| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-42176-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41221-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-25266 | |||
| Azide and BSA Free | NBP2-25266SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48501-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83107-25ul | |||
![Western Blot: ATF4 Antibody (S360A-24) [NBP2-42176] - Western Blot analysis of Rat Brain showing detection of ~39 kDa (isoform 2) ATF4protein using Mouse Anti-ATF4Monoclonal Antibody, Clone S360A-24 (NBP2-42176). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-ATF4Monoclonal Antibody (NBP2-42176) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~39 kDa (isoform 2).](http://images.novusbio.com/fullsize/ATF4-Antibody-S360A-24-Western-Blot-NBP2-42176-img0004.jpg "Western Blot: ATF4 Antibody (S360A-24) [NBP2-42176] - Western Blot analysis of Rat Brain showing detection of ~39 kDa (isoform 2) ATF4protein using Mouse Anti-ATF4Monoclonal Antibody, Clone S360A-24 (NBP2-42176). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-ATF4Monoclonal Antibody (NBP2-42176) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~39 kDa (isoform 2).")
![Immunocytochemistry/Immunofluorescence: ATF4 Antibody (S360A-24) [NBP2-42176] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ATF4Monoclonal Antibody, Clone S360A-24 (NBP2-42176). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-ATF4Monoclonal Antibody (NBP2-42176) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) ATF4Antibody (D) Composite.](http://images.novusbio.com/fullsize/ATF4-Antibody-S360A-24-Immunocytochemistry-Immunofluorescence-NBP2-42176-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ATF4 Antibody (S360A-24) [NBP2-42176] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ATF4 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Cell Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize/ATF4-Antibody-S360A-24-Immunocytochemistry-Immunofluorescence-NBP2-42176-img0001.jpg)
![Immunohistochemistry: LIMPII/SR-B2 Antibody (PSH03-25) [NBP3-32538] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LIMPII/SR-B2 antibody (NBP3-32538) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32538) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32538_rabbit-limpii-sr-b2-mab-psh03-25-29820241420974.jpg "Immunohistochemistry: LIMPII/SR-B2 Antibody (PSH03-25) [NBP3-32538] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LIMPII/SR-B2 antibody (NBP3-32538) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32538) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody (PSH03-25) [NBP3-32538] - Immunocytochemistry analysis of HeLa cells labeling LIMPII/SR-B2 with Rabbit anti-LIMPII/SR-B2 antibody (NBP3-32538) at 1/200 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LIMPII/SR-B2 antibody (NBP3-32538) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32538_rabbit-limpii-sr-b2-mab-psh03-25-292024119239.jpg)
![Western Blot: LIMPII/SR-B2 Antibody (PSH03-25) [NBP3-32538] - Western blot analysis of LIMPII/SR-B2 on different lysates with Rabbit anti-LIMPII/SR-B2 antibody (NBP3-32538) at 1/5,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: HepG2 cell lysate (15 µg/Lane) Lane 3: SH-SY5Y cell lysate (15 µg/Lane) Lane 4: Neuro-2a cell lysate (15 µg/Lane) Lane 5: MEF cell lysate (15 µg/Lane) Lane 6: PC-12 cell lysate (15 µg/Lane) Lane 7: COS-1 cell lysate (15 µg/Lane) Lane 8: Mouse kidney tissue lysate (30 µg/Lane) Lane 9: Rat kidney tissue lysate (30 µg/Lane) Lane 10: Mouse liver tissue lysate (30 µg/Lane) Lane 11: Rat liver tissue lysate (30 µg/Lane) Predicted band size: 54 kDa Observed band size: 80 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32538_rabbit-limpii-sr-b2-mab-psh03-25-392024845352.jpg)
![Western Blot: GRP78/HSPA5 Antibody (C9-9-R) [NBP3-32408] - Western blot analysis of GRP78/HSPA5 on different lysates with Mouse anti-GRP78/HSPA5 antibody (NBP3-32408) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (15 ug/Lane) Lane 2: HepG2 cell lysate (15 ug/Lane) Lane 3: Mouse brain tissue lysate (30 ug/Lane) Lane 4: U-87 MG cell lysate (30 ug/Lane) Lane 5: RAW264.7 cell lysate (30 ug/Lane) Lane 6: RAW264.7 treated with 300nM Thapsigargin for 18 hours cell lysate (30 ug/Lane) Lane 7: Mouse liver tissue lysate (30 ug/Lane) Lane 8: Rat liver tissue lysate (30 ug/Lane) Lane 9: Rat pancreas tissue lysate (30 ug/Lane) Predicted band size: 72 kDa Observed band size: 72 kDa Exposure time: Lane 1-3: 