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![Western Blot: ACAT2 Antibody [NBP2-32198] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-ACAT2 antibody NBP2-32198 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Western-Blot-NBP2-32198-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: ACAT2 Antibody [NBP2-32198] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-ACAT2 antibody NBP2-32198 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP2-32198] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- ACAT2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-NBP2-32198-img0003.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP2-32198] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- ACAT2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-NBP2-32198-img0002.jpg)
![Western Blot: ACAT2 Antibody (55D5) [NBP2-42614] - The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Recombinant human ACAT2 protein](http://images.novusbio.com/fullsize/ACAT2-Antibody-55D5-Western-Blot-NBP2-42614-img0001.jpg "Western Blot: ACAT2 Antibody (55D5) [NBP2-42614] - The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Recombinant human ACAT2 protein")
![Immunocytochemistry/Immunofluorescence: ACAT2 Antibody (55D5) [NBP2-42614] - ICC/IF analysis of ACAT2 in HeLa cells. The cell was stained with ATGA0335 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).](http://images.novusbio.com/fullsize/ACAT2-Antibody-55D5-Immunocytochemistry-Immunofluorescence-NBP2-42614-img0004.jpg)
![ACAT2 Antibody (55D5) [NBP2-42614] - The cell lysates(5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: ACAT2 Transfected 293T cell lysate](http://images.novusbio.com/fullsize/ACAT2-Antibody-55D5-NBP2-42614-img0002.jpg)
![ACAT2 Antibody (55D5) [NBP2-42614] - The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HepG2 cell lysateLane 2.: Ramos cell lysateLane 3.: MCF7 cell lysateLane 4.: HL-60 cell lysateLane 5.: SW480 cell lysate](http://images.novusbio.com/fullsize/ACAT2-Antibody-55D5-NBP2-42614-img0003.jpg)
![ACAT2 Antibody (55D5) [NBP2-42614] - Analysis of ACAT2 in PC3 cells. The cells were stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/ACAT2-Antibody-55D5-NBP2-42614-img0005.jpg)
![Western Blot: SR-BI Antibody (185-1G2 (B467)) - Chimeric [NBP3-12073] - Human peripheral blood monocyte cell lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 185-1G2 (B467) (NBP3-12073) at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/SR-BI-Antibody-185-1G2-B467-Chimeric-Western-Blot-NBP3-12073-img0001.jpg "Western Blot: SR-BI Antibody (185-1G2 (B467)) - Chimeric [NBP3-12073] - Human peripheral blood monocyte cell lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 185-1G2 (B467) (NBP3-12073) at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry /Immunofluorescence: SR-BI Antibody (185-1G2 (B467)) - Chimeric - Azide and BSA Free [NBP3-12073] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on Shi-fix(TM) coverslips stained with NBP3-12073 at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12073, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-12073_rabbit-monoclonal-sr-bi-antibody-185-1g2-b467-chimeric-74202522141242.png)
![Western Blot: ACAT1 Antibody [NBP1-51994] - Analysis of Mouse (A) and Rat (B) kidney lysates, and Mouse (C) and Rat (D) Liver lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-51994-img0003.jpg "Western Blot: ACAT1 Antibody [NBP1-51994] - Analysis of Mouse (A) and Rat (B) kidney lysates, and Mouse (C) and Rat (D) Liver lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: ACAT1 Antibody [NBP1-51994] - (0.01ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-51994-img0002.jpg)
![Western Blot: ACAT1 Antibody (15E5) [NBP2-22565] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Hep3B cell lysate Lane 2. : 293T cell lysate Lane 3. : A431 cell lysate.](http://images.novusbio.com/fullsize/ACAT1-Antibody-15E5-Western-Blot-NBP2-22565-img0003.jpg "Western Blot: ACAT1 Antibody (15E5) [NBP2-22565] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Hep3B cell lysate Lane 2. : 293T cell lysate Lane 3. : A431 cell lysate.")
![Immunocytochemistry/Immunofluorescence: ACAT1 Antibody (15E5) [NBP2-22565] - Analysis of ACAT1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACAT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/ACAT1-Antibody-15E5-Immunocytochemistry-Immunofluorescence-NBP2-22565-img0002.jpg)
![Western Blot: ACAT1 Antibody (15E5) [NBP2-22565] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/ACAT1-Antibody-15E5-Western-Blot-NBP2-22565-img0001.jpg)
![Western Blot: SR-BI Antibody [NBP2-99690] - Anti-SR-BI rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 60 kDa. Observed band size: 81 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/SR-BI-Antibody-Western-Blot-NBP2-99690-img0007.jpg "Western Blot: SR-BI Antibody [NBP2-99690] - Anti-SR-BI rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 60 kDa. Observed band size: 81 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: SR-BI Antibody [NBP2-99690] - SR-BI was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. 1 ul anti-SR-BI rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-SR-BI rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 60 kDa. Observed band size: 81 kDa](http://images.novusbio.com/fullsize/SR-BI-Antibody-Immunoprecipitation-NBP2-99690-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: SR-BI Antibody [NBP2-99690] - Immunofluorescence staining of SR-BI in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99690 antibody (1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane and cytoplasm.](http://images.novusbio.com/fullsize/nbp2-99690_rabbit-polyclonal-sr-bi-antibody-23520251449239.png)