| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-148SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76798SS | |||
| Azide and BSA Free | NBP2-80879 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74051 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35155-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-39029-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22619-50ul | |||
![Western Blot: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - NPC proteins in mouse lungs. Western blot of wild type (W) littermates, NPC1 (Mut) or NPC2 (Mut) mutant mouse lungs using anti-NPC1 or -NPC2 antibody. beta-actin used as a loading control. 30 ug protein/lane. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0067084), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody---BSA-Free-Western-Blot-NB400-148-img0015.jpg "Western Blot: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - NPC proteins in mouse lungs. Western blot of wild type (W) littermates, NPC1 (Mut) or NPC2 (Mut) mutant mouse lungs using anti-NPC1 or -NPC2 antibody. beta-actin used as a loading control. 30 ug protein/lane. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0067084), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - NPC1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB400-148-img0010.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - Staining of human brain, cortex, neurons and astrocytes.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB400-148-img0011.jpg)
![Western Blot: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - Protein expression analysis of normal and NPC-deficient cells after HIV-1 infection. Cells were uninfected or infected with VSVG-HIV-1 and harvested 96 h post-infection. NPC2, NPC1, and beta-actin protein expression was detected via Western blotting in uninfected and infected cells. Gag expression was also detected in the infected cells. Image collected and cropped by CiteAb from the following publication (//virologyj.biomedcentral.com/articles/10.1186/1743-422X-9-31), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody---BSA-Free-Western-Blot-NB400-148-img0016.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - Immunocytochemical staining with corresponding colocalization analysis of ALIX (green) and NPC-1 (red) in fibroblasts exposed to LLOMe for 15 min. Nuclei are stained with DAPI (blue) and merged images show colocalization in yellow (n = 5). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32409651/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Niemann-Pick C1 Antibody - BSA Free-Immunocytochemistry-Immunofluorescence-NB400-148-img0017.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NPC1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human NPC1 Polyclonal Antibody (Catalog # NB400-148) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for NPC1 at approximately 240-260 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody---BSA-Free-Knockout-Validated-NB400-148-img0014.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - HeLa lysate, BHK lysate, A431 lysate tested at 1:1000. Image from verified customer review.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody---BSA-Free-Knockdown-Validated-NB400-148-img0012.jpg)
![Immunocytochemistry/ Immunofluorescence: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - ESCRT complex recruited to LLOMe-damaged lysosomes promotes cell survival.Human fibroblasts were exposed to 1 mM LLOMe & when indicated, the cell permeable calcium chelator BAPTA-AM (1 µM) was added 10 min prior to LLOMe exposure. Immunocytochemical staining with corresponding colocalization analysis of a CHMP4B (green) & LAMP2 (red), b ALIX (green) & NPC-1 (red), & c CHMP4B (green) & LAMP2 (red) in fibroblasts exposed to LLOMe for 15 min. Nuclei are stained with DAPI (blue) & merged images show colocalization in yellow (n = 5). d Caspase-3 like activity in cells exposed to LLOMe for 8 h (n = 3). e Quantification of apoptotic & necrotic cells using Annexin V/PI staining in fibroblasts exposed to LLOMe for 6 h (n = 3). f Immunoblot of LAMP2 in digitonin-extracted cytosolic fractions from cells exposed to LLOMe for 2 h (n = 8) with corresponding quantitative analysis. *p < 0.05, **p < 0.01 & ***p < 0.001 compared to control (a–b) or LLOMe only (c–f). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32409651), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-148_rabbit-polyclonal-niemann-pick-c1-antibody-310202416165630.jpg)
![Western Blot: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - Expression of proteins involved in extracellular lipid uptake & intracellular cholesterol trafficking following JQ1 (0.4 µM) administration to HepG2 cells for 48 hours. (A) Representative Western blot (left panel) & densitometric analysis (right panel) of SR-B1. n = 6 independent experiments. (B) Immunofluorescence analysis of SR-B1 (green). Nuclei were counterstained with DAPI. n = 3 different experiments. Scale bar: 50 µm. (C) Representative Western blot (left panel) & densitometric analysis (right panel) of LDLr. n = 5 independent experiments. (D) LDLr immunofluorescence (green). Nuclei were counterstained with DAPI. n = 3 different experiments. (E–F) Representative Western blots & densitometric analysis of NPC1 & TMEM97. Tubulin was chosen as loading control. n = 6 independent experiments. Data represent means ± SD. Statistical analysis was performed by using unpaired Student’s t test. ** p < 0.01; *** p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32075110), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-148_rabbit-polyclonal-niemann-pick-c1-antibody-310202416163786.jpg)
