| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NBP3-11945-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26592-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47995-0.1mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NB100-2001-0.1mg | |||
![Western Blot: FHIT Antibody [NBP3-27889] - Western blot analysis of FHIT in HeLa cell lysate with FHIT antibody at (A) 1 and (B) 2 u/ml.](http://images.novusbio.com/fullsize/nbp3-27889_rabbit-fhit-pab-2711202420414526.gif "Western Blot: FHIT Antibody [NBP3-27889] - Western blot analysis of FHIT in HeLa cell lysate with FHIT antibody at (A) 1 and (B) 2 u/ml.")
![Immunocytochemistry/ Immunofluorescence: FHIT Antibody [NBP3-27889] - Immunofluorescence of FHIT in HeLa cells with FHIT antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp3-27889_rabbit-fhit-pab-2711202419201569.jpg)
![Western Blot: Cytochrome P450 17A1 Antibody (5D2H1) [NBP3-16541] - Western blot analysis of extracts of various cell lines, using Cytochrome P450 17A1 Rabbit mAb (NBP3-16541) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-5D2H1-Western-Blot-NBP3-16541-img0002.jpg "Western Blot: Cytochrome P450 17A1 Antibody (5D2H1) [NBP3-16541] - Western blot analysis of extracts of various cell lines, using Cytochrome P450 17A1 Rabbit mAb (NBP3-16541) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 17A1 Antibody (5D2H1) [NBP3-16541] - Immunofluorescence analysis of HeLa cells using Cytochrome P450 17A1 Rabbit mAb (NBP3-16541) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-5D2H1-Immunocytochemistry-Immunofluorescence-NBP3-16541-img0001.jpg)
![Western Blot: PI 3-Kinase p85 alpha [p Tyr199, p Tyr467] Antibody (PSH01-38) [NBP3-32818] - Western blot analysis of PI 3-Kinase p85 alpha on different lysates with Rabbit anti-PI 3-Kinase p85 alpha antibody (NBP3-32818) at 1/1,000 dilution. Lane 1: NIH/3T3 whole cell lysate Lane 2: NIH/3T3 treated with 1mM sodium orthovanadate for 30 minutes whole cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 84 kDa Observed band size: 55/85 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32818) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32818_rabbit-pi-3-kinase-p85-alpha-p-tyr199-p-tyr467-mab-288202412442996.jpg "Western Blot: PI 3-Kinase p85 alpha [p Tyr199, p Tyr467] Antibody (PSH01-38) [NBP3-32818] - Western blot analysis of PI 3-Kinase p85 alpha on different lysates with Rabbit anti-PI 3-Kinase p85 alpha antibody (NBP3-32818) at 1/1,000 dilution. Lane 1: NIH/3T3 whole cell lysate Lane 2: NIH/3T3 treated with 1mM sodium orthovanadate for 30 minutes whole cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 84 kDa Observed band size: 55/85 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32818) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: PI 3-Kinase p85 alpha [p Tyr199, p Tyr467] Antibody (PSH01-38) [NBP3-32818] - Immunocytochemistry analysis of NIH/3T3 cells treated with or without 1mM Sodium orthovanadate for 30 minutes labeling PI 3-Kinase p85 alpha with Rabbit anti-PI 3-Kinase p85 alpha antibody (NBP3-32818) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PI 3-Kinase p85 alpha antibody (NBP3-32818) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32818_rabbit-pi-3-kinase-p85-alpha-p-tyr199-p-tyr467-mab-29202410334087.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP3-11945] - Western blot of mouse liver lysate showing specific immunolabeling of the ~35, 38 kDa Arginase I/ARG1/liver Arginase protein.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Western-Blot-NBP3-11945-img0001.jpg "Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP3-11945] - Western blot of mouse liver lysate showing specific immunolabeling of the ~35, 38 kDa Arginase I/ARG1/liver Arginase protein.")
