| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93781-0.02ml | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | DDX0321A488-100 | |||
| Alexa Fluor 546 | DDX0321A546-100 | |||
| Alexa Fluor 647 | DDX0321A647-100 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55361-25ul | |||
![Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody [NB300-176] - Western blot of human dorsal Raphe nucleus showing specifc immunolabeling of the ~55k tryptophan hydroxylase protein.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-Western-Blot-NB300-176-img0002.jpg "Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody [NB300-176] - Western blot of human dorsal Raphe nucleus showing specifc immunolabeling of the ~55k tryptophan hydroxylase protein.")
![Western Blot: iNOS Antibody (PSH03-69) [NBP3-32473] - Western blot analysis of iNOS on different lysates with Rabbit anti-iNOS antibody (NBP3-32473) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 1ug/mL LPS for 24 hours cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 131 kDa Observed band size: 131 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32473) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32473_rabbit-inos-mab-psh03-69-278202415295526.jpg "Western Blot: iNOS Antibody (PSH03-69) [NBP3-32473] - Western blot analysis of iNOS on different lysates with Rabbit anti-iNOS antibody (NBP3-32473) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 1ug/mL LPS for 24 hours cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 131 kDa Observed band size: 131 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32473) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: iNOS Antibody (PSH03-69) [NBP3-32473] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-iNOS antibody (NBP3-32473) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32473) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32473_rabbit-inos-mab-psh03-69-298202414304151.jpg)
![Immunocytochemistry/ Immunofluorescence: iNOS Antibody (PSH03-69) [NBP3-32473] - Immunocytochemistry analysis of RAW264.7 cells treated with or without 1μg/mL LPS for 6 hours labeling iNOS with Rabbit anti-iNOS antibody (NBP3-32473) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-iNOS antibody (NBP3-32473) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32473_rabbit-inos-mab-psh03-69-29202410334018.jpg)
![Western Blot: Cytochrome P450 4A Antibody [NBP1-31427] - Sample(30 ug of whole cell lysate) A:A431 B:H1299 7.5% SDS PAGE, antibody diluted at 1:100.](http://images.novusbio.com/fullsize/Cytochrome-P450-4A-Antibody-Western-Blot-NBP1-31427-img0005.jpg "Western Blot: Cytochrome P450 4A Antibody [NBP1-31427] - Sample(30 ug of whole cell lysate) A:A431 B:H1299 7.5% SDS PAGE, antibody diluted at 1:100.")
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 4A Antibody [NBP1-31427] - Analysis of HeLa, using NBP1-31427 at 1:200 dilution.](http://images.novusbio.com/fullsize/Cytochrome-P450-4A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31427-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 4A Antibody [NBP1-31427] - Paraffin-embedded mixed OVCA, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Cytochrome-P450-4A-Antibody-Immunohistochemistry-Paraffin-NBP1-31427-img0004.jpg)
![Immunohistochemistry-Paraffin: P2Y2 Antibody [NBP3-12229] - 1:100 dilution in IHC blocking buffer. DAB (brown) staining and hematoxyllin QS (blue) counterstrain. 20x magnification on leica DM4000. FFPE section](http://images.novusbio.com/fullsize/P2Y2-Antibody-Immunohistochemistry-Paraffin-NBP3-12229-img0001.jpg "Immunohistochemistry-Paraffin: P2Y2 Antibody [NBP3-12229] - 1:100 dilution in IHC blocking buffer. DAB (brown) staining and hematoxyllin QS (blue) counterstrain. 20x magnification on leica DM4000. FFPE section")
![Western Blot: Pyruvate Carboxylase Antibody - BSA Free [NBP2-93781] - Western blot analysis of various lysates, using Pyruvate Carboxylase Rabbit pAb (A6301) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp2-93781_rabbit-polyclonal-pyruvate-carboxylase-antibody-5220251931355.jpg "Western Blot: Pyruvate Carboxylase Antibody - BSA Free [NBP2-93781] - Western blot analysis of various lysates, using Pyruvate Carboxylase Rabbit pAb (A6301) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3s.")