![Western Blot: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - Effects of p75NTR modulation by LM11A-31 on cholesterol metabolism & secretion. (A–D) Representative Western blot & densitometric analysis of HMGCR, ABCA1, NPC1, & ApoE in U373 cells treated with vehicle (Ctrl) & LM11A-31 (0.1 µM) for 48 h. n = 4 different experiments. Vinculin served as a housekeeping protein to normalize protein loading. (E) Quantification of ApoE amount (µg/mL) by ELISA assay in culture medium from vehicle- & LM11A-31-treated U373 cells. n = 3 different experiments. (F) Cholesterol quantification (total cholesterol, free cholesterol, & cholesteryl esters) in the culture medium of U373 cells treated with vehicle (Ctrl) & LM11A-31 (0.1 µM) for 48 h. n = 3 different experiments. Data represent means ± SD. Statistical analysis was carried out by using unpaired Student’s t test. *** p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35563230), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-148_rabbit-polyclonal-niemann-pick-c1-antibody-31020241616058.jpg)
![Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Niemann-Pick Type C1/NPC1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (Catalog # MAB10105) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C1/NPC1 at approximately 170-250 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Knockout-Validated-NBP2-76798-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Niemann-Pick Type C1/NPC1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (Catalog # MAB10105) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C1/NPC1 at approximately 170-250 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 for 5 minutes. The cells were incubated with anti- Niemann-Pick C1 Antibody at 2 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunocytochemistry-Immunofluorescence-NBP2-76798-img0006.jpg)
![Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Total protein from human HeLa, Hek293, mouse 3T3 and Rat PC12 cell lines were separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NPC1 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Western-Blot-NBP2-76798-img0005.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Niemann-Pick Type C1/NPC1 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (NBP2-76798) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic puncta.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunohistochemistry-Paraffin-NBP2-76798-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Niemann-Pick C1 Antibody (1318A) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunocytochemistry-Immunofluorescence-NBP2-76798-img0004.jpg)
![Western Blot: SEC14L2 Antibody (4H2) [NBP2-01346] Analysis of extracts (35ug) from 9 different cell lines by using anti-SEC14L2 monoclonal antibody.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-4H2-Western-Blot-NBP2-01346-img0006.jpg "Western Blot: SEC14L2 Antibody (4H2) [NBP2-01346] Analysis of extracts (35ug) from 9 different cell lines by using anti-SEC14L2 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: SEC14L2 Antibody (4H2) [NBP2-01346] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SEC14L2.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-4H2-Immunocytochemistry-Immunofluorescence-NBP2-01346-img0005.jpg)
![Immunohistochemistry-Paraffin: SEC14L2 Antibody (4H2) [NBP2-01346] - Staining of paraffin-embedded Human prostate tissue using anti-SEC14L2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-4H2-Immunohistochemistry-Paraffin-NBP2-01346-img0004.jpg)
![Flow Cytometry: SEC14L2 Antibody (4H2) [NBP2-01346] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SEC14L2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-4H2-Flow-Cytometry-NBP2-01346-img0001.jpg)
![Western Blot: SEC14L2 Antibody (4H2) [NBP2-01346] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SEC14L2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SEC14L2.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-4H2-Western-Blot-NBP2-01346-img0002.jpg)
![Immunohistochemistry-Paraffin: SEC14L2 Antibody (4H2) [NBP2-01346] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SEC14L2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-4H2-Immunohistochemistry-Paraffin-NBP2-01346-img0003.jpg)
![Immunohistochemistry: SEC14L2 Antibody [NBP3-35155] - Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using SEC14L2 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35155_rabbit-sec14l2-pab-912202415133421.jpg "Immunohistochemistry: SEC14L2 Antibody [NBP3-35155] - Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using SEC14L2 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: SEC14L2 Antibody [NBP3-35155] - Immunohistochemistry analysis of paraffin-embedded Mouse pancreas using SEC14L2 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35155_rabbit-sec14l2-pab-912202415121312.jpg)
![Immunohistochemistry: SEC14L2 Antibody [NBP3-35155] - Immunohistochemistry analysis of paraffin-embedded Rat lung using SEC14L2 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35155_rabbit-sec14l2-pab-912202414201353.jpg)
![Immunocytochemistry/ Immunofluorescence: SEC14L2 Antibody [NBP3-35155] - Immunofluorescence analysis of U-2 OS cells using SEC14L2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35155_rabbit-sec14l2-pab-912202415175267.jpg)
![Western Blot: SEC14L2 Antibody [NBP3-35155] - Western blot analysis of various lysates using SEC14L2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35155_rabbit-sec14l2-pab-912202415152860.jpg)
![Western Blot: Niemann-Pick C1 Antibody (4H2) [H00004864-M02] - NPC1 monoclonal antibody (M02), clone 4H2. Analysis of NPC1 expression in HeLa.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-4H2-Western-Blot-H00004864-M02-img0003.jpg "Western Blot: Niemann-Pick C1 Antibody (4H2) [H00004864-M02] - NPC1 monoclonal antibody (M02), clone 4H2. Analysis of NPC1 expression in HeLa.")
