| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35155-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95096-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06239-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89915-50ul | |||
![Western Blot: MAT2A Antibody (3A2) [NBP1-28605] - The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAT2A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : HeLa cell lysateLane 2. : Ramos cell lysateLane 3. : 293T cell lysateLane 4. : Jurkat cell lysateLane 5. : NIH-3T3 cell lysate](http://images.novusbio.com/fullsize/MAT2A-Antibody-3A2-Western-Blot-NBP1-28605-img0006.jpg "Western Blot: MAT2A Antibody (3A2) [NBP1-28605] - The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAT2A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : HeLa cell lysateLane 2. : Ramos cell lysateLane 3. : 293T cell lysateLane 4. : Jurkat cell lysateLane 5. : NIH-3T3 cell lysate")
![Immunocytochemistry/Immunofluorescence: MAT2A Antibody (3A2) [NBP1-28605] - Analysis of MAT2A in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human MAT2A antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/MAT2A-Antibody-3A2-Immunocytochemistry-Immunofluorescence-NBP1-28605-img0007.jpg)
![Immunohistochemistry-Paraffin: MAT2A Antibody (3A2) [NBP1-28605] - Human MAT2A (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).](http://images.novusbio.com/fullsize/MAT2A-Antibody-3A2-Immunohistochemistry-Paraffin-NBP1-28605-img0003.jpg)
![Western Blot: MAT2A Antibody (3A2) [NBP1-28605] - Cell lysates of HepG2 (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human MAT2A (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/MAT2A-Antibody-3A2-Western-Blot-NBP1-28605-img0004.jpg)
![Immunohistochemistry: SEC14L2 Antibody [NBP3-35155] - Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using SEC14L2 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35155_rabbit-sec14l2-pab-912202415133421.jpg "Immunohistochemistry: SEC14L2 Antibody [NBP3-35155] - Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using SEC14L2 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: SEC14L2 Antibody [NBP3-35155] - Immunohistochemistry analysis of paraffin-embedded Mouse pancreas using SEC14L2 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35155_rabbit-sec14l2-pab-912202415121312.jpg)
![Immunohistochemistry: SEC14L2 Antibody [NBP3-35155] - Immunohistochemistry analysis of paraffin-embedded Rat lung using SEC14L2 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35155_rabbit-sec14l2-pab-912202414201353.jpg)
![Immunocytochemistry/ Immunofluorescence: SEC14L2 Antibody [NBP3-35155] - Immunofluorescence analysis of U-2 OS cells using SEC14L2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35155_rabbit-sec14l2-pab-912202415175267.jpg)
![Western Blot: SEC14L2 Antibody [NBP3-35155] - Western blot analysis of various lysates using SEC14L2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35155_rabbit-sec14l2-pab-912202415152860.jpg)
![Western Blot: RPE65 Antibody [NBP2-95096] - Analysis of extracts of various cell lines, using RPE65 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.](http://images.novusbio.com/fullsize/RPE65-Antibody-Western-Blot-NBP2-95096-img0003.jpg "Western Blot: RPE65 Antibody [NBP2-95096] - Analysis of extracts of various cell lines, using RPE65 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.")
![Immunohistochemistry: RPE65 Antibody [NBP2-95096] - Analysis of Mouse eye cells using RPE65 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RPE65-Antibody-Immunohistochemistry-NBP2-95096-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: RPE65 Antibody - BSA Free [NBP2-95096] - Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded mouse retina using RPE65 Rabbit pAb (A9841) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95096_rabbit-polyclonal-rpe65-antibody-52202519302965.jpg)
![Immunocytochemistry/ Immunofluorescence: RPE65 Antibody - BSA Free [NBP2-95096] - Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded rat retina using RPE65 Rabbit pAb (A9841) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95096_rabbit-polyclonal-rpe65-antibody-5220251931373.jpg)
![Western Blot: RPE65 Antibody - BSA Free [NBP2-95096] - Western blot analysis of lysates from Mouse eye, using RPE65 Rabbit pAb (A9841) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp2-95096_rabbit-polyclonal-rpe65-antibody-52202519294553.jpg)
![Western Blot: NM23-H1 Antibody (4B2) [NBP1-47398] - Western blot analysis using NME1 mAb against NME1-hIgGFc transfected HEK293 cell lysate.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4B2-Western-Blot-NBP1-47398-img0011.jpg "Western Blot: NM23-H1 Antibody (4B2) [NBP1-47398] - Western blot analysis using NME1 mAb against NME1-hIgGFc transfected HEK293 cell lysate.")
