| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09006-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26019 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26001 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-31925-25ul | |||
![Western Blot: Histone H3 [ac Lys27] Antibody [NBP2-59225] - Western blot was performed on whole cell (40 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K27ac. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys27]-Antibody-Western-Blot-NBP2-59225-img0001.jpg "Western Blot: Histone H3 [ac Lys27] Antibody [NBP2-59225] - Western blot was performed on whole cell (40 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K27ac. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys27] Antibody [NBP2-59225] - ChIP assays were performed using HeLa cells, the antibody against H3K27ac and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers for the promoter of the active ACTB and EIF4A2 genes, used as positive controls, and for the coding region of the inactive MYT1 and TSH2B genes, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K27 acetylation is enriched at the promoters of active genes.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys27]-Antibody-Chromatin-Immunoprecipitation-NBP2-59225-img0003.jpg)
![ELISA: Histone H3 [ac Lys27] Antibody [NBP2-59225] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K27ac in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:4,000.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys27]-Antibody-ELISA-NBP2-59225-img0002.jpg)
![Immunofluorescence: Histone H3 [ac Lys27] Antibody [NBP2-59225] - HeLa cells were stained with the antibody against H3K27ac and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K27ac antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys27]-Antibody-Immunofluorescence-NBP2-59225-img0004.jpg)
![Western Blot: Histone H3 [p Thr11] Antibody [NBP2-59274] - Western blot was performed on whole cell extracts from untreated HeLa cells (25 ug, lane 1), on histone extracts from HeLa cells treated with colcemid (15 ug, lane 2), and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3T11p . The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-Western-Blot-NBP2-59274-img0001.jpg "Western Blot: Histone H3 [p Thr11] Antibody [NBP2-59274] - Western blot was performed on whole cell extracts from untreated HeLa cells (25 ug, lane 1), on histone extracts from HeLa cells treated with colcemid (15 ug, lane 2), and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3T11p . The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [p Thr11] Antibody [NBP2-59274] - ChIP assays were performed using human HeLa cells, treated with colcemid, the antibody against H3T11p and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and 5 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for the EIF4A2 and GAPDH promoters, used as positive controls, and for the coding region of the inactive MYT1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-Chromatin-Immunoprecipitation-NBP2-59274-img0004.jpg)
![Dot Blot: Histone H3 [p Thr11] Antibody [NBP2-59274] - To test the cross reactivity of the antibody against H3T11p, a Dot Blot analysis was performed with peptides containing other histone phosphorylations and the unmodified H3T11. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-Dot-Blot-NBP2-59274-img0002.jpg)
![ELISA: Histone H3 [p Thr11] Antibody [NBP2-59274] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3T11p in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:25,700.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-ELISA-NBP2-59274-img0003.jpg)
![Immunofluorescence: Histone H3 [p Thr11] Antibody [NBP2-59274] - HeLa cells were treated with colcemid and stained with the antibody against H3T11p and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3T11p antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-Immunofluorescence-NBP2-59274-img0005.jpg)
![Western Blot: Transferrin Antibody (OTI5G2) - Azide and BSA Free [NBP2-74583] - Analysis of extracts (10ug) from 6 different cell lines by using anti-TF monoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/Transferrin-Antibody-OTI5G2-Azide-and-BSA-Free-Western-Blot-NBP2-74583-img0005.jpg "Western Blot: Transferrin Antibody (OTI5G2) - Azide and BSA Free [NBP2-74583] - Analysis of extracts (10ug) from 6 different cell lines by using anti-TF monoclonal antibody (1:1000).")