11 seconds; Lane 4-9: 4 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32408) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32408_mouse-grp78-hspa5-mab-c9-9-r-278202415212468.jpg "Western Blot: GRP78/HSPA5 Antibody (C9-9-R) [NBP3-32408] - Western blot analysis of GRP78/HSPA5 on different lysates with Mouse anti-GRP78/HSPA5 antibody (NBP3-32408) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (15 ug/Lane) Lane 2: HepG2 cell lysate (15 ug/Lane) Lane 3: Mouse brain tissue lysate (30 ug/Lane) Lane 4: U-87 MG cell lysate (30 ug/Lane) Lane 5: RAW264.7 cell lysate (30 ug/Lane) Lane 6: RAW264.7 treated with 300nM Thapsigargin for 18 hours cell lysate (30 ug/Lane) Lane 7: Mouse liver tissue lysate (30 ug/Lane) Lane 8: Rat liver tissue lysate (30 ug/Lane) Lane 9: Rat pancreas tissue lysate (30 ug/Lane) Predicted band size: 72 kDa Observed band size: 72 kDa Exposure time: Lane 1-3: 11 seconds; Lane 4-9: 4 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32408) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: GRP78/HSPA5 Antibody (C9-9-R) [NBP3-32408] - Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-GRP78/HSPA5 antibody (NBP3-32408) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32408) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32408_mouse-grp78-hspa5-mab-c9-9-r-29820241411540.jpg)
![Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody (C9-9-R) [NBP3-32408] - Immunocytochemistry analysis of Hela cells labeling GRP78/HSPA5 with Mouse anti-GRP78/HSPA5 antibody (NBP3-32408) at 1/200 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-GRP78/HSPA5 antibody (NBP3-32408) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32408_mouse-grp78-hspa5-mab-c9-9-r-29202410484873.png)
![Immunocytochemistry/Immunofluorescence: ATF4 Antibody [NBP2-15499] - HeLa cells mock (left) and treated with 2 ug/ml Tunicamycin for 8hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATF4 protein stained by ATF4 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/ATF4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15499-img0003.jpg "<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ATF4 Antibody [NBP2-15499] - HeLa cells mock (left) and treated with 2 ug/ml Tunicamycin for 8hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATF4 protein stained by ATF4 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.")
![Western Blot: PRAS40 Antibody [NBP2-41221] - Western blot analysis of AKT1S1 in human brain tissue lysate with AKT1S1 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/PRAS40-Antibody-Western-Blot-NBP2-41221-img0001.jpg "Western Blot: PRAS40 Antibody [NBP2-41221] - Western blot analysis of AKT1S1 in human brain tissue lysate with AKT1S1 antibody at (A) 1 and (B) 2 ug/mL.")
![Immunohistochemistry-Paraffin: PRAS40 Antibody [NBP2-41221] - Rat brain tissue with AKT1S1 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/PRAS40-Antibody-Immunohistochemistry-Paraffin-NBP2-41221-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: PRAS40 Antibody - BSA Free [NBP2-41221] - Immunofluorescence of PRAS40 in rat brain tissue with PRAS40 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41221_rabbit-polyclonal-pras40-antibody-2711202420141913.jpg)
![Western Blot: PLA2G2A Antibody [NBP2-42399] - Analysis of Human Placenta lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PLA2G2A-Antibody-Western-Blot-NBP2-42399-img0001.jpg "Western Blot: PLA2G2A Antibody [NBP2-42399] - Analysis of Human Placenta lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: PLA2G2A Antibody [NBP2-42399] - Staining of paraffin embedded Human Small Intestine. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PLA2G2A-Antibody-Immunohistochemistry-Paraffin-NBP2-42399-img0003.jpg)
![Immunohistochemistry-Paraffin: PLA2G2A Antibody [NBP2-42399] - Staining of paraffin embedded Human Liver. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PLA2G2A-Antibody-Immunohistochemistry-Paraffin-NBP2-42399-img0002.jpg)
![Western Blot: Noxa Antibody (69N15C9) [NBP2-25251] - Western blot analysis of mouse A) Noxa recombinant protein, B) thymus and C) ovary using Noxa antibody at 1 ug/mL, 5 ug/mL and 5 ug/mL, respectively.](http://images.novusbio.com/fullsize/Noxa-Antibody-69N15C9-Western-Blot-NBP2-25251-img0001.jpg "Western Blot: Noxa Antibody (69N15C9) [NBP2-25251] - Western blot analysis of mouse A) Noxa recombinant protein, B) thymus and C) ovary using Noxa antibody at 1 ug/mL, 5 ug/mL and 5 ug/mL, respectively.")
