| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55439-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92396-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-05407-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-93347-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89374-50ul | |||
![Western Blot: Adenylate Cyclase 2 Antibody [NBP3-12215] - 1:1000 antibody dilution in Diluobuffer.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-2-Antibody-Western-Blot-NBP3-12215-img0002.jpg "Western Blot: Adenylate Cyclase 2 Antibody [NBP3-12215] - 1:1000 antibody dilution in Diluobuffer.")
![Immunohistochemistry-Paraffin: Adenylate Cyclase 2 Antibody [NBP3-12215] - 1:50 dilution in IHC blocking buffer. DAB(brown) staining and hematoxylin QS (blue) counterstain. 40X magnification. FFPE section.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-2-Antibody-Immunohistochemistry-Paraffin-NBP3-12215-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Acetyl-CoA Carboxylase alpha/ACACA Antibody [NBP2-55439] - Staining of human cell line U-251 MG shows localization to nucleoli fibrillar center, cytosol & actin filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Acetyl-CoA-Carboxylase-alpha-ACACA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55439-img0001.jpg "Immunocytochemistry/Immunofluorescence: Acetyl-CoA Carboxylase alpha/ACACA Antibody [NBP2-55439] - Staining of human cell line U-251 MG shows localization to nucleoli fibrillar center, cytosol & actin filaments. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Acetyl-CoA Carboxylase alpha/ACACA Antibody [NBP2-55439] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/antibody/nbp2-55439_rabbit-polyclonal-acetyl-coa-carboxylase-alpha-acaca-antibody-western-blot-28102024123133..png)
![Western Blot: Cysteinyl Leukotriene R1/CysLTR1 Antibody [NBP2-92396] - Analysis of extracts of various cell lines, using Cysteinyl Leukotriene R1/CysLTR1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.](http://images.novusbio.com/fullsize/Cysteinyl-Leukotriene-R1-CysLTR1-Antibody-Western-Blot-NBP2-92396-img0001.jpg "Western Blot: Cysteinyl Leukotriene R1/CysLTR1 Antibody [NBP2-92396] - Analysis of extracts of various cell lines, using Cysteinyl Leukotriene R1/CysLTR1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.")
![Western Blot: PTRF Antibody [NB100-60635] - Detection of Human and Mouse PTRF on HeLa whole cell lysate using NB100-60635. PTRF was also immunoprecipitated by rabbit anti-PTRF antibodies NB100-60633 and NB100-60634.](http://images.novusbio.com/fullsize/PTRF-Antibody-Western-Blot-NB100-60635-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PTRF Antibody [NB100-60635] - Detection of Human and Mouse PTRF on HeLa whole cell lysate using NB100-60635. PTRF was also immunoprecipitated by rabbit anti-PTRF antibodies NB100-60633 and NB100-60634.")
![Western Blot: Adenylate Cyclase 2 Antibody [NBP1-33659] - Sample (30 ug of whole cell lysate) A: IMR32 7.5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-2-Antibody-Western-Blot-NBP1-33659-img0003.jpg "Western Blot: Adenylate Cyclase 2 Antibody [NBP1-33659] - Sample (30 ug of whole cell lysate) A: IMR32 7.5% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Adenylate Cyclase 2 Antibody [NBP1-33659] - Paraffin-embedded OVCA, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-33659-img0002.jpg)
![Western Blot: PKM2 Antibody (1B10) [NBP2-42686] - Fig 1: The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HeLa cell lysate Lane 2 : Jurkat cell lysate Lane 3 : MCF7 cell lysate Lane 4 : A549 cell lysate Lane 5 : 293T cell lysate Fig 2: The Recombinant human PKM2 (50ng) protein was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/PKM2-Antibody-1B10-Western-Blot-NBP2-42686-img0003.jpg "Western Blot: PKM2 Antibody (1B10) [NBP2-42686] - Fig 1: The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HeLa cell lysate Lane 2 : Jurkat cell lysate Lane 3 : MCF7 cell lysate Lane 4 : A549 cell lysate Lane 5 : 293T cell lysate Fig 2: The Recombinant human PKM2 (50ng) protein was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Immunocytochemistry/Immunofluorescence: PKM2 Antibody (1B10) [NBP2-42686] - Analysis of PKM2 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PKM2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/PKM2-Antibody-1B10-Immunocytochemistry-Immunofluorescence-NBP2-42686-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: PKM2 Antibody (1B10) [NBP2-42686] - Analysis of PKM2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PKM2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/PKM2-Antibody-1B10-Immunocytochemistry-Immunofluorescence-NBP2-42686-img0004.jpg)
![Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [ac Ser79] Antibody [NBP3-05407] - Western blot analysis of extracts of 293T cells, using Acetyl-CoA Carboxylase alpha/ACACA antibody (NBP3-05407) at 1:1000 dilution.293T cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Acetyl-CoA-Carboxylase-alpha-ACACA-[ac-Ser79]-Antibody-Western-Blot-NBP3-05407-img0001.jpg "Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [ac Ser79] Antibody [NBP3-05407] - Western blot analysis of extracts of 293T cells, using Acetyl-CoA Carboxylase alpha/ACACA antibody (NBP3-05407) at 1:1000 dilution.293T cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody - Azide and BSA Free [Acetyl-CoA Carboxylase alpha/ACACA] - Western blot analysis of lysates from RAW264.7 cells, using Acetyl-CoA Carboxylase alpha/ACACA Rabbit pAb at 1:700 dilution. RAW264. 7 cells were treated by lambda-PP mixed solution (1ul) at 30C for 30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-05407_rabbit-polyclonal-acetyl-coa-carboxylase-alpha-acaca-p-ser79-antibody-5220251639637.jpg)
![Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody - Azide and BSA Free [Acetyl-CoA Carboxylase alpha/ACACA] - Western blot analysis of lysates from A-431 cells, using Acetyl-CoA Carboxylase alpha/ACACA Rabbit pAb at 1:700 dilution. A-431 cells were treated by lambda-PP mixed solution (1ul) at 30C for 30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-05407_rabbit-polyclonal-acetyl-coa-carboxylase-alpha-acaca-p-ser79-antibody-5220251626462.jpg)
![Western Blot: PLC-beta 3 Antibody [NBP2-93347] - Analysis of extracts of various cell lines, using PLC-beta 3 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Western-Blot-NBP2-93347-img0001.jpg "Western Blot: PLC-beta 3 Antibody [NBP2-93347] - Analysis of extracts of various cell lines, using PLC-beta 3 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.")
![Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-93347] - Immunohistochemistry of paraffin-embedded rat brain using PLC-beta 3 Rabbit pAb (NBP2-93347) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-93347-img0002.jpg)
![Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody (201) [NBP2-89374] - Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:69 kDa Observed band size:69 kDa](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-201-Western-Blot-NBP2-89374-img0002.jpg "Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody (201) [NBP2-89374] - Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:69 kDa Observed band size:69 kDa")
![Immunoprecipitation: Leukotriene A4 Hydrolase/LTA4H Antibody (201) [NBP2-89374] - Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg 293T Whole Cell Lysate0.5 uL anti-LTA4H rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-LTA4H rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 110 kDa Observed band size: 110 kDa](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-201-Immunoprecipitation-NBP2-89374-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Leukotriene A4 Hydrolase/LTA4H Antibody (201) [NBP2-89374] - Staining of Human LTA4H in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89374 (1:300) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89374_rabbit-monoclonal-leukotriene-a4-hydrolase-lta4h-antibody-201-235202512205518.png)
![Western Blot: TBC1D4 [p Thr642] Antibody (JE60-97) [NBP3-32749] - Western blot analysis of TBC1D4 on different lysates with Rabbit anti-TBC1D4 antibody (NBP3-32749) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: NIH/3T3 starve overnight then treated with 100nM Calyculin A for 50 minutes add 100ng/mL insulin for 20 minutes cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 147 kDa Observed band size: 160 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32749) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32749_rabbit-tbc1d4-p-thr642-mab-je60-97-278202415212425.jpg "Western Blot: TBC1D4 [p Thr642] Antibody (JE60-97) [NBP3-32749] - Western blot analysis of TBC1D4 on different lysates with Rabbit anti-TBC1D4 antibody (NBP3-32749) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: NIH/3T3 starve overnight then treated with 100nM Calyculin A for 50 minutes add 100ng/mL insulin for 20 minutes cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 147 kDa Observed band size: 160 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32749) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: TBC1D4 [p Thr642] Antibody (JE60-97) [NBP3-32749] - Immunocytochemistry analysis of NIH/3T3 cells starved overnight then treated with or without 100nM Calyculin A for 50 minutes add 100ng/mL insulin for 20 minutes labeling TBC1D4 with Rabbit anti-TBC1D4 antibody (NBP3-32749) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TBC1D4 antibody (NBP3-32749) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32749_rabbit-tbc1d4-p-thr642-mab-je60-97-29202410484887.jpg)