![Immunocytochemistry/Immunofluorescence: Transferrin Antibody (OTI5G2) - Azide and BSA Free [NBP2-74583] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Transferrin.](http://images.novusbio.com/fullsize/Transferrin-Antibody-OTI5G2-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74583-img0004.jpg)
![Flow Cytometry: Transferrin Antibody (OTI5G2) - Azide and BSA Free [NBP2-74583] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Transferrin antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Transferrin-Antibody-OTI5G2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-74583-img0003.jpg)
![Western Blot: Transferrin Antibody (OTI5G2) - Azide and BSA Free [NBP2-74583] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-TF monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Transferrin-Antibody-OTI5G2-Azide-and-BSA-Free-Western-Blot-NBP2-74583-img0001.jpg)
![Western Blot: Transferrin Antibody (OTI5G2) - Azide and BSA Free [NBP2-74583] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TF .](http://images.novusbio.com/fullsize/Transferrin-Antibody-OTI5G2-Azide-and-BSA-Free-Western-Blot-NBP2-74583-img0002.jpg)
![Western Blot: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - HeLa (A), HEK293 (B), and HepG2 (C) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of F2C (NBP3-09006) at 0.01 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for alpha Tubulin is ~55kDa. NBP3-09006 successfully detected human alpha Tubulin in HeLa, HEK293, and HepG2 cell lysate samples.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Western-Blot-NBP3-09006-img0002.jpg "Western Blot: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - HeLa (A), HEK293 (B), and HepG2 (C) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of F2C (NBP3-09006) at 0.01 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for alpha Tubulin is ~55kDa. NBP3-09006 successfully detected human alpha Tubulin in HeLa, HEK293, and HepG2 cell lysate samples.")
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of F2C (NBP3-09006) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (1 ug/ml), showing microtubule staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09006, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09006-img0004.jpg)
![Immunohistochemistry-Paraffin: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - Anti-Alpha Tubulin staining of paraffin embedded human cerebral cortex tissue using the rabbit-chimeric version of F2C (NBP3-09006). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP3-09006 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of axons and a few neuronal cell bodies may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Immunohistochemistry-Paraffin-NBP3-09006-img0001.jpg)
![Flow Cytometry: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - HepG2 (A), HeLa (B) and Hek293 (C) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of F2C (NBP3-09006, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Flow-Cytometry-NBP3-09006-img0003.jpg)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys4] Antibody (PSH03-71) [NBP3-32429] - Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells treated 500ng/mL TSA for 4 hours and either Histone H3 (NBP3-32429) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.](http://images.novusbio.com/fullsize/nbp3-32429_rabbit-histone-h3-ac-lys4-mab-psh03-71-26820241525100.jpg "Chromatin Immunoprecipitation: Histone H3 [ac Lys4] Antibody (PSH03-71) [NBP3-32429] - Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells treated 500ng/mL TSA for 4 hours and either Histone H3 (NBP3-32429) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.")
![Dot Blot: Histone H3 [ac Lys4] Antibody (PSH03-71) [NBP3-32429] - Dot blot analysis of Histone H3 on different proteins with Rabbit anti-Histone H3 antibody (NBP3-32429) at 1/1,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution for 1 hour at room temperature. Lane 1: Unmodified Histone H3 (negative) Lane 2: Mono-Methyl-Histone H3 (Lys4) (negative) Lane 3: Di-Methyl-Histone H3 (Lys4) (negative) Lane 4: Tri-Methyl-Histone H3 (Lys4) (negative) Lane 5: Acetyl-Histone H3 (Lys4) (positive) Lane 6: Acetyl-Histone H3 (Lys9) (negative) Lane 7: Acetyl-Histone H3 (Lys27) (negative) Proteins loading: 100ng, 25ng, 5ng; Blocking and dilution buffer: 5% NFDM/TBST; Exposure time: 50 seconds.](http://images.novusbio.com/fullsize/nbp3-32429_rabbit-histone-h3-ac-lys4-mab-psh03-71-28820241459250.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys4] Antibody (PSH03-71) [NBP3-32429] - Immunocytochemistry analysis of HeLa cells treated with or without 500ng/mL TSA for 4 hours labeling Histone H3 with Rabbit anti-Histone H3 antibody (NBP3-32429) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Histone H3 antibody (NBP3-32429) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32429_rabbit-histone-h3-ac-lys4-mab-psh03-71-2920241192347.jpg)
![Western Blot: Histone H3 [ac Lys4] Antibody (PSH03-71) [NBP3-32429] - Western blot analysis of Histone H3 on different lysates with Rabbit anti-Histone H3 antibody (NBP3-32429) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 500ng/mL TSA for 4 hours cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 400nM TSA for 18 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 1 minute 22 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32429_rabbit-histone-h3-ac-lys4-mab-psh03-71-3920248453547.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys9] Antibody (RM151) - Azide and BSA Free [NBP3-25998] - ChIP performed on HeLa cells using Clone RM151 (5ug). Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/nbp3-25998_rabbit-histone-h3-mab-rm151-azide-and-bsa-free-24620241332472.png "Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys9] Antibody (RM151) - Azide and BSA Free [NBP3-25998] - ChIP performed on HeLa cells using Clone RM151 (5ug). Real-time PCR was performed using primers specific to the gene indicated.")
