.025 ug/ml, showed a band of histone H3 dimethylated at Lysine 4 (K4me2) in HeLa cells. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys4-Antibody-RM135-Western-Blot-NBP2-61540-img0004.jpg "Western Blot: Histone H3 [Dimethyl Lys4] Antibody (RM135) [NBP2-61540] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61540 at 0.025 ug/ml, showed a band of histone H3 dimethylated at Lysine 4 (K4me2) in HeLa cells. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/ Immunofluorescence: Histone H3 [Dimethyl Lys4] Antibody (RM135) - Azide and BSA Free [NBP2-61540] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM135 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61540_rabbit-monoclonal-histone-h3-dimethyl-lys4-antibody-rm135-immunocytochemistry-immunofluorescence-18920252339513.png)
![Multiplex Immunoassay: Histone H3 [Dimethyl Lys4] Antibody (RM135) - Azide and BSA Free [NBP2-61540] - RM135 specifically reacts to Histone H3 dimethylated at Lysine 4 (K4me2). No cross reactivity with monomethylated Lysine 4 (K4me1) or trimethylated Lysine 4 (K4me3), or other methylations in histone H3.](http://images.novusbio.com/fullsize/nbp2-61540_rabbit-monoclonal-histone-h3-dimethyl-lys4-antibody-rm135-multiplex-immunoassay-18920252343566.png)
![Western Blot: beta-Actin Antibody (M202) [NBP3-23331] - Western blot analysis of mouse C2C12 cells probed with mouse monoclonal anti-Actin (C-terminal region) antibody at 1:1000 (lane 1), 1:2000 (lane 2), or 1:4000 (lane 3).](http://images.novusbio.com/fullsize/nbp3-23331_mouse-beta-actin-mab-m202-20720231348119.jpg%3Fv=1681426096 "Western Blot: beta-Actin Antibody (M202) [NBP3-23331] - Western blot analysis of mouse C2C12 cells probed with mouse monoclonal anti-Actin (C-terminal region) antibody at 1:1000 (lane 1), 1:2000 (lane 2), or 1:4000 (lane 3).")
![Western Blot: beta-Actin Antibody (M202) [NBP3-23331] - Western blot analysis of mouse C2C12 cells probed with mouse monoclonal anti-Actin (C-terminal region) antibody at 1:1000 (lane 1), 1:2000 (lane 2), or 1:4000 (lane 3).](http://images.novusbio.com/fullsize/nbp3-23331_mouse-beta-actin-mab-m202-19920231410116.jpg)
![Immunocytochemistry/Immunofluorescence: beta-Actin Antibody [NBP3-23319] - Immunocytochemical labeling using anti-Actin (N-terminal) and anti-Actin (Tyr-53) polyclonal antibodies in C2C12 cells control (left) or treated with pervanadate (1 mM) for 30 min (middle). The cells were fixed in paraformaldehyde and permeabilized in acetone. Both antibodies were used in the presence of blocking peptide: Actin (N-terminal) peptide or phospho-Actin (Tyr-53) peptide, respectively (right).](http://images.novusbio.com/fullsize/nbp3-23319_rabbit-beta-actin-pab-119202316331610.jpg "Immunocytochemistry/Immunofluorescence: beta-Actin Antibody [NBP3-23319] - Immunocytochemical labeling using anti-Actin (N-terminal) and anti-Actin (Tyr-53) polyclonal antibodies in C2C12 cells control (left) or treated with pervanadate (1 mM) for 30 min (middle). The cells were fixed in paraformaldehyde and permeabilized in acetone. Both antibodies were used in the presence of blocking peptide: Actin (N-terminal) peptide or phospho-Actin (Tyr-53) peptide, respectively (right).")
![Western Blot: beta-Actin Antibody [NBP3-23319] - Western blot analysis of mouse C2C12 cells untreated (lanes 1 & 3), or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with anti-Actin (N-terminal) antibody (lanes 1 & 2) or anti-Actin (Tyr-53) antibody (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23319_rabbit-beta-actin-pab-19920231915417.jpg)
![Western Blot: Histone H3 [Dimethyl Lys4] Antibody (C.15200151) [NBP2-59288] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K4me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys4-Antibody-C-15200151-Western-Blot-NBP2-59288-img0001.jpg "Western Blot: Histone H3 [Dimethyl Lys4] Antibody (C.15200151) [NBP2-59288] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K4me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys4] Antibody (C.15200151) [NBP2-59288] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys4-Antibody-C-15200151-Immunofluorescence-NBP2-59288-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys4] Antibody (C.15200151) [NBP2-59288] - ChIP assays were performed using HeLa cells, the monoclonal antibody against H3K4me2 and optimized PCR primer sets for qPCR. Chromatin was sheared with the antibody consisting of 1, 5 and 10 ug per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoter and the coding region of the GAPDH gene, and for the RPL10 and HBB promoters. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys4-Antibody-C-15200151-Chromatin-Immunoprecipitation-NBP2-59288-img0003.jpg)
![ELISA: Histone H3 [Dimethyl Lys4] Antibody (C.15200151) [NBP2-59288] - Cross reactivity of the antibody directed against H3K4me2. To test the specificity an ELISA was performed using a serial dilution of the antibody against H3K4me2. The wells were coated with peptides containing the unmodified H3K4 as well as the mono-, di- and trimethylated H3K4 and the dimethylated H3K9. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys4-Antibody-C-15200151-ELISA-NBP2-59288-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys36] Antibody (C.15200183) [NBP2-59301] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys36-Antibody-C-15200183-Immunofluorescence-NBP2-59301-img0003.jpg "Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys36] Antibody (C.15200183) [NBP2-59301] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.")
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys36] Antibody (C.15200183) [NBP2-59301] - ChIP assays were performed using human HeLa cells, the antibody against H3K36me3 and optimized PCR primer pairs for qPCR. ChIP was performed with the Auto Histone ChIP-seq kit, using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the coding regions of the active EIF2S3 and CCT5 genes, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys36-Antibody-C-15200183-Chromatin-Immunoprecipitation-NBP2-59301-img0002.jpg)
![ELISA: Histone H3 [Trimethyl Lys36] Antibody (C.15200183) [NBP2-59301] - To test the specificity an ELISA was performed using a serial dilution of the antibody against H3K36me3. The wells were coated with peptides containing the unmodified H3K36 region as well as the mono-, di- and trimethylated H3K36 and the trimethylated H3K9. Figure shows a high specificity of the antibody for the peptide containing the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys36-Antibody-C-15200183-ELISA-NBP2-59301-img0001.jpg)