![Immunocytochemistry/Immunofluorescence: NM23-H1 Antibody (4B2) [NBP1-47398] - Immunofluorescence analysis of Hela cells using NME1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4B2-Immunocytochemistry-Immunofluorescence-NBP1-47398-img0010.jpg)
![Immunohistochemistry-Paraffin: NM23-H1 Antibody (4B2) [NBP1-47398] - Immunohistochemical analysis of paraffin-embedded human Placenta tissues using anti-NME1 mouse mAb.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4B2-Immunohistochemistry-Paraffin-NBP1-47398-img0007.jpg)
![Flow Cytometry: NM23-H1 Antibody (4B2) [NBP1-47398] - Flow cytometric analysis of Jurkat cells using NME1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4B2-Flow-Cytometry-NBP1-47398-img0009.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free [NBP2-70204] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY liver Arginase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-liver A](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-OTI4E6-Azide-and-BSA-Free-Western-Blot-NBP2-70204-img0003.jpg "Western Blot: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free [NBP2-70204] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY liver Arginase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-liver A")
![Immunohistochemistry: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free [NBP2-70204] - Staining of paraffin-embedded Human liver tissue using anti-liver Arginase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-OTI4E6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70204-img0005.jpg)
![Flow Cytometry: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free [NBP2-70204] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-liver Arginase antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-OTI4E6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70204-img0004.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free [NBP2-70204] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using antiARG1 monoclonal antibody.(1:200)](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-OTI4E6-Azide-and-BSA-Free-Western-Blot-NBP2-70204-img0001.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free [NBP2-70204] - Analysis of extracts (35ug) from 9 different cell lines by using anti-liver Arginase monoclonal antibody.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-OTI4E6-Azide-and-BSA-Free-Western-Blot-NBP2-70204-img0002.jpg)
![Western Blot: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - HEK293T cells were transfected with the pCMV6-ENTRY control (left lane) or pCMV6-ENTRY CD38 (right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD38.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Azide-and-BSA-Free-Western-Blot-NBP2-70376-img0003.jpg "Western Blot: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - HEK293T cells were transfected with the pCMV6-ENTRY control (left lane) or pCMV6-ENTRY CD38 (right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD38.")
![Immunohistochemistry: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - Staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CD38 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70376-img0006.jpg)
![Western Blot: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - Analysis of cell extracts (35 ug) from 2 cell lines using anti-CD38 monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Azide-and-BSA-Free-Western-Blot-NBP2-70376-img0001.jpg)
![Western Blot: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - Analysis of cell extracts (35 ug) from 5 cell lines using anti-CD38 monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Azide-and-BSA-Free-Western-Blot-NBP2-70376-img0002.jpg)
![Immunohistochemistry: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CD38 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70376-img0004.jpg)
![Immunohistochemistry: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - Staining of paraffin-embedded Human liver tissue within the normal limits using anti-CD38 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70376-img0005.jpg)
![Immunohistochemistry: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - Staining of paraffin-embedded Human tonsil within the normal limits using anti-CD38 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70376-img0007.jpg)
![Western Blot: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FHL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FHL1.](http://images.novusbio.com/fullsize/FHL1-Antibody-OTI3G7-Azide-and-BSA-Free-Western-Blot-NBP2-70739-img0002.jpg "Western Blot: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FHL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FHL1.")