![Multiplex Immunoassay: Histone H3 [Dimethyl Lys9] Antibody (RM151) - Azide and BSA Free [NBP3-25998] - RM151 specifically reacts to Histone H3 dimethylated at Lysine 9 (K9me2). No cross reactivity with non-modified Lysine 9 (H3 1-19), monomethylated Lysine 9 (K9me1), trimethylated Lysine 9 (K9me3), or other methylations in Histone H3.](http://images.novusbio.com/fullsize/nbp3-25998_rabbit-histone-h3-mab-rm151-azide-and-bsa-free-22620242311096.png)
![Western Blot: Histone H3 [Dimethyl Lys9] Antibody (RM151) - Azide and BSA Free [NBP3-25998] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM151 at 0.5 ug/mL, showed a band of histone H3 dimethylated at Lysine 9 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-25998_rabbit-histone-h3-mab-rm151-azide-and-bsa-free-22620242232120.png)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [Dimethyl Lys9] Antibody (RM151) - Azide and BSA Free [NBP3-25998] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM151 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-25998_rabbit-histone-h3-mab-rm151-azide-and-bsa-free-2462024124593.png)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys79] Antibody (RM156) - Azide and BSA Free [NBP3-25990] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM156 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-25990_rabbit-histone-h3-mab-rm156-azide-and-bsa-free-2462024133242.png "Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys79] Antibody (RM156) - Azide and BSA Free [NBP3-25990] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM156 (red). Actin filaments have been labeled with fluorescein phalloidin (green).")
![Multiplex Immunoassay: Histone H3 [ac Lys79] Antibody (RM156) - Azide and BSA Free [NBP3-25990] - RM156 specifically reacts to Histone H3 acetylated at Lysine 79 (K79ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), or Lysine 122 (K122) in Histone H3.](http://images.novusbio.com/fullsize/nbp3-25990_rabbit-histone-h3-mab-rm156-azide-and-bsa-free-2462024132628.png)
![Western Blot: Histone H3 [ac Lys79] Antibody (RM156) - Azide and BSA Free [NBP3-25990] - Western Blot of acid extracts from HeLa cells untreated (-) or treated with Sodium Butyrate (+), using RM156 at 1 ug/mL.](http://images.novusbio.com/fullsize/nbp3-25990_rabbit-histone-h3-mab-rm156-azide-and-bsa-free-2262024231100.png)
![Sandwich ELISA: Histone H3 [ac Lys79] Antibody (RM156) - Azide and BSA Free [NBP3-25990] - Sandwich ELISA against acetylated histone H3 at Lys 79 using Clone RM156 (5 ug/mL) as the capture antibody and another biotinylated Histone H3 (1 ug/mL) as the detection antibody.](http://images.novusbio.com/fullsize/nbp3-25990_rabbit-histone-h3-mab-rm156-azide-and-bsa-free-22620242311033.png)
![Multiplex Immunoassay: Histone H3 [ac Lys56] Antibody (RM179) - Azide and BSA Free [NBP3-26007] - RM179 specifically reacts to Histone H3 acetylated at Lysine 56 (K56ac). No cross reactivity with unmodified Lysine 56 (K56 ctrl), acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.](http://images.novusbio.com/fullsize/nbp3-26007_rabbit-histone-h3-mab-rm179-azide-and-bsa-free-22620242311057.png "Multiplex Immunoassay: Histone H3 [ac Lys56] Antibody (RM179) - Azide and BSA Free [NBP3-26007] - RM179 specifically reacts to Histone H3 acetylated at Lysine 56 (K56ac). No cross reactivity with unmodified Lysine 56 (K56 ctrl), acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.")
