| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26565-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54501-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26636-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47999-0.1mg | |||
| IHC-Prediluted | NBP2-48230 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26590-50ul | |||
![Western Blot: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Western blot analysis of 5'-Nucleotidase/CD73 on different lysates with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/2,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: U-87 MG cell lysate (no heat) Lysates/proteins at 30 ug/Lane. Predicted band size: 63 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32166) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32166_mouse-5-nucleotidase-cd73-mab-a6g2-r-278202416285219.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Western blot analysis of 5'-Nucleotidase/CD73 on different lysates with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/2,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: U-87 MG cell lysate (no heat) Lysates/proteins at 30 ug/Lane. Predicted band size: 63 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32166) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Immunohistochemical analysis of paraffin-embedded NCI-H441 xenograft tissue with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32166) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32166_mouse-5-nucleotidase-cd73-mab-a6g2-r-298202414304184.jpg)
![Immunocytochemistry/ Immunofluorescence: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Immunocytochemistry analysis of U-87 MG cells labeling 5'-Nucleotidase/CD73 with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32166_mouse-5-nucleotidase-cd73-mab-a6g2-r-2920241192315.jpg)
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (JE58-27) [NBP3-32787] - Western blot analysis of Pyruvate Dehydrogenase E1-alpha subunit on different lysates with Rabbit anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-32787) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: HeLa cell lysate (20 ug/Lane) Lane 5: HEK-293 cell lysate (20 ug/Lane) Lane 6: Human kidney tissue lysate (40 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 39 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32787) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32787_rabbit-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser293-mab-288202412442923.jpg "Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (JE58-27) [NBP3-32787] - Western blot analysis of Pyruvate Dehydrogenase E1-alpha subunit on different lysates with Rabbit anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-32787) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: HeLa cell lysate (20 ug/Lane) Lane 5: HEK-293 cell lysate (20 ug/Lane) Lane 6: Human kidney tissue lysate (40 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 39 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32787) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (JE58-27) [NBP3-32787] - Immunohistochemical analysis of paraffin-embedded human renal clear cell carcinoma tissue with Rabbit anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-32787) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32787) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32787_rabbit-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser293-mab-298202414115418.jpg)
![Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (JE58-27) [NBP3-32787] - Immunocytochemistry analysis of Jurkat cells treated with or without lambda pp labeling Pyruvate Dehydrogenase E1-alpha subunit with Rabbit anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-32787) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-32787) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32787_rabbit-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser293-mab-2920241114815.jpg)
![Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) - Azide and BSA Free [NBP2-54501] - Western Blot analysis of Kidney tissue lysate using alpha-Methylacyl-CoA Racemase/AMACR antibody (AMACR/1723).](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-AMACR-1723-Azide-and-BSA-Free-Western-Blot-NBP2-54501-img0004.jpg "Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) - Azide and BSA Free [NBP2-54501] - Western Blot analysis of Kidney tissue lysate using alpha-Methylacyl-CoA Racemase/AMACR antibody (AMACR/1723).")
![Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) - Azide and BSA Free [NBP2-54501] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma Stained with AMACR / p504S Monoclonal Antibody (AMACR/1723)](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-AMACR-1723-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54501-img0001.jpg)
![Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) - Azide and BSA Free [NBP2-54501] - Western Blot Analysis of PC3 cell lysate using alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723).](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-AMACR-1723-Azide-and-BSA-Free-Western-Blot-NBP2-54501-img0002.jpg)
![Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) - Azide and BSA Free [NBP2-54501] - Western Blot Analysis of Liver tissue lysate using alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723).](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-AMACR-1723-Azide-and-BSA-Free-Western-Blot-NBP2-54501-img0003.jpg)
![Immunohistochemistry: FTO Antibody (4G9) [NBP3-26565] - Image of FTO Antibody (4G9) diluted at 1:70 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26565_rabbit-fto-mab-4g9-27220248453212.jpg "Immunohistochemistry: FTO Antibody (4G9) [NBP3-26565] - Image of FTO Antibody (4G9) diluted at 1:70 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Immunohistochemistry: FTO Antibody (4G9) [NBP3-26565] - Image of FTO Antibody (4G9) diluted at 1:70 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26565_rabbit-fto-mab-4g9-27220248573310.jpg)
![Western Blot: FTO Antibody (4G9) [NBP3-26565] - Positive Western Blot detected in: 293T whole cell lysate, SH-SY5Y whole cell lysate, Raji whole cell lysate, Colo320 whole cell lysate, A549 whole cell lysate, PC3 whole cell lysate. All lanes: FTO Antibody at 0.7ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 59, 15, 7, 13 KDa Observed band size: 59 KDa](http://images.novusbio.com/fullsize/nbp3-26565_rabbit-fto-mab-4g9-282202411485835.jpg)
![Immunocytochemistry/Immunofluorescence: FTO Antibody (4G9) [NBP3-26565] - Staining of Hela cells with FTO Antibody (4G9) at 1:23, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26565_rabbit-fto-mab-4g9-282202412475115.jpg)
![Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) [NBP2-53390] - Western Blot analysis of Kidney tissue lysate using alpha-Methylacyl-CoA Racemase/AMACR antibody (AMACR/1723).](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-AMACR-1723-Western-Blot-NBP2-53390-img0004.jpg "Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) [NBP2-53390] - Western Blot analysis of Kidney tissue lysate using alpha-Methylacyl-CoA Racemase/AMACR antibody (AMACR/1723).")
![Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) [NBP2-53390] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma Stained with AMACR / p504S Monoclonal Antibody (AMACR/1723)](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-AMACR-1723-Immunohistochemistry-Paraffin-NBP2-53390-img0001.jpg)
![Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) [NBP2-53390] - Western Blot Analysis of PC3 cell lysate using alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723).](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-AMACR-1723-Western-Blot-NBP2-53390-img0002.jpg)
![Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) [NBP2-53390] - Western Blot Analysis of Liver tissue lysate using alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723).](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-AMACR-1723-Western-Blot-NBP2-53390-img0003.jpg)
![Immunohistochemistry: Aromatase Antibody (1H1) [NBP3-26636] - NBP3-26636 diluted at 1:300 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody](http://images.novusbio.com/fullsize/nbp3-26636_rabbit-aromatase-mab-1h1-2612025101148.jpg "Immunohistochemistry: Aromatase Antibody (1H1) [NBP3-26636] - NBP3-26636 diluted at 1:300 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody")
![Immunocytochemistry/Immunofluorescence: Aromatase Antibody (1H1) [NBP3-26636] - Staining of Hela cell with NBP3-26636 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26636_rabbit-aromatase-mab-1h1-2612025101147.jpg)
![Western Blot: Aromatase Antibody (1H1) [NBP3-26636] - Positive WB detected in: 293 whole cell lysate (20µg), K562 whole cell lysate (20µg), Hela whole cell lysate (20µg), MCF-7 whole cell lysate (20µg), PC-3 whole cell lysate (20µg)All lanes: CYP19A1 antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 58 kDaObserved band size: 58 kDaExposure time：120s](http://images.novusbio.com/fullsize/nbp3-26636_rabbit-aromatase-mab-1h1-2612025101143.jpg)
![Immunocytochemistry/Immunofluorescence: Aromatase Antibody (1H1) [NBP3-26636] - Staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26636_rabbit-aromatase-mab-1h1-26120251011416.jpg)
![Western Blot: PKLR Antibody [NBP2-20027] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKLR Antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PKLR-Antibody-Western-Blot-NBP2-20027-img0004.jpg "Western Blot: PKLR Antibody [NBP2-20027] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKLR Antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: PKLR Antibody [NBP2-20027] - Human breast cancer, using Pyruvate Kinase (liver/RBC) antibody at 1:250 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PKLR-Antibody-Immunohistochemistry-Paraffin-NBP2-20027-img0001.jpg)
![Western Blot: PKLR Antibody [NBP2-20027] - A. 20 ug Rat liver lysate/extract 7.5% SDS-PAGE Pyruvate Kinase (liver/RBC) antibody dilution: 1:10000](http://images.novusbio.com/fullsize/PKLR-Antibody-Western-Blot-NBP2-20027-img0003.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody (ARG1/1126) [NBP2-44966] - Western Blot Analysis of Liver tissue lysate using Arginase 1/ARG1/liver Arginase antibody (ARG1/1126).](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-ARG1-1126-Western-Blot-NBP2-44966-img0003.jpg "Western Blot: Arginase 1/ARG1/liver Arginase Antibody (ARG1/1126) [NBP2-44966] - Western Blot Analysis of Liver tissue lysate using Arginase 1/ARG1/liver Arginase antibody (ARG1/1126).")
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody (ARG1/1126) [NBP2-44966] - Human Hepatocellular Carcinoma stained with ARG1 Monoclonal Antibody (ARG1/1126).](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-ARG1-1126-Immunohistochemistry-Paraffin-NBP2-44966-img0001.jpg)
![Protein Array: Arginase 1/ARG1/liver Arginase Antibody (ARG1/1126) [NBP2-44966] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Arginase 1/ARG1/liver Arginase Antibody (ARG1/1126).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-ARG1-1126-Protein-Array-NBP2-44966-img0002.jpg)
![Immunohistochemistry: FADS1 Antibody (7A10) [NBP3-26590] - Image of FADS1 Antibody (7A10) diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26590_rabbit-fads1-mab-7a10-262202414471574.jpg "Immunohistochemistry: FADS1 Antibody (7A10) [NBP3-26590] - Image of FADS1 Antibody (7A10) diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: FADS1 Antibody (7A10) [NBP3-26590] - Positive Western Blot detected in: MCF-7 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate. All lanes: FADS1 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 52, 43 kDa Observed band size: 55 kDa](http://images.novusbio.com/fullsize/nbp3-26590_rabbit-fads1-mab-7a10-28220241152510.jpg)
![Immunocytochemistry/Immunofluorescence: FADS1 Antibody (7A10) [NBP3-26590] - Staining of HepG2 cell with FADS1 Antibody (7A10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 565-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26590_rabbit-fads1-mab-7a10-282202412475112.jpg)
![Flow Cytometry: FADS1 Antibody (7A10) [NBP3-26590] - Overlay Peak curve showing HepG2 cells stained with FADS1 Antibody (7A10) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26590_rabbit-fads1-mab-7a10-282202414183689.jpg)
![Western Blot: PDE8B Antibody [NB300-644] - Rat testis and heart membranes. Antibody (NB300-644) dilution 1:500. Molecular weight marker positions are marked on the left.](http://images.novusbio.com/fullsize/p_449af994eafbe.jpg "Western Blot: PDE8B Antibody [NB300-644] - Rat testis and heart membranes. Antibody (NB300-644) dilution 1:500. Molecular weight marker positions are marked on the left.")
![PDE8B Antibody [NB300-644] - Human ovarian medulla tissue. 1:2000 antibody dilution Image courtesy of Steven S. Petersen, MD, PHD Copenhagen University Hospital, Denmark.](http://images.novusbio.com/fullsize/PDE8B-Antibody-NB300-644-img0002.jpg)