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![Western Blot: HO-1/HMOX1/HSP32 Antibody (PSH02-76) [NBP3-32420] - Western blot analysis of HO-1/HMOX1/HSP32 on different lysates with Rabbit anti-HO-1/HMOX1/HSP32 antibody (NBP3-32420) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: RAW264.7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32420) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32420_rabbit-ho-1-hmox1-hsp32-mab-psh02-76-27820241524263.jpg "Western Blot: HO-1/HMOX1/HSP32 Antibody (PSH02-76) [NBP3-32420] - Western blot analysis of HO-1/HMOX1/HSP32 on different lysates with Rabbit anti-HO-1/HMOX1/HSP32 antibody (NBP3-32420) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: RAW264.7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32420) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HO-1/HMOX1/HSP32 Antibody (PSH02-76) [NBP3-32420] - Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue with Rabbit anti-HO-1/HMOX1/HSP32 antibody (NBP3-32420) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32420) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32420_rabbit-ho-1-hmox1-hsp32-mab-psh02-76-298202414262647.jpg)
![Immunocytochemistry/ Immunofluorescence: HO-1/HMOX1/HSP32 Antibody (PSH02-76) [NBP3-32420] - Immunocytochemistry analysis of A549 cells labeling HO-1/HMOX1/HSP32 with Rabbit anti-HO-1/HMOX1/HSP32 antibody (NBP3-32420) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HO-1/HMOX1/HSP32 antibody (NBP3-32420) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32420_rabbit-ho-1-hmox1-hsp32-mab-psh02-76-29202410484883.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (SP08-07) [NBP2-67382] - Analysis of paraffin-embedded mouse liver tissue using anti-HO-1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-SP08-07-Immunohistochemistry-Paraffin-NBP2-67382-img0004.jpg "Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (SP08-07) [NBP2-67382] - Analysis of paraffin-embedded mouse liver tissue using anti-HO-1 antibody. Counter stained with hematoxylin.")
![Flow Cytometry: HO-1/HMOX1/HSP32 Antibody (SP08-07) [NBP2-67382] - Analysis of Jurkat cells with HO-1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-SP08-07-Flow-Cytometry-NBP2-67382-img0001.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (SP08-07) [NBP2-67382] - Analysis of paraffin-embedded human liver tissue using anti-HO-1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-SP08-07-Immunohistochemistry-Paraffin-NBP2-67382-img0002.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (SP08-07) [NBP2-67382] - Analysis of paraffin-embedded human spleen tissue using anti-HO-1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-SP08-07-Immunohistochemistry-Paraffin-NBP2-67382-img0003.jpg)
![Western Blot: iNOS Antibody (PSH03-69) [NBP3-32473] - Western blot analysis of iNOS on different lysates with Rabbit anti-iNOS antibody (NBP3-32473) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 1ug/mL LPS for 24 hours cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 131 kDa Observed band size: 131 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32473) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32473_rabbit-inos-mab-psh03-69-278202415295526.jpg "Western Blot: iNOS Antibody (PSH03-69) [NBP3-32473] - Western blot analysis of iNOS on different lysates with Rabbit anti-iNOS antibody (NBP3-32473) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 1ug/mL LPS for 24 hours cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 131 kDa Observed band size: 131 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32473) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: iNOS Antibody (PSH03-69) [NBP3-32473] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-iNOS antibody (NBP3-32473) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32473) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32473_rabbit-inos-mab-psh03-69-298202414304151.jpg)
![Immunocytochemistry/ Immunofluorescence: iNOS Antibody (PSH03-69) [NBP3-32473] - Immunocytochemistry analysis of RAW264.7 cells treated with or without 1μg/mL LPS for 6 hours labeling iNOS with Rabbit anti-iNOS antibody (NBP3-32473) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-iNOS antibody (NBP3-32473) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32473_rabbit-inos-mab-psh03-69-29202410334018.jpg)
![Flow Cytometry: Insulin R/CD220 Antibody (83-14) [NBP2-81038] - Flow-cytometry using the Insulin R/CD220 Antibody (83-14) [NBP2-81038]. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of 83-14 (NBP2-81038, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Insulin-R-CD220-Antibody-83-14-Flow-Cytometry-NBP2-81038-img0001.jpg "Flow Cytometry: Insulin R/CD220 Antibody (83-14) [NBP2-81038] - Flow-cytometry using the Insulin R/CD220 Antibody (83-14) [NBP2-81038]. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of 83-14 (NBP2-81038, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.")