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys56] Antibody (RM179) - Azide and BSA Free [NBP3-26007] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM179 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-26007_rabbit-histone-h3-mab-rm179-azide-and-bsa-free-24620241245921.png)
![Immunohistochemistry-Paraffin: Histone H3 [ac Lys56] Antibody (RM179) - Azide and BSA Free [NBP3-26007] - Immunohistochemistry staining of HepG2 cells using Clone RM179.](http://images.novusbio.com/fullsize/nbp3-26007_rabbit-histone-h3-mab-rm179-azide-and-bsa-free-2462024132630.png)
![Western Blot: Histone H3 [ac Lys56] Antibody (RM179) - Azide and BSA Free [NBP3-26007] - Western Blot of (1) acid extracts of HeLa cells treated with sodium butyrate and (2) recombinant Histone H3.3, using RM179 at 1 ug/mL, showed a band of Histone H3 acetylated at Lysine 56 in treated HeLa.](http://images.novusbio.com/fullsize/nbp3-26007_rabbit-histone-h3-mab-rm179-azide-and-bsa-free-22620242311024.png)
![Multiplex Immunoassay: Histone H3 [Monomethyl Lys9] Antibody (RM150) - Azide and BSA Free [NBP3-26001] - RM150 specifically reacts to Histone H3 monomethylated at Lysine 9 (K9me1). No cross reactivity with non-modified Lysine 9, dimethylated Lysine 9 (K9me2), trimethylated Lysine 4 (K9me3), or other methylations in histone H3.](http://images.novusbio.com/fullsize/nbp3-26001_rabbit-histone-h3-mab-rm150-azide-and-bsa-free-22620242232121.png "Multiplex Immunoassay: Histone H3 [Monomethyl Lys9] Antibody (RM150) - Azide and BSA Free [NBP3-26001] - RM150 specifically reacts to Histone H3 monomethylated at Lysine 9 (K9me1). No cross reactivity with non-modified Lysine 9, dimethylated Lysine 9 (K9me2), trimethylated Lysine 4 (K9me3), or other methylations in histone H3.")
![Western Blot: Histone H3 [Monomethyl Lys9] Antibody (RM150) - Azide and BSA Free [NBP3-26001] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM150 at 0. 5 ug/mL, showed a band of histone H3 monomethylated at Lysine 9 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-26001_rabbit-histone-h3-mab-rm150-azide-and-bsa-free-2462024132634.png)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [Monomethyl Lys9] Antibody (RM150) - Azide and BSA Free [NBP3-26001] - Immunocytochemistry of HeLa cells, using Clone RM150 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-26001_rabbit-histone-h3-mab-rm150-azide-and-bsa-free-2462024124594.png)
![Chromatin Immunoprecipitation: Histone H3 [Monomethyl Lys9] Antibody (RM150) - Azide and BSA Free [NBP3-26001] - ChIP performed on HeLa cells using Clone RM150 (5ug). Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/nbp3-26001_rabbit-histone-h3-mab-rm150-azide-and-bsa-free-chromatin-immunoprecipitation-2462024225540..png)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [p Thr3] Antibody (RM159) - Azide and BSA Free [NBP2-61546] - Immunocytochemistry of HeLa cells using Clone RM159 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61546_rabbit-monoclonal-histone-h3-p-thr3-antibody-rm159-immunocytochemistry-immunofluorescence-18920252353230.png "Immunocytochemistry/ Immunofluorescence: Histone H3 [p Thr3] Antibody (RM159) - Azide and BSA Free [NBP2-61546] - Immunocytochemistry of HeLa cells using Clone RM159 (red). Actin filaments have been labeled with fluorescein phalloidin (green).")
