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![Western Blot: Pyruvate Carboxylase Antibody (JE40-24) [NBP3-32721] - Western blot analysis of Pyruvate Carboxylase on different lysates with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32721) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 130 kDa Observed band size: 130 kDa Exposure time: 1 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32721) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32721_rabbit-pyruvate-carboxylase-mab-je40-24-278202416331212.jpg "Western Blot: Pyruvate Carboxylase Antibody (JE40-24) [NBP3-32721] - Western blot analysis of Pyruvate Carboxylase on different lysates with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32721) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 130 kDa Observed band size: 130 kDa Exposure time: 1 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32721) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Pyruvate Carboxylase Antibody (JE40-24) [NBP3-32721] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32721) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32721) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32721_rabbit-pyruvate-carboxylase-mab-je40-24-2982024142096.jpg)
![Western Blot: iNOS [p Tyr1055] Antibody [NBP3-23217] - Western blot analysis of mouse macrophages (J774A.1) treated with LPS (1ug/ml) for 18 hrs followed by pervanadate (1 mM) for 30 min. (lanes 1, 3 & 5). The blots were then treated with akaline phosphatase (lanes 2, 4 & 6). Blots were probed with rabbit polyclonal anti-inducible Nitric Oxide Synthase (iNOS) (lanes 1 & 2), anti-iNOS (Tyr-1055) (lanes 3 & 4), and mouse monoclonal anti-iNOS (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23217_rabbit-inos-p-tyr1055-pab-19920231759440.jpg "Western Blot: iNOS [p Tyr1055] Antibody [NBP3-23217] - Western blot analysis of mouse macrophages (J774A.1) treated with LPS (1ug/ml) for 18 hrs followed by pervanadate (1 mM) for 30 min. (lanes 1, 3 & 5). The blots were then treated with akaline phosphatase (lanes 2, 4 & 6). Blots were probed with rabbit polyclonal anti-inducible Nitric Oxide Synthase (iNOS) (lanes 1 & 2), anti-iNOS (Tyr-1055) (lanes 3 & 4), and mouse monoclonal anti-iNOS (lanes 5 & 6).")
![Immunocytochemistry/Immunofluorescence: iNOS [p Tyr1055] Antibody [NBP3-23217] - Immunocytochemical labeling of nNOS phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with mouse monoclonal (mAb) nNOS (NBP3-23224), and rabbit polyclonal (pAb) nNOS (C-terminal region), nNOS (Tyr-895)/eNOS (Tyr-657), and nNOS (Tyr-1326)/iNOS (Tyr-1055). The antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23217_rabbit-inos-p-tyr1055-pab-19920231992812.jpg)
![Western Blot: Catalase Antibody (S01-8B2) [NBP3-19593] - Western blot detection of Catalase in K562, Hela cell lysates using NBP3-19593 (1:1000 diluted). Predicted band size: 60kDa. Observed band size: 60kDa.](http://images.novusbio.com/fullsize/Catalase-Antibody-S01-8B2-Western-Blot-NBP3-19593-img0002.jpg "Western Blot: Catalase Antibody (S01-8B2) [NBP3-19593] - Western blot detection of Catalase in K562, Hela cell lysates using NBP3-19593 (1:1000 diluted). Predicted band size: 60kDa. Observed band size: 60kDa.")
![Immunohistochemistry-Paraffin: Catalase Antibody (S01-8B2) [NBP3-19593] - Immunohistochemistry of Catalase in paraffin-embedded Human breast cancer tissue using NBP3-19593 at dilution 1/100](http://images.novusbio.com/fullsize/Catalase-Antibody-S01-8B2-Immunohistochemistry-Paraffin-NBP3-19593-img0001.jpg)
![Western Blot: Catalase Antibody (S03-8E9) [NBP3-14914] - Western blot detection of Catalase in K562, Rat Brain, C6, 3T3 cell lysates using Catalase Rabbit mAb (1:1000 diluted). Predicted band size: 60kDa. Observed band size: 60kDa.](http://images.novusbio.com/fullsize/Catalase-Antibody-S03-8E9-Western-Blot-NBP3-14914-img0001.jpg "Western Blot: Catalase Antibody (S03-8E9) [NBP3-14914] - Western blot detection of Catalase in K562, Rat Brain, C6, 3T3 cell lysates using Catalase Rabbit mAb (1:1000 diluted). Predicted band size: 60kDa. Observed band size: 60kDa.")