![Western Blot: PKLR Antibody [NBP1-32314] - Sample (50 ug of whole cell lysate) A: mouse liver 7. 5% SDS PAGE; antibody diluted at 1:500.](http://images.novusbio.com/fullsize/PKLR-Antibody-Western-Blot-NBP1-32314-img0007.jpg "Western Blot: PKLR Antibody [NBP1-32314] - Sample (50 ug of whole cell lysate) A: mouse liver 7. 5% SDS PAGE; antibody diluted at 1:500.")
![Western Blot: PKLR Antibody [NBP1-32314] - 30 ug Raw264. 7 whole cell lysate/extract 7. 5 % SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/PKLR-Antibody-Western-Blot-NBP1-32314-img0008.jpg)
![Western Blot: PKLR Antibody [NBP1-32314] - A. 30 ug K562 whole cell lysate/extract 7. 5 % SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/PKLR-Antibody-Western-Blot-NBP1-32314-img0009.jpg)
![Western Blot: PKLR Antibody [NBP1-32314] - PKLR antibody detects PKLR protein by western blot analysis.A. 30 ug 293T whole cell lysate/extract B. 5 ug whole cell lysate/extract of human PKLR-transfected 293T cells7.5 % SDS-PAGEPKLR antibody (NBP1-32314) dilution: 1:10000](http://images.novusbio.com/fullsize/nbp1-32314_rabbit-polyclonal-pklr-antibody-910202420134088.jpg)
![Immunocytochemistry/Immunofluorescence: PRPS1 Antibody [NBP1-31654] - Analysis of methanol-fixed HeLa, at 1:200 dilution.](http://images.novusbio.com/fullsize/PRPS1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31654-img0006.jpg "Immunocytochemistry/Immunofluorescence: PRPS1 Antibody [NBP1-31654] - Analysis of methanol-fixed HeLa, at 1:200 dilution.")
![Immunohistochemistry-Paraffin: PRPS1 Antibody [NBP1-31654] - Paraffin-embedded SW480 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PRPS1-Antibody-Immunohistochemistry-Paraffin-NBP1-31654-img0004.jpg)
![Immunohistochemistry: Cytochrome P450 17A1 Antibody (5A9) [NBP3-26637] - Image of Cytochrome P450 17A1 Antibody (5A9) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26637_rabbit-cytochrome-p450-17a1-mab-5a9-2722024831378.jpg "Immunohistochemistry: Cytochrome P450 17A1 Antibody (5A9) [NBP3-26637] - Image of Cytochrome P450 17A1 Antibody (5A9) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: Cytochrome P450 17A1 Antibody (5A9) [NBP3-26637] - Positive Western Blot detected in: HepG2 whole cell lysate, Rat Heart whole cell lysate, Rat Brain whole cell lysate. All lanes: Cytochrome P450 17A1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 58 kDa Observed band size: 58 kDa](http://images.novusbio.com/fullsize/nbp3-26637_rabbit-cytochrome-p450-17a1-mab-5a9-28220241140920.jpg)
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 17A1 Antibody (5A9) [NBP3-26637] - Staining of Hela Cells with Cytochrome P450 17A1 Antibody (5A9) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26637_rabbit-cytochrome-p450-17a1-mab-5a9-282202412475149.jpg)
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-44228] - Analysis demonstrates the labeling specificity of the antibodies used for Tissue lysate from hog carotid arteries was analyzed by SDS-PAGE. Nitrocellulose membranes were probed with the corresponding antibodies at the same concentrations used for immunofluorescence labeling. 0.05 (PFK) and 0.2 mg (CAV-1) was loaded into lanes 1 and 2, respectively. Western blot development resulted in identification of the proteins of interest at the expected sizes, demonstrating the labeling specificity of the antibodies. Molecular weights are given. Used with permission from Vallejo and Hardin (2004).](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Western-Blot-NBP2-44228-img0003.jpg "Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-44228] - Analysis demonstrates the labeling specificity of the antibodies used for Tissue lysate from hog carotid arteries was analyzed by SDS-PAGE. Nitrocellulose membranes were probed with the corresponding antibodies at the same concentrations used for immunofluorescence labeling. 0.05 (PFK) and 0.2 mg (CAV-1) was loaded into lanes 1 and 2, respectively. Western blot development resulted in identification of the proteins of interest at the expected sizes, demonstrating the labeling specificity of the antibodies. Molecular weights are given. Used with permission from Vallejo and Hardin (2004).")