![Histone H3 [p Thr3] Antibody (RM159) - Azide and BSA Free [NBP2-61546] - Immunocytochemistry of HeLa cells using NBP3-26019 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61546_rabbit-monoclonal-histone-h3-p-thr3-antibody-rm159-immunocytochemistry-immunofluorescence-189202523485321.png)
![Immunohistochemistry-Paraffin: Histone H3 [p Thr3] Antibody (RM159) [NBP2-61546] - Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue section using NBP2-61546 at 1 ug/mL.](http://images.novusbio.com/fullsize/nbp2-61546_rabbit-monoclonal-histone-h3-p-thr3-antibody-rm159-131020231935490.png)
![Western Blot: Histone H3 [Monomethyl Lys9] Antibody (RM150) [NBP2-61535] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61535 at 0.5 ug/ml. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Monomethyl-Lys9-Antibody-RM150-Western-Blot-NBP2-61535-img0007.jpg "Western Blot: Histone H3 [Monomethyl Lys9] Antibody (RM150) [NBP2-61535] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61535 at 0.5 ug/ml. Observed molecular weight is ~16 kDa.")
![Immunocytochemistry/ Immunofluorescence: Histone H3 [Monomethyl Lys9] Antibody (RM150) - Azide and BSA Free [NBP2-61535] - Immunocytochemistry of HeLa cells, using Clone RM150 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61535_rabbit-monoclonal-histone-h3-monomethyl-lys9-antibody-rm150-immunocytochemistry-immunofluorescence-189202523444720.png)
![Multiplex Immunoassay: Histone H3 [Monomethyl Lys9] Antibody (RM150) - Azide and BSA Free [NBP2-61535] - RM150 specifically reacts to Histone H3 monomethylated at Lysine 9 (K9me1). No cross reactivity with non-modified Lysine 9, dimethylated Lysine 9 (K9me2), trimethylated Lysine 4 (K9me3), or other methylations in histone H3.](http://images.novusbio.com/fullsize/nbp2-61535_rabbit-monoclonal-histone-h3-monomethyl-lys9-antibody-rm150-multiplex-immunoassay-189202523474823.png)
![Chromatin Immunoprecipitation: Histone H3 [Monomethyl Lys9] Antibody (RM150) - Azide and BSA Free [NBP2-61535] - ChIP performed on HeLa cells using Clone RM150 (5ug). Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/nbp2-61535_rabbit-monoclonal-histone-h3-monomethyl-lys9-antibody-rm150-chromatin-immunoprecipitation-189202523394315.png)
![Immunocytochemistry/Immunofluorescence: GAPDH-2 Antibody [NBP2-31925] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-31925-img0010.jpg "Immunocytochemistry/Immunofluorescence: GAPDH-2 Antibody [NBP2-31925] - Staining of human cell line U-2 OS shows localization to nucleoplasm.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GAPDH-2 Antibody [NBP2-31925] - Staining in human testis and kidney tissues using anti-GAPDHS antibody. Corresponding GAPDHS RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Immunohistochemistry-Paraffin-NBP2-31925-img0009.jpg)
![Immunohistochemistry-Paraffin: GAPDH-2 Antibody [NBP2-31925] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Immunohistochemistry-Paraffin-NBP2-31925-img0007.jpg)
![Immunohistochemistry-Paraffin: GAPDH-2 Antibody [NBP2-31925] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Immunohistochemistry-Paraffin-NBP2-31925-img0008.jpg)