![Immunocytochemistry/Immunofluorescence: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FHL1.](http://images.novusbio.com/fullsize/FHL1-Antibody-OTI3G7-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70739-img0005.jpg)
![Flow Cytometry: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - Analysis of Jurkat cells, using anti-FHL1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/FHL1-Antibody-OTI3G7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70739-img0004.jpg)
![Western Blot: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - Analysis of extracts (35ug) from 9 different cell lines by using anti-FHL1 monoclonal antibody.](http://images.novusbio.com/fullsize/FHL1-Antibody-OTI3G7-Azide-and-BSA-Free-Western-Blot-NBP2-70739-img0001.jpg)
![Flow Cytometry: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - Analysis of Hela cells, using anti-FHL1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/FHL1-Antibody-OTI3G7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70739-img0003.jpg)
![Western Blot: CLPP Antibody (JE40-00) [NBP3-32195] - Western blot analysis of CLPP on different lysates with Rabbit anti-CLPP antibody (NBP3-32195) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: SW620 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: MCF7 cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Lane 9: Rat heart tissue lysate (39 ug/Lane) Predicted band size: 30 kDa Observed band size: 25 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32195) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32195_rabbit-clpp-mab-je40-00-278202416285288.jpg "Western Blot: CLPP Antibody (JE40-00) [NBP3-32195] - Western blot analysis of CLPP on different lysates with Rabbit anti-CLPP antibody (NBP3-32195) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: SW620 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: MCF7 cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Lane 9: Rat heart tissue lysate (39 ug/Lane) Predicted band size: 30 kDa Observed band size: 25 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32195) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CLPP Antibody (JE40-00) [NBP3-32195] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-CLPP antibody (NBP3-32195) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32195) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32195_rabbit-clpp-mab-je40-00-29820241492667.jpg)
![Immunocytochemistry/ Immunofluorescence: CLPP Antibody (JE40-00) [NBP3-32195] - Immunocytochemistry analysis of HeLa cells labeling CLPP with Rabbit anti-CLPP antibody (NBP3-32195) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CLPP antibody (NBP3-32195) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32195_rabbit-clpp-mab-je40-00-29202410334080.jpg)
![Flow Cytometry: CLPP Antibody (JE40-00) [NBP3-32195] - Flow cytometric analysis of HeLa cells labeling CLPP. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32195, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32195_rabbit-clpp-mab-je40-00-29202416372131.jpg)
![Western Blot: NM23-H1 Antibody (12A1-R) [NBP3-32656] - Western blot analysis of NM23-H1 on different lysates with Mouse anti-NM23-H1 antibody (NBP3-32656) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (20 ug/Lane) Lane 2: PC-3M cell lysate (20 ug/Lane) Lane 3: HeLa cell lysate (20 ug/Lane) Lane 4: HepG2 cell lysate (20 ug/Lane) Lane 5: A549 cell lysate (20 ug/Lane) Lane 4: NIH/3T3 cell lysate (20 ug/Lane) Lane 5: C6 cell lysate (20 ug/Lane) Lane 5: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 17 kDa Observed band size: 17/19 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32656) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32656_mouse-nm23-h1-mab-12a1-r-278202415212429.jpg "Western Blot: NM23-H1 Antibody (12A1-R) [NBP3-32656] - Western blot analysis of NM23-H1 on different lysates with Mouse anti-NM23-H1 antibody (NBP3-32656) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (20 ug/Lane) Lane 2: PC-3M cell lysate (20 ug/Lane) Lane 3: HeLa cell lysate (20 ug/Lane) Lane 4: HepG2 cell lysate (20 ug/Lane) Lane 5: A549 cell lysate (20 ug/Lane) Lane 4: NIH/3T3 cell lysate (20 ug/Lane) Lane 5: C6 cell lysate (20 ug/Lane) Lane 5: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 17 kDa Observed band size: 17/19 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32656) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NM23-H1 Antibody (12A1-R) [NBP3-32656] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-NM23-H1 antibody (NBP3-32656) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32656) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32656_mouse-nm23-h1-mab-12a1-r-298202414333679.jpg)
![Immunocytochemistry/ Immunofluorescence: NM23-H1 Antibody (12A1-R) [NBP3-32656] - Immunocytochemistry analysis of MCF7 cells labeling NM23-H1 with Mouse anti-NM23-H1 antibody (NBP3-32656) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-NM23-H1 antibody (NBP3-32656) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32656_mouse-nm23-h1-mab-12a1-r-29202410484818.jpg)
![Flow Cytometry: NM23-H1 Antibody (12A1-R) [NBP3-32656] - Flow cytometric analysis of MCF7 cells labeling NM23-H1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32656, 1μg/mL) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32656_mouse-nm23-h1-mab-12a1-r-29202416372150.jpg)