![ELISA: Niemann-Pick C1 Antibody (4H2) [H00004864-M02] - Detection limit for recombinant GST tagged NPC1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-4H2-ELISA-H00004864-M02-img0004.jpg)
![Western Blot: SEC14L2 Antibody (JE61-74) [NBP3-32935] - Western blot analysis of SEC14L2 on different lysates with Rabbit anti-SEC14L2 antibody (NBP3-32935) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 16 seconds. 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32935) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32935_rabbit-sec14l2-mab-je61-74-288202412442940.jpg "Western Blot: SEC14L2 Antibody (JE61-74) [NBP3-32935] - Western blot analysis of SEC14L2 on different lysates with Rabbit anti-SEC14L2 antibody (NBP3-32935) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 16 seconds. 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32935) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SEC14L2 Antibody (JE61-74) [NBP3-32935] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-SEC14L2 antibody (NBP3-32935) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32935) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32935_rabbit-sec14l2-mab-je61-74-298202414262627.jpg)
![Immunocytochemistry/ Immunofluorescence: SEC14L2 Antibody (JE61-74) [NBP3-32935] - Immunocytochemistry analysis of A549 cells labeling SEC14L2 with Rabbit anti-SEC14L2 antibody (NBP3-32935) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SEC14L2 antibody (NBP3-32935) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32935_rabbit-sec14l2-mab-je61-74-29202410234211.jpg)
![Western Blot: SEC14L2 Antibody [NBP2-15112] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-Western-Blot-NBP2-15112-img0002.jpg "Western Blot: SEC14L2 Antibody [NBP2-15112] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE, antibody diluted at 1:500.")
![Immunohistochemistry-Paraffin: SEC14L2 Antibody [NBP2-15112] - Paraffin-embedded H1299 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-Immunohistochemistry-Paraffin-NBP2-15112-img0001.jpg)
![SEC14L2 antibody [N1C1] detects SEC14L2 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SEC14L2 protein stained by SEC14L2 antibody [N1C1] (NBP2-15112) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-15112_rabbit-polyclonal-sec14l2-antibody-immunocytochemistry-immunofluorescence-261120251849136.jpg)
![Immunocytochemistry/Immunofluorescence: SEC14L2 Antibody [NBP2-39029] - Staining of human cell line A-431 shows localization to nucleus & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-39029-img0002.jpg "Immunocytochemistry/Immunofluorescence: SEC14L2 Antibody [NBP2-39029] - Staining of human cell line A-431 shows localization to nucleus & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: SEC14L2 Antibody [NBP2-39029] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-Immunohistochemistry-Paraffin-NBP2-39029-img0004.jpg)
![Immunohistochemistry-Paraffin: SEC14L2 Antibody [NBP2-39029] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-Immunohistochemistry-Paraffin-NBP2-39029-img0003.jpg)
![Western Blot: SEC14L2 Antibody (SR1665) [NBP3-22619] - Western blot analysis using NBP3-22619 in U87-MG cell lysate.](http://images.novusbio.com/fullsize/nbp3-22619_rabbit-sec14l2-mab-sr1665-6720239533748.jpg "Western Blot: SEC14L2 Antibody (SR1665) [NBP3-22619] - Western blot analysis using NBP3-22619 in U87-MG cell lysate.")
![Immunocytochemistry/Immunofluorescence: SEC14L2 Antibody (2E5) [H00023541-M05] - Analysis of monoclonal antibody to SEC14L2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/SEC14L2-Antibody-2E5-Immunocytochemistry-Immunofluorescence-H00023541-M05-img0001.jpg "Immunocytochemistry/Immunofluorescence: SEC14L2 Antibody (2E5) [H00023541-M05] - Analysis of monoclonal antibody to SEC14L2 on HeLa cell. Antibody concentration 10 ug/ml")
![Sandwich ELISA: SEC14L2 Antibody (2E5) [H00023541-M05] - Detection limit for recombinant GST tagged SEC14L2 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-2E5-Sandwich-ELISA-H00023541-M05-img0002.jpg)
![Western Blot: Niemann-Pick C1 Antibody [NBP2-26214] - Staining of HEK293 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-Western-Blot-NBP2-26214-img0001.jpg "Western Blot: Niemann-Pick C1 Antibody [NBP2-26214] - Staining of HEK293 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")