| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-23518-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89306-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90503-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99029-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89460-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89345-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90272-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89930-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89432-50ul | |||
![Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody (0014) [NBP3-23518] - Western blot analysis of extracts from serum-starved HEK293, untreated (line A); treated with IGF1 (100ng/ml, 15min;+) (line B); treated with IGF1 and -phosphatase (line C) using NBP3-23518 at 1:5000 dilution.](http://images.novusbio.com/fullsize/nbp3-23518_rabbit-4ebp1-mab-0014-azide-and-bsa-free-235202511545617.png "Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody (0014) [NBP3-23518] - Western blot analysis of extracts from serum-starved HEK293, untreated (line A); treated with IGF1 (100ng/ml, 15min;+) (line B); treated with IGF1 and -phosphatase (line C) using NBP3-23518 at 1:5000 dilution.")
![Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody (0014) [NBP3-23518] - Western blot analysis of extracts from HEK293, untreated(line A) or treated with IGF1 (100ng/ml, 15min; +)(line B), using NBP3-23518 at 1:5000 dilution (upper) or Mouse Monoclonal 4EBP1 Antibody at 1:5000 dilution (lower).](http://images.novusbio.com/fullsize/nbp3-23518_rabbit-4ebp1-mab-0014-azide-and-bsa-free-23520251628169.png)
![Immunocytochemistry/Immunofluorescence: IGF-I R/IGF1R Antibody (012) [NBP2-89306] - Staining of Human IGF1R in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human IGF1R monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane.](http://images.novusbio.com/fullsize/IGF-I-R-IGF1R-Antibody-012-Immunocytochemistry-Immunofluorescence-NBP2-89306-img0001.jpg "Immunocytochemistry/Immunofluorescence: IGF-I R/IGF1R Antibody (012) [NBP2-89306] - Staining of Human IGF1R in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human IGF1R monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane.")
![Flow Cytometry: IGF-I R/IGF1R Antibody (012) [NBP2-89306] - Analysis of human IGF1R expression on HeLa cells. Cells were stained with purified anti-Human IGF1R, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/IGF-I-R-IGF1R-Antibody-012-Flow-Cytometry-NBP2-89306-img0002.jpg)
![Immunohistochemistry-Paraffin: S100A1 Antibody (004) [NBP2-90503] - Immunochemical staining of mouse S100A1 in mouse heart with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/S100A1-Antibody-004-Immunohistochemistry-Paraffin-NBP2-90503-img0002.jpg "Immunohistochemistry-Paraffin: S100A1 Antibody (004) [NBP2-90503] - Immunochemical staining of mouse S100A1 in mouse heart with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: S100A1 Antibody (004) [NBP2-90503] - Immunochemical staining of human mouse S100A1 in mouse bone with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to chondrocyte.](http://images.novusbio.com/fullsize/S100A1-Antibody-004-Immunohistochemistry-Paraffin-NBP2-90503-img0001.jpg)
![Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP2-99029] - Anti-Leukotriene A4 Hydrolase/LTA4H rabbit polyclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 60 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 70 kDa. Observed band size: 63 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-Western-Blot-NBP2-99029-img0002.jpg "Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP2-99029] - Anti-Leukotriene A4 Hydrolase/LTA4H rabbit polyclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 60 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 70 kDa. Observed band size: 63 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP2-99029] - Leukotriene A4 Hydrolase/LTA4H was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate. 2 ul anti-Leukotriene A4 Hydrolase/LTA4H rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Leukotriene A4 Hydrolase/LTA4H rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 69 kDa. Observed band size: 69 kDa](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-Immunoprecipitation-NBP2-99029-img0001.jpg)
![Immunohistochemistry-Paraffin: Thioredoxin-1 Antibody (214) [NBP2-89460] - Staining of human TXN in human appendix with rabbit monoclonal antibody (1:200). Positive staining was localized to intestinal gland.](http://images.novusbio.com/fullsize/Thioredoxin-1-Antibody-214-Immunohistochemistry-Paraffin-NBP2-89460-img0002.jpg "Immunohistochemistry-Paraffin: Thioredoxin-1 Antibody (214) [NBP2-89460] - Staining of human TXN in human appendix with rabbit monoclonal antibody (1:200). Positive staining was localized to intestinal gland.")