![Immunoprecipitation: Pyruvate Carboxylase Antibody [NBP2-93781] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Pyruvate Carboxylase antibody (NBP2-93781). Western blot was performed from the immunoprecipitate using Pyruvate Carboxylase antibody (NBP2-93781) at a dilution of 1:1000.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunoprecipitation-NBP2-93781-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Pyruvate Carboxylase Antibody [NBP2-93781] - Analysis of U2OS cells using Pyruvate Carboxylase . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93781-img0001.jpg)
![Flow Cytometry: CD39/ENTPD1 Antibody (AC2.5) [DDX0321P-100]](http://images.novusbio.com/fullsize/CD39-ENTPD1-Antibody-AC2-5-Flow-Cytometry-DDX0321P-100-img0001.jpg "Flow Cytometry: CD39/ENTPD1 Antibody (AC2.5) [DDX0321P-100]")
![Western Blot: Catalase Antibody (S05-5D9) [NBP3-14915] - Western blot detection of Catalase in K562, Hela cell lysates using Catalase Rabbit mAb (1:1000 diluted). Predicted band size: 60kDa. Observed band size: 60kDa.](http://images.novusbio.com/fullsize/Catalase-Antibody-S05-5D9-Western-Blot-NBP3-14915-img0001.jpg "Western Blot: Catalase Antibody (S05-5D9) [NBP3-14915] - Western blot detection of Catalase in K562, Hela cell lysates using Catalase Rabbit mAb (1:1000 diluted). Predicted band size: 60kDa. Observed band size: 60kDa.")
![Immunocytochemistry/Immunofluorescence: Catalase Antibody (S05-5D9) [NBP3-14915] - Immunofluorescence of Catalase (green) in Hela cells using Catalase Rabbit mAb at dilution 1/200, and DAPI (blue)](http://images.novusbio.com/fullsize/Catalase-Antibody-S05-5D9-Immunocytochemistry-Immunofluorescence-NBP3-14915-img0002.jpg)
![Immunohistochemistry-Paraffin: Catalase Antibody (S05-5D9) [NBP3-14915] - Immunohistochemistry of Catalase in paraffin-embedded Human breast cancer tissue using Catalase Rabbit mAb at dilution 1/1](http://images.novusbio.com/fullsize/Catalase-Antibody-S05-5D9-Immunohistochemistry-Paraffin-NBP3-14915-img0003.jpg)
![Western Blot: Pyruvate Carboxylase Antibody [NBP2-55361] - Analysis using Anti-PC antibody NBP2-55361 (A) shows similar pattern to independent antibody NBP2-33407 (B).](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Western-Blot-NBP2-55361-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Pyruvate Carboxylase Antibody [NBP2-55361] - Analysis using Anti-PC antibody NBP2-55361 (A) shows similar pattern to independent antibody NBP2-33407 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Pyruvate Carboxylase Antibody [NBP2-55361] - Staining of human cell line MCF7 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55361-img0001.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Pyruvate Carboxylase Antibody [NBP2-55361] - Analysis in human cell lines HeLa and U-251M. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Western-Blot-NBP2-55361-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free [NBP3-25442] - Dopamine beta Hydroxylase antibody [HL1163] detects Dopamine beta Hydroxylase protein at cytoplasm by immunofluorescent analysis. Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dopamine beta Hydroxylase stained by Dopamine beta Hydroxylase antibody [HL1163] (NBP3-25442) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-25442_rabbit-dopamine-beta-hydroxylase-mab-hl1163-azide-bsa-free-2120249425432.jpg "Immunocytochemistry/Immunofluorescence: Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free [NBP3-25442] - Dopamine beta Hydroxylase antibody [HL1163] detects Dopamine beta Hydroxylase protein at cytoplasm by immunofluorescent analysis. Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dopamine beta Hydroxylase stained by Dopamine beta Hydroxylase antibody [HL1163] (NBP3-25442) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free [NBP3-25442] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine beta Hydroxylase antibody [HL1163] (NBP3-25442) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25442_rabbit-dopamine-beta-hydroxylase-mab-hl1163-azide-bsa-free-2120249393558.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free [NBP3-25442] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine beta Hydroxylase antibody [HL1163] (NBP3-25442) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25442_rabbit-dopamine-beta-hydroxylase-mab-hl1163-azide-bsa-free-2120249371522.jpg)
![Western Blot: CYP11B2 Antibody [NBP1-56518] - Transfected HEK293-CYP11B2, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/CYP11B2-Antibody-Western-Blot-NBP1-56518-img0003.jpg "Western Blot: CYP11B2 Antibody [NBP1-56518] - Transfected HEK293-CYP11B2, Antibody Titration: 0.2-1 ug/ml")
![Western Blot: CYP11B2 Antibody [NBP1-56518] - Sample type: 1. HEK293TN-GFP-hcyp11B1 (75ug) 2. HEK293TN-GFP-hcyp11B2 (75ug) Primary Dilution: 1:100 Secondary Antibody: mouse anti-Rabbit HRP Secondary Dilution: 1:10,000 Film Exposed for: 15 minutes](http://images.novusbio.com/fullsize/CYP11B2-Antibody-Western-Blot-NBP1-56518-img0002.jpg)