| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35822-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37454-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37350-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59692-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37271-0.025ml | |||
![Western Blot: COX4 Antibody (9X9I0) [NBP3-15416] - Western blot analysis of extracts of various cell lines, using COX4 Rabbit mAb (NBP3-15416) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/COX4-Antibody-9X9I0-Western-Blot-NBP3-15416-img0002.jpg "Western Blot: COX4 Antibody (9X9I0) [NBP3-15416] - Western blot analysis of extracts of various cell lines, using COX4 Rabbit mAb (NBP3-15416) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: COX4 Antibody (9X9I0) [NBP3-15416] - Immunohistochemistry of paraffin-embedded human colon carcinoma using COX4 Rabbit mAb (NBP3-15416) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/COX4-Antibody-9X9I0-Immunohistochemistry-Paraffin-NBP3-15416-img0001.jpg)
![Immunohistochemistry: COX4 Antibody (3P8J2) [COX4] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using COX4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15416_rabbit-cox4-mab-3p8j2-5220251726031.jpg)
![Immunohistochemistry: COX4 Antibody (3P8J2) [COX4] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using COX4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15416_rabbit-cox4-mab-3p8j2-52202517223979.jpg)
![Immunohistochemistry: COX4 Antibody (3P8J2) [COX4] - Immunohistochemistry analysis of paraffin-embedded Human colon using COX4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15416_rabbit-cox4-mab-3p8j2-52202517284940.jpg)
![Immunohistochemistry: COX4 Antibody (3P8J2) [COX4] - Immunohistochemistry analysis of paraffin-embedded Human kidney using COX4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15416_rabbit-cox4-mab-3p8j2-52202517162399.jpg)
![Immunohistochemistry: COX4 Antibody (3P8J2) [COX4] - Immunohistochemistry analysis of paraffin-embedded Human tonsil using COX4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15416_rabbit-cox4-mab-3p8j2-5220251726025.jpg)
![Immunohistochemistry: PTRF Antibody [NBP3-35822] - Immunohistochemistry analysis of paraffin-embedded Human placenta using PTRF Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35822_rabbit-ptrf-pab-912202415152875.jpg "Immunohistochemistry: PTRF Antibody [NBP3-35822] - Immunohistochemistry analysis of paraffin-embedded Human placenta using PTRF Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunocytochemistry/ Immunofluorescence: PTRF Antibody [NBP3-35822] - Immunofluorescence analysis of C6 cells using PTRF Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35822_rabbit-ptrf-pab-912202415171113.jpg)
![Western Blot: PTRF Antibody [NBP3-35822] - Western blot analysis of various lysates using PTRF Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35822_rabbit-ptrf-pab-912202415175297.jpg)
![Immunocytochemistry/ Immunofluorescence: PTRF Antibody [NBP3-35822] - Immunofluorescence analysis of U-2 OS cells using PTRF Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35822_rabbit-ptrf-pab-912202414163015.jpg)
![Immunocytochemistry/ Immunofluorescence: PTRF Antibody [NBP3-35822] - Immunofluorescence analysis of L929 cells using PTRF Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35822_rabbit-ptrf-pab-912202415152835.jpg)
![Immunohistochemistry: PTRF Antibody [NBP3-35822] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using PTRF Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35822_rabbit-ptrf-pab-912202414123822.jpg)
![Western Blot: Aconitase 2 Antibody (0P3H3) [NBP3-16426] - Western blot analysis of extracts of various cell lines, using Aconitase 2 (ACO2) Rabbit mAb (NBP3-16426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-0P3H3-Western-Blot-NBP3-16426-img0005.jpg "Western Blot: Aconitase 2 Antibody (0P3H3) [NBP3-16426] - Western blot analysis of extracts of various cell lines, using Aconitase 2 (ACO2) Rabbit mAb (NBP3-16426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: Aconitase 2 Antibody (0P3H3) [NBP3-16426] - Western blot analysis of extracts of various cell lines, using Aconitase 2 (ACO2) Rabbit mAb (NBP3-16426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-0P3H3-Western-Blot-NBP3-16426-img0006.jpg)
![Immunohistochemistry: Aconitase 2 Antibody (0P3H3) [NBP3-16426] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Aconitase 2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16426_rabbit-aconitase-2-mab-0p3h3-5220251242556.jpg)
![Immunocytochemistry/ Immunofluorescence: Aconitase 2 Antibody (0P3H3) [NBP3-16426] - Immunofluorescence analysis of NIH-3T3 cells using Aconitase 2 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16426_rabbit-aconitase-2-mab-0p3h3-5220251242512.jpg)