![Immunohistochemistry-Paraffin: Thioredoxin-1 Antibody (214) [NBP2-89460] - Staining of human TXN in human colon with rabbit monoclonal antibody (1:200). Positive staining was localized to colonic gland.](http://images.novusbio.com/fullsize/Thioredoxin-1-Antibody-214-Immunohistochemistry-Paraffin-NBP2-89460-img0001.jpg)
![Flow Cytometry: Leptin/OB Antibody (002) [NBP2-89345] - Analysis of Human LEP expression on HeLa cells. The cells were treated, stained with purified anti-Human LEP, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Leptin-OB-Antibody-002-Flow-Cytometry-NBP2-89345-img0001.jpg "<b>Biological Strategies Validation. </b>Flow Cytometry: Leptin/OB Antibody (002) [NBP2-89345] - Analysis of Human LEP expression on HeLa cells. The cells were treated, stained with purified anti-Human LEP, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Leptin/OB Antibody (002) [NBP2-89345] - Staining of LEP in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-89345 (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Cytoplasm .](http://images.novusbio.com/fullsize/nbp2-89345_rabbit-monoclonal-leptin-ob-antibody-002-23520251129371.png)
![Western Blot: AIF-1/Iba1 Antibody (145) [NBP2-90272] - Lane A: THP-1 Whole Cell LysateLane B: HL-60 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:17 kDaObserved band size:15 kDa](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-145-Western-Blot-NBP2-90272-img0001.jpg "Western Blot: AIF-1/Iba1 Antibody (145) [NBP2-90272] - Lane A: THP-1 Whole Cell LysateLane B: HL-60 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:17 kDaObserved band size:15 kDa")
![Immunoprecipitation: AIF-1/Iba1 Antibody (145) [NBP2-90272] - Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg THP-1 Whole Cell Lysate2 uL anti-AIF1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-AIF1 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 17 kDaObserved band size: 17 kDa](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-145-Immunoprecipitation-NBP2-90272-img0002.jpg)
![Immunohistochemistry-Paraffin: NM23-H1 Antibody (084) [NBP2-89930] - Staining of human NME1 in human hepatoma with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/NM23-H1-Antibody-084-Immunohistochemistry-Paraffin-NBP2-89930-img0002.jpg "Immunohistochemistry-Paraffin: NM23-H1 Antibody (084) [NBP2-89930] - Staining of human NME1 in human hepatoma with rabbit monoclonal antibody (1:200).")
![Immunohistochemistry-Paraffin: NM23-H1 Antibody (084) [NBP2-89930] - Staining of human NME1 in human lung cancer with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/NM23-H1-Antibody-084-Immunohistochemistry-Paraffin-NBP2-89930-img0001.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (09) - Azide and BSA Free [NBP3-26103] - Immunochemical staining of human Carbonic Anhydrase IX/CA9 in human stomach with mouse monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-26103_mouse-carbonic-anhydrase-ix-ca9-mab-09-azide-bsa-free-12620241540466.png "Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (09) - Azide and BSA Free [NBP3-26103] - Immunochemical staining of human Carbonic Anhydrase IX/CA9 in human stomach with mouse monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (09) - Azide and BSA Free [NBP3-26103] - Immunochemical staining of human Carbonic Anhydrase IX/CA9 in human renal carcinoma with mouse monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-26103_mouse-carbonic-anhydrase-ix-ca9-mab-09-azide-bsa-free-13620241428460.png)
![Immunocytochemistry/Immunofluorescence: E-Selectin/CD62E Antibody (208) [NBP2-89432] - Staining of CD62E in TNF-a stimulated HUVEC cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-89432 antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.](http://images.novusbio.com/fullsize/nbp2-89432_rabbit-monoclonal-e-selectin-cd62e-antibody-208-235202514371334.png "Immunocytochemistry/Immunofluorescence: E-Selectin/CD62E Antibody (208) [NBP2-89432] - Staining of CD62E in TNF-a stimulated HUVEC cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-89432 antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.")