![Immunocytochemistry/Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-44228] - Cultures of A7r5 VSM cells (rat aorta) were labeled with antibody and anti-caveolin (CAV)-1. Images from labeled A7r5 cells were acquired separately for PFK fluorescence (green) and CAV-1 fluorescence (red) from each cell. Used with permission from Vallejo and Hardin (2004).](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-44228-img0001.jpg)
![Western Blot: Cytochrome P450 4A Antibody [NBP1-31427] - Sample(30 ug of whole cell lysate) A:A431 B:H1299 7.5% SDS PAGE, antibody diluted at 1:100.](http://images.novusbio.com/fullsize/Cytochrome-P450-4A-Antibody-Western-Blot-NBP1-31427-img0005.jpg "Western Blot: Cytochrome P450 4A Antibody [NBP1-31427] - Sample(30 ug of whole cell lysate) A:A431 B:H1299 7.5% SDS PAGE, antibody diluted at 1:100.")
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 4A Antibody [NBP1-31427] - Analysis of HeLa, using NBP1-31427 at 1:200 dilution.](http://images.novusbio.com/fullsize/Cytochrome-P450-4A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31427-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 4A Antibody [NBP1-31427] - Paraffin-embedded mixed OVCA, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Cytochrome-P450-4A-Antibody-Immunohistochemistry-Paraffin-NBP1-31427-img0004.jpg)
![Western Blot: CES2 Antibody [NBP2-15864] - Sample (30 ug of whole cell lysate) A: HepG2 7. 5% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/fullsize/CES2-Antibody-Western-Blot-NBP2-15864-img0001.jpg "Western Blot: CES2 Antibody [NBP2-15864] - Sample (30 ug of whole cell lysate) A: HepG2 7. 5% SDS PAGE gel, diluted at 1:2000.")
![Immunocytochemistry/Immunofluorescence: CES2 Antibody [NBP2-15864] - Detection of CES2 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in iced-cold MeOH for 5 min. Green: CES2 protein stained by CES2 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/CES2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15864-img0003.jpg)
![Immunohistochemistry-Paraffin: CES2 Antibody [NBP2-15864] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/CES2-Antibody-Immunohistochemistry-Paraffin-NBP2-15864-img0002.jpg)
![Western Blot: Phosphodiesterase 4D Antibody [NBP1-31131] - Sample (30 ug of whole cell lysate) A: A431 B: HeLa 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Phosphodiesterase-4D-Antibody-Western-Blot-NBP1-31131-img0004.jpg "Western Blot: Phosphodiesterase 4D Antibody [NBP1-31131] - Sample (30 ug of whole cell lysate) A: A431 B: HeLa 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Phosphodiesterase 4D Antibody [NBP1-31131] - Paraformaldehyde-fixed A431, using PDE4D antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:500.](http://images.novusbio.com/fullsize/Phosphodiesterase-4D-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31131-img0002.jpg)
![Immunohistochemistry-Paraffin: Phosphodiesterase 4D Antibody [NBP1-31131] - Paraffin-embedded H441 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Phosphodiesterase-4D-Antibody-Immunohistochemistry-Paraffin-NBP1-31131-img0003.jpg)
![Immunohistochemistry-Paraffin: P2Y2 Antibody [NBP3-12229] - 1:100 dilution in IHC blocking buffer. DAB (brown) staining and hematoxyllin QS (blue) counterstrain. 20x magnification on leica DM4000. FFPE section](http://images.novusbio.com/fullsize/P2Y2-Antibody-Immunohistochemistry-Paraffin-NBP3-12229-img0001.jpg "Immunohistochemistry-Paraffin: P2Y2 Antibody [NBP3-12229] - 1:100 dilution in IHC blocking buffer. DAB (brown) staining and hematoxyllin QS (blue) counterstrain. 20x magnification on leica DM4000. FFPE section")