![Immunohistochemistry: Aconitase 2 Antibody (0P3H3) [NBP3-16426] - Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using Aconitase 2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16426_rabbit-aconitase-2-mab-0p3h3-52202511383784.jpg)
![Immunohistochemistry: Aconitase 2 Antibody (0P3H3) [NBP3-16426] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Aconitase 2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16426_rabbit-aconitase-2-mab-0p3h3-52202511361218.jpg)
![Western Blot: SHP-2/PTPN11 Antibody (6D9) [NBP2-37454] - Analysis using PTPN11 mAb against human PTPN11 recombinant protein. (Expected MW is 33.4 kDa)](http://images.novusbio.com/fullsize/SHP-2-PTPN11-Antibody-6D9-Western-Blot-NBP2-37454-img0002.jpg "Western Blot: SHP-2/PTPN11 Antibody (6D9) [NBP2-37454] - Analysis using PTPN11 mAb against human PTPN11 recombinant protein. (Expected MW is 33.4 kDa)")
![Immunocytochemistry/Immunofluorescence: SHP-2/PTPN11 Antibody (6D9) [NBP2-37454] - Analysis of HeLa cells using PTPN11 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/SHP-2-PTPN11-Antibody-6D9-Immunocytochemistry-Immunofluorescence-NBP2-37454-img0003.jpg)
![Immunohistochemistry-Paraffin: SHP-2/PTPN11 Antibody (6D9) [NBP2-37454] - Analysis of breast cancer tissues using PTPN11 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/SHP-2-PTPN11-Antibody-6D9-Immunohistochemistry-NBP2-37454-img0005.jpg)
![Flow Cytometry: SHP-2/PTPN11 Antibody (6D9) [NBP2-37454] - An intracellular stain was performed on HeLa cells with SHP-2/PTPN11 Antibody (6D9) NBP2-37454F and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/SHP-2-PTPN11-Antibody-6D9-Flow-Cytometry-NBP2-37454-img0006.jpg)
![Flow Cytometry: SHP-2/PTPN11 Antibody (6D9) [NBP2-37454] - Analysis of HepG2 cells using PTPN11 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/SHP-2-PTPN11-Antibody-6D9-Flow-Cytometry-NBP2-37454-img0004.jpg)
![ELISA: SHP-2/PTPN11 Antibody (6D9) [NBP2-37454] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/SHP-2-PTPN11-Antibody-6D9-ELISA-NBP2-37454-img0001.jpg)
![Western Blot: MRP4/ABCC4 Antibody (2D2A9) [NBP2-37350] - Western blot analysis using ABCC4 mAb against HEK293 (1) and ABCC4 (AA: 631-692)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/MRP4-ABCC4-Antibody-2D2A9-Western-Blot-NBP2-37350-img0003.jpg "Western Blot: MRP4/ABCC4 Antibody (2D2A9) [NBP2-37350] - Western blot analysis using ABCC4 mAb against HEK293 (1) and ABCC4 (AA: 631-692)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: MRP4/ABCC4 Antibody (2D2A9) [NBP2-37350] - Analysis of cancer tissues using ABCC4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MRP4-ABCC4-Antibody-2D2A9-Immunohistochemistry-NBP2-37350-img0006.jpg)
![Flow Cytometry: MRP4/ABCC4 Antibody (2D2A9) [NBP2-37350] - An intracellular stain was performed on A549 cells with MRP4/ABCC4 Antibody (2D2A9) NBP2-37350AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/MRP4-ABCC4-Antibody-2D2A9-Flow-Cytometry-NBP2-37350-img0007.jpg)
![Western Blot: MRP4/ABCC4 Antibody (2D2A9) [NBP2-37350] - Western blot analysis using ABCC4 mAb against human ABCC4 recombinant protein. (Expected MW is 32.4 kDa)](http://images.novusbio.com/fullsize/MRP4-ABCC4-Antibody-2D2A9-Western-Blot-NBP2-37350-img0002.jpg)
![Immunohistochemistry-Paraffin: MRP4/ABCC4 Antibody (2D2A9) [NBP2-37350] - Analysis of endometrial cancer tissues using ABCC4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MRP4-ABCC4-Antibody-2D2A9-Immunohistochemistry-NBP2-37350-img0005.jpg)
![Flow Cytometry: MRP4/ABCC4 Antibody (2D2A9) [NBP2-37350] - Flow cytometric analysis of A549 cells using ABCC4 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/MRP4-ABCC4-Antibody-2D2A9-Flow-Cytometry-NBP2-37350-img0004.jpg)
![ELISA: MRP4/ABCC4 Antibody (2D2A9) [NBP2-37350] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/MRP4-ABCC4-Antibody-2D2A9-ELISA-NBP2-37350-img0001.jpg)
![Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Analysis using PDK1 mouse mAb against NIH/3T3 (1), Hela (2), Jurkat (3), HepG2 (4), PC-12 (5), and Cos7 (6) cell lysate.](http://images.novusbio.com/fullsize/PDK-1-Antibody-4A11-Western-Blot-NBP2-22171-img0004.jpg "Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Analysis using PDK1 mouse mAb against NIH/3T3 (1), Hela (2), Jurkat (3), HepG2 (4), PC-12 (5), and Cos7 (6) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Immunofluorescence analysis of HELA cells using PDK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/PDK-1-Antibody-4A11-Immunocytochemistry-Immunofluorescence-NBP2-22171-img0002.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Analysis of breast cancer tissues (left) and brain tissues (right) using PDK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PDK-1-Antibody-4A11-Immunohistochemistry-Paraffin-NBP2-22171-img0003.jpg)