![Western Blot: IRE1 alpha Antibody (8D12) [H00002081-M02] - IRE1 Antibody (8D12) [H00002081-M02] - ERN1 monoclonal antibody (M02), clone 8D12. Analysis of ERN1 expression in LNCaP. Theoretical molecular weight: 110 kDa.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-8D12-Western-Blot-H00002081-M02-img0008.jpg "Western Blot: IRE1 alpha Antibody (8D12) [H00002081-M02] - IRE1 Antibody (8D12) [H00002081-M02] - ERN1 monoclonal antibody (M02), clone 8D12. Analysis of ERN1 expression in LNCaP. Theoretical molecular weight: 110 kDa.")
![Immunocytochemistry/Immunofluorescence: IRE1 alpha Antibody (8D12) [H00002081-M02] - Analysis of monoclonal antibody to ERN1 on HeLa cell. Antibody concentration 25 ug/ml.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-8D12-Immunocytochemistry-Immunofluorescence-H00002081-M02-img0006.jpg)
![Western Blot: IRE1 alpha Antibody (8D12) [H00002081-M02] - Analysis of ERN1 expression in HeLa. Theoretical molecular weight: 110 kDa. Observed molecular weight may vary.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-8D12-Western-Blot-H00002081-M02-img0001.jpg)
![Proximity Ligation Assay: IRE1 alpha Antibody (8D12) [H00002081-M02] - Analysis of protein-protein interactions between TRAF2 and ERN1. HeLa cells were stained with anti-TRAF2 rabbit purified polyclonal 1:1200 and anti-ERN1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-8D12-Proximity-Ligation-Assay-H00002081-M02-img0004.jpg)
![ELISA: IRE1 alpha Antibody (8D12) [H00002081-M02] - IRE1 Antibody (8D12) [H00002081-M02] - Detection limit for recombinant GST tagged ERN1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-8D12-ELISA-H00002081-M02-img0007.jpg)
![Enzyme Activity: Glucosylceramidase/GBA Colorimetric Assay Kit [KA1611] - Kinetics of beta-glucosidase reaction in 96-well plate assay.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Colorimetric-Assay-Kit-Enzyme-Activity-KA1611-img0001.jpg "Enzyme Activity: Glucosylceramidase/GBA Colorimetric Assay Kit [KA1611] - Kinetics of beta-glucosidase reaction in 96-well plate assay.")
![Immunohistochemistry-Paraffin: ATF6 Antibody [NBP2-48501] - Staining of human testis shows moderate cytoplasmic positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/ATF6-Antibody-Immunohistochemistry-Paraffin-NBP2-48501-img0008.jpg "Immunohistochemistry-Paraffin: ATF6 Antibody [NBP2-48501] - Staining of human testis shows moderate cytoplasmic positivity in a subset of cells in seminiferous ducts.")
![Immunohistochemistry-Paraffin: ATF6 Antibody [NBP2-48501] - Staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ATF6-Antibody-Immunohistochemistry-Paraffin-NBP2-48501-img0005.jpg)
![Immunohistochemistry-Paraffin: ATF6 Antibody [NBP2-48501] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATF6-Antibody-Immunohistochemistry-Paraffin-NBP2-48501-img0006.jpg)
![Immunohistochemistry-Paraffin: ATF6 Antibody [NBP2-48501] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ATF6-Antibody-Immunohistochemistry-Paraffin-NBP2-48501-img0007.jpg)
![Western Blot: IMPA1 Antibody [NBP1-83107] - Analysis in control (vector only transfected HEK293T lysate) and IMPA1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/IMPA1-Antibody-Western-Blot-NBP1-83107-img0007.jpg "Western Blot: IMPA1 Antibody [NBP1-83107] - Analysis in control (vector only transfected HEK293T lysate) and IMPA1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: IMPA1 Antibody [NBP1-83107] - Staining of human cell line U-251 MG shows localization to nucleoplasm & mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/IMPA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83107-img0006.jpg)
![Immunohistochemistry-Paraffin: IMPA1 Antibody [NBP1-83107] - Staining of human testis shows strong cytoplasmic and nuclear positivity in seminiferous tubules.](http://images.novusbio.com/fullsize/IMPA1-Antibody-Immunohistochemistry-Paraffin-NBP1-83107-img0001.jpg)