![Western Blot: CLPP Antibody [NBP3-06239] - Anti-CLPP rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLane B: 293T Whole Cell LysateLane C: K562. Whole Cell LysateLane D: A549 Whole Cell LysateLysatesroteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:30 kDaObserved band size:26 kDa](http://images.novusbio.com/fullsize/CLPP-Antibody-Western-Blot-NBP3-06239-img0003.jpg "Western Blot: CLPP Antibody [NBP3-06239] - Anti-CLPP rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLane B: 293T Whole Cell LysateLane C: K562. Whole Cell LysateLane D: A549 Whole Cell LysateLysatesroteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:30 kDaObserved band size:26 kDa")
![Immunoprecipitation: CLPP Antibody [NBP3-06239] -](http://images.novusbio.com/fullsize/CLPP-Antibody-Immunoprecipitation-NBP3-06239-img0002.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody - BSA Free [NBP3-06239] - Immunochemical staining of human CLPP in human liver with NBP3-06239 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-06239_rabbit-polyclonal-clpp-antibody-23520251433153.png)
![Immunohistochemistry-Paraffin: CLPP Antibody - BSA Free [NBP3-06239] - Immunochemical staining of human CLPP in human pancreas with NBP3-06239 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-06239_rabbit-polyclonal-clpp-antibody-2352025112932.png)
![Immunocytochemistry/Immunofluorescence: CLPP Antibody [NBP3-06239] - Immunofluorescence staining of CLPP in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-06239 antibody (dilution ratio 1:200) at 4 degrees celsius overnight. Then cells were stained with the Alexa Fluor (TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-06239_rabbit-polyclonal-clpp-antibody-23520251633504.png)
![Western Blot: PRAS40 Antibody (JE30-60) [NBP3-32850] - Western blot analysis of PRAS40 on different lysates with Rabbit anti-PRAS40 antibody (NBP3-32850) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: PC-12 cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 27 kDa Observed band size: 40 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32850) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32850_rabbit-pras40-mab-je30-60-288202412442995.jpg "Western Blot: PRAS40 Antibody (JE30-60) [NBP3-32850] - Western blot analysis of PRAS40 on different lysates with Rabbit anti-PRAS40 antibody (NBP3-32850) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: PC-12 cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 27 kDa Observed band size: 40 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32850) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PRAS40 Antibody (JE30-60) [NBP3-32850] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PRAS40 antibody (NBP3-32850) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32850) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32850_rabbit-pras40-mab-je30-60-298202413585526.jpg)
![Immunocytochemistry/ Immunofluorescence: PRAS40 Antibody (JE30-60) [NBP3-32850] - Immunocytochemistry analysis of HeLa cells labeling PRAS40 with Rabbit anti-PRAS40 antibody (NBP3-32850) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PRAS40 antibody (NBP3-32850) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32850_rabbit-pras40-mab-je30-60-2920241192362.jpg)
![Flow Cytometry: PRAS40 Antibody (JE30-60) [NBP3-32850] - Flow cytometric analysis of HeLa cells labeling PRAS40. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32850, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32850_rabbit-pras40-mab-je30-60-2920241637211.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody (034) [NBP2-89915] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDa Observed band size:40 kDa](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-034-Western-Blot-NBP2-89915-img0003.jpg "Western Blot: Arginase 1/ARG1/liver Arginase Antibody (034) [NBP2-89915] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDa Observed band size:40 kDa")
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody (034) [NBP2-89915] - Staining of human ARG1 in human kidney with rabbit monoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-034-Immunohistochemistry-Paraffin-NBP2-89915-img0002.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody (034) [NBP2-89915] - Staining of human ARG1 in human liver with rabbit monoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-034-Immunohistochemistry-Paraffin-NBP2-89915-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Arginase 1/ARG1/liver Arginase Antibody (034) [NBP2-89915] - Staining of ARG1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89915 antibody (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-89915_rabbit-monoclonal-arginase-1-arg1-liver-arginase-antibody-034-23520252135123.png)
![Flow Cytometry: Arginase 1/ARG1/liver Arginase Antibody (034) [NBP2-89915] - Analysis of Human ARG1 expression on HepG2 cells. The cells were treated, stained with NBP2-89915, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/nbp2-89915_rabbit-monoclonal-arginase-1-arg1-liver-arginase-antibody-034-235202511545610.png)