![Flow Cytometry: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Analysis of Lovo cells using PDK1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/PDK-1-Antibody-4A11-Flow-Cytometry-NBP2-22171-img0001.jpg)
![Western Blot: GRP78/HSPA5 Antibody (3C5-1A4) [NBP2-59692] - Analysis of Human, Mouse, Rat NIH3T3, Rat Brain, and HEK-293 cell lysates showing detection of ~78 kDa GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4 . Lane 1: MW ladder. Lane 2: Mouse NIH3T3. Lane 3: Rat Brain. Lane 4: Human HEK-293. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~78 kDa.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-3C5-1A4-Western-Blot-NBP2-59692-img0002.jpg "Western Blot: GRP78/HSPA5 Antibody (3C5-1A4) [NBP2-59692] - Analysis of Human, Mouse, Rat NIH3T3, Rat Brain, and HEK-293 cell lysates showing detection of ~78 kDa GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4 . Lane 1: MW ladder. Lane 2: Mouse NIH3T3. Lane 3: Rat Brain. Lane 4: Human HEK-293. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~78 kDa.")
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody (3C5-1A4) [NBP2-59692] - Mouse fibroblast cell line (NIH 3T3). Fixed in 4% Formaldehyde for 15 min at RT. Primary antibody at 1:100 for 60 min at RT. Secondary antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstaied with DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-3C5-1A4-Immunocytochemistry-Immunofluorescence-NBP2-59692-img0003.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mouse mAb against A431 cell lysate.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Western-Blot-NBP2-37271-img0004.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mouse mAb against A431 cell lysate.")
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis of esophageal cancer tissues using NT5E mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Immunohistochemistry-NBP2-37271-img0007.jpg)
![Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis of A431 cells using NT5E mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Flow-Cytometry-NBP2-37271-img0005.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mAb against human NT5E (AA: 30-250) recombinant protein. (Expected MW is 26.6 kDa)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Western-Blot-NBP2-37271-img0002.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mAb against HEK293 (1) and NT5E (AA: 30-250)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Western-Blot-NBP2-37271-img0003.jpg)
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis of endometrial cancer tissues using NT5E mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Immunohistochemistry-NBP2-37271-img0006.jpg)
![ELISA: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-ELISA-NBP2-37271-img0001.jpg)
![Western Blot: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Aconitase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Aconitase](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-OTI3G8-Azide-and-BSA-Free-Western-Blot-NBP2-70074-img0001.jpg "Western Blot: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Aconitase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Aconitase")
![Immunocytochemistry/Immunofluorescence: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Aconitase 2.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-OTI3G8-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70074-img0003.jpg)
![Immunohistochemistry: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - Staining of paraffin-embedded Kidney tissue using anti-Aconitase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70074-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - Staining of HepG2 cells using anti-Aconitase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-OTI3G8-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70074-img0002.jpg)
![Immunohistochemistry: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-Aconitase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70074-img0004.jpg)
![Immunohistochemistry: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - Staining of paraffin-embedded Ovary tissue using anti-Aconitase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70074-img0006.jpg)
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPHK1.](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-OTI1A6-Azide-and-BSA-Free-Western-Blot-NBP2-74332-img0002.jpg "Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPHK1.")