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody [NBP3-23415] - Western blot image of recombinant his-tagged human Sphingosine Kinase 1/SPHK1 protein that was phosphorylated with ERK-2. Blots were probed with anti-Sphingosine Kinase 1/SPHK1 (Central Region) (NBP3-23415; lanes 1-3) and anti-Sphingosine Kinase 1/SPHK1 (Ser-225) (NBP3-23421; lanes 4-6). Both antibodies were used in the presence of no peptide (lanes 1 & 4), phospho-Sphingosine Kinase 1/SPHK1 (Ser-225) peptide (lanes 2 & 5), or unphosphorylated Sphingosine Kinase 1/SPHK1 (Ser-225) peptide (lanes 3 & 6).](http://images.novusbio.com/fullsize/nbp3-23415_rabbit-sphingosine-kinase-1-sphk1-pab-19920231410110.jpg "Western Blot: Sphingosine Kinase 1/SPHK1 Antibody [NBP3-23415] - Western blot image of recombinant his-tagged human Sphingosine Kinase 1/SPHK1 protein that was phosphorylated with ERK-2. Blots were probed with anti-Sphingosine Kinase 1/SPHK1 (Central Region) (NBP3-23415; lanes 1-3) and anti-Sphingosine Kinase 1/SPHK1 (Ser-225) (NBP3-23421; lanes 4-6). Both antibodies were used in the presence of no peptide (lanes 1 & 4), phospho-Sphingosine Kinase 1/SPHK1 (Ser-225) peptide (lanes 2 & 5), or unphosphorylated Sphingosine Kinase 1/SPHK1 (Ser-225) peptide (lanes 3 & 6).")
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody [NBP3-23415] - Western blot of HeLa stimulated with calyculin A (lanes 1-4). The blots were untreated (lane 1 & 3) or treated with lambda phosphatase (lane 2 & 4), then probed with anti-Sphingosine Kinase 1/SPHK1 (Central region) NBP3-23415 (lanes 1 & 2) or anti-Sphingosine Kinase 1/SPHK1 (Ser-225) NBP3-23421 (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23415_rabbit-sphingosine-kinase-1-sphk1-pab-19920231911474.jpg)
![Western Blot: Sphingosine Kinase 1/SPHK1 [p Ser225] Antibody [NBP3-23421] - Western blot image of recombinant his-tagged human Sphingosine Kinase 1/SPHK1 protein that was phosphorylated with ERK-2. Blots were probed with anti-Sphingosine Kinase 1/SPHK1 (Central Region) (NBP3-23415; lanes 1-3) and anti-Sphingosine Kinase 1/SPHK1 (Ser-225) (NBP3-23421; lanes 4-6). Both antibodies were used in the presence of no peptide (lanes 1 & 4), phospho-Sphingosine Kinase 1/SPHK1 (Ser-225) peptide (lanes 2 & 5), or unphosphorylated Sphingosine Kinase 1/SPHK1 (Ser-225) peptide (lanes 3 & 6).](http://images.novusbio.com/fullsize/nbp3-23421_rabbit-sphingosine-kinase-1-sphk1-p-ser225-pab-199202312523626.jpg "Western Blot: Sphingosine Kinase 1/SPHK1 [p Ser225] Antibody [NBP3-23421] - Western blot image of recombinant his-tagged human Sphingosine Kinase 1/SPHK1 protein that was phosphorylated with ERK-2. Blots were probed with anti-Sphingosine Kinase 1/SPHK1 (Central Region) (NBP3-23415; lanes 1-3) and anti-Sphingosine Kinase 1/SPHK1 (Ser-225) (NBP3-23421; lanes 4-6). Both antibodies were used in the presence of no peptide (lanes 1 & 4), phospho-Sphingosine Kinase 1/SPHK1 (Ser-225) peptide (lanes 2 & 5), or unphosphorylated Sphingosine Kinase 1/SPHK1 (Ser-225) peptide (lanes 3 & 6).")
![Western Blot: Sphingosine Kinase 1/SPHK1 [p Ser225] Antibody [NBP3-23421] - Western blot of HeLa stimulated with calyculin A (lanes 1-4). The blots were untreated (lane 1 & 3) or treated with lambda phosphatase (lane 2 & 4), then probed with anti-Sphingosine Kinase 1/SPHK1 (Central region) NBP3-23415 (lanes 1 & 2) or anti-Sphingosine Kinase 1/SPHK1 (Ser-225) NBP3-23421 (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23421_rabbit-sphingosine-kinase-1-sphk1-p-ser225-pab-1992023141010.jpg)