| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54776-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05061-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22634-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21931-50ul | |||
![Western Blot: Snail Antibody (HL2303) - Azide and BSA Free [NBP3-25689] - Untreated (-) and treated (+) HeLa whole cell extract (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody [HL2303] (NBP3-25689) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25689_rabbit-snail-mab-hl2303-azide-and-bsa-free-2120249374962.jpg "<b>Biological Strategies Validation. </b>Western Blot: Snail Antibody (HL2303) - Azide and BSA Free [NBP3-25689] - Untreated (-) and treated (+) HeLa whole cell extract (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody [HL2303] (NBP3-25689) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Snail Antibody (HL2303) - Azide and BSA Free [NBP3-25689] - Wild-type (WT) and SNAI1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody [HL2303] (NBP3-25689) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25689_rabbit-snail-mab-hl2303-azide-and-bsa-free-212024941332.jpg)
![Immunocytochemistry/Immunofluorescence: Snail Antibody (HL2303) - Azide and BSA Free [NBP3-25689] - SNAI1 antibody [HL2303] detects SNAI1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SNAI1 stained by SNAI1 antibody [HL2303] (NBP3-25689) diluted at 1:1000. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25689_rabbit-snail-mab-hl2303-azide-and-bsa-free-2120249425477.jpg)
![Western Blot: Snail [p Ser246] Antibody [NBP2-54776] - analysis of extracts from HT29 cells using SNAI1 (Phospho-Ser246) Antibody. The lane on the right is treated with the antigen-specific peptide.](http://images.novusbio.com/fullsize/Snail-[p-Ser246]-Antibody-Western-Blot-NBP2-54776-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Snail [p Ser246] Antibody [NBP2-54776] - analysis of extracts from HT29 cells using SNAI1 (Phospho-Ser246) Antibody. The lane on the right is treated with the antigen-specific peptide.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Snail [p Ser246] Antibody - BSA Free [NBP2-54776] - Immunofluorescence staining of methanol-fixed HuvEc cells using Snail (Phospho-Ser246) Antibody.](http://images.novusbio.com/fullsize/nbp2-54776_rabbit-polyclonal-snail-p-ser246-antibody-2711202419541572.jpg)
![Western Blot: MID1 Antibody [NBP3-05061] - analysis of extracts of HeLa cells, using MID1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/MID1-Antibody-Western-Blot-NBP3-05061-img0003.jpg "Western Blot: MID1 Antibody [NBP3-05061] - analysis of extracts of HeLa cells, using MID1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunocytochemistry/Immunofluorescence: MID1 Antibody [NBP3-05061] - Analysis of MCF-7 cells using MID1 antibody . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/MID1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05061-img0001.jpg)
![Western Blot: Snail Antibody [NB100-53787] - Staining of Mouse (A) and Rat (B) Kidney lysate (35 ug protein in RIPA buffer) with antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Snail-Antibody-Western-Blot-NB100-53787-img0001.jpg "Western Blot: Snail Antibody [NB100-53787] - Staining of Mouse (A) and Rat (B) Kidney lysate (35 ug protein in RIPA buffer) with antibody at 0.1 ug/mL. Detected by chemiluminescence.")
![Flow Cytometry: Abhd5 Antibody (JE60-62) [NBP3-31989] - Flow cytometric analysis of NIH/3T3 cells labeling Abhd5. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-31989, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-31989_rabbit-abhd5-mab-je60-62-29202416453253.jpg "Flow Cytometry: Abhd5 Antibody (JE60-62) [NBP3-31989] - Flow cytometric analysis of NIH/3T3 cells labeling Abhd5. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-31989, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).")
![Western Blot: Abhd5 Antibody (JE60-62) [NBP3-31989] - Western blot analysis of Abhd5 on different lysates with Rabbit anti-Abhd5 antibody (NBP3-31989) at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate (20 ug/Lane) Lane 2: Mouse pancreas tissue lysate (40 ug/Lane) Lane 3: Mouse kidney tissue lysate (40 ug/Lane) Predicted band size: 39 kDa Observed band size: 43 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-31989) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-31989_rabbit-abhd5-mab-je60-62-3920248453521.jpg)
![Western Blot: dUTPase Antibody (SR1280) [NBP3-22634] - Western blot analysis using NBP3-22634 in Ramos cell lysate.](http://images.novusbio.com/fullsize/nbp3-22634_rabbit-dutpase-mab-sr1280-5720231564713.jpg "Western Blot: dUTPase Antibody (SR1280) [NBP3-22634] - Western blot analysis using NBP3-22634 in Ramos cell lysate.")
![Western Blot: Abhd5 Antibody (SR1756) [NBP3-21931] - Western blot analysis using NBP3-21931 in HepG2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21931_rabbit-abhd5-mab-sr1756-57202317215112.jpg "Western Blot: Abhd5 Antibody (SR1756) [NBP3-21931] - Western blot analysis using NBP3-21931 in HepG2 cell lysate.")