![Immunocytochemistry/Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-OTI1A6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74332-img0004.jpg)
![Immunohistochemistry: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-OTI1A6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74332-img0005.jpg)
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-OTI1A6-Azide-and-BSA-Free-Western-Blot-NBP2-74332-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY Sphingosine Kinase 1/SPHK1.](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-OTI1A6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74332-img0003.jpg)
![Immunohistochemistry: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-OTI1A6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74332-img0006.jpg)
![Western Blot: PDX-1/IPF1 Antibody (OTI3H3) - Azide and BSA Free [NBP2-73332] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDX1 .](http://images.novusbio.com/fullsize/PDX-1-IPF1-Antibody-OTI3H3-Azide-and-BSA-Free-Western-Blot-NBP2-73332-img0001.jpg "Western Blot: PDX-1/IPF1 Antibody (OTI3H3) - Azide and BSA Free [NBP2-73332] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDX1 .")
![Immunohistochemistry: PDX-1/IPF1 Antibody (OTI3H3) - Azide and BSA Free [NBP2-73332] - Staining of paraffin-embedded Human Kidney tissue using anti-PDX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,2) (1:150)](http://images.novusbio.com/fullsize/PDX-1-IPF1-Antibody-OTI3H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73332-img0006.jpg)
![Immunohistochemistry: PDX-1/IPF1 Antibody (OTI3H3) - Azide and BSA Free [NBP2-73332] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/PDX-1-IPF1-Antibody-OTI3H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73332-img0002.jpg)
![Immunohistochemistry: PDX-1/IPF1 Antibody (OTI3H3) - Azide and BSA Free [NBP2-73332] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/PDX-1-IPF1-Antibody-OTI3H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73332-img0003.jpg)
![Immunohistochemistry: PDX-1/IPF1 Antibody (OTI3H3) - Azide and BSA Free [NBP2-73332] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti PDX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)](http://images.novusbio.com/fullsize/PDX-1-IPF1-Antibody-OTI3H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73332-img0004.jpg)
![Immunohistochemistry: PDX-1/IPF1 Antibody (OTI3H3) - Azide and BSA Free [NBP2-73332] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PDX1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)](http://images.novusbio.com/fullsize/PDX-1-IPF1-Antibody-OTI3H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73332-img0005.jpg)
![Immunohistochemistry: PDX-1/IPF1 Antibody (OTI3H3) - Azide and BSA Free [NBP2-73332] - Staining paraffin-embedded Human liver tissue using anti-PDX1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)](http://images.novusbio.com/fullsize/PDX-1-IPF1-Antibody-OTI3H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73332-img0007.jpg)
![Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glut1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32385, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-298202414262648.jpg "Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glut1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32385, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Glut1 with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32385, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-298202414333665.jpg)
![Immunocytochemistry/ Immunofluorescence: Glut1 Antibody (SA0377) [NBP3-32385] - Immunocytochemistry analysis of HeLa cells labeling Glut1 with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/500 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Glucose Transporter GLUT1 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-29202410334092.jpg)
![Flow Cytometry: Glut1 Antibody (SA0377) [NBP3-32385] - Flow cytometric analysis of Jurkat cells labeling Glut1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32385, red) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (blue). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with an iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-29202416372147.jpg)
![Western Blot: Glut1 Antibody (SA0377) [NBP3-32385] - Western blot analysis of Glut1 on different lysates with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/50,000 dilution. Lane 1: HeLa cell lysate (no heat) Lane 2: HT-29 cell lysate (no heat) Lane 3: HepG2 cell lysate (no heat) Lane 4: NIH/3T3 cell lysate (no heat) Lane 5: L-929 cell lysate (no heat) Lane 6: Mouse brain tissue lysate (no heat) Lane 7: Rat brain tissue lysate (no heat) Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 45-60 kDa Exposure time: Lane 1-7 (left): 20 seconds; Lane 1-7 (right): 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/50,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-3920248453511.jpg)