![Immunocytochemistry/Immunofluorescence: Catalase Antibody (S03-8E9) [NBP3-14914] - Immunofluorescence of Catalase (green) in Raw264. 7 using NBP3-14914 at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-14914_rabbit-catalase-mab-s03-8e9-113202515145476.jpg)
![Immunocytochemistry/Immunofluorescence: 15-Lipoxygenase 2 Antibody (4A7) [H00000247-M05] - Analysis of monoclonal antibody to ALOX15B on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/15-Lipoxygenase-2-Antibody-4A7-Immunocytochemistry-Immunofluorescence-H00000247-M05-img0004.jpg "Immunocytochemistry/Immunofluorescence: 15-Lipoxygenase 2 Antibody (4A7) [H00000247-M05] - Analysis of monoclonal antibody to ALOX15B on HeLa cell. Antibody concentration 10 ug/ml")
![ELISA: 15-Lipoxygenase 2 Antibody (4A7) [H00000247-M05] - Detection limit for recombinant GST tagged ALOX15B is approximately 10ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-2-Antibody-4A7-ELISA-H00000247-M05-img0006.jpg)
![Immunohistochemistry-Paraffin: CYP11B2 Antibody [NBP2-49267] - Staining in human adrenal gland and skeletal muscle tissues.. Corresponding CYP11B2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CYP11B2-Antibody-Immunohistochemistry-Paraffin-NBP2-49267-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CYP11B2 Antibody [NBP2-49267] - Staining in human adrenal gland and skeletal muscle tissues.. Corresponding CYP11B2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CYP11B2 Antibody [NBP2-49267] - Staining of human adrenal gland shows moderate to strong positivity in mitochondria in glandular cells.](http://images.novusbio.com/fullsize/CYP11B2-Antibody-Immunohistochemistry-Paraffin-NBP2-49267-img0004.jpg)
![Immunohistochemistry-Paraffin: CYP11B2 Antibody [NBP2-49267] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/CYP11B2-Antibody-Immunohistochemistry-Paraffin-NBP2-49267-img0005.jpg)
![ELISA: Rat Nox4 ELISA Kit (Colorimetric) [NBP2-76792] - Samples were spiked with high concentrations of Rat Nox4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76792_rat-nox4-elisa-kit-colorimetric-13220241620486.jpg "ELISA: Rat Nox4 ELISA Kit (Colorimetric) [NBP2-76792] - Samples were spiked with high concentrations of Rat Nox4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Nox4 ELISA Kit (Colorimetric) [NBP2-76792] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76792_rat-nox4-elisa-kit-colorimetric-4320249192833.jpg)
![Western Blot: iNOS Antibody (3L0U6) [NBP3-16273] - Western blot analysis of lysates from RAW 264.7 cells using iNOS Rabbit mAb at 1:1000 dilution incubated overnight at 4C. Raw264.7 cells were treated by LPS (1 ug/ml) at 37C for 8 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 30 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-16273_rabbit-inos-mab-3l0u6-5220251265374.jpg "Western Blot: iNOS Antibody (3L0U6) [NBP3-16273] - Western blot analysis of lysates from RAW 264.7 cells using iNOS Rabbit mAb at 1:1000 dilution incubated overnight at 4C. Raw264.7 cells were treated by LPS (1 ug/ml) at 37C for 8 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 30 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/ Immunofluorescence: iNOS Antibody (3L0U6) [NBP3-16273] - Confocal imaging of RAW 264.7 cells (treated with LPS) and RAW 264.7 cells(untreated) using iNOS Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16273_rabbit-inos-mab-3l0u6-52202511392210.jpg)
![Western Blot: Dopamine beta Hydroxylase Antibody [NB300-112] - Western blot of human adrenal medulla. The antibody is specific for the ~75 kDa DBH protein.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Western-Blot-NB300-112-img0002.jpg "Western Blot: Dopamine beta Hydroxylase Antibody [NB300-112] - Western blot of human adrenal medulla. The antibody is specific for the ~75 kDa DBH protein.")
![Western Blot: Dopamine beta Hydroxylase Antibody [NB300-112] - Human adrenal medulla lysate showing specific immunolabeling of the ~75k DBH protein.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Western-Blot-NB300-112-img0001.jpg)
![Western Blot: iNOS Antibody (SR1134) [NBP3-21703] - Western blot analysis using NBP3-21703 in Human fetal brain lysate.](http://images.novusbio.com/fullsize/nbp3-21703_rabbit-inos-mab-sr1134-6720239533780.jpg "Western Blot: iNOS Antibody (SR1134) [NBP3-21703] - Western blot analysis using NBP3-21703 in Human fetal brain lysate.")
![Immunocytochemistry/Immunofluorescence: iNOS Antibody (SR1134) [NBP3-21703] - Immunofluorescent analysis of Raw264.7 cells treated with LPS, using NBP3-21703.](http://images.novusbio.com/fullsize/nbp3-21703_rabbit-inos-mab-sr1134-113202515145464.jpg)