![Immunohistochemistry: FAAH Antibody (8G3) [NBP3-26592] - Image of FAAH Antibody (8G3) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26592_rabbit-faah-mab-8g3-262202415355712.jpg "Immunohistochemistry: FAAH Antibody (8G3) [NBP3-26592] - Image of FAAH Antibody (8G3) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: FAAH Antibody (8G3) [NBP3-26592] - Positive Western Blot detected in: MCF-7 whole cell lysate, THP-1 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Mouse Liver whole cell lysate. All lanes: FAAH1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 64 kDa Observed band size: 64 kDa](http://images.novusbio.com/fullsize/nbp3-26592_rabbit-faah-mab-8g3-28220241136306.jpg)
![Western Blot: FAAH Antibody [NBP2-16397] - Sample (30 ug of whole cell lysate) A: MCF-7 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/FAAH-Antibody-Western-Blot-NBP2-16397-img0001.jpg "Western Blot: FAAH Antibody [NBP2-16397] - Sample (30 ug of whole cell lysate) A: MCF-7 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: FAAH Antibody [NBP2-16397] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/FAAH-Antibody-Immunohistochemistry-Paraffin-NBP2-16397-img0002.jpg)
![Western Blot: Insulin Receptor beta Antibody [NB120-10991] - Representative lot data. Human brain lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-Insulin Receptor, b subunit (1:1,000). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Insulin Receptor, b subunit (~97kDa)](http://images.novusbio.com/fullsize/Insulin-Receptor-beta-Antibody-Western-Blot-NB120-10991-img0005.jpg "Western Blot: Insulin Receptor beta Antibody [NB120-10991] - Representative lot data. Human brain lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-Insulin Receptor, b subunit (1:1,000). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Insulin Receptor, b subunit (~97kDa)")
![Immunoprecipitation: Insulin Receptor beta Antibody [NB120-10991] - Immunoprecipitation 10 ug of this antibody immunoprecipitated a-Insulin Receptor, Beta subunit from 500 micrograms of Hek293 cell lysate using protein A agarose beads. Arrow indicates a-Insulin Receptor, Beta subunit (97 kDa).](http://images.novusbio.com/fullsize/Insulin-Receptor-beta-Antibody-Immunoprecipitation-NB120-10991-img0004.jpg)
![Western Blot: ARH Antibody [NBP2-15449] - Sample (30 ug of whole cell lysate) A: U87-MG 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/ARH-Antibody-Western-Blot-NBP2-15449-img0001.jpg "Western Blot: ARH Antibody [NBP2-15449] - Sample (30 ug of whole cell lysate) A: U87-MG 12% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: ARH Antibody [NBP2-15449] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ARH-Antibody-Immunohistochemistry-Paraffin-NBP2-15449-img0002.jpg)
![Western Blot: PFKP Antibody [NBP2-32192] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-PFKP antibody NBP2-32192 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PFKP-Antibody-Western-Blot-NBP2-32192-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: PFKP Antibody [NBP2-32192] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-PFKP antibody NBP2-32192 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: PFKP Antibody [NBP2-32192] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PFKP antibody NBP2-32192 used for IP at 6 ug per reaction. For blotting immunoprecipitated PFKP, NBP2-32192 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/PFKP-Antibody-Immunoprecipitation-NBP2-32192-img0002.jpg)
![Western Blot: alcohol dehydrogenase Antibody [NB600-912] - Analysis using Azide/BSA FREE version of NB600-912. Detection of yeast Alcohol Dehydrogenase. Comparison to molecular weight markers (not shown) indicates estimated molecular weights consistent with monomer, dimer and tetramer present in this preparation. The blot was incubated with a 1:500 dilution of the antibody at room temperature for 2 h followed by detection using 800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min at room temperature.](http://images.novusbio.com/fullsize/alcohol-dehydrogenase-Antibody-Western-Blot-NB600-912-img0006.jpg "Western Blot: alcohol dehydrogenase Antibody [NB600-912] - Analysis using Azide/BSA FREE version of NB600-912. Detection of yeast Alcohol Dehydrogenase. Comparison to molecular weight markers (not shown) indicates estimated molecular weights consistent with monomer, dimer and tetramer present in this preparation. The blot was incubated with a 1:500 dilution of the antibody at room temperature for 2 h followed by detection using 800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min at room temperature.")
![Western Blot: alcohol dehydrogenase Antibody [NB600-912] - Lane 1: Alcohol Dehydrogenase. Load: 50ng per lane. Primary antibody: Alcohol Dehydrogenase Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase Conjugated Rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 37kDa, 45kDa.](http://images.novusbio.com/fullsize/alcohol-dehydrogenase-Antibody-Western-Blot-NB600-912-img0004.jpg)
![Western Blot: alcohol dehydrogenase Antibody [NB600-912] - Analysis using Biotin conjugate of NB600-912. Lane 1: Alcohol Dehydrogenase (yeast). Load: 50 ng per lane. Primary antibody: Rabbit anti-Alcohol Dehydrogenase (Yeast) Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase Streptavidin Antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 37 kDa, 45 kDa for Alcohol Dehydrogenase (yeast).](http://images.novusbio.com/fullsize/alcohol-dehydrogenase-Antibody-Western-Blot-NB600-912-img0005.jpg)